bacterium HR12

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1868 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H5WE58|A0A2H5WE58_9BACT Purine catabolism regulatory protein OS=bacterium HR12 OX=2035407 GN=pucR_2 PE=3 SV=1
MM1 pKa = 7.59PVGAEE6 pKa = 4.1NPPGALMLMDD16 pKa = 4.64WYY18 pKa = 10.07YY19 pKa = 10.44QPKK22 pKa = 7.96IAAMVTEE29 pKa = 4.55WVLYY33 pKa = 9.59LSPCKK38 pKa = 10.15GVRR41 pKa = 11.84EE42 pKa = 4.3VILTDD47 pKa = 3.65AEE49 pKa = 4.09QALEE53 pKa = 4.37DD54 pKa = 4.57GYY56 pKa = 11.49KK57 pKa = 10.62GYY59 pKa = 11.32ANKK62 pKa = 10.5LYY64 pKa = 8.85QTAEE68 pKa = 4.08AEE70 pKa = 4.4VAFPSDD76 pKa = 3.54EE77 pKa = 4.22TLSLAEE83 pKa = 4.43FGTNITTDD91 pKa = 3.31EE92 pKa = 4.19QAQEE96 pKa = 3.37WDD98 pKa = 4.49AIFLPISQQQ107 pKa = 2.99

Molecular weight: 11.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H5WEW4|A0A2H5WEW4_9BACT Imidazolonepropionase OS=bacterium HR12 OX=2035407 GN=hutI_3 PE=4 SV=1
MM1 pKa = 7.3AVEE4 pKa = 4.17EE5 pKa = 4.05ARR7 pKa = 11.84RR8 pKa = 11.84AGVGRR13 pKa = 11.84LGGRR17 pKa = 11.84GGGLEE22 pKa = 3.95QGRR25 pKa = 11.84DD26 pKa = 3.47EE27 pKa = 4.98GPPSGRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AEE38 pKa = 3.85APQGPRR44 pKa = 11.84AGSRR48 pKa = 11.84AGAPGRR54 pKa = 11.84AARR57 pKa = 11.84AGPGAARR64 pKa = 11.84ACRR67 pKa = 11.84SSPRR71 pKa = 11.84PPTPGPTGPAAPSAPRR87 pKa = 11.84GRR89 pKa = 11.84RR90 pKa = 11.84PPAGARR96 pKa = 11.84ARR98 pKa = 11.84RR99 pKa = 11.84TRR101 pKa = 11.84RR102 pKa = 11.84GSRR105 pKa = 11.84GARR108 pKa = 11.84GAPGGSRR115 pKa = 11.84PRR117 pKa = 11.84VARR120 pKa = 11.84SAPGSGSSPRR130 pKa = 11.84RR131 pKa = 11.84GTRR134 pKa = 11.84ARR136 pKa = 11.84SVRR139 pKa = 11.84PPVRR143 pKa = 11.84SHH145 pKa = 7.09PSRR148 pKa = 11.84PTRR151 pKa = 11.84PPRR154 pKa = 11.84PQGARR159 pKa = 11.84PGPPGGSPAPGRR171 pKa = 11.84GARR174 pKa = 11.84RR175 pKa = 11.84SRR177 pKa = 11.84GSEE180 pKa = 3.92RR181 pKa = 11.84GWGRR185 pKa = 11.84PGGARR190 pKa = 11.84SPRR193 pKa = 11.84RR194 pKa = 11.84ARR196 pKa = 11.84PAPASGPRR204 pKa = 11.84GPARR208 pKa = 11.84RR209 pKa = 11.84GGTGRR214 pKa = 11.84GPPRR218 pKa = 11.84PPAPPRR224 pKa = 11.84AGGRR228 pKa = 11.84RR229 pKa = 11.84GSGAAAVGGRR239 pKa = 11.84RR240 pKa = 11.84SRR242 pKa = 11.84TTPSPGLPAGSRR254 pKa = 11.84RR255 pKa = 11.84GRR257 pKa = 11.84PARR260 pKa = 11.84RR261 pKa = 11.84AQARR265 pKa = 11.84PGPRR269 pKa = 11.84GRR271 pKa = 11.84VRR273 pKa = 11.84GAGRR277 pKa = 11.84GARR280 pKa = 11.84PRR282 pKa = 11.84RR283 pKa = 11.84WRR285 pKa = 11.84GTGRR289 pKa = 11.84GSARR293 pKa = 11.84SGGRR297 pKa = 11.84ARR299 pKa = 11.84ARR301 pKa = 11.84RR302 pKa = 11.84PARR305 pKa = 11.84AWCTRR310 pKa = 11.84SAGRR314 pKa = 11.84AGSARR319 pKa = 11.84RR320 pKa = 11.84ARR322 pKa = 11.84PAAARR327 pKa = 11.84RR328 pKa = 11.84PPRR331 pKa = 11.84RR332 pKa = 11.84RR333 pKa = 11.84GAPRR337 pKa = 11.84PRR339 pKa = 11.84RR340 pKa = 11.84GPGSRR345 pKa = 11.84VPRR348 pKa = 11.84APARR352 pKa = 11.84RR353 pKa = 11.84RR354 pKa = 11.84GLRR357 pKa = 11.84RR358 pKa = 11.84RR359 pKa = 11.84RR360 pKa = 11.84RR361 pKa = 11.84SGPRR365 pKa = 11.84APRR368 pKa = 11.84ARR370 pKa = 11.84GPRR373 pKa = 11.84PAGAGRR379 pKa = 11.84GGRR382 pKa = 11.84RR383 pKa = 11.84RR384 pKa = 11.84SAPAVPRR391 pKa = 11.84RR392 pKa = 11.84APGARR397 pKa = 11.84ARR399 pKa = 11.84ARR401 pKa = 11.84RR402 pKa = 11.84WPRR405 pKa = 11.84DD406 pKa = 3.2PGAPAGPRR414 pKa = 11.84SRR416 pKa = 11.84GARR419 pKa = 3.36

Molecular weight: 43.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1868

0

1868

496997

29

1205

266.1

28.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.115 ± 0.091

0.831 ± 0.019

5.148 ± 0.039

7.58 ± 0.066

2.942 ± 0.035

9.456 ± 0.067

1.97 ± 0.03

3.794 ± 0.051

1.572 ± 0.039

10.515 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.83 ± 0.026

1.391 ± 0.029

6.412 ± 0.059

2.039 ± 0.028

9.822 ± 0.085

4.237 ± 0.042

4.801 ± 0.045

9.136 ± 0.061

1.443 ± 0.028

1.968 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski