Chitinophaga skermanii

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Chitinophaga

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5113 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A327QEH1|A0A327QEH1_9BACT Putative outer membrane starch-binding protein OS=Chitinophaga skermanii OX=331697 GN=LX64_03418 PE=3 SV=1
MM1 pKa = 7.72KK2 pKa = 10.65NFTFYY7 pKa = 11.02KK8 pKa = 10.15EE9 pKa = 3.84DD10 pKa = 3.52SRR12 pKa = 11.84WYY14 pKa = 9.25IDD16 pKa = 3.64LPEE19 pKa = 5.44FIAQGGDD26 pKa = 3.15KK27 pKa = 11.22AEE29 pKa = 4.26LEE31 pKa = 4.47MVLGADD37 pKa = 3.84TLLEE41 pKa = 4.53LYY43 pKa = 10.87AEE45 pKa = 5.14GNTSVHH51 pKa = 5.69IQMDD55 pKa = 3.98IYY57 pKa = 10.53PFDD60 pKa = 3.68FAEE63 pKa = 4.09VLILQSQKK71 pKa = 11.01AYY73 pKa = 10.12EE74 pKa = 4.18LGGGADD80 pKa = 3.73YY81 pKa = 10.91ILPFFGGIAVDD92 pKa = 3.89MPVWLCDD99 pKa = 3.09VTLFVFDD106 pKa = 4.01TFPAEE111 pKa = 3.65IYY113 pKa = 9.95LRR115 pKa = 11.84KK116 pKa = 9.29IDD118 pKa = 3.49

Molecular weight: 13.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A327QUR5|A0A327QUR5_9BACT Two-component sensor histidine kinase OS=Chitinophaga skermanii OX=331697 GN=LX64_01007 PE=4 SV=1
MM1 pKa = 7.69PKK3 pKa = 10.34VKK5 pKa = 8.9THH7 pKa = 5.39SRR9 pKa = 11.84AKK11 pKa = 9.54KK12 pKa = 7.24TFKK15 pKa = 10.07VTGSGNIKK23 pKa = 9.87RR24 pKa = 11.84PKK26 pKa = 9.9AFKK29 pKa = 10.61SHH31 pKa = 7.2LLTKK35 pKa = 10.39KK36 pKa = 7.65ATKK39 pKa = 10.02RR40 pKa = 11.84KK41 pKa = 7.84RR42 pKa = 11.84HH43 pKa = 5.56LRR45 pKa = 11.84GSSLVSDD52 pKa = 4.28ANLNLVKK59 pKa = 10.67RR60 pKa = 11.84MLRR63 pKa = 11.84LRR65 pKa = 3.95

Molecular weight: 7.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5113

0

5113

1778925

10

7238

347.9

38.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.003 ± 0.034

0.842 ± 0.019

5.037 ± 0.027

5.374 ± 0.05

4.773 ± 0.029

6.704 ± 0.039

2.046 ± 0.027

6.883 ± 0.027

6.457 ± 0.05

9.148 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.504 ± 0.024

5.602 ± 0.049

4.076 ± 0.023

4.245 ± 0.029

3.878 ± 0.024

5.891 ± 0.032

6.331 ± 0.092

6.767 ± 0.043

1.209 ± 0.016

4.228 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski