Sewage-associated gemycircularvirus 4

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemykrogvirus; Gemykrogvirus sewopo1; Sewage derived gemykrogvirus 1

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7CL85|A0A0A7CL85_9VIRU RepA OS=Sewage-associated gemycircularvirus 4 OX=1519399 PE=3 SV=1
MM1 pKa = 7.68PRR3 pKa = 11.84FQARR7 pKa = 11.84AKK9 pKa = 10.39AFIVTFPQVSEE20 pKa = 4.81DD21 pKa = 3.29VQQRR25 pKa = 11.84FDD27 pKa = 3.79HH28 pKa = 6.59EE29 pKa = 4.66GASLLLNITQDD40 pKa = 4.22LKK42 pKa = 11.42DD43 pKa = 3.81PSCFRR48 pKa = 11.84LGRR51 pKa = 11.84EE52 pKa = 3.66RR53 pKa = 11.84HH54 pKa = 4.95QDD56 pKa = 3.28GGVHH60 pKa = 4.27YY61 pKa = 10.47HH62 pKa = 6.16MYY64 pKa = 10.16IGFDD68 pKa = 3.29EE69 pKa = 4.52VVHH72 pKa = 6.45INRR75 pKa = 11.84ANLFDD80 pKa = 3.8YY81 pKa = 10.9FGAHH85 pKa = 6.22GNIKK89 pKa = 9.47SVRR92 pKa = 11.84RR93 pKa = 11.84TPRR96 pKa = 11.84TVYY99 pKa = 10.19DD100 pKa = 3.55YY101 pKa = 10.92CGKK104 pKa = 10.53DD105 pKa = 2.73GDD107 pKa = 3.92VRR109 pKa = 11.84YY110 pKa = 10.08EE111 pKa = 3.96RR112 pKa = 11.84GDD114 pKa = 3.43PPEE117 pKa = 4.17SVSRR121 pKa = 11.84SRR123 pKa = 11.84GEE125 pKa = 4.33NGEE128 pKa = 4.1KK129 pKa = 9.32WHH131 pKa = 6.76TICDD135 pKa = 3.92APDD138 pKa = 3.8KK139 pKa = 10.79DD140 pKa = 4.34TFLSLCRR147 pKa = 11.84QLAPKK152 pKa = 10.2DD153 pKa = 3.39WLLSNSRR160 pKa = 11.84ILEE163 pKa = 4.18YY164 pKa = 11.17ANTYY168 pKa = 9.26YY169 pKa = 10.32PEE171 pKa = 4.74IPSPYY176 pKa = 9.02EE177 pKa = 3.76GPPIIAEE184 pKa = 3.92MEE186 pKa = 4.17RR187 pKa = 11.84YY188 pKa = 9.5PEE190 pKa = 4.05LEE192 pKa = 3.7RR193 pKa = 11.84WLEE196 pKa = 3.73QARR199 pKa = 11.84IGDD202 pKa = 4.02DD203 pKa = 3.28RR204 pKa = 11.84PEE206 pKa = 3.72RR207 pKa = 4.64

Molecular weight: 24.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7CL63|A0A0A7CL63_9VIRU Replication-associated protein OS=Sewage-associated gemycircularvirus 4 OX=1519399 PE=3 SV=1
MM1 pKa = 7.37ARR3 pKa = 11.84SFKK6 pKa = 9.99PRR8 pKa = 11.84YY9 pKa = 8.83RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84FSTRR16 pKa = 11.84RR17 pKa = 11.84PSFRR21 pKa = 11.84RR22 pKa = 11.84KK23 pKa = 7.01TYY25 pKa = 9.59RR26 pKa = 11.84GRR28 pKa = 11.84RR29 pKa = 11.84YY30 pKa = 9.6ARR32 pKa = 11.84RR33 pKa = 11.84PKK35 pKa = 10.15RR36 pKa = 11.84SRR38 pKa = 11.84VTPRR42 pKa = 11.84KK43 pKa = 9.3VRR45 pKa = 11.84NIASKK50 pKa = 10.55KK51 pKa = 9.86KK52 pKa = 9.85QDD54 pKa = 3.54TLLGSDD60 pKa = 4.39SSDD63 pKa = 3.26PDD65 pKa = 3.63DD66 pKa = 4.45FSNVVALTTGNNYY79 pKa = 9.53FLWTPTFRR87 pKa = 11.84DD88 pKa = 3.15RR89 pKa = 11.84DD90 pKa = 4.11DD91 pKa = 3.69NVNNHH96 pKa = 4.88TRR98 pKa = 11.84GAASCYY104 pKa = 9.12YY105 pKa = 9.99RR106 pKa = 11.84GVRR109 pKa = 11.84DD110 pKa = 4.25RR111 pKa = 11.84IMVAASFQLIHH122 pKa = 6.85RR123 pKa = 11.84RR124 pKa = 11.84VCFWSHH130 pKa = 4.88SQISSATPYY139 pKa = 10.25DD140 pKa = 3.45IPGDD144 pKa = 4.25DD145 pKa = 4.24GDD147 pKa = 3.31IAYY150 pKa = 7.77TRR152 pKa = 11.84RR153 pKa = 11.84PLQKK157 pKa = 9.91INPSTDD163 pKa = 2.97TATFRR168 pKa = 11.84YY169 pKa = 9.32LFKK172 pKa = 10.6GTVGVDD178 pKa = 3.75FTEE181 pKa = 4.58DD182 pKa = 3.75SRR184 pKa = 11.84WDD186 pKa = 3.36SPMANNRR193 pKa = 11.84IQVVYY198 pKa = 10.6DD199 pKa = 3.1RR200 pKa = 11.84QYY202 pKa = 11.26PVNPNYY208 pKa = 10.55NVAEE212 pKa = 4.33GNAFGKK218 pKa = 10.44ISTKK222 pKa = 10.66KK223 pKa = 9.61LWHH226 pKa = 6.61PMNSTVKK233 pKa = 10.58YY234 pKa = 10.47DD235 pKa = 3.15DD236 pKa = 3.64WEE238 pKa = 3.98QGRR241 pKa = 11.84EE242 pKa = 3.44IDD244 pKa = 3.72AASWGNAHH252 pKa = 6.96PASRR256 pKa = 11.84GNFYY260 pKa = 10.57ILDD263 pKa = 3.9MFSTGQDD270 pKa = 3.45VPSEE274 pKa = 4.29SATQCGSYY282 pKa = 8.87STEE285 pKa = 3.78STVYY289 pKa = 8.67WHH291 pKa = 6.9EE292 pKa = 4.22SSS294 pKa = 3.93

Molecular weight: 34.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

831

207

330

277.0

32.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.415 ± 0.534

1.685 ± 0.328

7.702 ± 0.217

5.656 ± 1.658

4.934 ± 0.259

6.619 ± 0.597

2.768 ± 0.372

4.934 ± 0.59

4.573 ± 0.47

5.897 ± 1.404

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.564 ± 0.07

4.452 ± 0.676

6.017 ± 0.455

3.49 ± 0.221

9.627 ± 1.084

7.1 ± 1.534

4.934 ± 1.263

5.295 ± 0.256

2.046 ± 0.232

5.295 ± 0.079
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski