Staphylococcus phage StAP1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Twortvirinae; Silviavirus; unclassified Silviavirus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 192 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1P8CMY7|A0A1P8CMY7_9CAUD Uncharacterized protein OS=Staphylococcus phage StAP1 OX=1913667 GN=SAP1_151 PE=4 SV=1
MM1 pKa = 7.7LEE3 pKa = 3.83QAKK6 pKa = 10.69GITTDD11 pKa = 3.86YY12 pKa = 10.97IMEE15 pKa = 5.36FIDD18 pKa = 3.39EE19 pKa = 4.46HH20 pKa = 4.75EE21 pKa = 4.41HH22 pKa = 6.22YY23 pKa = 10.25FYY25 pKa = 11.48EE26 pKa = 5.01DD27 pKa = 3.21LHH29 pKa = 8.19KK30 pKa = 10.89LAGEE34 pKa = 4.14LVLKK38 pKa = 8.97DD39 pKa = 3.47THH41 pKa = 6.45DD42 pKa = 3.75TNPIVINDD50 pKa = 3.41GDD52 pKa = 4.5GYY54 pKa = 10.99SGTIISKK61 pKa = 10.05FKK63 pKa = 11.0SKK65 pKa = 11.26VEE67 pKa = 3.99GVPDD71 pKa = 3.21ITVSDD76 pKa = 4.67FYY78 pKa = 11.38GSCSFCDD85 pKa = 3.32TLTNVYY91 pKa = 9.96EE92 pKa = 4.95DD93 pKa = 3.69SDD95 pKa = 3.81EE96 pKa = 4.64LEE98 pKa = 4.69IIRR101 pKa = 11.84DD102 pKa = 3.54LATMVLHH109 pKa = 7.14TIQSMEE115 pKa = 4.15EE116 pKa = 3.75QVEE119 pKa = 4.09

Molecular weight: 13.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1P8CMW4|A0A1P8CMW4_9CAUD Uncharacterized protein OS=Staphylococcus phage StAP1 OX=1913667 GN=SAP1_126 PE=4 SV=1
MM1 pKa = 7.92AKK3 pKa = 10.16RR4 pKa = 11.84KK5 pKa = 9.45PDD7 pKa = 3.34TFKK10 pKa = 10.96PKK12 pKa = 10.48SNGYY16 pKa = 8.74KK17 pKa = 9.38HH18 pKa = 6.23QPVNFAPTGNLSGRR32 pKa = 11.84STSFFNKK39 pKa = 8.92KK40 pKa = 10.13RR41 pKa = 11.84KK42 pKa = 9.8DD43 pKa = 3.44IPDD46 pKa = 3.17EE47 pKa = 4.68SIVVKK52 pKa = 9.57YY53 pKa = 10.54KK54 pKa = 10.22PLFVKK59 pKa = 10.62RR60 pKa = 11.84FDD62 pKa = 3.7NVTATDD68 pKa = 3.21IKK70 pKa = 10.4IQKK73 pKa = 9.87KK74 pKa = 9.47YY75 pKa = 10.88ALDD78 pKa = 4.92LISEE82 pKa = 4.45AVNIKK87 pKa = 10.21KK88 pKa = 10.28KK89 pKa = 10.28YY90 pKa = 10.83LVMKK94 pKa = 10.42QKK96 pKa = 10.94GKK98 pKa = 8.7LTQTILHH105 pKa = 6.46TDD107 pKa = 3.01RR108 pKa = 11.84VYY110 pKa = 10.94YY111 pKa = 9.78VYY113 pKa = 10.42RR114 pKa = 11.84GKK116 pKa = 10.86KK117 pKa = 10.24LIGKK121 pKa = 9.5CSIRR125 pKa = 11.84EE126 pKa = 3.68QRR128 pKa = 11.84TFRR131 pKa = 11.84GTHH134 pKa = 6.92LIYY137 pKa = 10.41IFSTRR142 pKa = 11.84HH143 pKa = 5.57RR144 pKa = 11.84ISVRR148 pKa = 11.84KK149 pKa = 9.89NSRR152 pKa = 11.84LDD154 pKa = 3.27KK155 pKa = 10.92KK156 pKa = 9.88RR157 pKa = 11.84TPKK160 pKa = 10.7KK161 pKa = 9.75MIYY164 pKa = 9.87KK165 pKa = 10.11GGKK168 pKa = 8.35

Molecular weight: 19.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

192

0

192

38692

34

1152

201.5

23.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.918 ± 0.163

0.744 ± 0.066

6.88 ± 0.152

7.663 ± 0.243

3.722 ± 0.114

5.221 ± 0.247

1.652 ± 0.088

7.487 ± 0.197

9.839 ± 0.236

8.449 ± 0.194

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.316 ± 0.086

6.895 ± 0.18

2.595 ± 0.167

3.502 ± 0.161

3.605 ± 0.143

6.51 ± 0.175

6.397 ± 0.209

6.381 ± 0.172

0.855 ± 0.067

5.368 ± 0.17
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski