Amino acid dipepetide frequency for bacterium HR12

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.964AlaAla: 18.964 ± 0.287	1.304AlaCys: 1.304 ± 0.068	6.223AlaAsp: 6.223 ± 0.141	10.477AlaGlu: 10.477 ± 0.209	4.248AlaPhe: 4.248 ± 0.114	11.66AlaGly: 11.66 ± 0.182	2.25AlaHis: 2.25 ± 0.064	5.195AlaIle: 5.195 ± 0.108	2.187AlaLys: 2.187 ± 0.094	14.797AlaLeu: 14.797 ± 0.231	2.855AlaMet: 2.855 ± 0.069	1.74AlaAsn: 1.74 ± 0.058	6.604AlaPro: 6.604 ± 0.142	2.521AlaGln: 2.521 ± 0.068	12.686AlaArg: 12.686 ± 0.228	5.682AlaSer: 5.682 ± 0.135	6.06AlaThr: 6.06 ± 0.112	11.024AlaVal: 11.024 ± 0.187	2.109AlaTrp: 2.109 ± 0.067	2.561AlaTyr: 2.561 ± 0.076	0.0AlaXaa: 0.0 ± 0.0
Cys	0.956CysAla: 0.956 ± 0.046	0.093CysCys: 0.093 ± 0.015	0.499CysAsp: 0.499 ± 0.034	0.563CysGlu: 0.563 ± 0.034	0.292CysPhe: 0.292 ± 0.026	0.936CysGly: 0.936 ± 0.054	0.288CysHis: 0.288 ± 0.036	0.249CysIle: 0.249 ± 0.021	0.093CysLys: 0.093 ± 0.012	0.666CysLeu: 0.666 ± 0.037	0.119CysMet: 0.119 ± 0.015	0.123CysAsn: 0.123 ± 0.015	0.66CysPro: 0.66 ± 0.042	0.135CysGln: 0.135 ± 0.016	0.793CysArg: 0.793 ± 0.045	0.457CysSer: 0.457 ± 0.033	0.451CysThr: 0.451 ± 0.035	0.632CysVal: 0.632 ± 0.043	0.105CysTrp: 0.105 ± 0.015	0.199CysTyr: 0.199 ± 0.02	0.0CysXaa: 0.0 ± 0.0
Asp	6.342AspAla: 6.342 ± 0.109	0.35AspCys: 0.35 ± 0.025	2.525AspAsp: 2.525 ± 0.079	3.968AspGlu: 3.968 ± 0.093	1.27AspPhe: 1.27 ± 0.059	5.125AspGly: 5.125 ± 0.114	1.163AspHis: 1.163 ± 0.058	1.433AspIle: 1.433 ± 0.062	0.61AspLys: 0.61 ± 0.04	6.724AspLeu: 6.724 ± 0.129	0.642AspMet: 0.642 ± 0.039	0.507AspAsn: 0.507 ± 0.036	4.907AspPro: 4.907 ± 0.113	1.058AspGln: 1.058 ± 0.045	5.213AspArg: 5.213 ± 0.114	1.091AspSer: 1.091 ± 0.046	1.714AspThr: 1.714 ± 0.066	5.314AspVal: 5.314 ± 0.113	0.654AspTrp: 0.654 ± 0.033	0.865AspTyr: 0.865 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	10.578GluAla: 10.578 ± 0.185	0.33GluCys: 0.33 ± 0.024	3.716GluAsp: 3.716 ± 0.097	6.4GluGlu: 6.4 ± 0.142	1.286GluPhe: 1.286 ± 0.052	6.93GluGly: 6.93 ± 0.127	1.648GluHis: 1.648 ± 0.057	3.342GluIle: 3.342 ± 0.088	0.998GluLys: 0.998 ± 0.05	8.739GluLeu: 8.739 ± 0.148	1.103GluMet: 1.103 ± 0.052	0.809GluAsn: 0.809 ± 0.039	4.728GluPro: 4.728 ± 0.115	1.761GluGln: 1.761 ± 0.065	9.936GluArg: 9.936 ± 0.162	1.658GluSer: 1.658 ± 0.059	3.211GluThr: 3.211 ± 0.085	6.875GluVal: 6.875 ± 0.114	0.682GluTrp: 0.682 ± 0.038	1.068GluTyr: 1.068 ± 0.059	0.0GluXaa: 0.0 ± 0.0
Phe	3.588PheAla: 3.588 ± 0.089	0.332PheCys: 0.332 ± 0.028	1.982PheAsp: 1.982 ± 0.074	2.181PheGlu: 2.181 ± 0.077	0.976PhePhe: 0.976 ± 0.046	3.137PheGly: 3.137 ± 0.086	0.602PheHis: 0.602 ± 0.029	0.869PheIle: 0.869 ± 0.046	0.344PheLys: 0.344 ± 0.028	3.155PheLeu: 3.155 ± 0.09	0.425PheMet: 0.425 ± 0.029	0.463PheAsn: 0.463 ± 0.032	1.561PhePro: 1.561 ± 0.054	0.6PheGln: 0.6 ± 0.036	2.451PheArg: 2.451 ± 0.074	1.352PheSer: 1.352 ± 0.05	1.555PheThr: 1.555 ± 0.059	2.799PheVal: 2.799 ± 0.082	0.445PheTrp: 0.445 ± 0.027	0.606PheTyr: 0.606 ± 0.038	0.0PheXaa: 0.0 ± 0.0
Gly	10.855GlyAla: 10.855 ± 0.173	0.909GlyCys: 0.909 ± 0.046	4.561GlyAsp: 4.561 ± 0.094	6.36GlyGlu: 6.36 ± 0.129	3.167GlyPhe: 3.167 ± 0.086	8.867GlyGly: 8.867 ± 0.202	1.813GlyHis: 1.813 ± 0.068	4.085GlyIle: 4.085 ± 0.101	1.791GlyLys: 1.791 ± 0.068	9.674GlyLeu: 9.674 ± 0.166	2.356GlyMet: 2.356 ± 0.073	1.278GlyAsn: 1.278 ± 0.057	5.69GlyPro: 5.69 ± 0.133	1.785GlyGln: 1.785 ± 0.064	9.24GlyArg: 9.24 ± 0.145	4.847GlySer: 4.847 ± 0.128	5.097GlyThr: 5.097 ± 0.115	8.262GlyVal: 8.262 ± 0.15	1.672GlyTrp: 1.672 ± 0.072	2.248GlyTyr: 2.248 ± 0.07	0.0GlyXaa: 0.0 ± 0.0
His	2.366HisAla: 2.366 ± 0.07	0.155HisCys: 0.155 ± 0.018	1.107HisAsp: 1.107 ± 0.049	1.36HisGlu: 1.36 ± 0.05	0.513HisPhe: 0.513 ± 0.032	2.044HisGly: 2.044 ± 0.078	0.547HisHis: 0.547 ± 0.034	0.563HisIle: 0.563 ± 0.037	0.266HisLys: 0.266 ± 0.026	2.243HisLeu: 2.243 ± 0.077	0.278HisMet: 0.278 ± 0.026	0.239HisAsn: 0.239 ± 0.022	1.833HisPro: 1.833 ± 0.069	0.445HisGln: 0.445 ± 0.031	2.008HisArg: 2.008 ± 0.072	0.489HisSer: 0.489 ± 0.032	0.799HisThr: 0.799 ± 0.041	1.855HisVal: 1.855 ± 0.062	0.249HisTrp: 0.249 ± 0.021	0.336HisTyr: 0.336 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	5.352IleAla: 5.352 ± 0.101	0.332IleCys: 0.332 ± 0.025	2.219IleAsp: 2.219 ± 0.074	3.125IleGlu: 3.125 ± 0.077	0.928IlePhe: 0.928 ± 0.048	3.658IleGly: 3.658 ± 0.093	0.787IleHis: 0.787 ± 0.043	0.895IleIle: 0.895 ± 0.05	0.559IleLys: 0.559 ± 0.037	3.59IleLeu: 3.59 ± 0.098	0.495IleMet: 0.495 ± 0.033	0.573IleAsn: 0.573 ± 0.039	2.237IlePro: 2.237 ± 0.081	0.901IleGln: 0.901 ± 0.045	3.461IleArg: 3.461 ± 0.078	1.851IleSer: 1.851 ± 0.055	1.855IleThr: 1.855 ± 0.059	4.024IleVal: 4.024 ± 0.091	0.447IleTrp: 0.447 ± 0.027	0.646IleTyr: 0.646 ± 0.043	0.0IleXaa: 0.0 ± 0.0
Lys	1.988LysAla: 1.988 ± 0.072	0.082LysCys: 0.082 ± 0.012	0.889LysAsp: 0.889 ± 0.05	1.143LysGlu: 1.143 ± 0.053	0.286LysPhe: 0.286 ± 0.025	1.396LysGly: 1.396 ± 0.064	0.314LysHis: 0.314 ± 0.026	0.72LysIle: 0.72 ± 0.043	0.441LysLys: 0.441 ± 0.057	1.563LysLeu: 1.563 ± 0.055	0.276LysMet: 0.276 ± 0.025	0.237LysAsn: 0.237 ± 0.023	0.863LysPro: 0.863 ± 0.043	0.34LysGln: 0.34 ± 0.028	1.612LysArg: 1.612 ± 0.059	0.561LysSer: 0.561 ± 0.037	0.851LysThr: 0.851 ± 0.049	1.704LysVal: 1.704 ± 0.069	0.143LysTrp: 0.143 ± 0.019	0.31LysTyr: 0.31 ± 0.026	0.0LysXaa: 0.0 ± 0.0
Leu	16.855LeuAla: 16.855 ± 0.26	0.69LeuCys: 0.69 ± 0.036	5.606LeuAsp: 5.606 ± 0.108	8.392LeuGlu: 8.392 ± 0.125	2.833LeuPhe: 2.833 ± 0.081	10.636LeuGly: 10.636 ± 0.194	1.968LeuHis: 1.968 ± 0.073	3.215LeuIle: 3.215 ± 0.089	1.606LeuLys: 1.606 ± 0.07	10.549LeuLeu: 10.549 ± 0.185	1.459LeuMet: 1.459 ± 0.056	1.256LeuAsn: 1.256 ± 0.049	6.193LeuPro: 6.193 ± 0.116	2.119LeuGln: 2.119 ± 0.066	9.984LeuArg: 9.984 ± 0.16	4.31LeuSer: 4.31 ± 0.099	4.58LeuThr: 4.58 ± 0.097	9.952LeuVal: 9.952 ± 0.149	1.207LeuTrp: 1.207 ± 0.058	1.742LeuTyr: 1.742 ± 0.06	0.0LeuXaa: 0.0 ± 0.0
Met	2.344MetAla: 2.344 ± 0.072	0.099MetCys: 0.099 ± 0.015	0.865MetAsp: 0.865 ± 0.039	1.157MetGlu: 1.157 ± 0.056	0.419MetPhe: 0.419 ± 0.03	1.567MetGly: 1.567 ± 0.051	0.358MetHis: 0.358 ± 0.026	0.777MetIle: 0.777 ± 0.038	0.396MetLys: 0.396 ± 0.033	1.865MetLeu: 1.865 ± 0.064	0.348MetMet: 0.348 ± 0.027	0.425MetAsn: 0.425 ± 0.027	1.165MetPro: 1.165 ± 0.048	0.382MetGln: 0.382 ± 0.03	1.823MetArg: 1.823 ± 0.059	1.153MetSer: 1.153 ± 0.047	1.243MetThr: 1.243 ± 0.054	1.388MetVal: 1.388 ± 0.056	0.217MetTrp: 0.217 ± 0.022	0.304MetTyr: 0.304 ± 0.023	0.0MetXaa: 0.0 ± 0.0
Asn	1.779AsnAla: 1.779 ± 0.059	0.177AsnCys: 0.177 ± 0.022	0.658AsnAsp: 0.658 ± 0.039	0.732AsnGlu: 0.732 ± 0.042	0.461AsnPhe: 0.461 ± 0.034	1.129AsnGly: 1.129 ± 0.062	0.28AsnHis: 0.28 ± 0.024	0.563AsnIle: 0.563 ± 0.036	0.229AsnLys: 0.229 ± 0.023	1.638AsnLeu: 1.638 ± 0.062	0.219AsnMet: 0.219 ± 0.019	0.249AsnAsn: 0.249 ± 0.029	1.318AsnPro: 1.318 ± 0.053	0.288AsnGln: 0.288 ± 0.023	1.197AsnArg: 1.197 ± 0.051	0.423AsnSer: 0.423 ± 0.031	0.684AsnThr: 0.684 ± 0.033	1.356AsnVal: 1.356 ± 0.057	0.187AsnTrp: 0.187 ± 0.023	0.34AsnTyr: 0.34 ± 0.032	0.0AsnXaa: 0.0 ± 0.0
Pro	7.123ProAla: 7.123 ± 0.14	0.408ProCys: 0.408 ± 0.028	4.01ProAsp: 4.01 ± 0.104	6.29ProGlu: 6.29 ± 0.116	1.94ProPhe: 1.94 ± 0.069	6.757ProGly: 6.757 ± 0.12	1.346ProHis: 1.346 ± 0.062	2.408ProIle: 2.408 ± 0.075	0.978ProLys: 0.978 ± 0.046	5.439ProLeu: 5.439 ± 0.115	1.169ProMet: 1.169 ± 0.05	0.936ProAsn: 0.936 ± 0.046	4.608ProPro: 4.608 ± 0.12	1.256ProGln: 1.256 ± 0.062	4.954ProArg: 4.954 ± 0.127	3.443ProSer: 3.443 ± 0.101	3.394ProThr: 3.394 ± 0.085	5.366ProVal: 5.366 ± 0.087	1.006ProTrp: 1.006 ± 0.049	1.29ProTyr: 1.29 ± 0.057	0.0ProXaa: 0.0 ± 0.0
Gln	2.885GlnAla: 2.885 ± 0.082	0.135GlnCys: 0.135 ± 0.016	1.044GlnAsp: 1.044 ± 0.057	1.39GlnGlu: 1.39 ± 0.052	0.457GlnPhe: 0.457 ± 0.035	1.829GlnGly: 1.829 ± 0.065	0.406GlnHis: 0.406 ± 0.026	1.004GlnIle: 1.004 ± 0.044	0.342GlnLys: 0.342 ± 0.03	1.948GlnLeu: 1.948 ± 0.069	0.453GlnMet: 0.453 ± 0.03	0.27GlnAsn: 0.27 ± 0.025	1.28GlnPro: 1.28 ± 0.045	0.579GlnGln: 0.579 ± 0.043	2.066GlnArg: 2.066 ± 0.066	0.616GlnSer: 0.616 ± 0.04	0.891GlnThr: 0.891 ± 0.048	2.187GlnVal: 2.187 ± 0.063	0.217GlnTrp: 0.217 ± 0.021	0.394GlnTyr: 0.394 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	12.853ArgAla: 12.853 ± 0.19	0.801ArgCys: 0.801 ± 0.044	4.664ArgAsp: 4.664 ± 0.108	7.761ArgGlu: 7.761 ± 0.145	3.245ArgPhe: 3.245 ± 0.081	7.867ArgGly: 7.867 ± 0.152	1.847ArgHis: 1.847 ± 0.07	4.31ArgIle: 4.31 ± 0.096	1.543ArgLys: 1.543 ± 0.062	9.908ArgLeu: 9.908 ± 0.16	2.068ArgMet: 2.068 ± 0.06	1.245ArgAsn: 1.245 ± 0.053	6.077ArgPro: 6.077 ± 0.131	1.867ArgGln: 1.867 ± 0.055	10.795ArgArg: 10.795 ± 0.187	4.559ArgSer: 4.559 ± 0.123	4.734ArgThr: 4.734 ± 0.104	8.034ArgVal: 8.034 ± 0.112	1.684ArgTrp: 1.684 ± 0.06	2.352ArgTyr: 2.352 ± 0.081	0.0ArgXaa: 0.0 ± 0.0
Ser	4.94SerAla: 4.94 ± 0.111	0.402SerCys: 0.402 ± 0.031	1.92SerAsp: 1.92 ± 0.063	2.481SerGlu: 2.481 ± 0.062	1.569SerPhe: 1.569 ± 0.053	4.61SerGly: 4.61 ± 0.114	0.734SerHis: 0.734 ± 0.041	1.62SerIle: 1.62 ± 0.056	0.712SerLys: 0.712 ± 0.045	4.052SerLeu: 4.052 ± 0.093	1.036SerMet: 1.036 ± 0.048	0.636SerAsn: 0.636 ± 0.037	3.201SerPro: 3.201 ± 0.09	0.827SerGln: 0.827 ± 0.041	3.69SerArg: 3.69 ± 0.101	2.425SerSer: 2.425 ± 0.098	2.207SerThr: 2.207 ± 0.077	3.62SerVal: 3.62 ± 0.088	0.829SerTrp: 0.829 ± 0.041	0.859SerTyr: 0.859 ± 0.04	0.0SerXaa: 0.0 ± 0.0
Thr	5.376ThrAla: 5.376 ± 0.118	0.525ThrCys: 0.525 ± 0.042	2.31ThrAsp: 2.31 ± 0.076	2.946ThrGlu: 2.946 ± 0.074	1.843ThrPhe: 1.843 ± 0.07	5.095ThrGly: 5.095 ± 0.107	0.881ThrHis: 0.881 ± 0.043	2.187ThrIle: 2.187 ± 0.071	0.803ThrLys: 0.803 ± 0.045	5.012ThrLeu: 5.012 ± 0.112	0.994ThrMet: 0.994 ± 0.047	0.849ThrAsn: 0.849 ± 0.046	3.447ThrPro: 3.447 ± 0.086	0.934ThrGln: 0.934 ± 0.043	3.557ThrArg: 3.557 ± 0.1	2.304ThrSer: 2.304 ± 0.086	2.547ThrThr: 2.547 ± 0.079	4.487ThrVal: 4.487 ± 0.105	0.801ThrTrp: 0.801 ± 0.045	1.117ThrTyr: 1.117 ± 0.049	0.0ThrXaa: 0.0 ± 0.0
Val	12.046ValAla: 12.046 ± 0.184	0.857ValCys: 0.857 ± 0.054	4.765ValAsp: 4.765 ± 0.103	6.841ValGlu: 6.841 ± 0.132	2.535ValPhe: 2.535 ± 0.072	8.262ValGly: 8.262 ± 0.143	1.742ValHis: 1.742 ± 0.069	3.38ValIle: 3.38 ± 0.088	1.304ValLys: 1.304 ± 0.052	9.624ValLeu: 9.624 ± 0.156	1.443ValMet: 1.443 ± 0.058	1.499ValAsn: 1.499 ± 0.063	5.811ValPro: 5.811 ± 0.111	1.775ValGln: 1.775 ± 0.06	9.135ValArg: 9.135 ± 0.147	3.688ValSer: 3.688 ± 0.092	4.549ValThr: 4.549 ± 0.09	9.292ValVal: 9.292 ± 0.158	1.171ValTrp: 1.171 ± 0.059	1.642ValTyr: 1.642 ± 0.058	0.0ValXaa: 0.0 ± 0.0
Trp	1.642TrpAla: 1.642 ± 0.059	0.163TrpCys: 0.163 ± 0.019	0.726TrpAsp: 0.726 ± 0.04	0.779TrpGlu: 0.779 ± 0.043	0.579TrpPhe: 0.579 ± 0.036	1.113TrpGly: 1.113 ± 0.044	0.274TrpHis: 0.274 ± 0.022	0.648TrpIle: 0.648 ± 0.032	0.26TrpLys: 0.26 ± 0.023	1.561TrpLeu: 1.561 ± 0.063	0.32TrpMet: 0.32 ± 0.025	0.268TrpAsn: 0.268 ± 0.022	0.779TrpPro: 0.779 ± 0.044	0.342TrpGln: 0.342 ± 0.026	1.439TrpArg: 1.439 ± 0.056	0.789TrpSer: 0.789 ± 0.04	0.763TrpThr: 0.763 ± 0.035	1.292TrpVal: 1.292 ± 0.054	0.348TrpTrp: 0.348 ± 0.025	0.348TrpTyr: 0.348 ± 0.026	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.316TyrAla: 2.316 ± 0.069	0.161TyrCys: 0.161 ± 0.018	1.185TyrAsp: 1.185 ± 0.051	1.451TyrGlu: 1.451 ± 0.062	0.575TyrPhe: 0.575 ± 0.034	1.94TyrGly: 1.94 ± 0.052	0.449TyrHis: 0.449 ± 0.033	0.473TyrIle: 0.473 ± 0.032	0.26TyrLys: 0.26 ± 0.025	2.404TyrLeu: 2.404 ± 0.073	0.237TyrMet: 0.237 ± 0.022	0.306TyrAsn: 0.306 ± 0.025	1.157TyrPro: 1.157 ± 0.056	0.481TyrGln: 0.481 ± 0.032	2.177TyrArg: 2.177 ± 0.08	0.674TyrSer: 0.674 ± 0.036	0.827TyrThr: 0.827 ± 0.044	1.897TyrVal: 1.897 ± 0.066	0.258TyrTrp: 0.258 ± 0.022	0.455TyrTyr: 0.455 ± 0.03	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 1868 proteins (496997 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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