Temperate phage phiNIH1.1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q938L8|Q938L8_9CAUD Uncharacterized protein OS=Temperate phage phiNIH1.1 OX=173707 PE=4 SV=1
MM1 pKa = 7.43GFVDD5 pKa = 4.76KK6 pKa = 10.32KK7 pKa = 10.74LSEE10 pKa = 3.74ITPYY14 pKa = 11.02KK15 pKa = 10.45NNPRR19 pKa = 11.84NNDD22 pKa = 3.28EE23 pKa = 4.16AVGPVAEE30 pKa = 4.73SIKK33 pKa = 10.64EE34 pKa = 3.9FGFKK38 pKa = 10.7VPIVVDD44 pKa = 3.73KK45 pKa = 11.17NGEE48 pKa = 4.09IINGHH53 pKa = 5.0TRR55 pKa = 11.84YY56 pKa = 9.95KK57 pKa = 10.49AAQKK61 pKa = 10.59LGLEE65 pKa = 4.26TVPVIVADD73 pKa = 4.4DD74 pKa = 3.97LSEE77 pKa = 4.06EE78 pKa = 4.08QIKK81 pKa = 10.65AFRR84 pKa = 11.84LADD87 pKa = 3.58NKK89 pKa = 10.41VGEE92 pKa = 4.2IAVWDD97 pKa = 4.04LDD99 pKa = 4.36LLNEE103 pKa = 4.26EE104 pKa = 5.17LNDD107 pKa = 4.3ILDD110 pKa = 4.45LDD112 pKa = 3.76MSAFGFEE119 pKa = 5.36LEE121 pKa = 4.98IDD123 pKa = 4.55DD124 pKa = 5.0EE125 pKa = 4.41NQEE128 pKa = 4.06NLDD131 pKa = 3.56ADD133 pKa = 3.88FEE135 pKa = 4.69EE136 pKa = 6.03IEE138 pKa = 4.73DD139 pKa = 3.95DD140 pKa = 3.71SVLIVEE146 pKa = 4.79AEE148 pKa = 4.12SEE150 pKa = 4.27EE151 pKa = 4.18EE152 pKa = 4.32LEE154 pKa = 4.0KK155 pKa = 11.04LYY157 pKa = 11.23DD158 pKa = 3.62EE159 pKa = 4.51FVEE162 pKa = 5.0RR163 pKa = 11.84GFKK166 pKa = 10.58CRR168 pKa = 11.84VSILL172 pKa = 3.46

Molecular weight: 19.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q938I9|Q938I9_9CAUD Uncharacterized protein OS=Temperate phage phiNIH1.1 OX=173707 PE=4 SV=1
MM1 pKa = 7.38AKK3 pKa = 10.08VVVEE7 pKa = 3.73LGGIKK12 pKa = 10.21RR13 pKa = 11.84KK14 pKa = 10.05VSPQALAKK22 pKa = 10.53GKK24 pKa = 10.57LIMNNQVMMSMNPYY38 pKa = 9.39VPYY41 pKa = 10.15RR42 pKa = 11.84DD43 pKa = 3.27GALRR47 pKa = 11.84GSSRR51 pKa = 11.84ANSVGVTWSGPHH63 pKa = 6.37ARR65 pKa = 11.84AQFYY69 pKa = 10.53GGAYY73 pKa = 9.93NKK75 pKa = 10.37YY76 pKa = 10.46KK77 pKa = 10.58SFKK80 pKa = 8.64FKK82 pKa = 10.8KK83 pKa = 8.45YY84 pKa = 5.33TTPGTGKK91 pKa = 10.19RR92 pKa = 11.84WDD94 pKa = 3.49KK95 pKa = 10.88RR96 pKa = 11.84ALANATIVKK105 pKa = 9.88DD106 pKa = 3.5WEE108 pKa = 4.16KK109 pKa = 11.26SLLRR113 pKa = 11.84GMGFKK118 pKa = 10.45

Molecular weight: 13.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

11422

49

1086

207.7

23.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.284 ± 0.657

0.683 ± 0.121

7.039 ± 0.397

7.416 ± 0.376

3.878 ± 0.265

5.901 ± 0.297

1.471 ± 0.142

7.03 ± 0.239

9.009 ± 0.365

8.274 ± 0.241

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.268 ± 0.152

5.665 ± 0.257

2.627 ± 0.202

3.905 ± 0.273

4.097 ± 0.262

5.971 ± 0.334

6.155 ± 0.282

6.417 ± 0.24

1.121 ± 0.129

3.791 ± 0.305
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski