Hortaea thailandica

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; dothideomyceta; Dothideomycetes; Dothideomycetidae; Mycosphaerellales; Teratosphaeriaceae; Hortaea

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8771 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4U0UA26|A0A4U0UA26_9PEZI Uncharacterized protein OS=Hortaea thailandica OX=706561 GN=B0A50_01365 PE=4 SV=1
MM1 pKa = 7.13FASFLLLSGLAALAAAQSTVLTFTHH26 pKa = 6.16VPNPVTDD33 pKa = 4.81GEE35 pKa = 4.3AQALTFSTDD44 pKa = 3.33DD45 pKa = 3.19ASNPVTITLLQGAANNLQTVSVLTTNAVNGQYY77 pKa = 10.6VWTPSADD84 pKa = 3.7LPNGADD90 pKa = 3.4YY91 pKa = 11.29ALEE94 pKa = 4.03IQQAGEE100 pKa = 4.05TNYY103 pKa = 10.24FGPFTVQGAGSVSSAMSSASSMPASSSMISASSMAPTSSMAPTSYY148 pKa = 9.89MAPTSSMLPASSMAPVSTAPSMSMNSTAATNTPMPVGTMGTAIMMNGTIYY198 pKa = 10.5PSGTAMPRR206 pKa = 11.84NTTMSMATLTPTTAMGGSPSTNSAAATGGSSAAPASSSMATNGATGTIAALSTTFLDD263 pKa = 5.4LYY265 pKa = 10.97NKK267 pKa = 8.43TSNMSPRR274 pKa = 11.84SLLLALVSAPLLAFAQSANPFSIPDD299 pKa = 3.66AGLSATGGQPLEE311 pKa = 4.77LMWNPTTDD319 pKa = 3.24GTVTLVLRR327 pKa = 11.84SGNADD332 pKa = 3.16NLAEE336 pKa = 4.2GTVIASNIDD345 pKa = 3.26NSGSYY350 pKa = 8.25TWTPSDD356 pKa = 3.41SLTRR360 pKa = 11.84GSDD363 pKa = 3.41YY364 pKa = 10.83TIEE367 pKa = 4.37IVDD370 pKa = 5.01DD371 pKa = 4.65DD372 pKa = 4.87DD373 pKa = 4.67VSNTNFTPYY382 pKa = 10.12FVLDD386 pKa = 3.46TTTTVAQSTSQVTLGAPTEE405 pKa = 4.37TPDD408 pKa = 4.28KK409 pKa = 11.0SSASPTGAATSVLDD423 pKa = 3.56ATMDD427 pKa = 3.64TSSAAATTMSSMSADD442 pKa = 3.46SSSAPASSSMASSSPTSMDD461 pKa = 3.14AAATTSAPAGKK472 pKa = 8.13MTMSAGNMTMGAGAPSAGSSTNSSASPSSSVEE504 pKa = 3.74PFIGGAASLKK514 pKa = 10.42VSAALGAFGLVALCAMVLL532 pKa = 3.58

Molecular weight: 52.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4U0TX96|A0A4U0TX96_9PEZI Carboxylic ester hydrolase OS=Hortaea thailandica OX=706561 GN=B0A50_05291 PE=3 SV=1
MM1 pKa = 7.35AAVVTPSSQPAPVARR16 pKa = 11.84APAARR21 pKa = 11.84VPAARR26 pKa = 11.84APVAKK31 pKa = 10.57APVAPVARR39 pKa = 11.84PVAATRR45 pKa = 11.84TAAAPVASSVKK56 pKa = 9.39PVTPARR62 pKa = 11.84PAVKK66 pKa = 10.14SPSTVPVAGRR76 pKa = 11.84PTGTSAPVTTGAATRR91 pKa = 11.84APARR95 pKa = 11.84SAAPVGVSGAAAVTPRR111 pKa = 11.84TTARR115 pKa = 11.84TTAATPAVANPVVPGRR131 pKa = 11.84RR132 pKa = 11.84AVGTTAATPVASRR145 pKa = 11.84TAAVTGTTAATRR157 pKa = 11.84TAVPATGAVPRR168 pKa = 11.84TAAPTTVGAAGVRR181 pKa = 11.84SAPVTTNGVRR191 pKa = 11.84TARR194 pKa = 11.84AAPATTAAVTPAGTTVARR212 pKa = 11.84PRR214 pKa = 11.84TATTGAATRR223 pKa = 11.84SGVVGAGRR231 pKa = 11.84KK232 pKa = 8.07VVGGGVAQPVSGNVAPVNNTIAGVRR257 pKa = 11.84QPVAGPGAVNGVVGNSVNTVGGPGRR282 pKa = 11.84AAIGSTAPAARR293 pKa = 11.84TVTPATSSVASTPVGSAAVTPAATRR318 pKa = 11.84AATPAVSGVAGGVAGAARR336 pKa = 11.84TVGGAVSSVGGAVPGVAGAARR357 pKa = 11.84PVGGAVSSVGGAVPGVAGAARR378 pKa = 11.84PVGGAVGGVGGAVADD393 pKa = 4.3PVTGAASTVGGAVPAAPTSAAAVPGVAGASGVVGNAVSGVGSTVAGVGRR442 pKa = 11.84SLGGLASGVVGG453 pKa = 4.44

Molecular weight: 40.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8771

0

8771

4524954

51

7619

515.9

56.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.491 ± 0.022

1.095 ± 0.008

5.753 ± 0.019

6.429 ± 0.025

3.399 ± 0.017

7.442 ± 0.025

2.403 ± 0.01

4.11 ± 0.018

4.687 ± 0.024

8.558 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.226 ± 0.01

3.402 ± 0.015

6.191 ± 0.031

4.402 ± 0.022

6.315 ± 0.023

7.984 ± 0.034

6.059 ± 0.02

6.018 ± 0.018

1.372 ± 0.01

2.664 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski