Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Bradyrhizobiaceae; Nitrobacter; Nitrobacter winogradskyi

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2980 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q3SU20|Q3SU20_NITWN Uncharacterized protein OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255) OX=323098 GN=Nwi_0959 PE=4 SV=1
MM1 pKa = 7.51ISGGITEE8 pKa = 5.3IIWHH12 pKa = 5.79FAGYY16 pKa = 10.4LRR18 pKa = 11.84IFDD21 pKa = 5.01DD22 pKa = 3.21IARR25 pKa = 11.84FRR27 pKa = 11.84IAHH30 pKa = 6.83DD31 pKa = 3.29EE32 pKa = 3.93GAYY35 pKa = 9.99RR36 pKa = 11.84SHH38 pKa = 6.64TEE40 pKa = 3.93DD41 pKa = 3.35YY42 pKa = 7.6TTNRR46 pKa = 11.84PQFAFTPIDD55 pKa = 3.75DD56 pKa = 4.32EE57 pKa = 4.36MSSRR61 pKa = 11.84PVAIQNPSVMGGAGHH76 pKa = 7.26HH77 pKa = 6.79APTSHH82 pKa = 7.3LSPVPDD88 pKa = 3.64VGPDD92 pKa = 3.41KK93 pKa = 10.74INPIHH98 pKa = 7.13ASGFLPPMSPGAGGGVGGSDD118 pKa = 3.84VIKK121 pKa = 10.67VVYY124 pKa = 9.9QEE126 pKa = 4.78EE127 pKa = 4.97GAQSEE132 pKa = 4.68VQTRR136 pKa = 11.84QFNHH140 pKa = 6.39MYY142 pKa = 11.05DD143 pKa = 3.32NDD145 pKa = 4.32ALVGTQDD152 pKa = 3.5SNLLIALRR160 pKa = 11.84AEE162 pKa = 4.1SDD164 pKa = 3.35HH165 pKa = 7.19AIATMIDD172 pKa = 3.76GADD175 pKa = 3.34SAIPVDD181 pKa = 3.32WRR183 pKa = 11.84VPSNTADD190 pKa = 3.34LPAFVVKK197 pKa = 10.09HH198 pKa = 5.3HH199 pKa = 6.17QALADD204 pKa = 4.09RR205 pKa = 11.84DD206 pKa = 4.16GAPDD210 pKa = 3.58PHH212 pKa = 7.87SVQQGYY218 pKa = 8.26YY219 pKa = 9.98VNGVLQDD226 pKa = 3.64PALRR230 pKa = 11.84PPDD233 pKa = 3.6QLSASDD239 pKa = 4.21PAPVPDD245 pKa = 5.05FGSGPGQWALDD256 pKa = 3.88GGNTATNGAYY266 pKa = 9.39IVDD269 pKa = 4.16LTGSARR275 pKa = 11.84TMLVVGDD282 pKa = 4.0YY283 pKa = 10.67YY284 pKa = 10.34KK285 pKa = 11.04TNAIVQTNSYY295 pKa = 9.02MDD297 pKa = 4.06NDD299 pKa = 3.94HH300 pKa = 5.82VTVGGSGAGITTGGNQATNVADD322 pKa = 4.81FVQNPGVYY330 pKa = 10.14EE331 pKa = 4.26SMPSSFAGPNWSVDD345 pKa = 3.73VVKK348 pKa = 10.87GDD350 pKa = 3.61YY351 pKa = 10.71FNIRR355 pKa = 11.84ILLQSNYY362 pKa = 10.45LSDD365 pKa = 4.35NDD367 pKa = 3.39VTMQSSSSTHH377 pKa = 5.65YY378 pKa = 10.06EE379 pKa = 3.44VHH381 pKa = 6.68AGQNEE386 pKa = 4.24LEE388 pKa = 4.24NFVLINDD395 pKa = 3.71GNFNYY400 pKa = 10.5DD401 pKa = 3.54VIVVGGSYY409 pKa = 10.49HH410 pKa = 5.73GMNVIFQNNILYY422 pKa = 10.22NNDD425 pKa = 2.97MVMMTGDD432 pKa = 3.68GVEE435 pKa = 4.33PGQTVSTGGNQLTNTATIEE454 pKa = 4.36SYY456 pKa = 11.07GGADD460 pKa = 3.49TQPWNSDD467 pKa = 3.29LDD469 pKa = 4.15SLIHH473 pKa = 6.38NISGGATEE481 pKa = 5.28LDD483 pKa = 3.56PSFGEE488 pKa = 4.56LIAGNGGTLNILYY501 pKa = 8.72ITGDD505 pKa = 3.91YY506 pKa = 10.92YY507 pKa = 11.35DD508 pKa = 5.48VNAIWQTNIVADD520 pKa = 4.02ANMAIQLQGSPSEE533 pKa = 4.34VTAGYY538 pKa = 10.26YY539 pKa = 9.96GDD541 pKa = 5.23SMTTQNVATGGNVLSNDD558 pKa = 3.23AVIVDD563 pKa = 3.74VGSTNIHH570 pKa = 6.06VNGQVYY576 pKa = 10.47GDD578 pKa = 4.65SILIQANLVEE588 pKa = 4.51EE589 pKa = 5.59DD590 pKa = 3.78SDD592 pKa = 4.64QVTQTDD598 pKa = 3.75PQALVPEE605 pKa = 4.89VIAFIGPNDD614 pKa = 3.89TNDD617 pKa = 3.53NSDD620 pKa = 3.97TQPHH624 pKa = 5.99ATTSIPHH631 pKa = 7.69DD632 pKa = 3.9DD633 pKa = 3.45PMANILHH640 pKa = 6.58

Molecular weight: 68.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q3SP89|Q3SP89_NITWN Cytochrome c class IC OS=Nitrobacter winogradskyi (strain ATCC 25391 / DSM 10237 / CIP 104748 / NCIMB 11846 / Nb-255) OX=323098 GN=Nwi_2649 PE=4 SV=1
MM1 pKa = 7.6LLKK4 pKa = 10.59KK5 pKa = 10.27ILGFAALGALLFFAAPAQQAQAATPVSPGIVAGQSGAGKK44 pKa = 8.85LTTNVQWGHH53 pKa = 4.43HH54 pKa = 5.13HH55 pKa = 6.0HH56 pKa = 6.25RR57 pKa = 11.84HH58 pKa = 4.67WRR60 pKa = 11.84HH61 pKa = 4.36QRR63 pKa = 11.84HH64 pKa = 5.86HH65 pKa = 6.13RR66 pKa = 11.84RR67 pKa = 11.84HH68 pKa = 5.08WRR70 pKa = 11.84NHH72 pKa = 3.53RR73 pKa = 11.84HH74 pKa = 6.13HH75 pKa = 6.26RR76 pKa = 11.84HH77 pKa = 3.84NWNRR81 pKa = 11.84HH82 pKa = 3.67HH83 pKa = 6.76HH84 pKa = 5.65RR85 pKa = 11.84HH86 pKa = 5.23HH87 pKa = 5.99HH88 pKa = 5.19HH89 pKa = 6.09HH90 pKa = 5.82RR91 pKa = 11.84HH92 pKa = 4.02WRR94 pKa = 3.45

Molecular weight: 11.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2980

0

2980

923695

41

2002

310.0

33.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.204 ± 0.067

0.834 ± 0.015

5.876 ± 0.032

5.489 ± 0.039

3.659 ± 0.031

8.203 ± 0.046

2.062 ± 0.022

5.323 ± 0.031

3.57 ± 0.035

9.565 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.367 ± 0.021

2.771 ± 0.027

5.294 ± 0.033

3.121 ± 0.027

7.868 ± 0.047

5.761 ± 0.033

5.348 ± 0.033

7.243 ± 0.035

1.275 ± 0.019

2.167 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski