Kamptonema sp. PCC 6506

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales; Microcoleaceae; Kamptonema

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5822 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D8FZH9|D8FZH9_9CYAN tRNA-specific adenosine deaminase OS=Kamptonema sp. PCC 6506 OX=272129 GN=tadA PE=3 SV=1
MM1 pKa = 7.6EE2 pKa = 5.0EE3 pKa = 3.85WQKK6 pKa = 11.67SFVAVLEE13 pKa = 4.27TAVAEE18 pKa = 4.25VEE20 pKa = 4.38KK21 pKa = 10.99FFMDD25 pKa = 3.48ASEE28 pKa = 4.66EE29 pKa = 3.95FAEE32 pKa = 4.57MLDD35 pKa = 3.7SLAKK39 pKa = 10.22FSEE42 pKa = 4.48EE43 pKa = 3.69VTEE46 pKa = 4.78EE47 pKa = 3.87IQNTVIDD54 pKa = 4.84DD55 pKa = 3.81FDD57 pKa = 4.81NYY59 pKa = 10.46FNEE62 pKa = 3.98LLEE65 pKa = 4.75PIIEE69 pKa = 4.32VFRR72 pKa = 11.84EE73 pKa = 3.73LDD75 pKa = 3.55PEE77 pKa = 4.2VNEE80 pKa = 4.03IDD82 pKa = 3.99FSIVTYY88 pKa = 9.62VEE90 pKa = 4.32PSTIQQPACRR100 pKa = 11.84GCSNYY105 pKa = 9.79HH106 pKa = 3.85GHH108 pKa = 7.16IYY110 pKa = 10.52GGNLLVCAMHH120 pKa = 6.59PSGVEE125 pKa = 4.08SEE127 pKa = 4.8SCPDD131 pKa = 3.03WEE133 pKa = 5.17AEE135 pKa = 4.11RR136 pKa = 11.84EE137 pKa = 4.21

Molecular weight: 15.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D8G867|D8G867_9CYAN Thymidylat_synt domain-containing protein OS=Kamptonema sp. PCC 6506 OX=272129 GN=OSCI_3890023 PE=4 SV=1
MM1 pKa = 6.86TQQTLHH7 pKa = 5.03GTSRR11 pKa = 11.84KK12 pKa = 9.46RR13 pKa = 11.84KK14 pKa = 7.05RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TKK26 pKa = 10.37NGQAVISARR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05KK38 pKa = 10.18GRR40 pKa = 11.84HH41 pKa = 4.82RR42 pKa = 11.84LAVV45 pKa = 3.37

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5822

0

5822

1812636

21

3379

311.3

34.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.327 ± 0.034

1.0 ± 0.011

4.936 ± 0.025

6.66 ± 0.04

3.917 ± 0.022

6.811 ± 0.046

1.669 ± 0.017

6.846 ± 0.029

4.853 ± 0.034

10.944 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.878 ± 0.018

4.404 ± 0.034

4.742 ± 0.03

5.019 ± 0.029

5.2 ± 0.03

6.553 ± 0.029

5.495 ± 0.034

6.402 ± 0.026

1.378 ± 0.015

2.966 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski