Amphritea balenae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Oceanospirillaceae; Amphritea

Average proteome isoelectric point is 5.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4082 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3P1SIE3|A0A3P1SIE3_9GAMM Thiosulfate reductase OS=Amphritea balenae OX=452629 GN=EHS89_19010 PE=4 SV=1
MM1 pKa = 7.47RR2 pKa = 11.84TFSSFFKK9 pKa = 9.94LTSFIMGSVFAAALVIMMTVAAVDD33 pKa = 3.51SLGVFEE39 pKa = 6.03LDD41 pKa = 4.67GDD43 pKa = 4.18ATDD46 pKa = 4.33LASGTGSAGDD56 pKa = 4.15DD57 pKa = 3.3WSSLYY62 pKa = 10.06IDD64 pKa = 4.2CPEE67 pKa = 4.09GNVRR71 pKa = 11.84DD72 pKa = 4.29NPPGDD77 pKa = 4.05DD78 pKa = 4.33CPPGLSSVFTGIINDD93 pKa = 3.97EE94 pKa = 4.29NPSPVDD100 pKa = 3.45GTPDD104 pKa = 3.69SSIFWKK110 pKa = 10.67GGSKK114 pKa = 10.35DD115 pKa = 3.3IHH117 pKa = 7.07DD118 pKa = 4.3VPDD121 pKa = 2.71WWFKK125 pKa = 11.18AGSTPDD131 pKa = 3.36KK132 pKa = 11.1DD133 pKa = 4.08QITNAYY139 pKa = 8.37AAAYY143 pKa = 10.14SLTSGNGIHH152 pKa = 6.04QVGDD156 pKa = 3.96LIVNFGLDD164 pKa = 2.9RR165 pKa = 11.84FANNGDD171 pKa = 3.06AHH173 pKa = 6.67AGFWFFHH180 pKa = 6.34QDD182 pKa = 3.19VGPDD186 pKa = 3.33PSRR189 pKa = 11.84PSRR192 pKa = 11.84FTGMHH197 pKa = 4.2QQKK200 pKa = 10.26NISIGQRR207 pKa = 11.84GDD209 pKa = 3.03ILVLVEE215 pKa = 4.3YY216 pKa = 9.59PQGSNANPLIRR227 pKa = 11.84VYY229 pKa = 10.48EE230 pKa = 4.12WDD232 pKa = 4.27PLDD235 pKa = 4.88EE236 pKa = 6.22GDD238 pKa = 5.98DD239 pKa = 3.67NAAKK243 pKa = 10.28NLHH246 pKa = 5.56QLYY249 pKa = 10.8DD250 pKa = 3.84SDD252 pKa = 4.3TSGQSATCGVGSGGNKK268 pKa = 9.08LACAITNDD276 pKa = 3.61GSVNAPWSYY285 pKa = 10.12TPKK288 pKa = 10.21SGPGGSFPAEE298 pKa = 3.88SFYY301 pKa = 11.23EE302 pKa = 4.17GSINFTQLLGMTPCFSSFLAEE323 pKa = 3.97TRR325 pKa = 11.84ASRR328 pKa = 11.84SEE330 pKa = 3.92DD331 pKa = 3.05AKK333 pKa = 11.2LWDD336 pKa = 3.69HH337 pKa = 6.92VGGEE341 pKa = 4.52FDD343 pKa = 3.73LCSIDD348 pKa = 3.7VLKK351 pKa = 10.33TGDD354 pKa = 3.82TLSKK358 pKa = 9.97VTDD361 pKa = 3.72PVDD364 pKa = 3.41YY365 pKa = 9.93TFKK368 pKa = 9.84ITNTGAITLYY378 pKa = 10.26QDD380 pKa = 4.81FIIDD384 pKa = 3.81TLLGDD389 pKa = 4.54LVDD392 pKa = 4.59PVPASHH398 pKa = 7.19LYY400 pKa = 8.28VTSSDD405 pKa = 4.25CGPQGAALLAGHH417 pKa = 6.63YY418 pKa = 7.44CTIEE422 pKa = 3.59ATRR425 pKa = 11.84IVVGDD430 pKa = 4.05EE431 pKa = 3.6ADD433 pKa = 4.22GIYY436 pKa = 10.58DD437 pKa = 3.33SSGVLQILPNTLNVSYY453 pKa = 11.18NNQSDD458 pKa = 4.08LAGDD462 pKa = 4.09SVDD465 pKa = 4.18GSDD468 pKa = 3.71DD469 pKa = 3.47HH470 pKa = 7.94SVNLFQPSFTLVKK483 pKa = 10.0SCPIGIGSGDD493 pKa = 3.86GEE495 pKa = 4.42RR496 pKa = 11.84AWKK499 pKa = 10.39VGEE502 pKa = 4.01PLDD505 pKa = 4.78FRR507 pKa = 11.84FTLTNTSSSDD517 pKa = 3.52TPIMEE522 pKa = 5.2LGSLTDD528 pKa = 4.12PLFGGNILALLPQAKK543 pKa = 9.51QNALAEE549 pKa = 4.14LAIDD553 pKa = 3.73EE554 pKa = 5.27SIDD557 pKa = 3.58FVLSYY562 pKa = 10.39TPSSNDD568 pKa = 2.79IPGVTNTADD577 pKa = 3.25AVYY580 pKa = 10.36SPQGFTNQIAANNGTPASVFCPVALPGPATILIEE614 pKa = 4.18KK615 pKa = 8.67EE616 pKa = 3.74LRR618 pKa = 11.84GGGGVTFTFTGGGLLVPGLTPVIGGNTTEE647 pKa = 4.37SPHH650 pKa = 6.02GAVRR654 pKa = 11.84DD655 pKa = 3.82AAVDD659 pKa = 4.84GYY661 pKa = 11.34AVTGSISVSTTFGTSTQYY679 pKa = 10.74TVTEE683 pKa = 4.23SGLPAGTTFEE693 pKa = 4.6SVSCRR698 pKa = 11.84NPDD701 pKa = 3.37GEE703 pKa = 5.49LDD705 pKa = 3.75TDD707 pKa = 4.36SSASGATATINVDD720 pKa = 3.15EE721 pKa = 5.3LEE723 pKa = 4.41VVVCRR728 pKa = 11.84FINIVEE734 pKa = 4.25PKK736 pKa = 8.5LTLVKK741 pKa = 10.59SVTNDD746 pKa = 2.93NGGNAVPGDD755 pKa = 3.94FEE757 pKa = 5.26LVLNGGIYY765 pKa = 10.07SDD767 pKa = 3.99TVFSDD772 pKa = 3.34GAMPTVVAEE781 pKa = 4.05TAYY784 pKa = 9.81TLTEE788 pKa = 4.12NPFPGYY794 pKa = 10.36SQDD797 pKa = 3.51GDD799 pKa = 4.05VACVDD804 pKa = 4.35DD805 pKa = 4.47NTAQTVSHH813 pKa = 7.13PVTLDD818 pKa = 3.38FGQEE822 pKa = 3.99VTCTVVNDD830 pKa = 5.38DD831 pKa = 3.57ITPTITLIKK840 pKa = 10.35EE841 pKa = 4.21VVNDD845 pKa = 3.45NGGNAGVNDD854 pKa = 4.77FGLSIGGTGVNSGVTLDD871 pKa = 3.39VDD873 pKa = 4.04ANIAIALTEE882 pKa = 4.38AGLTGYY888 pKa = 10.0EE889 pKa = 4.36FVSIGQGGGDD899 pKa = 3.48AAKK902 pKa = 10.55CPAVLNGTVTLDD914 pKa = 3.38EE915 pKa = 5.43DD916 pKa = 3.72EE917 pKa = 6.14HH918 pKa = 5.69ITCTIVNDD926 pKa = 5.11DD927 pKa = 3.85IAPSITLIKK936 pKa = 10.35EE937 pKa = 4.16VINDD941 pKa = 3.41NGGNAGVNDD950 pKa = 4.77FGLSIGGTGVNSGEE964 pKa = 4.28TLDD967 pKa = 4.36VDD969 pKa = 4.22ANTAIALTEE978 pKa = 4.36AGLTGYY984 pKa = 10.43NFVSIGQGGGDD995 pKa = 3.6SAKK998 pKa = 10.59CPAVLNGTVTLDD1010 pKa = 3.32EE1011 pKa = 5.3DD1012 pKa = 3.86EE1013 pKa = 5.64HH1014 pKa = 5.28VTCTIVNDD1022 pKa = 5.23DD1023 pKa = 3.71ITPTITLIKK1032 pKa = 10.29EE1033 pKa = 4.23VINDD1037 pKa = 3.41NGGNAGVNDD1046 pKa = 4.77FGLSIGGTGVNSGLTLDD1063 pKa = 4.1VDD1065 pKa = 4.31ANTAIALTEE1074 pKa = 4.36AGLTGYY1080 pKa = 10.43NFVSIGQGNGDD1091 pKa = 3.83SAKK1094 pKa = 10.58CPAVLNGTVTLNEE1107 pKa = 4.7DD1108 pKa = 3.41EE1109 pKa = 5.59HH1110 pKa = 5.5VTCTIVNDD1118 pKa = 5.23DD1119 pKa = 3.71ITPTITLIKK1128 pKa = 10.39HH1129 pKa = 6.06VINDD1133 pKa = 3.45NGGNAGVNDD1142 pKa = 4.77FGLSIGGAGVNSGVTLDD1159 pKa = 3.62VEE1161 pKa = 4.55ANIAIALTEE1170 pKa = 4.06VGLTGYY1176 pKa = 9.91EE1177 pKa = 4.14FVSIGQGNNDD1187 pKa = 3.57SAKK1190 pKa = 10.53CPAVLNGTVTLDD1202 pKa = 3.32EE1203 pKa = 5.3DD1204 pKa = 3.86EE1205 pKa = 5.64HH1206 pKa = 5.28VTCTIVNDD1214 pKa = 5.23DD1215 pKa = 3.71ITPTITLIKK1224 pKa = 10.29EE1225 pKa = 4.23VINDD1229 pKa = 3.41NGGNAGVNDD1238 pKa = 4.77FGLSIGGAGVNSGLTLDD1255 pKa = 4.1VDD1257 pKa = 4.31ANTAIALTEE1266 pKa = 4.36AGLTGYY1272 pKa = 10.43NFVSIGQGNGDD1283 pKa = 3.83SAKK1286 pKa = 10.58CPAVLNGTVTLDD1298 pKa = 3.32EE1299 pKa = 5.3DD1300 pKa = 3.86EE1301 pKa = 5.64HH1302 pKa = 5.28VTCTIVNDD1310 pKa = 5.23DD1311 pKa = 3.71ITPTITLIKK1320 pKa = 10.39HH1321 pKa = 6.06VINDD1325 pKa = 3.45NGGNAGVNDD1334 pKa = 4.77FGLSIGGAGVNSGVTLDD1351 pKa = 3.39VDD1353 pKa = 4.04ANIAIALTEE1362 pKa = 4.38AGLTGYY1368 pKa = 10.0EE1369 pKa = 4.36FVSIGQGNNDD1379 pKa = 3.57SAKK1382 pKa = 10.53CPAVLNGTVTLNEE1395 pKa = 4.7DD1396 pKa = 3.41EE1397 pKa = 5.59HH1398 pKa = 5.5VTCTIVNDD1406 pKa = 5.23DD1407 pKa = 3.71ITPTITLIKK1416 pKa = 10.27EE1417 pKa = 4.24VINDD1421 pKa = 3.8DD1422 pKa = 3.77GGTAGVNDD1430 pKa = 4.82FGLSIGGTGVNSGVTLDD1447 pKa = 3.72VDD1449 pKa = 4.08ANTAIALNEE1458 pKa = 4.27AGLMGYY1464 pKa = 9.37EE1465 pKa = 4.4FVSIGQGNGDD1475 pKa = 3.83SAKK1478 pKa = 10.58CPAVLNGTVTLNEE1491 pKa = 4.7DD1492 pKa = 3.41EE1493 pKa = 5.59HH1494 pKa = 5.5VTCTIVNDD1502 pKa = 3.92DD1503 pKa = 4.82LLVEE1507 pKa = 4.91FCPEE1511 pKa = 4.11PGVSGRR1517 pKa = 11.84PKK1519 pKa = 10.11EE1520 pKa = 4.04LTMMYY1525 pKa = 10.33DD1526 pKa = 3.65GVSDD1530 pKa = 3.47RR1531 pKa = 11.84TGPQTGNEE1539 pKa = 4.12VVIDD1543 pKa = 4.09PFNVVAPDD1551 pKa = 3.42EE1552 pKa = 4.76VYY1554 pKa = 10.96VEE1556 pKa = 4.82VYY1558 pKa = 6.67THH1560 pKa = 6.9RR1561 pKa = 11.84GKK1563 pKa = 10.93LVLTTGALEE1572 pKa = 4.45EE1573 pKa = 4.78GDD1575 pKa = 3.87SFSYY1579 pKa = 10.85AGFKK1583 pKa = 10.79GGVSPRR1589 pKa = 11.84SRR1591 pKa = 11.84FLIYY1595 pKa = 10.37DD1596 pKa = 3.68YY1597 pKa = 10.95ATGDD1601 pKa = 4.62LIQTVQFHH1609 pKa = 6.18TSCSQPINLGDD1620 pKa = 3.74SYY1622 pKa = 12.14GLLTVSGGILGKK1634 pKa = 10.46

Molecular weight: 168.74 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3P1SQJ5|A0A3P1SQJ5_9GAMM Glycolate oxidase subunit GlcE OS=Amphritea balenae OX=452629 GN=glcE PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLKK11 pKa = 10.2RR12 pKa = 11.84KK13 pKa = 7.57RR14 pKa = 11.84THH16 pKa = 5.9GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPSGRR28 pKa = 11.84ALINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.81GRR39 pKa = 11.84KK40 pKa = 8.77RR41 pKa = 11.84LAAA44 pKa = 4.42

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4082

0

4082

1376603

21

14333

337.2

37.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.866 ± 0.046

1.06 ± 0.021

5.896 ± 0.089

6.269 ± 0.051

3.867 ± 0.029

7.343 ± 0.068

2.074 ± 0.029

6.263 ± 0.031

4.429 ± 0.051

10.727 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.607 ± 0.032

3.953 ± 0.038

4.103 ± 0.03

4.808 ± 0.053

4.94 ± 0.071

6.624 ± 0.041

5.326 ± 0.117

6.783 ± 0.045

1.213 ± 0.015

2.849 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski