Giardia intestinalis (strain ATCC 50803 / WB clone C6) (Giardia lamblia)

Taxonomy: cellular organisms; Eukaryota; Metamonada; Fornicata; Diplomonadida; Hexamitidae; Giardiinae; Giardia; Giardia intestinalis

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4900 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A8B976|A8B976_GIAIC MT associated TRAF3 interacting protein OS=Giardia intestinalis (strain ATCC 50803 / WB clone C6) OX=184922 GN=GL50803_009098 PE=4 SV=1
MM1 pKa = 7.4TEE3 pKa = 4.01EE4 pKa = 4.08EE5 pKa = 4.01QLIDD9 pKa = 3.45IEE11 pKa = 5.06SVFSVVGDD19 pKa = 3.73FGGSVTMEE27 pKa = 4.31LADD30 pKa = 3.82GAIVYY35 pKa = 9.45HH36 pKa = 6.75AFYY39 pKa = 11.14DD40 pKa = 4.23LAQAGIFKK48 pKa = 10.74

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8BAL1|A8BAL1_GIAIC Uncharacterized protein OS=Giardia intestinalis (strain ATCC 50803 / WB clone C6) OX=184922 GN=GL50803_0013539 PE=4 SV=1
MM1 pKa = 7.35SSDD4 pKa = 3.17VKK6 pKa = 10.56RR7 pKa = 11.84FRR9 pKa = 11.84WEE11 pKa = 3.68KK12 pKa = 10.7KK13 pKa = 9.85KK14 pKa = 11.04FSTFPLGSLCRR25 pKa = 11.84SSRR28 pKa = 11.84CLPRR32 pKa = 11.84LSHH35 pKa = 6.92LLAFRR40 pKa = 11.84VSCRR44 pKa = 11.84LISSEE49 pKa = 4.47HH50 pKa = 5.75PLSRR54 pKa = 11.84AFPSATAVWPQKK66 pKa = 10.69QLTLAMYY73 pKa = 10.55SRR75 pKa = 11.84VALVLVLYY83 pKa = 10.74HH84 pKa = 6.74EE85 pKa = 4.69DD86 pKa = 3.93RR87 pKa = 11.84IRR89 pKa = 11.84HH90 pKa = 5.29EE91 pKa = 4.26PQVSIPRR98 pKa = 11.84SLRR101 pKa = 11.84CSLVFSWRR109 pKa = 11.84VPSVPSMIGRR119 pKa = 11.84VLPVCLYY126 pKa = 10.29LRR128 pKa = 11.84VVVRR132 pKa = 11.84AHH134 pKa = 6.17GRR136 pKa = 11.84RR137 pKa = 11.84LDD139 pKa = 3.33HH140 pKa = 7.19SYY142 pKa = 11.58VDD144 pKa = 3.8GLAGVWKK151 pKa = 10.46EE152 pKa = 3.92EE153 pKa = 3.63VRR155 pKa = 11.84LWFSLSWSTHH165 pKa = 4.93TFCSWITVHH174 pKa = 7.15LFFDD178 pKa = 4.09IMM180 pKa = 4.05

Molecular weight: 21.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4900

0

4900

3121753

42

8161

637.1

70.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.995 ± 0.046

2.487 ± 0.045

5.462 ± 0.021

5.897 ± 0.042

3.488 ± 0.024

5.271 ± 0.04

2.387 ± 0.014

5.735 ± 0.027

5.096 ± 0.027

10.511 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.207 ± 0.013

4.084 ± 0.018

4.626 ± 0.026

4.02 ± 0.022

5.274 ± 0.025

9.155 ± 0.045

6.406 ± 0.021

5.906 ± 0.018

0.635 ± 0.008

3.358 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski