Amino acid dipepetide frequency for Mycobacterium phage HINdeR

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.816AlaAla: 10.816 ± 0.907
0.661AlaCys: 0.661 ± 0.172
6.91AlaAsp: 6.91 ± 0.752
5.588AlaGlu: 5.588 ± 0.647
4.086AlaPhe: 4.086 ± 0.501
7.992AlaGly: 7.992 ± 1.003
2.343AlaHis: 2.343 ± 0.351
4.447AlaIle: 4.447 ± 0.547
3.786AlaLys: 3.786 ± 0.58
7.691AlaLeu: 7.691 ± 0.708
3.065AlaMet: 3.065 ± 0.459
2.464AlaAsn: 2.464 ± 0.393
4.507AlaPro: 4.507 ± 0.597
4.146AlaGln: 4.146 ± 0.577
4.627AlaArg: 4.627 ± 0.673
4.627AlaSer: 4.627 ± 0.589
5.228AlaThr: 5.228 ± 0.509
7.571AlaVal: 7.571 ± 0.869
2.163AlaTrp: 2.163 ± 0.333
2.704AlaTyr: 2.704 ± 0.537
0.0AlaXaa: 0.0 ± 0.0
Cys
0.601CysAla: 0.601 ± 0.188
0.12CysCys: 0.12 ± 0.114
0.421CysAsp: 0.421 ± 0.169
0.721CysGlu: 0.721 ± 0.216
0.24CysPhe: 0.24 ± 0.125
0.781CysGly: 0.781 ± 0.224
0.24CysHis: 0.24 ± 0.128
0.481CysIle: 0.481 ± 0.197
0.3CysLys: 0.3 ± 0.143
0.781CysLeu: 0.781 ± 0.233
0.12CysMet: 0.12 ± 0.087
0.421CysAsn: 0.421 ± 0.166
0.3CysPro: 0.3 ± 0.151
0.18CysGln: 0.18 ± 0.117
0.661CysArg: 0.661 ± 0.203
0.24CysSer: 0.24 ± 0.123
0.3CysThr: 0.3 ± 0.155
0.841CysVal: 0.841 ± 0.253
0.3CysTrp: 0.3 ± 0.129
0.18CysTyr: 0.18 ± 0.101
0.0CysXaa: 0.0 ± 0.0
Asp
6.369AspAla: 6.369 ± 0.653
0.541AspCys: 0.541 ± 0.199
3.786AspAsp: 3.786 ± 0.491
4.507AspGlu: 4.507 ± 0.631
2.223AspPhe: 2.223 ± 0.466
6.189AspGly: 6.189 ± 0.663
1.382AspHis: 1.382 ± 0.348
2.163AspIle: 2.163 ± 0.389
2.644AspLys: 2.644 ± 0.42
5.708AspLeu: 5.708 ± 0.641
1.382AspMet: 1.382 ± 0.341
1.743AspAsn: 1.743 ± 0.308
4.867AspPro: 4.867 ± 0.572
1.803AspGln: 1.803 ± 0.264
3.425AspArg: 3.425 ± 0.543
2.944AspSer: 2.944 ± 0.431
3.726AspThr: 3.726 ± 0.498
4.867AspVal: 4.867 ± 0.484
1.562AspTrp: 1.562 ± 0.283
1.622AspTyr: 1.622 ± 0.292
0.0AspXaa: 0.0 ± 0.0
Glu
5.708GluAla: 5.708 ± 0.58
0.361GluCys: 0.361 ± 0.16
4.867GluAsp: 4.867 ± 0.618
3.545GluGlu: 3.545 ± 0.503
2.043GluPhe: 2.043 ± 0.397
5.408GluGly: 5.408 ± 0.728
1.502GluHis: 1.502 ± 0.423
2.884GluIle: 2.884 ± 0.435
2.404GluLys: 2.404 ± 0.332
5.468GluLeu: 5.468 ± 0.59
1.562GluMet: 1.562 ± 0.339
1.322GluAsn: 1.322 ± 0.27
2.884GluPro: 2.884 ± 0.401
2.464GluGln: 2.464 ± 0.399
4.206GluArg: 4.206 ± 0.568
3.245GluSer: 3.245 ± 0.544
2.644GluThr: 2.644 ± 0.392
5.288GluVal: 5.288 ± 0.649
1.442GluTrp: 1.442 ± 0.291
1.682GluTyr: 1.682 ± 0.374
0.0GluXaa: 0.0 ± 0.0
Phe
2.884PheAla: 2.884 ± 0.486
0.3PheCys: 0.3 ± 0.134
2.404PheAsp: 2.404 ± 0.373
2.464PheGlu: 2.464 ± 0.397
0.661PhePhe: 0.661 ± 0.208
3.786PheGly: 3.786 ± 0.445
0.361PheHis: 0.361 ± 0.141
1.863PheIle: 1.863 ± 0.317
1.202PheLys: 1.202 ± 0.235
2.824PheLeu: 2.824 ± 0.376
0.541PheMet: 0.541 ± 0.215
1.202PheAsn: 1.202 ± 0.287
2.103PhePro: 2.103 ± 0.368
1.502PheGln: 1.502 ± 0.297
1.682PheArg: 1.682 ± 0.338
1.983PheSer: 1.983 ± 0.334
2.223PheThr: 2.223 ± 0.372
2.464PheVal: 2.464 ± 0.496
0.601PheTrp: 0.601 ± 0.195
0.541PheTyr: 0.541 ± 0.162
0.0PheXaa: 0.0 ± 0.0
Gly
7.511GlyAla: 7.511 ± 0.996
0.961GlyCys: 0.961 ± 0.243
5.949GlyAsp: 5.949 ± 0.905
4.867GlyGlu: 4.867 ± 0.383
2.704GlyPhe: 2.704 ± 0.393
11.597GlyGly: 11.597 ± 3.073
2.283GlyHis: 2.283 ± 0.353
5.528GlyIle: 5.528 ± 0.596
4.987GlyLys: 4.987 ± 0.493
7.511GlyLeu: 7.511 ± 0.742
2.103GlyMet: 2.103 ± 0.322
3.365GlyAsn: 3.365 ± 0.734
7.211GlyPro: 7.211 ± 2.138
3.185GlyGln: 3.185 ± 0.426
4.807GlyArg: 4.807 ± 0.717
4.747GlySer: 4.747 ± 0.714
5.949GlyThr: 5.949 ± 0.802
6.249GlyVal: 6.249 ± 0.651
2.464GlyTrp: 2.464 ± 0.397
2.704GlyTyr: 2.704 ± 0.325
0.0GlyXaa: 0.0 ± 0.0
His
1.682HisAla: 1.682 ± 0.396
0.12HisCys: 0.12 ± 0.085
1.502HisAsp: 1.502 ± 0.278
1.382HisGlu: 1.382 ± 0.32
0.961HisPhe: 0.961 ± 0.203
2.043HisGly: 2.043 ± 0.392
0.481HisHis: 0.481 ± 0.171
0.901HisIle: 0.901 ± 0.274
0.781HisLys: 0.781 ± 0.2
1.562HisLeu: 1.562 ± 0.383
0.18HisMet: 0.18 ± 0.11
0.361HisAsn: 0.361 ± 0.174
1.202HisPro: 1.202 ± 0.252
0.661HisGln: 0.661 ± 0.241
2.043HisArg: 2.043 ± 0.462
0.961HisSer: 0.961 ± 0.279
1.442HisThr: 1.442 ± 0.307
1.322HisVal: 1.322 ± 0.284
0.541HisTrp: 0.541 ± 0.211
0.481HisTyr: 0.481 ± 0.154
0.0HisXaa: 0.0 ± 0.0
Ile
5.168IleAla: 5.168 ± 0.518
0.361IleCys: 0.361 ± 0.128
2.944IleAsp: 2.944 ± 0.397
3.485IleGlu: 3.485 ± 0.474
1.142IlePhe: 1.142 ± 0.277
3.966IleGly: 3.966 ± 0.448
1.262IleHis: 1.262 ± 0.263
1.622IleIle: 1.622 ± 0.284
1.743IleLys: 1.743 ± 0.307
3.004IleLeu: 3.004 ± 0.463
0.661IleMet: 0.661 ± 0.196
2.283IleAsn: 2.283 ± 0.396
4.086IlePro: 4.086 ± 0.513
1.983IleGln: 1.983 ± 0.485
3.245IleArg: 3.245 ± 0.44
2.524IleSer: 2.524 ± 0.382
3.365IleThr: 3.365 ± 0.412
3.245IleVal: 3.245 ± 0.54
0.781IleTrp: 0.781 ± 0.241
0.901IleTyr: 0.901 ± 0.228
0.0IleXaa: 0.0 ± 0.0
Lys
4.627LysAla: 4.627 ± 0.471
0.12LysCys: 0.12 ± 0.079
2.764LysAsp: 2.764 ± 0.381
1.923LysGlu: 1.923 ± 0.417
1.082LysPhe: 1.082 ± 0.23
4.867LysGly: 4.867 ± 0.581
0.601LysHis: 0.601 ± 0.191
2.404LysIle: 2.404 ± 0.477
2.404LysLys: 2.404 ± 0.502
4.086LysLeu: 4.086 ± 0.581
0.541LysMet: 0.541 ± 0.178
1.262LysAsn: 1.262 ± 0.273
2.944LysPro: 2.944 ± 0.396
1.202LysGln: 1.202 ± 0.269
2.584LysArg: 2.584 ± 0.467
1.442LysSer: 1.442 ± 0.259
2.644LysThr: 2.644 ± 0.381
3.786LysVal: 3.786 ± 0.546
0.661LysTrp: 0.661 ± 0.18
1.262LysTyr: 1.262 ± 0.284
0.0LysXaa: 0.0 ± 0.0
Leu
8.172LeuAla: 8.172 ± 0.903
0.541LeuCys: 0.541 ± 0.215
5.108LeuAsp: 5.108 ± 0.511
4.507LeuGlu: 4.507 ± 0.611
2.103LeuPhe: 2.103 ± 0.288
7.992LeuGly: 7.992 ± 0.848
1.442LeuHis: 1.442 ± 0.272
3.485LeuIle: 3.485 ± 0.373
3.365LeuLys: 3.365 ± 0.47
5.708LeuLeu: 5.708 ± 0.621
2.464LeuMet: 2.464 ± 0.352
2.824LeuAsn: 2.824 ± 0.483
4.567LeuPro: 4.567 ± 0.536
3.125LeuGln: 3.125 ± 0.512
5.769LeuArg: 5.769 ± 0.547
4.867LeuSer: 4.867 ± 0.565
5.168LeuThr: 5.168 ± 0.719
5.108LeuVal: 5.108 ± 0.579
1.682LeuTrp: 1.682 ± 0.344
2.644LeuTyr: 2.644 ± 0.48
0.0LeuXaa: 0.0 ± 0.0
Met
1.863MetAla: 1.863 ± 0.341
0.12MetCys: 0.12 ± 0.09
1.262MetAsp: 1.262 ± 0.287
1.262MetGlu: 1.262 ± 0.284
0.721MetPhe: 0.721 ± 0.19
2.343MetGly: 2.343 ± 0.36
0.541MetHis: 0.541 ± 0.172
1.382MetIle: 1.382 ± 0.272
1.322MetLys: 1.322 ± 0.282
1.682MetLeu: 1.682 ± 0.306
0.661MetMet: 0.661 ± 0.195
0.781MetAsn: 0.781 ± 0.162
1.262MetPro: 1.262 ± 0.279
0.961MetGln: 0.961 ± 0.267
1.442MetArg: 1.442 ± 0.297
2.223MetSer: 2.223 ± 0.349
2.343MetThr: 2.343 ± 0.343
1.322MetVal: 1.322 ± 0.274
0.18MetTrp: 0.18 ± 0.107
0.601MetTyr: 0.601 ± 0.152
0.0MetXaa: 0.0 ± 0.0
Asn
3.065AsnAla: 3.065 ± 0.456
0.24AsnCys: 0.24 ± 0.137
1.803AsnAsp: 1.803 ± 0.303
1.863AsnGlu: 1.863 ± 0.324
1.262AsnPhe: 1.262 ± 0.312
2.944AsnGly: 2.944 ± 0.717
0.601AsnHis: 0.601 ± 0.209
1.502AsnIle: 1.502 ± 0.36
1.562AsnLys: 1.562 ± 0.329
2.704AsnLeu: 2.704 ± 0.398
0.601AsnMet: 0.601 ± 0.17
0.661AsnAsn: 0.661 ± 0.199
2.043AsnPro: 2.043 ± 0.334
1.142AsnGln: 1.142 ± 0.259
2.644AsnArg: 2.644 ± 0.39
2.043AsnSer: 2.043 ± 0.354
2.043AsnThr: 2.043 ± 0.381
2.223AsnVal: 2.223 ± 0.389
0.781AsnTrp: 0.781 ± 0.205
0.721AsnTyr: 0.721 ± 0.177
0.0AsnXaa: 0.0 ± 0.0
Pro
4.927ProAla: 4.927 ± 0.596
0.481ProCys: 0.481 ± 0.207
3.786ProAsp: 3.786 ± 0.496
4.447ProGlu: 4.447 ± 0.603
1.863ProPhe: 1.863 ± 0.366
6.009ProGly: 6.009 ± 0.721
1.202ProHis: 1.202 ± 0.308
2.584ProIle: 2.584 ± 0.319
2.464ProLys: 2.464 ± 0.442
4.386ProLeu: 4.386 ± 0.483
1.022ProMet: 1.022 ± 0.237
2.043ProAsn: 2.043 ± 0.352
2.524ProPro: 2.524 ± 0.515
3.004ProGln: 3.004 ± 0.8
2.524ProArg: 2.524 ± 0.371
3.726ProSer: 3.726 ± 0.453
3.786ProThr: 3.786 ± 0.511
4.026ProVal: 4.026 ± 0.602
1.262ProTrp: 1.262 ± 0.516
1.622ProTyr: 1.622 ± 0.31
0.0ProXaa: 0.0 ± 0.0
Gln
4.807GlnAla: 4.807 ± 0.556
0.18GlnCys: 0.18 ± 0.111
1.622GlnAsp: 1.622 ± 0.295
2.524GlnGlu: 2.524 ± 0.429
1.442GlnPhe: 1.442 ± 0.33
4.326GlnGly: 4.326 ± 1.409
0.721GlnHis: 0.721 ± 0.192
2.163GlnIle: 2.163 ± 0.403
1.262GlnLys: 1.262 ± 0.266
2.884GlnLeu: 2.884 ± 0.642
1.262GlnMet: 1.262 ± 0.306
1.142GlnAsn: 1.142 ± 0.295
1.622GlnPro: 1.622 ± 0.358
1.743GlnGln: 1.743 ± 0.445
2.944GlnArg: 2.944 ± 0.374
1.502GlnSer: 1.502 ± 0.337
2.524GlnThr: 2.524 ± 0.449
2.764GlnVal: 2.764 ± 0.516
0.901GlnTrp: 0.901 ± 0.263
0.901GlnTyr: 0.901 ± 0.21
0.0GlnXaa: 0.0 ± 0.0
Arg
4.627ArgAla: 4.627 ± 0.628
1.022ArgCys: 1.022 ± 0.324
3.065ArgAsp: 3.065 ± 0.4
4.326ArgGlu: 4.326 ± 0.533
2.223ArgPhe: 2.223 ± 0.403
4.086ArgGly: 4.086 ± 0.498
1.442ArgHis: 1.442 ± 0.341
3.004ArgIle: 3.004 ± 0.366
3.485ArgLys: 3.485 ± 0.559
5.348ArgLeu: 5.348 ± 0.663
2.283ArgMet: 2.283 ± 0.358
2.103ArgAsn: 2.103 ± 0.349
2.644ArgPro: 2.644 ± 0.438
2.524ArgGln: 2.524 ± 0.431
5.108ArgArg: 5.108 ± 0.676
2.644ArgSer: 2.644 ± 0.489
3.065ArgThr: 3.065 ± 0.416
3.906ArgVal: 3.906 ± 0.446
1.382ArgTrp: 1.382 ± 0.3
2.584ArgTyr: 2.584 ± 0.458
0.0ArgXaa: 0.0 ± 0.0
Ser
4.567SerAla: 4.567 ± 0.6
0.361SerCys: 0.361 ± 0.129
3.846SerAsp: 3.846 ± 0.58
3.305SerGlu: 3.305 ± 0.414
1.983SerPhe: 1.983 ± 0.36
6.43SerGly: 6.43 ± 0.853
0.781SerHis: 0.781 ± 0.231
2.163SerIle: 2.163 ± 0.427
2.704SerLys: 2.704 ± 0.508
5.047SerLeu: 5.047 ± 0.506
1.502SerMet: 1.502 ± 0.326
1.863SerAsn: 1.863 ± 0.315
2.464SerPro: 2.464 ± 0.407
2.343SerGln: 2.343 ± 0.435
3.545SerArg: 3.545 ± 0.613
2.223SerSer: 2.223 ± 0.472
2.704SerThr: 2.704 ± 0.518
3.004SerVal: 3.004 ± 0.42
0.841SerTrp: 0.841 ± 0.225
1.262SerTyr: 1.262 ± 0.323
0.0SerXaa: 0.0 ± 0.0
Thr
6.67ThrAla: 6.67 ± 0.593
0.601ThrCys: 0.601 ± 0.189
3.305ThrAsp: 3.305 ± 0.52
2.944ThrGlu: 2.944 ± 0.336
2.404ThrPhe: 2.404 ± 0.535
6.91ThrGly: 6.91 ± 0.776
1.142ThrHis: 1.142 ± 0.239
2.704ThrIle: 2.704 ± 0.417
2.163ThrLys: 2.163 ± 0.358
5.228ThrLeu: 5.228 ± 0.679
1.322ThrMet: 1.322 ± 0.299
2.043ThrAsn: 2.043 ± 0.301
4.266ThrPro: 4.266 ± 0.595
2.283ThrGln: 2.283 ± 0.471
2.644ThrArg: 2.644 ± 0.453
3.485ThrSer: 3.485 ± 0.552
4.507ThrThr: 4.507 ± 0.763
4.687ThrVal: 4.687 ± 0.578
1.022ThrTrp: 1.022 ± 0.187
1.082ThrTyr: 1.082 ± 0.253
0.0ThrXaa: 0.0 ± 0.0
Val
7.511ValAla: 7.511 ± 0.607
0.601ValCys: 0.601 ± 0.183
4.326ValAsp: 4.326 ± 0.532
4.627ValGlu: 4.627 ± 0.564
2.764ValPhe: 2.764 ± 0.363
5.408ValGly: 5.408 ± 0.677
1.322ValHis: 1.322 ± 0.261
3.545ValIle: 3.545 ± 0.505
3.305ValLys: 3.305 ± 0.571
4.927ValLeu: 4.927 ± 0.606
1.622ValMet: 1.622 ± 0.302
2.283ValAsn: 2.283 ± 0.375
3.906ValPro: 3.906 ± 0.563
2.884ValGln: 2.884 ± 0.398
4.026ValArg: 4.026 ± 0.495
4.687ValSer: 4.687 ± 0.633
4.927ValThr: 4.927 ± 0.538
6.129ValVal: 6.129 ± 0.598
1.082ValTrp: 1.082 ± 0.279
2.584ValTyr: 2.584 ± 0.512
0.0ValXaa: 0.0 ± 0.0
Trp
1.682TrpAla: 1.682 ± 0.408
0.3TrpCys: 0.3 ± 0.12
1.622TrpAsp: 1.622 ± 0.293
0.961TrpGlu: 0.961 ± 0.206
0.661TrpPhe: 0.661 ± 0.21
1.562TrpGly: 1.562 ± 0.306
0.421TrpHis: 0.421 ± 0.159
1.442TrpIle: 1.442 ± 0.289
0.481TrpLys: 0.481 ± 0.172
1.622TrpLeu: 1.622 ± 0.294
0.601TrpMet: 0.601 ± 0.196
0.961TrpAsn: 0.961 ± 0.325
1.082TrpPro: 1.082 ± 0.246
0.901TrpGln: 0.901 ± 0.229
1.082TrpArg: 1.082 ± 0.231
1.382TrpSer: 1.382 ± 0.282
1.202TrpThr: 1.202 ± 0.301
1.622TrpVal: 1.622 ± 0.307
0.601TrpTrp: 0.601 ± 0.233
0.481TrpTyr: 0.481 ± 0.163
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.223TyrAla: 2.223 ± 0.32
0.24TyrCys: 0.24 ± 0.133
2.163TyrAsp: 2.163 ± 0.371
1.682TyrGlu: 1.682 ± 0.373
1.022TyrPhe: 1.022 ± 0.255
2.043TyrGly: 2.043 ± 0.317
0.541TyrHis: 0.541 ± 0.173
1.502TyrIle: 1.502 ± 0.331
0.841TyrLys: 0.841 ± 0.215
2.464TyrLeu: 2.464 ± 0.379
0.721TyrMet: 0.721 ± 0.287
1.322TyrAsn: 1.322 ± 0.245
1.202TyrPro: 1.202 ± 0.24
1.202TyrGln: 1.202 ± 0.273
1.863TyrArg: 1.863 ± 0.327
1.442TyrSer: 1.442 ± 0.327
1.622TyrThr: 1.622 ± 0.309
2.043TyrVal: 2.043 ± 0.354
0.421TyrTrp: 0.421 ± 0.155
0.901TyrTyr: 0.901 ± 0.279
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 84 proteins (16643 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski