Thermanaerothrix daxensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Anaerolineae; Anaerolineales; Anaerolineaceae; Thermanaerothrix

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2394 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0P6XRZ9|A0A0P6XRZ9_9CHLR HTH gntR-type domain-containing protein OS=Thermanaerothrix daxensis OX=869279 GN=SE15_08085 PE=4 SV=1
MM1 pKa = 7.56TEE3 pKa = 4.39DD4 pKa = 4.11DD5 pKa = 4.05DD6 pKa = 4.68RR7 pKa = 11.84GYY9 pKa = 10.24MLDD12 pKa = 4.92VFICQQGNLIWWPVALSDD30 pKa = 4.82QYY32 pKa = 9.39QTSYY36 pKa = 10.68TFTDD40 pKa = 3.9EE41 pKa = 4.83PGCSQPSGGVLYY53 pKa = 9.88TVEE56 pKa = 3.91KK57 pKa = 10.14HH58 pKa = 6.21GYY60 pKa = 7.55SHH62 pKa = 7.26PTPIPWPSPP71 pKa = 3.3

Molecular weight: 8.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0P6XK15|A0A0P6XK15_9CHLR Ribose import ATP-binding protein RbsA OS=Thermanaerothrix daxensis OX=869279 GN=rbsA PE=3 SV=1
MM1 pKa = 7.83LSPKK5 pKa = 10.19LRR7 pKa = 11.84TAFALSLLLMGVIPILPPLMLALVKK32 pKa = 10.3RR33 pKa = 11.84LRR35 pKa = 11.84RR36 pKa = 11.84PRR38 pKa = 11.84VRR40 pKa = 11.84VSEE43 pKa = 3.97ATALGRR49 pKa = 11.84RR50 pKa = 11.84GTALLLGPRR59 pKa = 11.84QGRR62 pKa = 11.84SVGRR66 pKa = 3.69

Molecular weight: 7.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2394

0

2394

786151

48

2810

328.4

36.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.072 ± 0.057

0.788 ± 0.015

4.296 ± 0.034

6.487 ± 0.058

3.69 ± 0.035

7.737 ± 0.052

2.149 ± 0.023

5.586 ± 0.045

2.713 ± 0.037

12.18 ± 0.075

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.18 ± 0.021

2.724 ± 0.025

5.867 ± 0.042

4.13 ± 0.039

7.127 ± 0.044

4.779 ± 0.034

5.244 ± 0.037

7.597 ± 0.046

1.731 ± 0.029

2.922 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski