Amino acid dipepetide frequency for Gordonia phage MelBins

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
19.492AlaAla: 19.492 ± 2.232
0.988AlaCys: 0.988 ± 0.202
9.772AlaAsp: 9.772 ± 0.887
8.109AlaGlu: 8.109 ± 0.838
2.859AlaPhe: 2.859 ± 0.515
9.408AlaGly: 9.408 ± 0.813
2.131AlaHis: 2.131 ± 0.393
5.458AlaIle: 5.458 ± 0.61
3.171AlaLys: 3.171 ± 0.434
10.396AlaLeu: 10.396 ± 0.789
3.171AlaMet: 3.171 ± 0.413
2.859AlaAsn: 2.859 ± 0.504
6.965AlaPro: 6.965 ± 0.535
4.938AlaGln: 4.938 ± 0.535
10.188AlaArg: 10.188 ± 0.673
5.094AlaSer: 5.094 ± 0.514
7.693AlaThr: 7.693 ± 0.721
8.316AlaVal: 8.316 ± 0.629
2.599AlaTrp: 2.599 ± 0.336
2.651AlaTyr: 2.651 ± 0.32
0.0AlaXaa: 0.0 ± 0.0
Cys
0.884CysAla: 0.884 ± 0.218
0.26CysCys: 0.26 ± 0.151
1.195CysAsp: 1.195 ± 0.325
0.572CysGlu: 0.572 ± 0.194
0.208CysPhe: 0.208 ± 0.084
1.455CysGly: 1.455 ± 0.361
0.312CysHis: 0.312 ± 0.155
0.416CysIle: 0.416 ± 0.191
0.104CysLys: 0.104 ± 0.084
0.26CysLeu: 0.26 ± 0.165
0.156CysMet: 0.156 ± 0.087
0.468CysAsn: 0.468 ± 0.157
1.092CysPro: 1.092 ± 0.288
0.26CysGln: 0.26 ± 0.098
0.884CysArg: 0.884 ± 0.26
0.416CysSer: 0.416 ± 0.149
0.572CysThr: 0.572 ± 0.226
0.312CysVal: 0.312 ± 0.117
0.208CysTrp: 0.208 ± 0.11
0.052CysTyr: 0.052 ± 0.058
0.0CysXaa: 0.0 ± 0.0
Asp
9.98AspAla: 9.98 ± 0.869
1.04AspCys: 1.04 ± 0.265
7.901AspAsp: 7.901 ± 0.914
5.562AspGlu: 5.562 ± 0.728
1.04AspPhe: 1.04 ± 0.231
7.017AspGly: 7.017 ± 0.742
1.559AspHis: 1.559 ± 0.372
1.975AspIle: 1.975 ± 0.27
1.507AspLys: 1.507 ± 0.227
5.354AspLeu: 5.354 ± 0.635
1.611AspMet: 1.611 ± 0.311
1.923AspAsn: 1.923 ± 0.297
5.614AspPro: 5.614 ± 0.698
2.547AspGln: 2.547 ± 0.406
5.042AspArg: 5.042 ± 0.502
2.807AspSer: 2.807 ± 0.418
4.626AspThr: 4.626 ± 0.485
5.302AspVal: 5.302 ± 0.483
1.923AspTrp: 1.923 ± 0.3
1.351AspTyr: 1.351 ± 0.273
0.0AspXaa: 0.0 ± 0.0
Glu
6.289GluAla: 6.289 ± 0.683
0.572GluCys: 0.572 ± 0.183
2.703GluAsp: 2.703 ± 0.482
1.559GluGlu: 1.559 ± 0.407
2.027GluPhe: 2.027 ± 0.282
3.067GluGly: 3.067 ± 0.428
2.079GluHis: 2.079 ± 0.349
2.131GluIle: 2.131 ± 0.335
0.728GluLys: 0.728 ± 0.217
5.666GluLeu: 5.666 ± 0.757
1.403GluMet: 1.403 ± 0.299
1.351GluAsn: 1.351 ± 0.272
3.379GluPro: 3.379 ± 0.462
4.314GluGln: 4.314 ± 0.543
4.158GluArg: 4.158 ± 0.565
2.391GluSer: 2.391 ± 0.379
3.015GluThr: 3.015 ± 0.359
4.522GluVal: 4.522 ± 0.559
1.663GluTrp: 1.663 ± 0.354
1.455GluTyr: 1.455 ± 0.279
0.0GluXaa: 0.0 ± 0.0
Phe
2.807PheAla: 2.807 ± 0.42
0.312PheCys: 0.312 ± 0.133
2.495PheAsp: 2.495 ± 0.379
1.403PheGlu: 1.403 ± 0.29
0.676PhePhe: 0.676 ± 0.272
2.235PheGly: 2.235 ± 0.337
0.624PheHis: 0.624 ± 0.173
0.676PheIle: 0.676 ± 0.22
0.52PheLys: 0.52 ± 0.212
1.559PheLeu: 1.559 ± 0.313
0.416PheMet: 0.416 ± 0.141
0.832PheAsn: 0.832 ± 0.255
1.299PhePro: 1.299 ± 0.29
0.676PheGln: 0.676 ± 0.182
1.507PheArg: 1.507 ± 0.36
0.624PheSer: 0.624 ± 0.17
1.663PheThr: 1.663 ± 0.297
1.923PheVal: 1.923 ± 0.219
0.572PheTrp: 0.572 ± 0.18
0.312PheTyr: 0.312 ± 0.154
0.0PheXaa: 0.0 ± 0.0
Gly
9.668GlyAla: 9.668 ± 1.013
0.572GlyCys: 0.572 ± 0.196
6.133GlyAsp: 6.133 ± 0.648
4.054GlyGlu: 4.054 ± 0.46
1.611GlyPhe: 1.611 ± 0.384
8.472GlyGly: 8.472 ± 1.15
1.923GlyHis: 1.923 ± 0.329
4.21GlyIle: 4.21 ± 0.556
2.495GlyLys: 2.495 ± 0.487
5.198GlyLeu: 5.198 ± 0.554
1.715GlyMet: 1.715 ± 0.35
3.119GlyAsn: 3.119 ± 0.499
3.586GlyPro: 3.586 ± 0.476
3.015GlyGln: 3.015 ± 0.34
5.666GlyArg: 5.666 ± 0.599
4.626GlySer: 4.626 ± 0.654
5.77GlyThr: 5.77 ± 0.885
6.497GlyVal: 6.497 ± 0.66
1.299GlyTrp: 1.299 ± 0.301
2.027GlyTyr: 2.027 ± 0.385
0.0GlyXaa: 0.0 ± 0.0
His
2.391HisAla: 2.391 ± 0.33
0.208HisCys: 0.208 ± 0.115
1.767HisAsp: 1.767 ± 0.297
1.299HisGlu: 1.299 ± 0.22
0.312HisPhe: 0.312 ± 0.132
1.663HisGly: 1.663 ± 0.303
0.572HisHis: 0.572 ± 0.178
1.144HisIle: 1.144 ± 0.292
0.26HisLys: 0.26 ± 0.116
2.287HisLeu: 2.287 ± 0.414
0.78HisMet: 0.78 ± 0.235
0.468HisAsn: 0.468 ± 0.167
1.559HisPro: 1.559 ± 0.328
0.78HisGln: 0.78 ± 0.184
2.391HisArg: 2.391 ± 0.411
0.572HisSer: 0.572 ± 0.201
1.611HisThr: 1.611 ± 0.333
1.767HisVal: 1.767 ± 0.353
0.208HisTrp: 0.208 ± 0.102
0.52HisTyr: 0.52 ± 0.174
0.0HisXaa: 0.0 ± 0.0
Ile
6.289IleAla: 6.289 ± 0.578
0.416IleCys: 0.416 ± 0.209
3.742IleAsp: 3.742 ± 0.408
3.067IleGlu: 3.067 ± 0.39
0.416IlePhe: 0.416 ± 0.166
4.21IleGly: 4.21 ± 0.674
0.468IleHis: 0.468 ± 0.16
1.507IleIle: 1.507 ± 0.327
1.144IleLys: 1.144 ± 0.361
2.495IleLeu: 2.495 ± 0.408
0.52IleMet: 0.52 ± 0.138
1.767IleAsn: 1.767 ± 0.248
2.703IlePro: 2.703 ± 0.371
1.092IleGln: 1.092 ± 0.277
2.391IleArg: 2.391 ± 0.345
1.247IleSer: 1.247 ± 0.315
3.327IleThr: 3.327 ± 0.39
4.106IleVal: 4.106 ± 0.499
0.728IleTrp: 0.728 ± 0.186
0.78IleTyr: 0.78 ± 0.19
0.0IleXaa: 0.0 ± 0.0
Lys
3.742LysAla: 3.742 ± 0.454
0.208LysCys: 0.208 ± 0.107
1.144LysAsp: 1.144 ± 0.28
0.52LysGlu: 0.52 ± 0.169
0.884LysPhe: 0.884 ± 0.27
1.507LysGly: 1.507 ± 0.31
0.468LysHis: 0.468 ± 0.162
1.092LysIle: 1.092 ± 0.281
0.26LysLys: 0.26 ± 0.103
2.807LysLeu: 2.807 ± 0.349
0.676LysMet: 0.676 ± 0.21
0.884LysAsn: 0.884 ± 0.266
1.715LysPro: 1.715 ± 0.301
0.884LysGln: 0.884 ± 0.332
2.235LysArg: 2.235 ± 0.321
1.455LysSer: 1.455 ± 0.417
1.663LysThr: 1.663 ± 0.334
1.975LysVal: 1.975 ± 0.339
0.208LysTrp: 0.208 ± 0.109
0.936LysTyr: 0.936 ± 0.202
0.0LysXaa: 0.0 ± 0.0
Leu
10.448LeuAla: 10.448 ± 0.671
0.572LeuCys: 0.572 ± 0.198
5.354LeuAsp: 5.354 ± 0.773
5.042LeuGlu: 5.042 ± 0.532
1.923LeuPhe: 1.923 ± 0.333
7.069LeuGly: 7.069 ± 0.997
1.455LeuHis: 1.455 ± 0.312
3.431LeuIle: 3.431 ± 0.466
1.767LeuLys: 1.767 ± 0.335
5.51LeuLeu: 5.51 ± 0.526
1.715LeuMet: 1.715 ± 0.284
2.183LeuAsn: 2.183 ± 0.338
4.99LeuPro: 4.99 ± 0.632
1.715LeuGln: 1.715 ± 0.459
5.354LeuArg: 5.354 ± 0.541
3.431LeuSer: 3.431 ± 0.39
5.51LeuThr: 5.51 ± 0.49
6.549LeuVal: 6.549 ± 0.586
1.611LeuTrp: 1.611 ± 0.34
1.455LeuTyr: 1.455 ± 0.268
0.0LeuXaa: 0.0 ± 0.0
Met
2.287MetAla: 2.287 ± 0.359
0.26MetCys: 0.26 ± 0.119
1.04MetAsp: 1.04 ± 0.247
0.52MetGlu: 0.52 ± 0.136
0.364MetPhe: 0.364 ± 0.129
1.403MetGly: 1.403 ± 0.276
0.468MetHis: 0.468 ± 0.159
1.092MetIle: 1.092 ± 0.246
0.572MetLys: 0.572 ± 0.197
1.767MetLeu: 1.767 ± 0.277
0.624MetMet: 0.624 ± 0.168
0.884MetAsn: 0.884 ± 0.191
2.339MetPro: 2.339 ± 0.331
0.624MetGln: 0.624 ± 0.216
1.975MetArg: 1.975 ± 0.359
1.299MetSer: 1.299 ± 0.306
3.275MetThr: 3.275 ± 0.396
1.507MetVal: 1.507 ± 0.341
0.468MetTrp: 0.468 ± 0.13
0.416MetTyr: 0.416 ± 0.163
0.0MetXaa: 0.0 ± 0.0
Asn
3.431AsnAla: 3.431 ± 0.496
0.156AsnCys: 0.156 ± 0.079
2.131AsnAsp: 2.131 ± 0.331
1.403AsnGlu: 1.403 ± 0.236
0.468AsnPhe: 0.468 ± 0.162
2.859AsnGly: 2.859 ± 0.366
0.832AsnHis: 0.832 ± 0.232
1.144AsnIle: 1.144 ± 0.333
0.884AsnLys: 0.884 ± 0.199
2.443AsnLeu: 2.443 ± 0.28
0.312AsnMet: 0.312 ± 0.129
0.728AsnAsn: 0.728 ± 0.189
2.235AsnPro: 2.235 ± 0.356
0.676AsnGln: 0.676 ± 0.188
1.611AsnArg: 1.611 ± 0.318
1.611AsnSer: 1.611 ± 0.353
1.351AsnThr: 1.351 ± 0.257
2.235AsnVal: 2.235 ± 0.378
0.676AsnTrp: 0.676 ± 0.197
0.364AsnTyr: 0.364 ± 0.141
0.0AsnXaa: 0.0 ± 0.0
Pro
7.693ProAla: 7.693 ± 0.72
0.676ProCys: 0.676 ± 0.201
6.133ProAsp: 6.133 ± 0.761
3.327ProGlu: 3.327 ± 0.591
1.559ProPhe: 1.559 ± 0.263
5.25ProGly: 5.25 ± 0.567
1.663ProHis: 1.663 ± 0.294
2.287ProIle: 2.287 ± 0.348
1.767ProLys: 1.767 ± 0.319
3.275ProLeu: 3.275 ± 0.48
1.455ProMet: 1.455 ± 0.304
2.027ProAsn: 2.027 ± 0.316
5.146ProPro: 5.146 ± 0.648
1.767ProGln: 1.767 ± 0.253
4.522ProArg: 4.522 ± 0.619
2.235ProSer: 2.235 ± 0.273
4.938ProThr: 4.938 ± 0.667
5.198ProVal: 5.198 ± 0.437
1.611ProTrp: 1.611 ± 0.377
1.455ProTyr: 1.455 ± 0.285
0.0ProXaa: 0.0 ± 0.0
Gln
4.99GlnAla: 4.99 ± 0.514
0.416GlnCys: 0.416 ± 0.143
1.403GlnAsp: 1.403 ± 0.275
1.455GlnGlu: 1.455 ± 0.237
1.144GlnPhe: 1.144 ± 0.234
1.975GlnGly: 1.975 ± 0.426
0.832GlnHis: 0.832 ± 0.24
1.923GlnIle: 1.923 ± 0.341
0.988GlnLys: 0.988 ± 0.24
3.586GlnLeu: 3.586 ± 0.451
1.611GlnMet: 1.611 ± 0.28
0.676GlnAsn: 0.676 ± 0.162
2.027GlnPro: 2.027 ± 0.341
2.547GlnGln: 2.547 ± 0.432
3.638GlnArg: 3.638 ± 0.475
1.611GlnSer: 1.611 ± 0.328
1.975GlnThr: 1.975 ± 0.321
3.223GlnVal: 3.223 ± 0.341
0.988GlnTrp: 0.988 ± 0.23
0.624GlnTyr: 0.624 ± 0.221
0.0GlnXaa: 0.0 ± 0.0
Arg
8.368ArgAla: 8.368 ± 0.816
1.195ArgCys: 1.195 ± 0.299
5.094ArgAsp: 5.094 ± 0.552
4.314ArgGlu: 4.314 ± 0.578
1.299ArgPhe: 1.299 ± 0.289
4.418ArgGly: 4.418 ± 0.541
1.871ArgHis: 1.871 ± 0.357
3.846ArgIle: 3.846 ± 0.4
2.963ArgLys: 2.963 ± 0.425
5.718ArgLeu: 5.718 ± 0.585
2.131ArgMet: 2.131 ± 0.318
1.975ArgAsn: 1.975 ± 0.265
3.638ArgPro: 3.638 ± 0.573
3.379ArgGln: 3.379 ± 0.458
8.161ArgArg: 8.161 ± 0.934
3.015ArgSer: 3.015 ± 0.441
4.314ArgThr: 4.314 ± 0.444
6.029ArgVal: 6.029 ± 0.631
1.559ArgTrp: 1.559 ± 0.319
2.079ArgTyr: 2.079 ± 0.353
0.0ArgXaa: 0.0 ± 0.0
Ser
5.666SerAla: 5.666 ± 0.777
0.364SerCys: 0.364 ± 0.155
2.495SerAsp: 2.495 ± 0.291
1.767SerGlu: 1.767 ± 0.335
0.936SerPhe: 0.936 ± 0.221
4.834SerGly: 4.834 ± 0.52
0.624SerHis: 0.624 ± 0.163
1.923SerIle: 1.923 ± 0.365
1.144SerLys: 1.144 ± 0.28
3.534SerLeu: 3.534 ± 0.356
1.299SerMet: 1.299 ± 0.256
0.884SerAsn: 0.884 ± 0.194
2.443SerPro: 2.443 ± 0.436
1.611SerGln: 1.611 ± 0.337
2.703SerArg: 2.703 ± 0.367
3.171SerSer: 3.171 ± 0.573
3.898SerThr: 3.898 ± 0.462
3.794SerVal: 3.794 ± 0.462
0.988SerTrp: 0.988 ± 0.267
0.936SerTyr: 0.936 ± 0.252
0.0SerXaa: 0.0 ± 0.0
Thr
8.212ThrAla: 8.212 ± 0.93
0.728ThrCys: 0.728 ± 0.198
5.302ThrAsp: 5.302 ± 0.567
4.054ThrGlu: 4.054 ± 0.522
1.975ThrPhe: 1.975 ± 0.31
6.549ThrGly: 6.549 ± 0.786
1.455ThrHis: 1.455 ± 0.282
3.794ThrIle: 3.794 ± 0.469
1.663ThrLys: 1.663 ± 0.299
4.834ThrLeu: 4.834 ± 0.517
1.299ThrMet: 1.299 ± 0.229
1.767ThrAsn: 1.767 ± 0.34
5.718ThrPro: 5.718 ± 0.433
1.715ThrGln: 1.715 ± 0.305
4.418ThrArg: 4.418 ± 0.421
3.534ThrSer: 3.534 ± 0.579
5.042ThrThr: 5.042 ± 0.516
4.626ThrVal: 4.626 ± 0.489
1.195ThrTrp: 1.195 ± 0.213
1.195ThrTyr: 1.195 ± 0.246
0.0ThrXaa: 0.0 ± 0.0
Val
9.2ValAla: 9.2 ± 0.774
0.624ValCys: 0.624 ± 0.22
6.341ValAsp: 6.341 ± 0.561
4.626ValGlu: 4.626 ± 0.502
2.079ValPhe: 2.079 ± 0.314
5.25ValGly: 5.25 ± 0.545
2.131ValHis: 2.131 ± 0.358
3.015ValIle: 3.015 ± 0.36
2.391ValLys: 2.391 ± 0.308
6.185ValLeu: 6.185 ± 0.557
1.403ValMet: 1.403 ± 0.327
1.767ValAsn: 1.767 ± 0.359
4.782ValPro: 4.782 ± 0.504
3.015ValGln: 3.015 ± 0.333
4.938ValArg: 4.938 ± 0.482
3.223ValSer: 3.223 ± 0.52
5.873ValThr: 5.873 ± 0.599
5.977ValVal: 5.977 ± 0.683
1.767ValTrp: 1.767 ± 0.33
2.183ValTyr: 2.183 ± 0.418
0.0ValXaa: 0.0 ± 0.0
Trp
2.287TrpAla: 2.287 ± 0.343
0.416TrpCys: 0.416 ± 0.151
1.247TrpAsp: 1.247 ± 0.242
0.676TrpGlu: 0.676 ± 0.169
0.676TrpPhe: 0.676 ± 0.219
0.988TrpGly: 0.988 ± 0.216
0.78TrpHis: 0.78 ± 0.187
0.624TrpIle: 0.624 ± 0.173
0.468TrpLys: 0.468 ± 0.149
2.287TrpLeu: 2.287 ± 0.308
0.468TrpMet: 0.468 ± 0.17
0.52TrpAsn: 0.52 ± 0.213
1.507TrpPro: 1.507 ± 0.257
1.144TrpGln: 1.144 ± 0.252
1.767TrpArg: 1.767 ± 0.33
1.611TrpSer: 1.611 ± 0.343
1.559TrpThr: 1.559 ± 0.369
1.559TrpVal: 1.559 ± 0.254
0.52TrpTrp: 0.52 ± 0.25
0.208TrpTyr: 0.208 ± 0.097
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.131TyrAla: 2.131 ± 0.315
0.104TyrCys: 0.104 ± 0.089
2.287TyrAsp: 2.287 ± 0.355
1.144TyrGlu: 1.144 ± 0.259
0.676TyrPhe: 0.676 ± 0.148
2.027TyrGly: 2.027 ± 0.281
0.312TyrHis: 0.312 ± 0.116
0.728TyrIle: 0.728 ± 0.17
0.468TyrLys: 0.468 ± 0.155
1.923TyrLeu: 1.923 ± 0.303
0.364TyrMet: 0.364 ± 0.128
0.52TyrAsn: 0.52 ± 0.165
1.247TyrPro: 1.247 ± 0.31
0.936TyrGln: 0.936 ± 0.239
1.819TyrArg: 1.819 ± 0.275
1.04TyrSer: 1.04 ± 0.205
1.403TyrThr: 1.403 ± 0.338
1.351TyrVal: 1.351 ± 0.317
0.52TyrTrp: 0.52 ± 0.143
0.52TyrTyr: 0.52 ± 0.164
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 89 proteins (19240 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski