Amino acid dipepetide frequency for Staphylococcus phage StAP1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.052AlaAla: 0.052 ± 0.038
0.439AlaCys: 0.439 ± 0.102
2.274AlaAsp: 2.274 ± 0.303
2.74AlaGlu: 2.74 ± 0.351
1.525AlaPhe: 1.525 ± 0.218
1.654AlaGly: 1.654 ± 0.287
1.06AlaHis: 1.06 ± 0.163
3.205AlaIle: 3.205 ± 0.274
4.445AlaLys: 4.445 ± 0.394
3.231AlaLeu: 3.231 ± 0.281
0.982AlaMet: 0.982 ± 0.2
2.3AlaAsn: 2.3 ± 0.3
1.241AlaPro: 1.241 ± 0.187
1.551AlaGln: 1.551 ± 0.223
1.37AlaArg: 1.37 ± 0.155
3.412AlaSer: 3.412 ± 0.365
2.817AlaThr: 2.817 ± 0.284
2.455AlaVal: 2.455 ± 0.254
0.414AlaTrp: 0.414 ± 0.111
2.016AlaTyr: 2.016 ± 0.189
0.0AlaXaa: 0.0 ± 0.0
Cys
0.284CysAla: 0.284 ± 0.082
0.103CysCys: 0.103 ± 0.058
0.336CysAsp: 0.336 ± 0.09
0.491CysGlu: 0.491 ± 0.123
0.284CysPhe: 0.284 ± 0.086
0.517CysGly: 0.517 ± 0.125
0.078CysHis: 0.078 ± 0.043
0.491CysIle: 0.491 ± 0.102
0.905CysLys: 0.905 ± 0.173
0.827CysLeu: 0.827 ± 0.16
0.129CysMet: 0.129 ± 0.058
0.388CysAsn: 0.388 ± 0.118
0.258CysPro: 0.258 ± 0.088
0.155CysGln: 0.155 ± 0.059
0.336CysArg: 0.336 ± 0.096
0.569CysSer: 0.569 ± 0.149
0.207CysThr: 0.207 ± 0.077
0.414CysVal: 0.414 ± 0.1
0.103CysTrp: 0.103 ± 0.05
0.569CysTyr: 0.569 ± 0.131
0.0CysXaa: 0.0 ± 0.0
Asp
2.352AspAla: 2.352 ± 0.298
0.439AspCys: 0.439 ± 0.112
4.213AspAsp: 4.213 ± 0.386
4.342AspGlu: 4.342 ± 0.341
3.101AspPhe: 3.101 ± 0.266
3.877AspGly: 3.877 ± 0.344
0.491AspHis: 0.491 ± 0.118
7.004AspIle: 7.004 ± 0.496
7.133AspLys: 7.133 ± 0.403
6.332AspLeu: 6.332 ± 0.421
1.887AspMet: 1.887 ± 0.209
5.479AspAsn: 5.479 ± 0.286
1.318AspPro: 1.318 ± 0.169
0.775AspGln: 0.775 ± 0.18
2.636AspArg: 2.636 ± 0.251
3.67AspSer: 3.67 ± 0.358
4.42AspThr: 4.42 ± 0.27
4.575AspVal: 4.575 ± 0.347
0.698AspTrp: 0.698 ± 0.121
4.058AspTyr: 4.058 ± 0.33
0.0AspXaa: 0.0 ± 0.0
Glu
3.334GluAla: 3.334 ± 0.35
0.414GluCys: 0.414 ± 0.111
5.583GluAsp: 5.583 ± 0.36
6.72GluGlu: 6.72 ± 0.684
2.662GluPhe: 2.662 ± 0.262
3.334GluGly: 3.334 ± 0.272
1.551GluHis: 1.551 ± 0.224
4.497GluIle: 4.497 ± 0.331
6.901GluLys: 6.901 ± 0.602
8.167GluLeu: 8.167 ± 0.591
2.119GluMet: 2.119 ± 0.227
4.187GluAsn: 4.187 ± 0.374
1.758GluPro: 1.758 ± 0.413
4.084GluGln: 4.084 ± 0.419
2.636GluArg: 2.636 ± 0.272
4.445GluSer: 4.445 ± 0.318
3.463GluThr: 3.463 ± 0.276
6.177GluVal: 6.177 ± 0.388
0.569GluTrp: 0.569 ± 0.115
4.032GluTyr: 4.032 ± 0.357
0.0GluXaa: 0.0 ± 0.0
Phe
1.163PheAla: 1.163 ± 0.168
0.388PheCys: 0.388 ± 0.09
2.223PheAsp: 2.223 ± 0.253
2.843PheGlu: 2.843 ± 0.259
1.215PhePhe: 1.215 ± 0.179
2.249PheGly: 2.249 ± 0.241
0.439PheHis: 0.439 ± 0.095
3.515PheIle: 3.515 ± 0.312
3.851PheLys: 3.851 ± 0.304
2.869PheLeu: 2.869 ± 0.264
0.93PheMet: 0.93 ± 0.155
3.386PheAsn: 3.386 ± 0.282
0.982PhePro: 0.982 ± 0.195
0.827PheGln: 0.827 ± 0.112
0.905PheArg: 0.905 ± 0.162
2.817PheSer: 2.817 ± 0.27
2.171PheThr: 2.171 ± 0.25
1.99PheVal: 1.99 ± 0.233
0.233PheTrp: 0.233 ± 0.086
2.197PheTyr: 2.197 ± 0.276
0.0PheXaa: 0.0 ± 0.0
Gly
2.197GlyAla: 2.197 ± 0.41
0.439GlyCys: 0.439 ± 0.111
3.386GlyAsp: 3.386 ± 0.373
3.205GlyGlu: 3.205 ± 0.313
1.809GlyPhe: 1.809 ± 0.215
3.567GlyGly: 3.567 ± 0.641
0.93GlyHis: 0.93 ± 0.145
4.239GlyIle: 4.239 ± 0.318
5.428GlyLys: 5.428 ± 0.543
3.825GlyLeu: 3.825 ± 0.323
1.163GlyMet: 1.163 ± 0.175
3.386GlyAsn: 3.386 ± 0.287
0.0GlyPro: 0.0 ± 0.0
1.706GlyGln: 1.706 ± 0.246
2.094GlyArg: 2.094 ± 0.237
3.437GlySer: 3.437 ± 0.343
3.773GlyThr: 3.773 ± 0.31
3.282GlyVal: 3.282 ± 0.35
0.646GlyTrp: 0.646 ± 0.166
3.696GlyTyr: 3.696 ± 0.297
0.0GlyXaa: 0.0 ± 0.0
His
0.594HisAla: 0.594 ± 0.104
0.233HisCys: 0.233 ± 0.079
0.879HisAsp: 0.879 ± 0.151
0.905HisGlu: 0.905 ± 0.152
0.672HisPhe: 0.672 ± 0.137
1.008HisGly: 1.008 ± 0.168
0.258HisHis: 0.258 ± 0.081
1.473HisIle: 1.473 ± 0.219
1.706HisLys: 1.706 ± 0.209
1.266HisLeu: 1.266 ± 0.217
0.569HisMet: 0.569 ± 0.135
1.137HisAsn: 1.137 ± 0.192
0.388HisPro: 0.388 ± 0.113
0.491HisGln: 0.491 ± 0.11
0.62HisArg: 0.62 ± 0.126
0.93HisSer: 0.93 ± 0.14
1.008HisThr: 1.008 ± 0.168
1.422HisVal: 1.422 ± 0.228
0.103HisTrp: 0.103 ± 0.058
0.853HisTyr: 0.853 ± 0.137
0.0HisXaa: 0.0 ± 0.0
Ile
2.585IleAla: 2.585 ± 0.277
0.646IleCys: 0.646 ± 0.151
6.436IleAsp: 6.436 ± 0.365
6.151IleGlu: 6.151 ± 0.49
2.042IlePhe: 2.042 ± 0.25
3.722IleGly: 3.722 ± 0.288
0.982IleHis: 0.982 ± 0.181
5.583IleIle: 5.583 ± 0.478
7.495IleLys: 7.495 ± 0.453
5.712IleLeu: 5.712 ± 0.405
1.602IleMet: 1.602 ± 0.207
5.893IleAsn: 5.893 ± 0.491
2.274IlePro: 2.274 ± 0.234
2.688IleGln: 2.688 ± 0.227
2.507IleArg: 2.507 ± 0.256
4.652IleSer: 4.652 ± 0.296
5.686IleThr: 5.686 ± 0.396
4.549IleVal: 4.549 ± 0.405
0.491IleTrp: 0.491 ± 0.115
3.179IleTyr: 3.179 ± 0.269
0.0IleXaa: 0.0 ± 0.0
Lys
3.773LysAla: 3.773 ± 0.408
0.75LysCys: 0.75 ± 0.197
8.064LysAsp: 8.064 ± 0.482
10.545LysGlu: 10.545 ± 0.698
3.101LysPhe: 3.101 ± 0.252
6.177LysGly: 6.177 ± 0.571
2.326LysHis: 2.326 ± 0.281
4.42LysIle: 4.42 ± 0.372
8.245LysLys: 8.245 ± 0.636
7.702LysLeu: 7.702 ± 0.543
1.835LysMet: 1.835 ± 0.212
6.125LysAsn: 6.125 ± 0.432
3.05LysPro: 3.05 ± 0.487
3.877LysGln: 3.877 ± 0.354
4.109LysArg: 4.109 ± 0.363
5.634LysSer: 5.634 ± 0.457
5.324LysThr: 5.324 ± 0.381
6.746LysVal: 6.746 ± 0.452
0.724LysTrp: 0.724 ± 0.141
5.867LysTyr: 5.867 ± 0.451
0.0LysXaa: 0.0 ± 0.0
Leu
3.773LeuAla: 3.773 ± 0.344
0.517LeuCys: 0.517 ± 0.11
6.229LeuAsp: 6.229 ± 0.471
6.797LeuGlu: 6.797 ± 0.429
3.127LeuPhe: 3.127 ± 0.36
4.29LeuGly: 4.29 ± 0.483
1.37LeuHis: 1.37 ± 0.202
5.376LeuIle: 5.376 ± 0.41
8.4LeuLys: 8.4 ± 0.383
6.72LeuLeu: 6.72 ± 0.513
2.197LeuMet: 2.197 ± 0.244
5.117LeuAsn: 5.117 ± 0.359
2.843LeuPro: 2.843 ± 0.264
3.515LeuGln: 3.515 ± 0.299
3.231LeuArg: 3.231 ± 0.275
6.41LeuSer: 6.41 ± 0.419
5.557LeuThr: 5.557 ± 0.42
4.549LeuVal: 4.549 ± 0.388
0.569LeuTrp: 0.569 ± 0.113
3.903LeuTyr: 3.903 ± 0.308
0.0LeuXaa: 0.0 ± 0.0
Met
1.241MetAla: 1.241 ± 0.21
0.155MetCys: 0.155 ± 0.066
1.344MetAsp: 1.344 ± 0.189
1.783MetGlu: 1.783 ± 0.192
0.956MetPhe: 0.956 ± 0.181
0.879MetGly: 0.879 ± 0.195
0.336MetHis: 0.336 ± 0.104
1.783MetIle: 1.783 ± 0.226
2.378MetLys: 2.378 ± 0.247
1.577MetLeu: 1.577 ± 0.204
0.594MetMet: 0.594 ± 0.165
1.189MetAsn: 1.189 ± 0.171
0.517MetPro: 0.517 ± 0.113
0.827MetGln: 0.827 ± 0.124
0.853MetArg: 0.853 ± 0.156
2.274MetSer: 2.274 ± 0.242
1.318MetThr: 1.318 ± 0.17
1.447MetVal: 1.447 ± 0.199
0.284MetTrp: 0.284 ± 0.07
1.422MetTyr: 1.422 ± 0.202
0.0MetXaa: 0.0 ± 0.0
Asn
2.533AsnAla: 2.533 ± 0.248
0.414AsnCys: 0.414 ± 0.099
3.593AsnAsp: 3.593 ± 0.289
4.652AsnGlu: 4.652 ± 0.443
2.352AsnPhe: 2.352 ± 0.23
3.463AsnGly: 3.463 ± 0.263
1.292AsnHis: 1.292 ± 0.178
5.531AsnIle: 5.531 ± 0.438
8.167AsnLys: 8.167 ± 0.425
5.945AsnLeu: 5.945 ± 0.446
1.422AsnMet: 1.422 ± 0.183
5.764AsnAsn: 5.764 ± 0.414
2.352AsnPro: 2.352 ± 0.284
2.507AsnGln: 2.507 ± 0.364
2.559AsnArg: 2.559 ± 0.292
3.515AsnSer: 3.515 ± 0.237
5.273AsnThr: 5.273 ± 0.433
3.851AsnVal: 3.851 ± 0.319
0.594AsnTrp: 0.594 ± 0.146
3.179AsnTyr: 3.179 ± 0.303
0.0AsnXaa: 0.0 ± 0.0
Pro
1.008ProAla: 1.008 ± 0.139
0.155ProCys: 0.155 ± 0.067
1.422ProAsp: 1.422 ± 0.189
1.99ProGlu: 1.99 ± 0.373
1.008ProPhe: 1.008 ± 0.161
0.594ProGly: 0.594 ± 0.164
0.362ProHis: 0.362 ± 0.084
2.43ProIle: 2.43 ± 0.467
2.688ProLys: 2.688 ± 0.259
2.274ProLeu: 2.274 ± 0.259
0.569ProMet: 0.569 ± 0.113
2.094ProAsn: 2.094 ± 0.286
0.569ProPro: 0.569 ± 0.15
1.086ProGln: 1.086 ± 0.198
0.853ProArg: 0.853 ± 0.151
1.577ProSer: 1.577 ± 0.198
2.171ProThr: 2.171 ± 0.257
1.215ProVal: 1.215 ± 0.193
0.078ProTrp: 0.078 ± 0.049
1.809ProTyr: 1.809 ± 0.206
0.0ProXaa: 0.0 ± 0.0
Gln
1.913GlnAla: 1.913 ± 0.263
0.233GlnCys: 0.233 ± 0.087
2.662GlnAsp: 2.662 ± 0.246
3.282GlnGlu: 3.282 ± 0.43
1.111GlnPhe: 1.111 ± 0.203
2.274GlnGly: 2.274 ± 0.266
0.491GlnHis: 0.491 ± 0.113
2.171GlnIle: 2.171 ± 0.251
2.636GlnLys: 2.636 ± 0.319
3.282GlnLeu: 3.282 ± 0.286
0.853GlnMet: 0.853 ± 0.147
1.577GlnAsn: 1.577 ± 0.198
0.801GlnPro: 0.801 ± 0.165
1.887GlnGln: 1.887 ± 0.365
1.292GlnArg: 1.292 ± 0.181
2.585GlnSer: 2.585 ± 0.278
1.654GlnThr: 1.654 ± 0.193
2.481GlnVal: 2.481 ± 0.246
0.284GlnTrp: 0.284 ± 0.077
1.551GlnTyr: 1.551 ± 0.184
0.0GlnXaa: 0.0 ± 0.0
Arg
1.628ArgAla: 1.628 ± 0.216
0.362ArgCys: 0.362 ± 0.097
2.61ArgAsp: 2.61 ± 0.252
2.43ArgGlu: 2.43 ± 0.237
2.068ArgPhe: 2.068 ± 0.203
1.99ArgGly: 1.99 ± 0.252
0.543ArgHis: 0.543 ± 0.114
2.145ArgIle: 2.145 ± 0.194
3.618ArgLys: 3.618 ± 0.37
3.231ArgLeu: 3.231 ± 0.289
1.06ArgMet: 1.06 ± 0.158
2.042ArgAsn: 2.042 ± 0.195
0.879ArgPro: 0.879 ± 0.134
1.241ArgGln: 1.241 ± 0.154
1.628ArgArg: 1.628 ± 0.233
1.628ArgSer: 1.628 ± 0.199
2.378ArgThr: 2.378 ± 0.246
2.688ArgVal: 2.688 ± 0.297
0.362ArgTrp: 0.362 ± 0.11
1.525ArgTyr: 1.525 ± 0.189
0.0ArgXaa: 0.0 ± 0.0
Ser
2.533SerAla: 2.533 ± 0.274
0.414SerCys: 0.414 ± 0.098
4.368SerAsp: 4.368 ± 0.382
4.135SerGlu: 4.135 ± 0.299
3.024SerPhe: 3.024 ± 0.277
3.101SerGly: 3.101 ± 0.382
0.853SerHis: 0.853 ± 0.148
5.893SerIle: 5.893 ± 0.4
6.823SerLys: 6.823 ± 0.456
5.169SerLeu: 5.169 ± 0.443
1.189SerMet: 1.189 ± 0.173
5.505SerAsn: 5.505 ± 0.413
1.292SerPro: 1.292 ± 0.186
1.758SerGln: 1.758 ± 0.187
2.016SerArg: 2.016 ± 0.216
5.014SerSer: 5.014 ± 0.449
4.394SerThr: 4.394 ± 0.403
3.515SerVal: 3.515 ± 0.332
0.594SerTrp: 0.594 ± 0.125
3.515SerTyr: 3.515 ± 0.359
0.0SerXaa: 0.0 ± 0.0
Thr
2.895ThrAla: 2.895 ± 0.323
0.233ThrCys: 0.233 ± 0.083
4.084ThrAsp: 4.084 ± 0.325
4.471ThrGlu: 4.471 ± 0.35
2.817ThrPhe: 2.817 ± 0.265
3.101ThrGly: 3.101 ± 0.245
1.241ThrHis: 1.241 ± 0.157
5.35ThrIle: 5.35 ± 0.389
5.686ThrLys: 5.686 ± 0.414
5.66ThrLeu: 5.66 ± 0.399
1.292ThrMet: 1.292 ± 0.182
4.342ThrAsn: 4.342 ± 0.303
2.481ThrPro: 2.481 ± 0.263
2.223ThrGln: 2.223 ± 0.291
2.145ThrArg: 2.145 ± 0.252
3.851ThrSer: 3.851 ± 0.303
3.593ThrThr: 3.593 ± 0.365
4.471ThrVal: 4.471 ± 0.398
0.646ThrTrp: 0.646 ± 0.142
3.386ThrTyr: 3.386 ± 0.333
0.0ThrXaa: 0.0 ± 0.0
Val
2.921ValAla: 2.921 ± 0.312
0.62ValCys: 0.62 ± 0.117
4.781ValAsp: 4.781 ± 0.384
5.324ValGlu: 5.324 ± 0.364
2.274ValPhe: 2.274 ± 0.251
3.024ValGly: 3.024 ± 0.26
1.086ValHis: 1.086 ± 0.166
4.575ValIle: 4.575 ± 0.317
5.634ValLys: 5.634 ± 0.332
5.428ValLeu: 5.428 ± 0.433
1.344ValMet: 1.344 ± 0.172
4.109ValAsn: 4.109 ± 0.347
1.758ValPro: 1.758 ± 0.241
1.861ValGln: 1.861 ± 0.206
2.145ValArg: 2.145 ± 0.229
5.143ValSer: 5.143 ± 0.409
4.187ValThr: 4.187 ± 0.337
3.954ValVal: 3.954 ± 0.355
0.414ValTrp: 0.414 ± 0.087
3.231ValTyr: 3.231 ± 0.282
0.0ValXaa: 0.0 ± 0.0
Trp
0.491TrpAla: 0.491 ± 0.13
0.052TrpCys: 0.052 ± 0.035
0.62TrpAsp: 0.62 ± 0.122
0.75TrpGlu: 0.75 ± 0.131
0.388TrpPhe: 0.388 ± 0.107
0.646TrpGly: 0.646 ± 0.15
0.052TrpHis: 0.052 ± 0.039
0.543TrpIle: 0.543 ± 0.119
0.905TrpLys: 0.905 ± 0.17
0.646TrpLeu: 0.646 ± 0.127
0.129TrpMet: 0.129 ± 0.064
0.517TrpAsn: 0.517 ± 0.12
0.0TrpPro: 0.0 ± 0.0
0.233TrpGln: 0.233 ± 0.066
0.129TrpArg: 0.129 ± 0.059
0.491TrpSer: 0.491 ± 0.113
0.414TrpThr: 0.414 ± 0.099
0.672TrpVal: 0.672 ± 0.115
0.207TrpTrp: 0.207 ± 0.085
0.672TrpTyr: 0.672 ± 0.142
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.913TyrAla: 1.913 ± 0.191
0.439TyrCys: 0.439 ± 0.122
3.696TyrAsp: 3.696 ± 0.285
3.076TyrGlu: 3.076 ± 0.342
1.68TyrPhe: 1.68 ± 0.203
2.74TyrGly: 2.74 ± 0.301
0.775TyrHis: 0.775 ± 0.147
4.652TyrIle: 4.652 ± 0.331
5.35TyrLys: 5.35 ± 0.377
4.626TyrLeu: 4.626 ± 0.402
1.266TyrMet: 1.266 ± 0.183
4.42TyrAsn: 4.42 ± 0.354
1.189TyrPro: 1.189 ± 0.199
1.732TyrGln: 1.732 ± 0.2
1.99TyrArg: 1.99 ± 0.226
3.05TyrSer: 3.05 ± 0.268
4.161TyrThr: 4.161 ± 0.425
3.36TyrVal: 3.36 ± 0.286
0.543TyrTrp: 0.543 ± 0.118
3.024TyrTyr: 3.024 ± 0.329
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 192 proteins (38692 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski