Amino acid dipepetide frequency for Clostridium sulfidigenes

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.748AlaAla: 3.748 ± 0.09	0.74AlaCys: 0.74 ± 0.028	2.406AlaAsp: 2.406 ± 0.057	3.286AlaGlu: 3.286 ± 0.071	2.428AlaPhe: 2.428 ± 0.057	3.637AlaGly: 3.637 ± 0.076	0.841AlaHis: 0.841 ± 0.032	6.138AlaIle: 6.138 ± 0.103	4.419AlaLys: 4.419 ± 0.071	5.989AlaLeu: 5.989 ± 0.093	1.844AlaMet: 1.844 ± 0.046	2.5AlaAsn: 2.5 ± 0.048	1.368AlaPro: 1.368 ± 0.045	1.401AlaGln: 1.401 ± 0.039	1.851AlaArg: 1.851 ± 0.046	3.229AlaSer: 3.229 ± 0.061	3.041AlaThr: 3.041 ± 0.067	3.72AlaVal: 3.72 ± 0.082	0.371AlaTrp: 0.371 ± 0.023	2.093AlaTyr: 2.093 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	0.619CysAla: 0.619 ± 0.024	0.198CysCys: 0.198 ± 0.014	0.816CysAsp: 0.816 ± 0.03	0.893CysGlu: 0.893 ± 0.03	0.515CysPhe: 0.515 ± 0.024	1.192CysGly: 1.192 ± 0.043	0.262CysHis: 0.262 ± 0.016	1.281CysIle: 1.281 ± 0.033	1.139CysLys: 1.139 ± 0.037	0.85CysLeu: 0.85 ± 0.029	0.271CysMet: 0.271 ± 0.02	0.877CysAsn: 0.877 ± 0.033	0.538CysPro: 0.538 ± 0.032	0.23CysGln: 0.23 ± 0.015	0.441CysArg: 0.441 ± 0.02	0.861CysSer: 0.861 ± 0.034	0.649CysThr: 0.649 ± 0.028	0.708CysVal: 0.708 ± 0.029	0.075CysTrp: 0.075 ± 0.01	0.482CysTyr: 0.482 ± 0.023	0.0CysXaa: 0.0 ± 0.0
Asp	2.523AspAla: 2.523 ± 0.061	0.625AspCys: 0.625 ± 0.027	2.674AspAsp: 2.674 ± 0.063	4.822AspGlu: 4.822 ± 0.077	2.767AspPhe: 2.767 ± 0.06	3.36AspGly: 3.36 ± 0.065	0.651AspHis: 0.651 ± 0.029	6.383AspIle: 6.383 ± 0.096	5.278AspLys: 5.278 ± 0.085	4.744AspLeu: 4.744 ± 0.075	1.658AspMet: 1.658 ± 0.04	3.277AspAsn: 3.277 ± 0.062	1.158AspPro: 1.158 ± 0.041	0.699AspGln: 0.699 ± 0.027	1.857AspArg: 1.857 ± 0.04	3.103AspSer: 3.103 ± 0.058	2.556AspThr: 2.556 ± 0.053	3.567AspVal: 3.567 ± 0.066	0.334AspTrp: 0.334 ± 0.017	2.548AspTyr: 2.548 ± 0.055	0.0AspXaa: 0.0 ± 0.0
Glu	4.267GluAla: 4.267 ± 0.082	0.732GluCys: 0.732 ± 0.027	4.683GluAsp: 4.683 ± 0.076	7.941GluGlu: 7.941 ± 0.122	3.248GluPhe: 3.248 ± 0.063	4.542GluGly: 4.542 ± 0.078	0.926GluHis: 0.926 ± 0.032	7.259GluIle: 7.259 ± 0.091	7.3GluLys: 7.3 ± 0.093	7.034GluLeu: 7.034 ± 0.101	1.918GluMet: 1.918 ± 0.046	5.325GluAsn: 5.325 ± 0.084	1.466GluPro: 1.466 ± 0.04	1.632GluGln: 1.632 ± 0.05	2.671GluArg: 2.671 ± 0.061	3.817GluSer: 3.817 ± 0.062	2.9GluThr: 2.9 ± 0.053	5.428GluVal: 5.428 ± 0.084	0.383GluTrp: 0.383 ± 0.021	3.308GluTyr: 3.308 ± 0.063	0.0GluXaa: 0.0 ± 0.0
Phe	2.18PheAla: 2.18 ± 0.05	0.556PheCys: 0.556 ± 0.026	2.327PheAsp: 2.327 ± 0.056	2.637PheGlu: 2.637 ± 0.062	1.876PhePhe: 1.876 ± 0.049	2.862PheGly: 2.862 ± 0.069	0.649PheHis: 0.649 ± 0.026	4.908PheIle: 4.908 ± 0.1	3.868PheLys: 3.868 ± 0.065	3.859PheLeu: 3.859 ± 0.078	1.244PheMet: 1.244 ± 0.039	2.969PheAsn: 2.969 ± 0.064	1.201PhePro: 1.201 ± 0.039	1.048PheGln: 1.048 ± 0.04	1.259PheArg: 1.259 ± 0.043	3.077PheSer: 3.077 ± 0.062	2.383PheThr: 2.383 ± 0.054	2.567PheVal: 2.567 ± 0.063	0.331PheTrp: 0.331 ± 0.022	1.821PheTyr: 1.821 ± 0.052	0.0PheXaa: 0.0 ± 0.0
Gly	4.211GlyAla: 4.211 ± 0.086	1.024GlyCys: 1.024 ± 0.037	3.375GlyAsp: 3.375 ± 0.066	4.611GlyGlu: 4.611 ± 0.072	3.164GlyPhe: 3.164 ± 0.064	4.383GlyGly: 4.383 ± 0.085	1.017GlyHis: 1.017 ± 0.033	7.185GlyIle: 7.185 ± 0.089	5.518GlyLys: 5.518 ± 0.086	5.402GlyLeu: 5.402 ± 0.087	1.863GlyMet: 1.863 ± 0.048	3.253GlyAsn: 3.253 ± 0.063	1.25GlyPro: 1.25 ± 0.041	1.474GlyGln: 1.474 ± 0.041	2.375GlyArg: 2.375 ± 0.054	3.647GlySer: 3.647 ± 0.068	3.517GlyThr: 3.517 ± 0.075	4.841GlyVal: 4.841 ± 0.089	0.481GlyTrp: 0.481 ± 0.023	3.076GlyTyr: 3.076 ± 0.061	0.0GlyXaa: 0.0 ± 0.0
His	0.638HisAla: 0.638 ± 0.025	0.248HisCys: 0.248 ± 0.017	0.737HisAsp: 0.737 ± 0.026	0.93HisGlu: 0.93 ± 0.034	0.604HisPhe: 0.604 ± 0.025	1.132HisGly: 1.132 ± 0.038	0.314HisHis: 0.314 ± 0.02	1.446HisIle: 1.446 ± 0.036	1.095HisLys: 1.095 ± 0.033	1.178HisLeu: 1.178 ± 0.037	0.408HisMet: 0.408 ± 0.025	0.849HisAsn: 0.849 ± 0.035	0.6HisPro: 0.6 ± 0.028	0.303HisGln: 0.303 ± 0.018	0.606HisArg: 0.606 ± 0.025	0.91HisSer: 0.91 ± 0.033	0.67HisThr: 0.67 ± 0.026	0.825HisVal: 0.825 ± 0.028	0.116HisTrp: 0.116 ± 0.013	0.594HisTyr: 0.594 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	5.919IleAla: 5.919 ± 0.097	1.397IleCys: 1.397 ± 0.046	5.918IleAsp: 5.918 ± 0.092	7.505IleGlu: 7.505 ± 0.096	4.402IlePhe: 4.402 ± 0.096	6.496IleGly: 6.496 ± 0.095	1.335IleHis: 1.335 ± 0.038	9.809IleIle: 9.809 ± 0.147	8.833IleLys: 8.833 ± 0.111	9.236IleLeu: 9.236 ± 0.121	2.506IleMet: 2.506 ± 0.055	6.388IleAsn: 6.388 ± 0.102	3.376IlePro: 3.376 ± 0.065	2.075IleGln: 2.075 ± 0.051	3.135IleArg: 3.135 ± 0.057	7.254IleSer: 7.254 ± 0.109	5.185IleThr: 5.185 ± 0.076	6.412IleVal: 6.412 ± 0.078	0.541IleTrp: 0.541 ± 0.026	3.712IleTyr: 3.712 ± 0.079	0.0IleXaa: 0.0 ± 0.0
Lys	4.742LysAla: 4.742 ± 0.088	0.934LysCys: 0.934 ± 0.036	5.682LysAsp: 5.682 ± 0.087	8.96LysGlu: 8.96 ± 0.118	3.323LysPhe: 3.323 ± 0.068	5.275LysGly: 5.275 ± 0.079	1.13LysHis: 1.13 ± 0.036	7.763LysIle: 7.763 ± 0.096	7.246LysLys: 7.246 ± 0.103	7.488LysLeu: 7.488 ± 0.088	2.241LysMet: 2.241 ± 0.043	5.894LysAsn: 5.894 ± 0.086	2.014LysPro: 2.014 ± 0.049	1.909LysGln: 1.909 ± 0.054	3.032LysArg: 3.032 ± 0.056	5.187LysSer: 5.187 ± 0.069	3.92LysThr: 3.92 ± 0.066	6.093LysVal: 6.093 ± 0.085	0.591LysTrp: 0.591 ± 0.027	3.993LysTyr: 3.993 ± 0.064	0.0LysXaa: 0.0 ± 0.0
Leu	4.985LeuAla: 4.985 ± 0.081	1.229LeuCys: 1.229 ± 0.032	5.082LeuAsp: 5.082 ± 0.077	6.373LeuGlu: 6.373 ± 0.092	3.621LeuPhe: 3.621 ± 0.078	6.2LeuGly: 6.2 ± 0.089	1.152LeuHis: 1.152 ± 0.036	8.396LeuIle: 8.396 ± 0.115	8.383LeuLys: 8.383 ± 0.109	7.897LeuLeu: 7.897 ± 0.114	2.361LeuMet: 2.361 ± 0.05	5.598LeuAsn: 5.598 ± 0.089	2.716LeuPro: 2.716 ± 0.056	2.154LeuGln: 2.154 ± 0.05	3.24LeuArg: 3.24 ± 0.065	6.596LeuSer: 6.596 ± 0.094	4.412LeuThr: 4.412 ± 0.062	5.55LeuVal: 5.55 ± 0.087	0.613LeuTrp: 0.613 ± 0.029	3.294LeuTyr: 3.294 ± 0.066	0.0LeuXaa: 0.0 ± 0.0
Met	1.755MetAla: 1.755 ± 0.048	0.326MetCys: 0.326 ± 0.02	1.701MetAsp: 1.701 ± 0.043	2.181MetGlu: 2.181 ± 0.048	1.085MetPhe: 1.085 ± 0.033	1.965MetGly: 1.965 ± 0.052	0.316MetHis: 0.316 ± 0.02	2.395MetIle: 2.395 ± 0.057	2.628MetLys: 2.628 ± 0.051	2.177MetLeu: 2.177 ± 0.049	0.727MetMet: 0.727 ± 0.028	1.8MetAsn: 1.8 ± 0.044	0.914MetPro: 0.914 ± 0.031	0.612MetGln: 0.612 ± 0.028	0.939MetArg: 0.939 ± 0.032	1.716MetSer: 1.716 ± 0.049	1.186MetThr: 1.186 ± 0.034	1.753MetVal: 1.753 ± 0.048	0.18MetTrp: 0.18 ± 0.014	0.868MetTyr: 0.868 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	2.675AsnAla: 2.675 ± 0.051	0.829AsnCys: 0.829 ± 0.035	2.726AsnAsp: 2.726 ± 0.062	4.277AsnGlu: 4.277 ± 0.079	2.589AsnPhe: 2.589 ± 0.06	3.502AsnGly: 3.502 ± 0.06	0.934AsnHis: 0.934 ± 0.03	7.08AsnIle: 7.08 ± 0.099	5.798AsnLys: 5.798 ± 0.093	5.544AsnLeu: 5.544 ± 0.084	1.73AsnMet: 1.73 ± 0.046	4.205AsnAsn: 4.205 ± 0.089	2.114AsnPro: 2.114 ± 0.051	1.328AsnGln: 1.328 ± 0.04	2.093AsnArg: 2.093 ± 0.05	3.913AsnSer: 3.913 ± 0.072	2.935AsnThr: 2.935 ± 0.061	3.472AsnVal: 3.472 ± 0.055	0.415AsnTrp: 0.415 ± 0.025	2.839AsnTyr: 2.839 ± 0.067	0.0AsnXaa: 0.0 ± 0.0
Pro	1.358ProAla: 1.358 ± 0.04	0.345ProCys: 0.345 ± 0.022	1.328ProAsp: 1.328 ± 0.039	2.143ProGlu: 2.143 ± 0.054	1.297ProPhe: 1.297 ± 0.035	1.697ProGly: 1.697 ± 0.048	0.486ProHis: 0.486 ± 0.022	2.791ProIle: 2.791 ± 0.058	2.211ProLys: 2.211 ± 0.052	2.52ProLeu: 2.52 ± 0.055	0.814ProMet: 0.814 ± 0.031	1.485ProAsn: 1.485 ± 0.05	0.604ProPro: 0.604 ± 0.026	0.723ProGln: 0.723 ± 0.027	0.854ProArg: 0.854 ± 0.031	1.765ProSer: 1.765 ± 0.039	1.552ProThr: 1.552 ± 0.039	2.005ProVal: 2.005 ± 0.052	0.24ProTrp: 0.24 ± 0.016	1.268ProTyr: 1.268 ± 0.035	0.0ProXaa: 0.0 ± 0.0
Gln	1.18GlnAla: 1.18 ± 0.039	0.398GlnCys: 0.398 ± 0.022	1.169GlnAsp: 1.169 ± 0.037	1.593GlnGlu: 1.593 ± 0.044	0.858GlnPhe: 0.858 ± 0.027	1.705GlnGly: 1.705 ± 0.042	0.335GlnHis: 0.335 ± 0.018	1.996GlnIle: 1.996 ± 0.048	1.637GlnLys: 1.637 ± 0.046	2.056GlnLeu: 2.056 ± 0.048	0.592GlnMet: 0.592 ± 0.026	1.164GlnAsn: 1.164 ± 0.04	0.57GlnPro: 0.57 ± 0.025	0.66GlnGln: 0.66 ± 0.033	0.974GlnArg: 0.974 ± 0.036	1.311GlnSer: 1.311 ± 0.037	0.889GlnThr: 0.889 ± 0.032	1.719GlnVal: 1.719 ± 0.037	0.314GlnTrp: 0.314 ± 0.02	0.946GlnTyr: 0.946 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	1.959ArgAla: 1.959 ± 0.048	0.435ArgCys: 0.435 ± 0.021	1.934ArgAsp: 1.934 ± 0.047	3.097ArgGlu: 3.097 ± 0.059	1.434ArgPhe: 1.434 ± 0.038	2.227ArgGly: 2.227 ± 0.048	0.483ArgHis: 0.483 ± 0.022	3.226ArgIle: 3.226 ± 0.058	3.053ArgLys: 3.053 ± 0.056	2.87ArgLeu: 2.87 ± 0.06	0.981ArgMet: 0.981 ± 0.033	2.148ArgAsn: 2.148 ± 0.049	0.809ArgPro: 0.809 ± 0.033	0.875ArgGln: 0.875 ± 0.031	1.466ArgArg: 1.466 ± 0.04	1.652ArgSer: 1.652 ± 0.043	1.516ArgThr: 1.516 ± 0.045	2.509ArgVal: 2.509 ± 0.049	0.216ArgTrp: 0.216 ± 0.016	1.491ArgTyr: 1.491 ± 0.04	0.0ArgXaa: 0.0 ± 0.0
Ser	3.127SerAla: 3.127 ± 0.058	0.694SerCys: 0.694 ± 0.032	3.054SerAsp: 3.054 ± 0.057	4.098SerGlu: 4.098 ± 0.074	3.02SerPhe: 3.02 ± 0.055	4.243SerGly: 4.243 ± 0.08	0.962SerHis: 0.962 ± 0.032	6.876SerIle: 6.876 ± 0.1	5.78SerLys: 5.78 ± 0.08	5.821SerLeu: 5.821 ± 0.092	1.833SerMet: 1.833 ± 0.05	3.683SerAsn: 3.683 ± 0.071	1.619SerPro: 1.619 ± 0.047	1.584SerGln: 1.584 ± 0.042	2.16SerArg: 2.16 ± 0.041	4.405SerSer: 4.405 ± 0.084	3.264SerThr: 3.264 ± 0.056	3.739SerVal: 3.739 ± 0.068	0.436SerTrp: 0.436 ± 0.026	2.616SerTyr: 2.616 ± 0.06	0.0SerXaa: 0.0 ± 0.0
Thr	2.787ThrAla: 2.787 ± 0.064	0.586ThrCys: 0.586 ± 0.022	2.21ThrAsp: 2.21 ± 0.048	3.116ThrGlu: 3.116 ± 0.06	2.237ThrPhe: 2.237 ± 0.05	3.538ThrGly: 3.538 ± 0.073	0.779ThrHis: 0.779 ± 0.028	5.05ThrIle: 5.05 ± 0.08	3.575ThrLys: 3.575 ± 0.062	5.136ThrLeu: 5.136 ± 0.076	1.289ThrMet: 1.289 ± 0.043	2.651ThrAsn: 2.651 ± 0.054	1.804ThrPro: 1.804 ± 0.04	1.034ThrGln: 1.034 ± 0.031	1.555ThrArg: 1.555 ± 0.045	3.222ThrSer: 3.222 ± 0.066	2.826ThrThr: 2.826 ± 0.059	3.469ThrVal: 3.469 ± 0.059	0.327ThrTrp: 0.327 ± 0.019	1.955ThrTyr: 1.955 ± 0.05	0.0ThrXaa: 0.0 ± 0.0
Val	4.128ValAla: 4.128 ± 0.09	0.89ValCys: 0.89 ± 0.029	3.983ValAsp: 3.983 ± 0.074	4.787ValGlu: 4.787 ± 0.084	2.841ValPhe: 2.841 ± 0.054	4.412ValGly: 4.412 ± 0.067	0.903ValHis: 0.903 ± 0.036	6.557ValIle: 6.557 ± 0.075	5.484ValLys: 5.484 ± 0.091	5.922ValLeu: 5.922 ± 0.087	1.717ValMet: 1.717 ± 0.042	3.685ValAsn: 3.685 ± 0.061	1.953ValPro: 1.953 ± 0.046	1.417ValGln: 1.417 ± 0.042	2.153ValArg: 2.153 ± 0.047	4.212ValSer: 4.212 ± 0.067	3.426ValThr: 3.426 ± 0.067	4.939ValVal: 4.939 ± 0.081	0.362ValTrp: 0.362 ± 0.021	2.375ValTyr: 2.375 ± 0.049	0.0ValXaa: 0.0 ± 0.0
Trp	0.371TrpAla: 0.371 ± 0.021	0.111TrpCys: 0.111 ± 0.011	0.339TrpAsp: 0.339 ± 0.019	0.45TrpGlu: 0.45 ± 0.023	0.321TrpPhe: 0.321 ± 0.019	0.508TrpGly: 0.508 ± 0.027	0.125TrpHis: 0.125 ± 0.013	0.703TrpIle: 0.703 ± 0.028	0.504TrpLys: 0.504 ± 0.025	0.573TrpLeu: 0.573 ± 0.025	0.201TrpMet: 0.201 ± 0.015	0.418TrpAsn: 0.418 ± 0.021	0.159TrpPro: 0.159 ± 0.011	0.204TrpGln: 0.204 ± 0.013	0.244TrpArg: 0.244 ± 0.019	0.416TrpSer: 0.416 ± 0.021	0.295TrpThr: 0.295 ± 0.018	0.431TrpVal: 0.431 ± 0.027	0.08TrpTrp: 0.08 ± 0.011	0.259TrpTyr: 0.259 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.88TyrAla: 1.88 ± 0.045	0.599TyrCys: 0.599 ± 0.025	2.44TyrAsp: 2.44 ± 0.054	3.081TyrGlu: 3.081 ± 0.065	1.992TyrPhe: 1.992 ± 0.052	2.788TyrGly: 2.788 ± 0.049	0.6TyrHis: 0.6 ± 0.026	4.192TyrIle: 4.192 ± 0.079	3.669TyrLys: 3.669 ± 0.073	3.554TyrLeu: 3.554 ± 0.066	1.026TyrMet: 1.026 ± 0.033	2.828TyrAsn: 2.828 ± 0.069	1.253TyrPro: 1.253 ± 0.034	0.708TyrGln: 0.708 ± 0.029	1.476TyrArg: 1.476 ± 0.043	2.72TyrSer: 2.72 ± 0.06	2.024TyrThr: 2.024 ± 0.047	2.4TyrVal: 2.4 ± 0.054	0.308TyrTrp: 0.308 ± 0.02	1.779TyrTyr: 1.779 ± 0.049	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3148 proteins (922629 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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