Amino acid dipepetide frequency for Vibrio phage VP-HS15

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.026AlaAla: 8.026 ± 1.738
0.556AlaCys: 0.556 ± 0.214
6.516AlaAsp: 6.516 ± 0.44
5.801AlaGlu: 5.801 ± 0.949
2.702AlaPhe: 2.702 ± 0.501
6.278AlaGly: 6.278 ± 0.591
1.51AlaHis: 1.51 ± 0.384
5.642AlaIle: 5.642 ± 0.69
5.88AlaLys: 5.88 ± 0.775
6.119AlaLeu: 6.119 ± 0.748
2.463AlaMet: 2.463 ± 0.468
4.212AlaAsn: 4.212 ± 0.612
3.02AlaPro: 3.02 ± 0.517
3.338AlaGln: 3.338 ± 0.464
4.847AlaArg: 4.847 ± 0.727
3.497AlaSer: 3.497 ± 0.631
3.417AlaThr: 3.417 ± 0.566
4.688AlaVal: 4.688 ± 0.573
0.795AlaTrp: 0.795 ± 0.303
3.099AlaTyr: 3.099 ± 0.552
0.0AlaXaa: 0.0 ± 0.0
Cys
0.238CysAla: 0.238 ± 0.121
0.0CysCys: 0.0 ± 0.0
0.397CysAsp: 0.397 ± 0.211
0.795CysGlu: 0.795 ± 0.343
0.238CysPhe: 0.238 ± 0.115
0.874CysGly: 0.874 ± 0.345
0.318CysHis: 0.318 ± 0.148
0.636CysIle: 0.636 ± 0.216
0.636CysLys: 0.636 ± 0.208
0.954CysLeu: 0.954 ± 0.355
0.159CysMet: 0.159 ± 0.106
0.477CysAsn: 0.477 ± 0.182
0.556CysPro: 0.556 ± 0.21
0.477CysGln: 0.477 ± 0.158
0.954CysArg: 0.954 ± 0.398
0.238CysSer: 0.238 ± 0.137
0.477CysThr: 0.477 ± 0.152
0.795CysVal: 0.795 ± 0.218
0.238CysTrp: 0.238 ± 0.12
0.397CysTyr: 0.397 ± 0.153
0.0CysXaa: 0.0 ± 0.0
Asp
5.324AspAla: 5.324 ± 0.664
0.477AspCys: 0.477 ± 0.16
4.291AspAsp: 4.291 ± 0.635
4.688AspGlu: 4.688 ± 0.691
3.497AspPhe: 3.497 ± 0.548
5.563AspGly: 5.563 ± 0.814
0.954AspHis: 0.954 ± 0.254
4.053AspIle: 4.053 ± 0.711
5.324AspLys: 5.324 ± 0.774
4.688AspLeu: 4.688 ± 0.55
1.51AspMet: 1.51 ± 0.39
3.338AspAsn: 3.338 ± 0.536
2.622AspPro: 2.622 ± 0.455
1.351AspGln: 1.351 ± 0.383
2.543AspArg: 2.543 ± 0.437
4.053AspSer: 4.053 ± 0.521
3.417AspThr: 3.417 ± 0.489
4.768AspVal: 4.768 ± 0.569
1.192AspTrp: 1.192 ± 0.422
3.02AspTyr: 3.02 ± 0.388
0.0AspXaa: 0.0 ± 0.0
Glu
6.119GluAla: 6.119 ± 0.977
0.636GluCys: 0.636 ± 0.23
4.609GluAsp: 4.609 ± 0.577
7.549GluGlu: 7.549 ± 1.138
3.894GluPhe: 3.894 ± 0.345
4.847GluGly: 4.847 ± 0.679
1.51GluHis: 1.51 ± 0.442
3.179GluIle: 3.179 ± 0.314
4.927GluLys: 4.927 ± 0.635
6.675GluLeu: 6.675 ± 0.907
2.305GluMet: 2.305 ± 0.53
2.781GluAsn: 2.781 ± 0.361
2.861GluPro: 2.861 ± 0.637
4.688GluGln: 4.688 ± 0.727
3.417GluArg: 3.417 ± 0.443
4.53GluSer: 4.53 ± 0.745
3.099GluThr: 3.099 ± 0.364
4.45GluVal: 4.45 ± 0.722
1.828GluTrp: 1.828 ± 0.417
2.702GluTyr: 2.702 ± 0.418
0.0GluXaa: 0.0 ± 0.0
Phe
3.02PheAla: 3.02 ± 0.31
0.318PheCys: 0.318 ± 0.19
3.497PheAsp: 3.497 ± 0.675
2.622PheGlu: 2.622 ± 0.387
1.589PhePhe: 1.589 ± 0.296
3.02PheGly: 3.02 ± 0.512
0.874PheHis: 0.874 ± 0.193
2.066PheIle: 2.066 ± 0.36
2.384PheLys: 2.384 ± 0.451
2.702PheLeu: 2.702 ± 0.424
1.669PheMet: 1.669 ± 0.422
1.828PheAsn: 1.828 ± 0.261
1.43PhePro: 1.43 ± 0.346
1.033PheGln: 1.033 ± 0.246
2.781PheArg: 2.781 ± 0.46
2.702PheSer: 2.702 ± 0.527
1.907PheThr: 1.907 ± 0.36
2.146PheVal: 2.146 ± 0.481
0.636PheTrp: 0.636 ± 0.263
1.271PheTyr: 1.271 ± 0.308
0.0PheXaa: 0.0 ± 0.0
Gly
5.245GlyAla: 5.245 ± 0.705
1.033GlyCys: 1.033 ± 0.297
4.609GlyAsp: 4.609 ± 0.648
6.039GlyGlu: 6.039 ± 0.655
2.702GlyPhe: 2.702 ± 0.438
5.642GlyGly: 5.642 ± 0.93
1.669GlyHis: 1.669 ± 0.417
3.735GlyIle: 3.735 ± 0.536
6.834GlyLys: 6.834 ± 0.905
5.88GlyLeu: 5.88 ± 0.76
1.907GlyMet: 1.907 ± 0.359
3.973GlyAsn: 3.973 ± 0.564
0.238GlyPro: 0.238 ± 0.189
2.861GlyGln: 2.861 ± 0.529
3.576GlyArg: 3.576 ± 0.435
4.609GlySer: 4.609 ± 0.731
4.927GlyThr: 4.927 ± 0.866
3.417GlyVal: 3.417 ± 0.713
0.954GlyTrp: 0.954 ± 0.274
3.258GlyTyr: 3.258 ± 0.425
0.0GlyXaa: 0.0 ± 0.0
His
1.271HisAla: 1.271 ± 0.419
0.238HisCys: 0.238 ± 0.167
1.351HisAsp: 1.351 ± 0.334
1.192HisGlu: 1.192 ± 0.281
0.715HisPhe: 0.715 ± 0.198
1.033HisGly: 1.033 ± 0.304
0.556HisHis: 0.556 ± 0.214
1.351HisIle: 1.351 ± 0.368
1.192HisLys: 1.192 ± 0.265
2.305HisLeu: 2.305 ± 0.512
0.397HisMet: 0.397 ± 0.155
0.715HisAsn: 0.715 ± 0.217
0.715HisPro: 0.715 ± 0.268
1.113HisGln: 1.113 ± 0.285
0.795HisArg: 0.795 ± 0.215
1.192HisSer: 1.192 ± 0.26
0.795HisThr: 0.795 ± 0.279
0.715HisVal: 0.715 ± 0.302
0.159HisTrp: 0.159 ± 0.111
0.795HisTyr: 0.795 ± 0.307
0.0HisXaa: 0.0 ± 0.0
Ile
4.847IleAla: 4.847 ± 0.545
0.318IleCys: 0.318 ± 0.151
5.165IleAsp: 5.165 ± 0.641
3.735IleGlu: 3.735 ± 0.564
1.589IlePhe: 1.589 ± 0.407
4.053IleGly: 4.053 ± 0.566
1.113IleHis: 1.113 ± 0.311
2.622IleIle: 2.622 ± 0.624
4.291IleLys: 4.291 ± 0.637
3.417IleLeu: 3.417 ± 0.374
1.589IleMet: 1.589 ± 0.309
2.94IleAsn: 2.94 ± 0.606
1.987IlePro: 1.987 ± 0.344
2.543IleGln: 2.543 ± 0.536
3.02IleArg: 3.02 ± 0.516
2.622IleSer: 2.622 ± 0.47
3.02IleThr: 3.02 ± 0.494
2.384IleVal: 2.384 ± 0.542
0.795IleTrp: 0.795 ± 0.228
2.225IleTyr: 2.225 ± 0.542
0.0IleXaa: 0.0 ± 0.0
Lys
6.516LysAla: 6.516 ± 0.835
0.636LysCys: 0.636 ± 0.248
5.165LysAsp: 5.165 ± 0.763
5.801LysGlu: 5.801 ± 0.877
2.384LysPhe: 2.384 ± 0.482
3.973LysGly: 3.973 ± 0.817
1.43LysHis: 1.43 ± 0.257
2.543LysIle: 2.543 ± 0.327
3.338LysLys: 3.338 ± 0.601
5.165LysLeu: 5.165 ± 0.653
1.43LysMet: 1.43 ± 0.32
2.702LysAsn: 2.702 ± 0.421
3.338LysPro: 3.338 ± 0.644
3.417LysGln: 3.417 ± 0.448
4.371LysArg: 4.371 ± 0.576
3.099LysSer: 3.099 ± 0.459
3.814LysThr: 3.814 ± 0.387
3.814LysVal: 3.814 ± 0.726
0.636LysTrp: 0.636 ± 0.173
2.861LysTyr: 2.861 ± 0.382
0.0LysXaa: 0.0 ± 0.0
Leu
5.642LeuAla: 5.642 ± 0.756
0.556LeuCys: 0.556 ± 0.222
5.086LeuAsp: 5.086 ± 0.582
6.993LeuGlu: 6.993 ± 0.593
2.146LeuPhe: 2.146 ± 0.289
5.563LeuGly: 5.563 ± 0.45
1.748LeuHis: 1.748 ± 0.422
4.847LeuIle: 4.847 ± 0.563
5.88LeuLys: 5.88 ± 0.707
6.516LeuLeu: 6.516 ± 0.892
2.702LeuMet: 2.702 ± 0.358
3.02LeuAsn: 3.02 ± 0.508
3.655LeuPro: 3.655 ± 0.651
3.02LeuGln: 3.02 ± 0.486
4.768LeuArg: 4.768 ± 0.745
5.006LeuSer: 5.006 ± 0.852
5.086LeuThr: 5.086 ± 0.609
6.119LeuVal: 6.119 ± 0.588
0.795LeuTrp: 0.795 ± 0.236
2.622LeuTyr: 2.622 ± 0.36
0.0LeuXaa: 0.0 ± 0.0
Met
2.861MetAla: 2.861 ± 0.385
0.079MetCys: 0.079 ± 0.075
1.271MetAsp: 1.271 ± 0.335
1.589MetGlu: 1.589 ± 0.509
0.874MetPhe: 0.874 ± 0.28
1.748MetGly: 1.748 ± 0.45
0.397MetHis: 0.397 ± 0.175
1.033MetIle: 1.033 ± 0.271
2.305MetLys: 2.305 ± 0.365
3.258MetLeu: 3.258 ± 0.418
0.477MetMet: 0.477 ± 0.207
1.113MetAsn: 1.113 ± 0.314
1.113MetPro: 1.113 ± 0.334
1.748MetGln: 1.748 ± 0.363
1.669MetArg: 1.669 ± 0.312
2.384MetSer: 2.384 ± 0.467
1.51MetThr: 1.51 ± 0.429
1.192MetVal: 1.192 ± 0.256
0.079MetTrp: 0.079 ± 0.067
1.033MetTyr: 1.033 ± 0.326
0.0MetXaa: 0.0 ± 0.0
Asn
4.371AsnAla: 4.371 ± 0.412
0.477AsnCys: 0.477 ± 0.193
3.417AsnAsp: 3.417 ± 0.458
2.543AsnGlu: 2.543 ± 0.363
1.748AsnPhe: 1.748 ± 0.262
3.814AsnGly: 3.814 ± 0.744
0.397AsnHis: 0.397 ± 0.162
2.384AsnIle: 2.384 ± 0.279
3.497AsnLys: 3.497 ± 0.654
5.404AsnLeu: 5.404 ± 0.544
1.033AsnMet: 1.033 ± 0.28
3.179AsnAsn: 3.179 ± 0.587
2.463AsnPro: 2.463 ± 0.445
2.305AsnGln: 2.305 ± 0.378
1.987AsnArg: 1.987 ± 0.373
2.94AsnSer: 2.94 ± 0.377
2.622AsnThr: 2.622 ± 0.43
3.417AsnVal: 3.417 ± 0.523
0.477AsnTrp: 0.477 ± 0.182
1.271AsnTyr: 1.271 ± 0.316
0.0AsnXaa: 0.0 ± 0.0
Pro
3.417ProAla: 3.417 ± 0.453
0.636ProCys: 0.636 ± 0.249
2.384ProAsp: 2.384 ± 0.383
4.132ProGlu: 4.132 ± 0.64
1.351ProPhe: 1.351 ± 0.366
0.477ProGly: 0.477 ± 0.304
0.397ProHis: 0.397 ± 0.143
2.225ProIle: 2.225 ± 0.521
1.748ProLys: 1.748 ± 0.336
3.02ProLeu: 3.02 ± 0.372
1.033ProMet: 1.033 ± 0.22
2.543ProAsn: 2.543 ± 0.35
1.192ProPro: 1.192 ± 0.33
1.748ProGln: 1.748 ± 0.28
1.033ProArg: 1.033 ± 0.396
2.384ProSer: 2.384 ± 0.403
1.669ProThr: 1.669 ± 0.301
2.94ProVal: 2.94 ± 0.573
0.636ProTrp: 0.636 ± 0.194
1.43ProTyr: 1.43 ± 0.295
0.0ProXaa: 0.0 ± 0.0
Gln
4.53GlnAla: 4.53 ± 0.592
0.477GlnCys: 0.477 ± 0.149
1.589GlnAsp: 1.589 ± 0.308
4.212GlnGlu: 4.212 ± 0.709
2.066GlnPhe: 2.066 ± 0.347
3.179GlnGly: 3.179 ± 0.528
0.874GlnHis: 0.874 ± 0.218
2.225GlnIle: 2.225 ± 0.29
1.669GlnLys: 1.669 ± 0.42
3.497GlnLeu: 3.497 ± 0.573
1.351GlnMet: 1.351 ± 0.303
2.463GlnAsn: 2.463 ± 0.333
1.271GlnPro: 1.271 ± 0.333
2.94GlnGln: 2.94 ± 0.58
3.258GlnArg: 3.258 ± 0.632
1.828GlnSer: 1.828 ± 0.352
1.987GlnThr: 1.987 ± 0.417
2.543GlnVal: 2.543 ± 0.444
0.318GlnTrp: 0.318 ± 0.186
1.192GlnTyr: 1.192 ± 0.277
0.0GlnXaa: 0.0 ± 0.0
Arg
4.053ArgAla: 4.053 ± 0.711
0.556ArgCys: 0.556 ± 0.201
3.179ArgAsp: 3.179 ± 0.392
3.894ArgGlu: 3.894 ± 0.563
1.907ArgPhe: 1.907 ± 0.372
3.894ArgGly: 3.894 ± 0.521
0.954ArgHis: 0.954 ± 0.272
3.02ArgIle: 3.02 ± 0.413
3.258ArgLys: 3.258 ± 0.527
4.291ArgLeu: 4.291 ± 0.553
1.589ArgMet: 1.589 ± 0.25
2.066ArgAsn: 2.066 ± 0.42
1.351ArgPro: 1.351 ± 0.339
1.907ArgGln: 1.907 ± 0.311
2.94ArgArg: 2.94 ± 0.524
2.622ArgSer: 2.622 ± 0.358
3.02ArgThr: 3.02 ± 0.322
3.814ArgVal: 3.814 ± 0.531
1.192ArgTrp: 1.192 ± 0.236
2.305ArgTyr: 2.305 ± 0.498
0.0ArgXaa: 0.0 ± 0.0
Ser
4.291SerAla: 4.291 ± 0.716
0.556SerCys: 0.556 ± 0.164
3.576SerAsp: 3.576 ± 0.514
3.735SerGlu: 3.735 ± 0.557
2.463SerPhe: 2.463 ± 0.442
5.165SerGly: 5.165 ± 0.648
1.113SerHis: 1.113 ± 0.272
4.053SerIle: 4.053 ± 0.496
3.814SerLys: 3.814 ± 0.536
5.801SerLeu: 5.801 ± 0.794
2.146SerMet: 2.146 ± 0.516
2.94SerAsn: 2.94 ± 0.469
1.828SerPro: 1.828 ± 0.383
1.907SerGln: 1.907 ± 0.299
2.463SerArg: 2.463 ± 0.386
3.258SerSer: 3.258 ± 0.596
2.781SerThr: 2.781 ± 0.482
3.179SerVal: 3.179 ± 0.565
0.715SerTrp: 0.715 ± 0.198
1.51SerTyr: 1.51 ± 0.431
0.0SerXaa: 0.0 ± 0.0
Thr
3.497ThrAla: 3.497 ± 0.434
0.715ThrCys: 0.715 ± 0.2
3.735ThrAsp: 3.735 ± 0.562
3.973ThrGlu: 3.973 ± 0.601
3.417ThrPhe: 3.417 ± 0.548
4.768ThrGly: 4.768 ± 0.611
0.954ThrHis: 0.954 ± 0.235
2.622ThrIle: 2.622 ± 0.47
2.702ThrLys: 2.702 ± 0.375
4.609ThrLeu: 4.609 ± 0.558
1.113ThrMet: 1.113 ± 0.318
2.543ThrAsn: 2.543 ± 0.522
2.384ThrPro: 2.384 ± 0.506
2.384ThrGln: 2.384 ± 0.479
1.987ThrArg: 1.987 ± 0.376
2.94ThrSer: 2.94 ± 0.683
3.258ThrThr: 3.258 ± 0.567
4.053ThrVal: 4.053 ± 0.696
0.477ThrTrp: 0.477 ± 0.192
1.43ThrTyr: 1.43 ± 0.337
0.0ThrXaa: 0.0 ± 0.0
Val
5.642ValAla: 5.642 ± 0.627
0.636ValCys: 0.636 ± 0.252
4.132ValAsp: 4.132 ± 0.587
3.576ValGlu: 3.576 ± 0.643
2.384ValPhe: 2.384 ± 0.546
5.563ValGly: 5.563 ± 0.419
0.795ValHis: 0.795 ± 0.25
3.338ValIle: 3.338 ± 0.47
3.417ValLys: 3.417 ± 0.434
3.973ValLeu: 3.973 ± 0.581
1.51ValMet: 1.51 ± 0.323
3.894ValAsn: 3.894 ± 0.49
2.066ValPro: 2.066 ± 0.356
2.463ValGln: 2.463 ± 0.451
3.02ValArg: 3.02 ± 0.521
4.132ValSer: 4.132 ± 0.923
3.735ValThr: 3.735 ± 0.897
4.847ValVal: 4.847 ± 0.661
0.795ValTrp: 0.795 ± 0.276
1.351ValTyr: 1.351 ± 0.518
0.0ValXaa: 0.0 ± 0.0
Trp
0.954TrpAla: 0.954 ± 0.284
0.397TrpCys: 0.397 ± 0.199
0.477TrpAsp: 0.477 ± 0.138
1.113TrpGlu: 1.113 ± 0.356
0.795TrpPhe: 0.795 ± 0.278
0.954TrpGly: 0.954 ± 0.24
0.318TrpHis: 0.318 ± 0.194
0.636TrpIle: 0.636 ± 0.234
1.271TrpLys: 1.271 ± 0.332
1.271TrpLeu: 1.271 ± 0.375
0.477TrpMet: 0.477 ± 0.175
0.477TrpAsn: 0.477 ± 0.158
0.397TrpPro: 0.397 ± 0.207
0.636TrpGln: 0.636 ± 0.239
0.318TrpArg: 0.318 ± 0.195
1.192TrpSer: 1.192 ± 0.307
0.636TrpThr: 0.636 ± 0.209
0.477TrpVal: 0.477 ± 0.167
0.159TrpTrp: 0.159 ± 0.117
0.795TrpTyr: 0.795 ± 0.156
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.543TyrAla: 2.543 ± 0.45
0.556TyrCys: 0.556 ± 0.18
2.225TyrAsp: 2.225 ± 0.39
2.622TyrGlu: 2.622 ± 0.393
1.033TyrPhe: 1.033 ± 0.246
2.702TyrGly: 2.702 ± 0.357
0.715TyrHis: 0.715 ± 0.26
2.066TyrIle: 2.066 ± 0.419
2.066TyrLys: 2.066 ± 0.366
2.225TyrLeu: 2.225 ± 0.345
0.795TyrMet: 0.795 ± 0.213
2.543TyrAsn: 2.543 ± 0.365
1.907TyrPro: 1.907 ± 0.462
1.589TyrGln: 1.589 ± 0.227
2.146TyrArg: 2.146 ± 0.29
2.066TyrSer: 2.066 ± 0.495
2.384TyrThr: 2.384 ± 0.402
1.51TyrVal: 1.51 ± 0.308
0.874TyrTrp: 0.874 ± 0.265
1.192TyrTyr: 1.192 ± 0.31
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 30 proteins (12585 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski