Amino acid dipepetide frequency for Parcubacteria bacterium RAAC4_OD1_1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.628AlaAla: 1.628 ± 0.125
0.44AlaCys: 0.44 ± 0.054
2.3AlaAsp: 2.3 ± 0.136
2.981AlaGlu: 2.981 ± 0.124
2.203AlaPhe: 2.203 ± 0.1
3.532AlaGly: 3.532 ± 0.217
0.865AlaHis: 0.865 ± 0.061
5.199AlaIle: 5.199 ± 0.177
4.339AlaLys: 4.339 ± 0.161
4.977AlaLeu: 4.977 ± 0.164
1.237AlaMet: 1.237 ± 0.082
2.58AlaAsn: 2.58 ± 0.128
1.532AlaPro: 1.532 ± 0.094
1.305AlaGln: 1.305 ± 0.071
1.865AlaArg: 1.865 ± 0.115
3.9AlaSer: 3.9 ± 0.159
2.943AlaThr: 2.943 ± 0.225
3.165AlaVal: 3.165 ± 0.13
0.464AlaTrp: 0.464 ± 0.04
1.981AlaTyr: 1.981 ± 0.104
0.0AlaXaa: 0.0 ± 0.0
Cys
0.416CysAla: 0.416 ± 0.051
0.034CysCys: 0.034 ± 0.014
0.488CysAsp: 0.488 ± 0.05
0.575CysGlu: 0.575 ± 0.054
0.329CysPhe: 0.329 ± 0.047
0.754CysGly: 0.754 ± 0.073
0.14CysHis: 0.14 ± 0.035
0.546CysIle: 0.546 ± 0.063
0.619CysLys: 0.619 ± 0.058
0.536CysLeu: 0.536 ± 0.054
0.188CysMet: 0.188 ± 0.027
0.42CysAsn: 0.42 ± 0.051
0.44CysPro: 0.44 ± 0.051
0.227CysGln: 0.227 ± 0.037
0.275CysArg: 0.275 ± 0.051
0.57CysSer: 0.57 ± 0.053
0.338CysThr: 0.338 ± 0.056
0.43CysVal: 0.43 ± 0.055
0.058CysTrp: 0.058 ± 0.017
0.348CysTyr: 0.348 ± 0.063
0.0CysXaa: 0.0 ± 0.0
Asp
2.585AspAla: 2.585 ± 0.135
0.57AspCys: 0.57 ± 0.075
2.542AspAsp: 2.542 ± 0.139
4.059AspGlu: 4.059 ± 0.173
3.035AspPhe: 3.035 ± 0.123
3.914AspGly: 3.914 ± 0.186
0.734AspHis: 0.734 ± 0.064
5.629AspIle: 5.629 ± 0.159
5.088AspLys: 5.088 ± 0.196
4.943AspLeu: 4.943 ± 0.158
1.329AspMet: 1.329 ± 0.084
3.262AspAsn: 3.262 ± 0.16
1.996AspPro: 1.996 ± 0.102
1.194AspGln: 1.194 ± 0.09
1.957AspArg: 1.957 ± 0.107
3.397AspSer: 3.397 ± 0.162
2.88AspThr: 2.88 ± 0.121
3.175AspVal: 3.175 ± 0.132
0.619AspTrp: 0.619 ± 0.048
2.237AspTyr: 2.237 ± 0.116
0.005AspXaa: 0.005 ± 0.004
Glu
3.846GluAla: 3.846 ± 0.154
0.512GluCys: 0.512 ± 0.06
4.257GluAsp: 4.257 ± 0.188
7.253GluGlu: 7.253 ± 0.269
2.827GluPhe: 2.827 ± 0.127
3.938GluGly: 3.938 ± 0.162
0.894GluHis: 0.894 ± 0.074
7.872GluIle: 7.872 ± 0.246
8.814GluLys: 8.814 ± 0.322
5.61GluLeu: 5.61 ± 0.229
1.744GluMet: 1.744 ± 0.107
5.673GluAsn: 5.673 ± 0.196
1.45GluPro: 1.45 ± 0.084
1.566GluGln: 1.566 ± 0.091
2.856GluArg: 2.856 ± 0.128
3.948GluSer: 3.948 ± 0.15
3.469GluThr: 3.469 ± 0.143
4.402GluVal: 4.402 ± 0.148
0.536GluTrp: 0.536 ± 0.052
2.634GluTyr: 2.634 ± 0.135
0.0GluXaa: 0.0 ± 0.0
Phe
2.353PheAla: 2.353 ± 0.117
0.435PheCys: 0.435 ± 0.053
2.783PheAsp: 2.783 ± 0.131
3.02PheGlu: 3.02 ± 0.124
2.682PhePhe: 2.682 ± 0.135
3.257PheGly: 3.257 ± 0.152
0.551PheHis: 0.551 ± 0.056
5.446PheIle: 5.446 ± 0.219
3.89PheLys: 3.89 ± 0.163
4.421PheLeu: 4.421 ± 0.174
0.918PheMet: 0.918 ± 0.076
2.764PheAsn: 2.764 ± 0.129
1.527PhePro: 1.527 ± 0.091
0.991PheGln: 0.991 ± 0.065
1.387PheArg: 1.387 ± 0.078
4.006PheSer: 4.006 ± 0.138
2.402PheThr: 2.402 ± 0.113
3.107PheVal: 3.107 ± 0.123
0.483PheTrp: 0.483 ± 0.049
1.894PheTyr: 1.894 ± 0.105
0.0PheXaa: 0.0 ± 0.0
Gly
3.866GlyAla: 3.866 ± 0.201
0.488GlyCys: 0.488 ± 0.059
3.832GlyAsp: 3.832 ± 0.146
4.905GlyGlu: 4.905 ± 0.178
3.117GlyPhe: 3.117 ± 0.15
5.6GlyGly: 5.6 ± 0.343
1.097GlyHis: 1.097 ± 0.072
6.175GlyIle: 6.175 ± 0.19
5.451GlyLys: 5.451 ± 0.2
5.426GlyLeu: 5.426 ± 0.179
1.585GlyMet: 1.585 ± 0.086
3.46GlyAsn: 3.46 ± 0.185
1.479GlyPro: 1.479 ± 0.084
1.57GlyGln: 1.57 ± 0.088
2.547GlyArg: 2.547 ± 0.097
4.446GlySer: 4.446 ± 0.208
3.924GlyThr: 3.924 ± 0.444
4.934GlyVal: 4.934 ± 0.148
0.667GlyTrp: 0.667 ± 0.053
2.798GlyTyr: 2.798 ± 0.142
0.0GlyXaa: 0.0 ± 0.0
His
0.575HisAla: 0.575 ± 0.057
0.116HisCys: 0.116 ± 0.022
0.614HisAsp: 0.614 ± 0.057
0.865HisGlu: 0.865 ± 0.079
0.763HisPhe: 0.763 ± 0.065
0.889HisGly: 0.889 ± 0.081
0.343HisHis: 0.343 ± 0.044
1.624HisIle: 1.624 ± 0.097
1.194HisLys: 1.194 ± 0.088
1.396HisLeu: 1.396 ± 0.093
0.42HisMet: 0.42 ± 0.042
0.831HisAsn: 0.831 ± 0.055
0.667HisPro: 0.667 ± 0.06
0.469HisGln: 0.469 ± 0.048
0.594HisArg: 0.594 ± 0.051
0.981HisSer: 0.981 ± 0.066
0.884HisThr: 0.884 ± 0.072
0.488HisVal: 0.488 ± 0.044
0.13HisTrp: 0.13 ± 0.025
0.546HisTyr: 0.546 ± 0.054
0.0HisXaa: 0.0 ± 0.0
Ile
4.789IleAla: 4.789 ± 0.165
0.662IleCys: 0.662 ± 0.062
5.755IleAsp: 5.755 ± 0.174
7.045IleGlu: 7.045 ± 0.219
5.059IlePhe: 5.059 ± 0.221
5.895IleGly: 5.895 ± 0.177
1.387IleHis: 1.387 ± 0.077
10.003IleIle: 10.003 ± 0.308
10.143IleLys: 10.143 ± 0.328
9.481IleLeu: 9.481 ± 0.25
1.798IleMet: 1.798 ± 0.104
6.171IleAsn: 6.171 ± 0.194
3.784IlePro: 3.784 ± 0.137
1.889IleGln: 1.889 ± 0.092
3.059IleArg: 3.059 ± 0.127
7.214IleSer: 7.214 ± 0.19
5.074IleThr: 5.074 ± 0.152
5.542IleVal: 5.542 ± 0.182
0.768IleTrp: 0.768 ± 0.071
3.276IleTyr: 3.276 ± 0.123
0.0IleXaa: 0.0 ± 0.0
Lys
4.059LysAla: 4.059 ± 0.131
0.59LysCys: 0.59 ± 0.062
6.543LysAsp: 6.543 ± 0.226
10.133LysGlu: 10.133 ± 0.344
3.194LysPhe: 3.194 ± 0.109
4.861LysGly: 4.861 ± 0.167
1.126LysHis: 1.126 ± 0.087
10.626LysIle: 10.626 ± 0.332
10.452LysLys: 10.452 ± 0.37
6.775LysLeu: 6.775 ± 0.241
2.663LysMet: 2.663 ± 0.122
7.64LysAsn: 7.64 ± 0.235
2.136LysPro: 2.136 ± 0.103
2.116LysGln: 2.116 ± 0.105
3.136LysArg: 3.136 ± 0.144
5.219LysSer: 5.219 ± 0.171
4.75LysThr: 4.75 ± 0.16
5.011LysVal: 5.011 ± 0.188
0.739LysTrp: 0.739 ± 0.063
3.658LysTyr: 3.658 ± 0.149
0.005LysXaa: 0.005 ± 0.005
Leu
4.566LeuAla: 4.566 ± 0.144
0.507LeuCys: 0.507 ± 0.055
4.595LeuAsp: 4.595 ± 0.15
6.47LeuGlu: 6.47 ± 0.231
4.552LeuPhe: 4.552 ± 0.183
6.311LeuGly: 6.311 ± 0.189
1.227LeuHis: 1.227 ± 0.081
7.34LeuIle: 7.34 ± 0.25
8.128LeuLys: 8.128 ± 0.229
7.021LeuLeu: 7.021 ± 0.213
1.87LeuMet: 1.87 ± 0.095
5.282LeuAsn: 5.282 ± 0.174
3.247LeuPro: 3.247 ± 0.137
1.633LeuGln: 1.633 ± 0.1
3.136LeuArg: 3.136 ± 0.135
7.432LeuSer: 7.432 ± 0.175
4.006LeuThr: 4.006 ± 0.172
5.484LeuVal: 5.484 ± 0.15
0.715LeuTrp: 0.715 ± 0.062
2.933LeuTyr: 2.933 ± 0.123
0.0LeuXaa: 0.0 ± 0.0
Met
1.237MetAla: 1.237 ± 0.096
0.169MetCys: 0.169 ± 0.031
1.111MetAsp: 1.111 ± 0.081
1.609MetGlu: 1.609 ± 0.098
1.024MetPhe: 1.024 ± 0.07
1.459MetGly: 1.459 ± 0.083
0.353MetHis: 0.353 ± 0.043
2.112MetIle: 2.112 ± 0.096
2.281MetLys: 2.281 ± 0.12
1.764MetLeu: 1.764 ± 0.104
0.58MetMet: 0.58 ± 0.058
1.31MetAsn: 1.31 ± 0.077
0.749MetPro: 0.749 ± 0.055
0.57MetGln: 0.57 ± 0.054
0.826MetArg: 0.826 ± 0.059
1.72MetSer: 1.72 ± 0.105
1.213MetThr: 1.213 ± 0.078
1.223MetVal: 1.223 ± 0.069
0.179MetTrp: 0.179 ± 0.028
0.725MetTyr: 0.725 ± 0.049
0.0MetXaa: 0.0 ± 0.0
Asn
2.841AsnAla: 2.841 ± 0.132
0.551AsnCys: 0.551 ± 0.101
3.025AsnAsp: 3.025 ± 0.124
4.296AsnGlu: 4.296 ± 0.195
2.952AsnPhe: 2.952 ± 0.131
3.822AsnGly: 3.822 ± 0.193
0.836AsnHis: 0.836 ± 0.06
6.649AsnIle: 6.649 ± 0.221
6.789AsnLys: 6.789 ± 0.216
5.301AsnLeu: 5.301 ± 0.176
1.237AsnMet: 1.237 ± 0.083
4.504AsnAsn: 4.504 ± 0.202
2.464AsnPro: 2.464 ± 0.107
1.537AsnGln: 1.537 ± 0.099
1.943AsnArg: 1.943 ± 0.109
3.943AsnSer: 3.943 ± 0.189
3.527AsnThr: 3.527 ± 0.211
3.097AsnVal: 3.097 ± 0.121
0.662AsnTrp: 0.662 ± 0.066
2.261AsnTyr: 2.261 ± 0.109
0.0AsnXaa: 0.0 ± 0.0
Pro
1.411ProAla: 1.411 ± 0.072
0.227ProCys: 0.227 ± 0.04
1.986ProAsp: 1.986 ± 0.102
2.899ProGlu: 2.899 ± 0.138
1.773ProPhe: 1.773 ± 0.102
1.643ProGly: 1.643 ± 0.084
0.503ProHis: 0.503 ± 0.054
3.001ProIle: 3.001 ± 0.118
2.783ProLys: 2.783 ± 0.125
2.614ProLeu: 2.614 ± 0.105
0.744ProMet: 0.744 ± 0.066
2.029ProAsn: 2.029 ± 0.099
0.865ProPro: 0.865 ± 0.066
0.783ProGln: 0.783 ± 0.061
1.097ProArg: 1.097 ± 0.081
2.165ProSer: 2.165 ± 0.106
1.923ProThr: 1.923 ± 0.145
2.087ProVal: 2.087 ± 0.105
0.28ProTrp: 0.28 ± 0.042
1.396ProTyr: 1.396 ± 0.08
0.0ProXaa: 0.0 ± 0.0
Gln
1.305GlnAla: 1.305 ± 0.078
0.116GlnCys: 0.116 ± 0.028
1.165GlnAsp: 1.165 ± 0.078
1.599GlnGlu: 1.599 ± 0.099
0.933GlnPhe: 0.933 ± 0.075
1.305GlnGly: 1.305 ± 0.086
0.213GlnHis: 0.213 ± 0.031
2.474GlnIle: 2.474 ± 0.104
2.576GlnLys: 2.576 ± 0.111
1.503GlnLeu: 1.503 ± 0.087
0.536GlnMet: 0.536 ± 0.06
1.653GlnAsn: 1.653 ± 0.107
0.648GlnPro: 0.648 ± 0.057
0.445GlnGln: 0.445 ± 0.07
0.648GlnArg: 0.648 ± 0.067
1.454GlnSer: 1.454 ± 0.087
1.295GlnThr: 1.295 ± 0.088
1.416GlnVal: 1.416 ± 0.084
0.169GlnTrp: 0.169 ± 0.029
0.725GlnTyr: 0.725 ± 0.064
0.0GlnXaa: 0.0 ± 0.0
Arg
1.764ArgAla: 1.764 ± 0.098
0.217ArgCys: 0.217 ± 0.032
2.039ArgAsp: 2.039 ± 0.111
2.566ArgGlu: 2.566 ± 0.113
1.493ArgPhe: 1.493 ± 0.088
2.45ArgGly: 2.45 ± 0.118
0.575ArgHis: 0.575 ± 0.054
3.078ArgIle: 3.078 ± 0.12
2.962ArgLys: 2.962 ± 0.141
3.078ArgLeu: 3.078 ± 0.138
0.981ArgMet: 0.981 ± 0.083
2.063ArgAsn: 2.063 ± 0.116
1.116ArgPro: 1.116 ± 0.079
0.904ArgGln: 0.904 ± 0.065
1.469ArgArg: 1.469 ± 0.091
1.933ArgSer: 1.933 ± 0.092
1.657ArgThr: 1.657 ± 0.086
2.455ArgVal: 2.455 ± 0.111
0.275ArgTrp: 0.275 ± 0.037
1.281ArgTyr: 1.281 ± 0.069
0.0ArgXaa: 0.0 ± 0.0
Ser
3.344SerAla: 3.344 ± 0.183
0.604SerCys: 0.604 ± 0.067
3.426SerAsp: 3.426 ± 0.136
3.837SerGlu: 3.837 ± 0.13
4.059SerPhe: 4.059 ± 0.146
5.306SerGly: 5.306 ± 0.237
1.145SerHis: 1.145 ± 0.074
6.601SerIle: 6.601 ± 0.208
6.369SerLys: 6.369 ± 0.206
6.857SerLeu: 6.857 ± 0.211
1.387SerMet: 1.387 ± 0.083
3.813SerAsn: 3.813 ± 0.189
2.116SerPro: 2.116 ± 0.108
1.609SerGln: 1.609 ± 0.09
2.218SerArg: 2.218 ± 0.113
5.591SerSer: 5.591 ± 0.263
3.518SerThr: 3.518 ± 0.207
4.426SerVal: 4.426 ± 0.149
0.676SerTrp: 0.676 ± 0.06
3.049SerTyr: 3.049 ± 0.155
0.0SerXaa: 0.0 ± 0.0
Thr
2.807ThrAla: 2.807 ± 0.274
0.382ThrCys: 0.382 ± 0.045
2.914ThrAsp: 2.914 ± 0.15
3.286ThrGlu: 3.286 ± 0.135
2.426ThrPhe: 2.426 ± 0.117
4.325ThrGly: 4.325 ± 0.33
0.812ThrHis: 0.812 ± 0.06
4.953ThrIle: 4.953 ± 0.161
4.426ThrLys: 4.426 ± 0.152
4.658ThrLeu: 4.658 ± 0.141
0.817ThrMet: 0.817 ± 0.071
3.122ThrAsn: 3.122 ± 0.167
2.387ThrPro: 2.387 ± 0.174
1.073ThrGln: 1.073 ± 0.087
1.691ThrArg: 1.691 ± 0.09
3.779ThrSer: 3.779 ± 0.263
3.595ThrThr: 3.595 ± 0.451
2.894ThrVal: 2.894 ± 0.126
0.512ThrTrp: 0.512 ± 0.051
1.86ThrTyr: 1.86 ± 0.117
0.0ThrXaa: 0.0 ± 0.0
Val
3.711ValAla: 3.711 ± 0.143
0.551ValCys: 0.551 ± 0.059
3.204ValAsp: 3.204 ± 0.133
3.735ValGlu: 3.735 ± 0.153
3.194ValPhe: 3.194 ± 0.125
4.745ValGly: 4.745 ± 0.206
0.826ValHis: 0.826 ± 0.063
5.658ValIle: 5.658 ± 0.179
4.755ValLys: 4.755 ± 0.158
5.528ValLeu: 5.528 ± 0.156
1.252ValMet: 1.252 ± 0.072
2.827ValAsn: 2.827 ± 0.129
2.073ValPro: 2.073 ± 0.093
0.981ValGln: 0.981 ± 0.073
2.087ValArg: 2.087 ± 0.111
5.286ValSer: 5.286 ± 0.161
2.846ValThr: 2.846 ± 0.165
4.117ValVal: 4.117 ± 0.147
0.59ValTrp: 0.59 ± 0.057
2.179ValTyr: 2.179 ± 0.14
0.0ValXaa: 0.0 ± 0.0
Trp
0.483TrpAla: 0.483 ± 0.049
0.072TrpCys: 0.072 ± 0.018
0.546TrpAsp: 0.546 ± 0.05
0.633TrpGlu: 0.633 ± 0.06
0.449TrpPhe: 0.449 ± 0.044
0.667TrpGly: 0.667 ± 0.057
0.193TrpHis: 0.193 ± 0.034
0.667TrpIle: 0.667 ± 0.066
0.797TrpLys: 0.797 ± 0.062
0.696TrpLeu: 0.696 ± 0.06
0.3TrpMet: 0.3 ± 0.034
0.507TrpAsn: 0.507 ± 0.045
0.179TrpPro: 0.179 ± 0.03
0.3TrpGln: 0.3 ± 0.034
0.377TrpArg: 0.377 ± 0.051
0.561TrpSer: 0.561 ± 0.061
0.541TrpThr: 0.541 ± 0.067
0.58TrpVal: 0.58 ± 0.051
0.116TrpTrp: 0.116 ± 0.025
0.329TrpTyr: 0.329 ± 0.04
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.851TyrAla: 1.851 ± 0.097
0.488TyrCys: 0.488 ± 0.056
2.025TyrAsp: 2.025 ± 0.096
2.329TyrGlu: 2.329 ± 0.115
2.252TyrPhe: 2.252 ± 0.107
2.691TyrGly: 2.691 ± 0.128
0.57TyrHis: 0.57 ± 0.051
3.146TyrIle: 3.146 ± 0.119
3.571TyrLys: 3.571 ± 0.137
3.871TyrLeu: 3.871 ± 0.174
0.623TyrMet: 0.623 ± 0.053
2.329TyrAsn: 2.329 ± 0.11
1.358TyrPro: 1.358 ± 0.091
1.015TyrGln: 1.015 ± 0.072
1.189TyrArg: 1.189 ± 0.089
2.406TyrSer: 2.406 ± 0.132
1.933TyrThr: 1.933 ± 0.11
2.112TyrVal: 2.112 ± 0.11
0.353TyrTrp: 0.353 ± 0.041
1.551TyrTyr: 1.551 ± 0.103
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.005XaaGlu: 0.005 ± 0.005
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.005XaaLys: 0.005 ± 0.004
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.159XaaXaa: 0.159 ± 0.11
Statistics based on 686 proteins (206949 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski