Amino acid dipepetide frequency for Roseburia sp. 831b

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.286AlaAla: 6.286 ± 0.122
1.007AlaCys: 1.007 ± 0.037
4.467AlaAsp: 4.467 ± 0.084
4.883AlaGlu: 4.883 ± 0.091
2.988AlaPhe: 2.988 ± 0.059
5.674AlaGly: 5.674 ± 0.101
1.041AlaHis: 1.041 ± 0.036
5.403AlaIle: 5.403 ± 0.086
5.759AlaLys: 5.759 ± 0.092
6.387AlaLeu: 6.387 ± 0.095
2.309AlaMet: 2.309 ± 0.052
2.947AlaAsn: 2.947 ± 0.062
1.801AlaPro: 1.801 ± 0.049
2.334AlaGln: 2.334 ± 0.057
2.55AlaArg: 2.55 ± 0.053
4.099AlaSer: 4.099 ± 0.076
3.77AlaThr: 3.77 ± 0.064
5.605AlaVal: 5.605 ± 0.084
0.549AlaTrp: 0.549 ± 0.023
2.908AlaTyr: 2.908 ± 0.055
0.0AlaXaa: 0.0 ± 0.0
Cys
0.94CysAla: 0.94 ± 0.034
0.29CysCys: 0.29 ± 0.02
0.83CysAsp: 0.83 ± 0.031
1.035CysGlu: 1.035 ± 0.035
0.622CysPhe: 0.622 ± 0.027
1.386CysGly: 1.386 ± 0.04
0.285CysHis: 0.285 ± 0.02
1.105CysIle: 1.105 ± 0.037
0.992CysLys: 0.992 ± 0.033
1.072CysLeu: 1.072 ± 0.036
0.503CysMet: 0.503 ± 0.024
0.668CysAsn: 0.668 ± 0.028
0.54CysPro: 0.54 ± 0.024
0.436CysGln: 0.436 ± 0.022
0.594CysArg: 0.594 ± 0.029
0.892CysSer: 0.892 ± 0.031
0.674CysThr: 0.674 ± 0.028
0.987CysVal: 0.987 ± 0.034
0.121CysTrp: 0.121 ± 0.012
0.614CysTyr: 0.614 ± 0.026
0.0CysXaa: 0.0 ± 0.0
Asp
4.708AspAla: 4.708 ± 0.097
0.825AspCys: 0.825 ± 0.031
3.234AspAsp: 3.234 ± 0.079
5.089AspGlu: 5.089 ± 0.075
2.909AspPhe: 2.909 ± 0.062
4.421AspGly: 4.421 ± 0.092
0.799AspHis: 0.799 ± 0.033
4.509AspIle: 4.509 ± 0.08
3.57AspLys: 3.57 ± 0.066
4.496AspLeu: 4.496 ± 0.068
1.926AspMet: 1.926 ± 0.046
2.301AspAsn: 2.301 ± 0.053
1.468AspPro: 1.468 ± 0.041
1.262AspGln: 1.262 ± 0.038
1.943AspArg: 1.943 ± 0.049
3.209AspSer: 3.209 ± 0.063
3.374AspThr: 3.374 ± 0.068
4.258AspVal: 4.258 ± 0.078
0.557AspTrp: 0.557 ± 0.024
2.951AspTyr: 2.951 ± 0.062
0.0AspXaa: 0.0 ± 0.0
Glu
5.441GluAla: 5.441 ± 0.086
0.897GluCys: 0.897 ± 0.035
4.603GluAsp: 4.603 ± 0.076
7.96GluGlu: 7.96 ± 0.115
2.761GluPhe: 2.761 ± 0.053
4.238GluGly: 4.238 ± 0.076
1.517GluHis: 1.517 ± 0.045
5.801GluIle: 5.801 ± 0.081
7.771GluLys: 7.771 ± 0.104
7.048GluLeu: 7.048 ± 0.109
2.6GluMet: 2.6 ± 0.055
4.469GluAsn: 4.469 ± 0.077
1.799GluPro: 1.799 ± 0.045
3.562GluGln: 3.562 ± 0.07
3.426GluArg: 3.426 ± 0.073
3.669GluSer: 3.669 ± 0.072
4.129GluThr: 4.129 ± 0.072
4.957GluVal: 4.957 ± 0.078
0.696GluTrp: 0.696 ± 0.025
3.229GluTyr: 3.229 ± 0.063
0.0GluXaa: 0.0 ± 0.0
Phe
3.013PheAla: 3.013 ± 0.057
0.804PheCys: 0.804 ± 0.034
2.615PheAsp: 2.615 ± 0.055
2.989PheGlu: 2.989 ± 0.057
2.059PhePhe: 2.059 ± 0.053
2.848PheGly: 2.848 ± 0.066
0.879PheHis: 0.879 ± 0.032
2.593PheIle: 2.593 ± 0.056
2.024PheLys: 2.024 ± 0.052
4.212PheLeu: 4.212 ± 0.078
1.151PheMet: 1.151 ± 0.037
1.471PheAsn: 1.471 ± 0.047
1.296PhePro: 1.296 ± 0.038
1.398PheGln: 1.398 ± 0.038
1.467PheArg: 1.467 ± 0.045
3.047PheSer: 3.047 ± 0.054
2.335PheThr: 2.335 ± 0.05
3.025PheVal: 3.025 ± 0.062
0.453PheTrp: 0.453 ± 0.02
1.933PheTyr: 1.933 ± 0.05
0.0PheXaa: 0.0 ± 0.0
Gly
4.716GlyAla: 4.716 ± 0.086
1.141GlyCys: 1.141 ± 0.034
3.431GlyAsp: 3.431 ± 0.071
4.527GlyGlu: 4.527 ± 0.076
3.039GlyPhe: 3.039 ± 0.051
4.079GlyGly: 4.079 ± 0.086
1.113GlyHis: 1.113 ± 0.033
5.995GlyIle: 5.995 ± 0.093
5.561GlyLys: 5.561 ± 0.077
5.147GlyLeu: 5.147 ± 0.079
2.455GlyMet: 2.455 ± 0.058
3.324GlyAsn: 3.324 ± 0.076
1.113GlyPro: 1.113 ± 0.039
1.925GlyGln: 1.925 ± 0.05
2.465GlyArg: 2.465 ± 0.055
3.621GlySer: 3.621 ± 0.068
4.3GlyThr: 4.3 ± 0.099
4.867GlyVal: 4.867 ± 0.083
0.61GlyTrp: 0.61 ± 0.026
3.248GlyTyr: 3.248 ± 0.061
0.0GlyXaa: 0.0 ± 0.0
His
1.19HisAla: 1.19 ± 0.033
0.29HisCys: 0.29 ± 0.016
0.928HisAsp: 0.928 ± 0.038
1.129HisGlu: 1.129 ± 0.039
0.958HisPhe: 0.958 ± 0.035
1.149HisGly: 1.149 ± 0.036
0.402HisHis: 0.402 ± 0.027
1.311HisIle: 1.311 ± 0.039
0.997HisLys: 0.997 ± 0.03
1.48HisLeu: 1.48 ± 0.045
0.521HisMet: 0.521 ± 0.024
0.771HisAsn: 0.771 ± 0.031
0.854HisPro: 0.854 ± 0.031
0.575HisGln: 0.575 ± 0.025
0.687HisArg: 0.687 ± 0.025
0.854HisSer: 0.854 ± 0.031
0.939HisThr: 0.939 ± 0.039
1.102HisVal: 1.102 ± 0.031
0.146HisTrp: 0.146 ± 0.013
0.808HisTyr: 0.808 ± 0.031
0.0HisXaa: 0.0 ± 0.0
Ile
5.86IleAla: 5.86 ± 0.094
1.321IleCys: 1.321 ± 0.036
4.14IleAsp: 4.14 ± 0.082
5.373IleGlu: 5.373 ± 0.091
2.957IlePhe: 2.957 ± 0.064
5.084IleGly: 5.084 ± 0.079
1.304IleHis: 1.304 ± 0.037
4.615IleIle: 4.615 ± 0.089
4.249IleLys: 4.249 ± 0.074
6.967IleLeu: 6.967 ± 0.11
1.826IleMet: 1.826 ± 0.046
2.912IleAsn: 2.912 ± 0.055
2.933IlePro: 2.933 ± 0.05
2.527IleGln: 2.527 ± 0.052
3.198IleArg: 3.198 ± 0.064
4.973IleSer: 4.973 ± 0.083
4.207IleThr: 4.207 ± 0.069
5.279IleVal: 5.279 ± 0.086
0.606IleTrp: 0.606 ± 0.026
2.751IleTyr: 2.751 ± 0.057
0.0IleXaa: 0.0 ± 0.0
Lys
5.015LysAla: 5.015 ± 0.082
0.775LysCys: 0.775 ± 0.03
4.25LysAsp: 4.25 ± 0.07
8.259LysGlu: 8.259 ± 0.114
2.007LysPhe: 2.007 ± 0.044
4.187LysGly: 4.187 ± 0.078
1.15LysHis: 1.15 ± 0.039
5.343LysIle: 5.343 ± 0.071
7.046LysLys: 7.046 ± 0.099
5.658LysLeu: 5.658 ± 0.08
2.532LysMet: 2.532 ± 0.053
3.86LysAsn: 3.86 ± 0.064
1.928LysPro: 1.928 ± 0.05
2.78LysGln: 2.78 ± 0.064
3.108LysArg: 3.108 ± 0.066
3.605LysSer: 3.605 ± 0.062
3.865LysThr: 3.865 ± 0.08
4.618LysVal: 4.618 ± 0.084
0.631LysTrp: 0.631 ± 0.03
2.894LysTyr: 2.894 ± 0.059
0.0LysXaa: 0.0 ± 0.0
Leu
6.493LeuAla: 6.493 ± 0.089
1.415LeuCys: 1.415 ± 0.043
4.997LeuAsp: 4.997 ± 0.082
6.79LeuGlu: 6.79 ± 0.117
3.756LeuPhe: 3.756 ± 0.077
5.613LeuGly: 5.613 ± 0.098
1.523LeuHis: 1.523 ± 0.044
5.647LeuIle: 5.647 ± 0.1
6.164LeuLys: 6.164 ± 0.096
8.461LeuLeu: 8.461 ± 0.147
2.419LeuMet: 2.419 ± 0.053
3.97LeuAsn: 3.97 ± 0.068
3.118LeuPro: 3.118 ± 0.062
2.967LeuGln: 2.967 ± 0.059
3.416LeuArg: 3.416 ± 0.071
6.167LeuSer: 6.167 ± 0.095
4.831LeuThr: 4.831 ± 0.076
5.608LeuVal: 5.608 ± 0.083
0.661LeuTrp: 0.661 ± 0.027
3.29LeuTyr: 3.29 ± 0.057
0.0LeuXaa: 0.0 ± 0.0
Met
2.218MetAla: 2.218 ± 0.052
0.379MetCys: 0.379 ± 0.017
1.914MetAsp: 1.914 ± 0.042
2.747MetGlu: 2.747 ± 0.062
1.069MetPhe: 1.069 ± 0.033
1.926MetGly: 1.926 ± 0.05
0.462MetHis: 0.462 ± 0.023
2.186MetIle: 2.186 ± 0.056
2.64MetLys: 2.64 ± 0.058
2.59MetLeu: 2.59 ± 0.053
0.974MetMet: 0.974 ± 0.038
1.592MetAsn: 1.592 ± 0.04
1.095MetPro: 1.095 ± 0.036
1.31MetGln: 1.31 ± 0.037
1.222MetArg: 1.222 ± 0.042
1.833MetSer: 1.833 ± 0.044
1.8MetThr: 1.8 ± 0.046
1.921MetVal: 1.921 ± 0.047
0.216MetTrp: 0.216 ± 0.016
0.925MetTyr: 0.925 ± 0.031
0.0MetXaa: 0.0 ± 0.0
Asn
3.435AsnAla: 3.435 ± 0.07
0.632AsnCys: 0.632 ± 0.028
2.285AsnAsp: 2.285 ± 0.051
3.296AsnGlu: 3.296 ± 0.068
1.731AsnPhe: 1.731 ± 0.046
3.665AsnGly: 3.665 ± 0.081
0.962AsnHis: 0.962 ± 0.033
3.451AsnIle: 3.451 ± 0.057
2.625AsnLys: 2.625 ± 0.059
4.03AsnLeu: 4.03 ± 0.065
1.42AsnMet: 1.42 ± 0.037
1.944AsnAsn: 1.944 ± 0.056
1.946AsnPro: 1.946 ± 0.049
1.94AsnGln: 1.94 ± 0.051
2.009AsnArg: 2.009 ± 0.042
2.409AsnSer: 2.409 ± 0.06
2.449AsnThr: 2.449 ± 0.06
3.29AsnVal: 3.29 ± 0.056
0.424AsnTrp: 0.424 ± 0.024
2.029AsnTyr: 2.029 ± 0.057
0.0AsnXaa: 0.0 ± 0.0
Pro
2.055ProAla: 2.055 ± 0.058
0.431ProCys: 0.431 ± 0.024
2.107ProAsp: 2.107 ± 0.043
2.771ProGlu: 2.771 ± 0.059
1.42ProPhe: 1.42 ± 0.04
1.935ProGly: 1.935 ± 0.05
0.499ProHis: 0.499 ± 0.021
2.214ProIle: 2.214 ± 0.048
2.018ProLys: 2.018 ± 0.046
2.462ProLeu: 2.462 ± 0.053
0.863ProMet: 0.863 ± 0.032
1.235ProAsn: 1.235 ± 0.037
0.595ProPro: 0.595 ± 0.032
1.059ProGln: 1.059 ± 0.039
0.801ProArg: 0.801 ± 0.033
1.638ProSer: 1.638 ± 0.043
1.523ProThr: 1.523 ± 0.047
2.515ProVal: 2.515 ± 0.052
0.29ProTrp: 0.29 ± 0.019
1.463ProTyr: 1.463 ± 0.042
0.0ProXaa: 0.0 ± 0.0
Gln
2.409GlnAla: 2.409 ± 0.054
0.364GlnCys: 0.364 ± 0.02
1.789GlnAsp: 1.789 ± 0.045
3.097GlnGlu: 3.097 ± 0.065
1.308GlnPhe: 1.308 ± 0.035
1.903GlnGly: 1.903 ± 0.048
0.48GlnHis: 0.48 ± 0.023
2.777GlnIle: 2.777 ± 0.059
3.191GlnLys: 3.191 ± 0.055
2.948GlnLeu: 2.948 ± 0.062
1.29GlnMet: 1.29 ± 0.039
2.011GlnAsn: 2.011 ± 0.055
0.87GlnPro: 0.87 ± 0.039
1.334GlnGln: 1.334 ± 0.052
1.299GlnArg: 1.299 ± 0.041
1.689GlnSer: 1.689 ± 0.045
1.882GlnThr: 1.882 ± 0.048
2.251GlnVal: 2.251 ± 0.049
0.27GlnTrp: 0.27 ± 0.02
1.529GlnTyr: 1.529 ± 0.042
0.0GlnXaa: 0.0 ± 0.0
Arg
2.388ArgAla: 2.388 ± 0.047
0.504ArgCys: 0.504 ± 0.023
1.981ArgAsp: 1.981 ± 0.053
3.419ArgGlu: 3.419 ± 0.073
1.646ArgPhe: 1.646 ± 0.038
2.106ArgGly: 2.106 ± 0.051
0.702ArgHis: 0.702 ± 0.029
3.189ArgIle: 3.189 ± 0.068
3.435ArgLys: 3.435 ± 0.068
3.312ArgLeu: 3.312 ± 0.069
1.462ArgMet: 1.462 ± 0.043
1.959ArgAsn: 1.959 ± 0.047
1.103ArgPro: 1.103 ± 0.039
1.419ArgGln: 1.419 ± 0.04
1.71ArgArg: 1.71 ± 0.049
1.818ArgSer: 1.818 ± 0.042
1.946ArgThr: 1.946 ± 0.048
2.409ArgVal: 2.409 ± 0.061
0.322ArgTrp: 0.322 ± 0.019
1.768ArgTyr: 1.768 ± 0.049
0.0ArgXaa: 0.0 ± 0.0
Ser
3.985SerAla: 3.985 ± 0.075
0.807SerCys: 0.807 ± 0.029
3.584SerAsp: 3.584 ± 0.068
4.023SerGlu: 4.023 ± 0.066
2.844SerPhe: 2.844 ± 0.063
4.38SerGly: 4.38 ± 0.09
0.937SerHis: 0.937 ± 0.033
4.283SerIle: 4.283 ± 0.085
3.846SerLys: 3.846 ± 0.063
5.028SerLeu: 5.028 ± 0.079
1.902SerMet: 1.902 ± 0.046
2.604SerAsn: 2.604 ± 0.056
1.455SerPro: 1.455 ± 0.036
1.877SerGln: 1.877 ± 0.048
2.071SerArg: 2.071 ± 0.044
3.508SerSer: 3.508 ± 0.079
3.008SerThr: 3.008 ± 0.069
4.252SerVal: 4.252 ± 0.067
0.524SerTrp: 0.524 ± 0.021
2.808SerTyr: 2.808 ± 0.064
0.0SerXaa: 0.0 ± 0.0
Thr
4.022ThrAla: 4.022 ± 0.082
0.722ThrCys: 0.722 ± 0.027
3.355ThrAsp: 3.355 ± 0.064
3.995ThrGlu: 3.995 ± 0.066
2.225ThrPhe: 2.225 ± 0.052
4.365ThrGly: 4.365 ± 0.075
0.88ThrHis: 0.88 ± 0.035
4.293ThrIle: 4.293 ± 0.067
3.667ThrLys: 3.667 ± 0.066
4.717ThrLeu: 4.717 ± 0.082
1.592ThrMet: 1.592 ± 0.036
2.508ThrAsn: 2.508 ± 0.062
2.051ThrPro: 2.051 ± 0.06
1.709ThrGln: 1.709 ± 0.042
1.81ThrArg: 1.81 ± 0.045
3.225ThrSer: 3.225 ± 0.067
3.351ThrThr: 3.351 ± 0.089
4.365ThrVal: 4.365 ± 0.089
0.48ThrTrp: 0.48 ± 0.024
2.351ThrTyr: 2.351 ± 0.062
0.0ThrXaa: 0.0 ± 0.0
Val
5.245ValAla: 5.245 ± 0.084
1.21ValCys: 1.21 ± 0.033
3.965ValAsp: 3.965 ± 0.065
5.007ValGlu: 5.007 ± 0.079
2.965ValPhe: 2.965 ± 0.064
4.313ValGly: 4.313 ± 0.086
1.11ValHis: 1.11 ± 0.035
5.095ValIle: 5.095 ± 0.076
4.838ValLys: 4.838 ± 0.068
6.495ValLeu: 6.495 ± 0.095
1.923ValMet: 1.923 ± 0.043
2.979ValAsn: 2.979 ± 0.058
2.31ValPro: 2.31 ± 0.055
1.882ValGln: 1.882 ± 0.041
2.61ValArg: 2.61 ± 0.063
4.714ValSer: 4.714 ± 0.073
4.583ValThr: 4.583 ± 0.094
5.344ValVal: 5.344 ± 0.095
0.614ValTrp: 0.614 ± 0.03
2.839ValTyr: 2.839 ± 0.058
0.0ValXaa: 0.0 ± 0.0
Trp
0.46TrpAla: 0.46 ± 0.025
0.142TrpCys: 0.142 ± 0.012
0.52TrpAsp: 0.52 ± 0.025
0.677TrpGlu: 0.677 ± 0.028
0.415TrpPhe: 0.415 ± 0.022
0.534TrpGly: 0.534 ± 0.026
0.162TrpHis: 0.162 ± 0.013
0.631TrpIle: 0.631 ± 0.027
0.699TrpLys: 0.699 ± 0.029
0.783TrpLeu: 0.783 ± 0.027
0.318TrpMet: 0.318 ± 0.02
0.586TrpAsn: 0.586 ± 0.026
0.181TrpPro: 0.181 ± 0.015
0.336TrpGln: 0.336 ± 0.019
0.333TrpArg: 0.333 ± 0.019
0.51TrpSer: 0.51 ± 0.029
0.365TrpThr: 0.365 ± 0.021
0.533TrpVal: 0.533 ± 0.024
0.105TrpTrp: 0.105 ± 0.015
0.405TrpTyr: 0.405 ± 0.022
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.886TyrAla: 2.886 ± 0.058
0.632TyrCys: 0.632 ± 0.029
2.813TyrAsp: 2.813 ± 0.075
3.509TyrGlu: 3.509 ± 0.068
1.93TyrPhe: 1.93 ± 0.046
2.869TyrGly: 2.869 ± 0.055
0.883TyrHis: 0.883 ± 0.033
2.64TyrIle: 2.64 ± 0.058
2.418TyrLys: 2.418 ± 0.057
4.017TyrLeu: 4.017 ± 0.075
1.031TyrMet: 1.031 ± 0.033
1.863TyrAsn: 1.863 ± 0.052
1.435TyrPro: 1.435 ± 0.043
2.06TyrGln: 2.06 ± 0.048
1.879TyrArg: 1.879 ± 0.044
2.249TyrSer: 2.249 ± 0.047
2.352TyrThr: 2.352 ± 0.065
2.857TyrVal: 2.857 ± 0.065
0.42TyrTrp: 0.42 ± 0.02
1.914TyrTyr: 1.914 ± 0.053
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2925 proteins (949512 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski