Nitrosomonas aestuarii

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Nitrosomonadaceae; Nitrosomonas

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3744 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I4DRV1|A0A1I4DRV1_9PROT Condensin subunit ScpA OS=Nitrosomonas aestuarii OX=52441 GN=SAMN05216302_102212 PE=3 SV=1
TT1 pKa = 7.26AGSTGGNTDD10 pKa = 3.0VNDD13 pKa = 4.63IISQITVNAGEE24 pKa = 4.33NSTDD28 pKa = 3.43NNFGEE33 pKa = 4.63VLPPPEE39 pKa = 4.6PASISGFVYY48 pKa = 10.71CDD50 pKa = 4.12DD51 pKa = 4.51NDD53 pKa = 5.49DD54 pKa = 4.6GIKK57 pKa = 10.65DD58 pKa = 3.38AGEE61 pKa = 4.41AGLSNVTIQLLNAAGVVINTTTTAADD87 pKa = 3.62GSYY90 pKa = 10.98SFTGLDD96 pKa = 2.95AGTYY100 pKa = 9.93SVVEE104 pKa = 4.18PTQPAGKK111 pKa = 9.91NDD113 pKa = 3.42GKK115 pKa = 9.34EE116 pKa = 4.19TAGSTGGNTDD126 pKa = 3.0VNDD129 pKa = 4.63IISQITVNAGEE140 pKa = 4.33NSTDD144 pKa = 3.41NNFGEE149 pKa = 4.68VTPTPVIDD157 pKa = 3.19IEE159 pKa = 4.36KK160 pKa = 9.82FVRR163 pKa = 11.84IEE165 pKa = 3.65QPAILFTGAVCEE177 pKa = 4.37VFGKK181 pKa = 9.33PLEE184 pKa = 4.09MTFDD188 pKa = 3.86YY189 pKa = 11.39NIGNTVDD196 pKa = 3.42TDD198 pKa = 3.54QDD200 pKa = 3.61SSKK203 pKa = 11.26AAILTQAGIEE213 pKa = 4.32DD214 pKa = 4.19DD215 pKa = 3.79GKK217 pKa = 11.34SYY219 pKa = 11.35VIVTDD224 pKa = 3.88KK225 pKa = 11.53SNPTDD230 pKa = 3.29SKK232 pKa = 11.54AKK234 pKa = 10.3VFFKK238 pKa = 10.92GYY240 pKa = 10.11VDD242 pKa = 3.6VGSEE246 pKa = 3.96FTASVNADD254 pKa = 2.9ADD256 pKa = 3.91KK257 pKa = 10.82FGSSTYY263 pKa = 9.59VHH265 pKa = 7.02FFDD268 pKa = 6.5DD269 pKa = 4.04NPFDD273 pKa = 3.96AVTGGDD279 pKa = 4.0ALLQSLTYY287 pKa = 8.88HH288 pKa = 6.46TSCSQPIHH296 pKa = 6.82LGDD299 pKa = 3.43MVGNVTLVDD308 pKa = 3.77YY309 pKa = 11.12VGEE312 pKa = 4.89DD313 pKa = 3.35GAAPDD318 pKa = 4.33SVPGVIGPDD327 pKa = 3.1EE328 pKa = 4.67DD329 pKa = 5.51ADD331 pKa = 4.2VPSGPAGEE339 pKa = 4.56TSVDD343 pKa = 3.53TAVFTYY349 pKa = 10.72YY350 pKa = 9.03VTNPGTVSLSNVVVTDD366 pKa = 3.58DD367 pKa = 4.51RR368 pKa = 11.84IAAIEE373 pKa = 4.6FIGGDD378 pKa = 3.87DD379 pKa = 4.27NNDD382 pKa = 3.33GLLDD386 pKa = 4.15PSEE389 pKa = 4.27TWSYY393 pKa = 8.88TASEE397 pKa = 4.19LVMNGLQTNIGTVTGAYY414 pKa = 10.76NNIQVTDD421 pKa = 4.07NDD423 pKa = 4.0PANYY427 pKa = 9.29IGMAAPPAINIEE439 pKa = 4.24KK440 pKa = 8.76YY441 pKa = 10.25VAVAGYY447 pKa = 10.4APAIEE452 pKa = 5.23HH453 pKa = 6.62ICDD456 pKa = 3.61TLGKK460 pKa = 8.12PLSMTFDD467 pKa = 3.7YY468 pKa = 11.23EE469 pKa = 3.84IGNTVDD475 pKa = 3.3TDD477 pKa = 3.54QDD479 pKa = 3.61SSKK482 pKa = 11.26AAILTQAGIEE492 pKa = 4.32DD493 pKa = 4.19DD494 pKa = 3.79GKK496 pKa = 11.19SYY498 pKa = 11.53VVVTDD503 pKa = 3.96KK504 pKa = 11.6SNPTDD509 pKa = 3.29SKK511 pKa = 11.54AKK513 pKa = 10.3VFFKK517 pKa = 10.92GYY519 pKa = 10.11VDD521 pKa = 3.6VGSEE525 pKa = 3.96FTASVNADD533 pKa = 2.93ADD535 pKa = 3.96KK536 pKa = 10.86FGSATYY542 pKa = 10.87VHH544 pKa = 7.04FFDD547 pKa = 6.41DD548 pKa = 4.04NPFDD552 pKa = 3.96AVTGGDD558 pKa = 4.0ALLQSLTYY566 pKa = 8.88HH567 pKa = 6.46TSCSQPINLGDD578 pKa = 3.62TVGNVSLVGYY588 pKa = 9.91AGEE591 pKa = 5.63DD592 pKa = 3.58GTIDD596 pKa = 3.41PSVWFGEE603 pKa = 4.51DD604 pKa = 3.15ADD606 pKa = 4.44TPSGPEE612 pKa = 3.6AAFGTAVDD620 pKa = 4.08FTYY623 pKa = 11.11VVTNPGSTPLANVSVSDD640 pKa = 4.1DD641 pKa = 3.32RR642 pKa = 11.84LSNVTYY648 pKa = 11.15VEE650 pKa = 4.32GDD652 pKa = 3.41TNADD656 pKa = 3.28NKK658 pKa = 10.66LDD660 pKa = 4.22PGEE663 pKa = 3.93AWIYY667 pKa = 7.79TASEE671 pKa = 4.06TALVGQVTNTGIVTATPVDD690 pKa = 3.5ALGNPLGLPEE700 pKa = 4.98VTDD703 pKa = 3.68QDD705 pKa = 3.72DD706 pKa = 3.39ANYY709 pKa = 9.9IVVGGPQIDD718 pKa = 3.51IEE720 pKa = 4.76KK721 pKa = 8.5YY722 pKa = 9.11VAVAGYY728 pKa = 10.29APAIGHH734 pKa = 6.4ICDD737 pKa = 3.74TLGKK741 pKa = 8.12PLSMTFDD748 pKa = 3.76YY749 pKa = 11.46NIGNTVDD756 pKa = 3.42TDD758 pKa = 3.54QDD760 pKa = 3.61SSKK763 pKa = 11.26AAILTQAGIEE773 pKa = 4.32DD774 pKa = 4.19DD775 pKa = 3.79GKK777 pKa = 11.34SYY779 pKa = 11.35VIVTDD784 pKa = 3.88KK785 pKa = 11.53SNPTDD790 pKa = 3.29SKK792 pKa = 11.54AKK794 pKa = 10.3VFFKK798 pKa = 10.92GYY800 pKa = 10.11VDD802 pKa = 3.6VGSEE806 pKa = 3.96FTASVNADD814 pKa = 2.9ADD816 pKa = 3.91KK817 pKa = 10.82FGSSTYY823 pKa = 9.59VHH825 pKa = 7.02FFDD828 pKa = 6.5DD829 pKa = 4.04NPFDD833 pKa = 3.96AVTGGDD839 pKa = 4.0ALLQSLTYY847 pKa = 8.88HH848 pKa = 6.46TSCSQPINLGDD859 pKa = 3.24IVGNVSLVGYY869 pKa = 9.91AGEE872 pKa = 5.63DD873 pKa = 3.58GTIDD877 pKa = 3.41PSVWFGEE884 pKa = 4.47DD885 pKa = 3.38ADD887 pKa = 4.53TGPGPQSLIGDD898 pKa = 3.99NVDD901 pKa = 3.69FTYY904 pKa = 11.13VVTNPGSTPLANVSLTDD921 pKa = 3.23NRR923 pKa = 11.84LNIAPGFEE931 pKa = 3.94GDD933 pKa = 3.51ANQNGLLDD941 pKa = 4.84PGEE944 pKa = 4.07EE945 pKa = 4.21WIYY948 pKa = 9.6TASEE952 pKa = 3.86LAEE955 pKa = 4.47AGQVTNIATVSAIAVDD971 pKa = 3.9AQGQDD976 pKa = 3.01LGLPSVSDD984 pKa = 3.39QDD986 pKa = 3.44AANYY990 pKa = 10.42NGVDD994 pKa = 3.66MLFPGYY1000 pKa = 9.41DD1001 pKa = 3.13ACDD1004 pKa = 3.31TLGKK1008 pKa = 9.77PKK1010 pKa = 10.38ALKK1013 pKa = 9.95FDD1015 pKa = 4.19YY1016 pKa = 10.64EE1017 pKa = 4.14PSVLVNTAQSSDD1029 pKa = 3.08KK1030 pKa = 11.33AKK1032 pKa = 10.52ILFDD1036 pKa = 4.04SGLVDD1041 pKa = 4.2DD1042 pKa = 5.84DD1043 pKa = 4.0GVSFILVTNKK1053 pKa = 10.2SNPTDD1058 pKa = 3.56LGGKK1062 pKa = 7.91EE1063 pKa = 4.01FFMGTVQVGDD1073 pKa = 3.79EE1074 pKa = 5.29FIASTTIAGDD1084 pKa = 3.35KK1085 pKa = 10.36FGSNTYY1091 pKa = 9.47VHH1093 pKa = 6.56VFDD1096 pKa = 6.58DD1097 pKa = 5.2NPFDD1101 pKa = 4.34TNGSNQLLQSAQYY1114 pKa = 8.24HH1115 pKa = 5.44TSCSQPIHH1123 pKa = 7.13LGDD1126 pKa = 3.59QLGDD1130 pKa = 3.57VTLVGYY1136 pKa = 10.21QGEE1139 pKa = 4.35SGAWGII1145 pKa = 4.66

Molecular weight: 119.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I3XQH5|A0A1I3XQH5_9PROT Uncharacterized protein OS=Nitrosomonas aestuarii OX=52441 GN=SAMN05216302_100215 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.14QPSVTKK11 pKa = 10.56RR12 pKa = 11.84KK13 pKa = 7.62RR14 pKa = 11.84THH16 pKa = 5.71GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.24TRR25 pKa = 11.84GGAAVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.96GRR39 pKa = 11.84ARR41 pKa = 11.84LSVV44 pKa = 3.12

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3744

0

3744

1122864

25

3466

299.9

33.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.607 ± 0.041

1.03 ± 0.013

5.444 ± 0.031

5.972 ± 0.039

4.15 ± 0.03

6.688 ± 0.044

2.508 ± 0.023

7.014 ± 0.032

5.071 ± 0.036

10.216 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.526 ± 0.02

4.459 ± 0.036

4.165 ± 0.023

4.344 ± 0.033

5.341 ± 0.035

6.226 ± 0.034

5.519 ± 0.033

6.463 ± 0.031

1.273 ± 0.02

2.984 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski