Amino acid dipepetide frequency for Sporolactobacillus inulinus CASD

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.011AlaAla: 8.011 ± 0.131
0.822AlaCys: 0.822 ± 0.033
4.381AlaAsp: 4.381 ± 0.068
5.021AlaGlu: 5.021 ± 0.1
3.794AlaPhe: 3.794 ± 0.072
5.611AlaGly: 5.611 ± 0.095
1.78AlaHis: 1.78 ± 0.051
5.888AlaIle: 5.888 ± 0.096
5.046AlaLys: 5.046 ± 0.091
8.982AlaLeu: 8.982 ± 0.114
2.265AlaMet: 2.265 ± 0.049
2.689AlaAsn: 2.689 ± 0.054
2.446AlaPro: 2.446 ± 0.062
3.091AlaGln: 3.091 ± 0.066
3.638AlaArg: 3.638 ± 0.074
4.737AlaSer: 4.737 ± 0.087
3.617AlaThr: 3.617 ± 0.071
6.279AlaVal: 6.279 ± 0.1
0.684AlaTrp: 0.684 ± 0.03
2.588AlaTyr: 2.588 ± 0.059
0.0AlaXaa: 0.0 ± 0.0
Cys
0.759CysAla: 0.759 ± 0.033
0.127CysCys: 0.127 ± 0.015
0.466CysAsp: 0.466 ± 0.026
0.44CysGlu: 0.44 ± 0.025
0.442CysPhe: 0.442 ± 0.022
0.858CysGly: 0.858 ± 0.039
0.245CysHis: 0.245 ± 0.017
0.542CysIle: 0.542 ± 0.025
0.375CysLys: 0.375 ± 0.024
0.873CysLeu: 0.873 ± 0.031
0.204CysMet: 0.204 ± 0.016
0.255CysAsn: 0.255 ± 0.02
0.452CysPro: 0.452 ± 0.025
0.24CysGln: 0.24 ± 0.019
0.483CysArg: 0.483 ± 0.026
0.596CysSer: 0.596 ± 0.028
0.483CysThr: 0.483 ± 0.026
0.513CysVal: 0.513 ± 0.022
0.096CysTrp: 0.096 ± 0.011
0.3CysTyr: 0.3 ± 0.022
0.0CysXaa: 0.0 ± 0.0
Asp
4.236AspAla: 4.236 ± 0.071
0.456AspCys: 0.456 ± 0.028
2.716AspAsp: 2.716 ± 0.071
4.015AspGlu: 4.015 ± 0.09
2.303AspPhe: 2.303 ± 0.056
3.446AspGly: 3.446 ± 0.07
1.665AspHis: 1.665 ± 0.047
3.064AspIle: 3.064 ± 0.075
2.756AspLys: 2.756 ± 0.068
5.511AspLeu: 5.511 ± 0.088
1.204AspMet: 1.204 ± 0.042
1.547AspAsn: 1.547 ± 0.047
2.389AspPro: 2.389 ± 0.06
3.065AspGln: 3.065 ± 0.071
3.019AspArg: 3.019 ± 0.058
2.704AspSer: 2.704 ± 0.05
2.441AspThr: 2.441 ± 0.057
3.795AspVal: 3.795 ± 0.072
0.635AspTrp: 0.635 ± 0.028
2.282AspTyr: 2.282 ± 0.064
0.0AspXaa: 0.0 ± 0.0
Glu
5.349GluAla: 5.349 ± 0.096
0.435GluCys: 0.435 ± 0.023
2.973GluAsp: 2.973 ± 0.07
4.296GluGlu: 4.296 ± 0.092
1.749GluPhe: 1.749 ± 0.048
3.556GluGly: 3.556 ± 0.069
1.679GluHis: 1.679 ± 0.05
3.975GluIle: 3.975 ± 0.075
5.467GluLys: 5.467 ± 0.099
6.189GluLeu: 6.189 ± 0.1
1.888GluMet: 1.888 ± 0.053
2.789GluAsn: 2.789 ± 0.06
1.873GluPro: 1.873 ± 0.051
3.295GluGln: 3.295 ± 0.075
3.841GluArg: 3.841 ± 0.078
3.143GluSer: 3.143 ± 0.066
3.468GluThr: 3.468 ± 0.061
3.685GluVal: 3.685 ± 0.079
0.678GluTrp: 0.678 ± 0.025
1.432GluTyr: 1.432 ± 0.044
0.0GluXaa: 0.0 ± 0.0
Phe
3.329PheAla: 3.329 ± 0.073
0.423PheCys: 0.423 ± 0.024
2.567PheAsp: 2.567 ± 0.06
2.273PheGlu: 2.273 ± 0.055
2.333PhePhe: 2.333 ± 0.064
3.352PheGly: 3.352 ± 0.072
1.082PheHis: 1.082 ± 0.038
3.243PheIle: 3.243 ± 0.074
2.257PheLys: 2.257 ± 0.061
4.458PheLeu: 4.458 ± 0.098
1.052PheMet: 1.052 ± 0.032
1.666PheAsn: 1.666 ± 0.047
1.587PhePro: 1.587 ± 0.046
1.598PheGln: 1.598 ± 0.05
1.736PheArg: 1.736 ± 0.052
3.297PheSer: 3.297 ± 0.065
2.382PheThr: 2.382 ± 0.061
3.084PheVal: 3.084 ± 0.072
0.446PheTrp: 0.446 ± 0.027
1.571PheTyr: 1.571 ± 0.051
0.0PheXaa: 0.0 ± 0.0
Gly
5.415GlyAla: 5.415 ± 0.083
0.684GlyCys: 0.684 ± 0.034
3.176GlyAsp: 3.176 ± 0.06
3.719GlyGlu: 3.719 ± 0.072
3.318GlyPhe: 3.318 ± 0.072
4.796GlyGly: 4.796 ± 0.101
1.473GlyHis: 1.473 ± 0.053
5.592GlyIle: 5.592 ± 0.105
5.078GlyLys: 5.078 ± 0.086
6.866GlyLeu: 6.866 ± 0.113
2.218GlyMet: 2.218 ± 0.061
2.377GlyAsn: 2.377 ± 0.055
1.693GlyPro: 1.693 ± 0.045
2.439GlyGln: 2.439 ± 0.059
3.23GlyArg: 3.23 ± 0.066
4.234GlySer: 4.234 ± 0.072
4.11GlyThr: 4.11 ± 0.076
4.909GlyVal: 4.909 ± 0.088
0.763GlyTrp: 0.763 ± 0.036
2.665GlyTyr: 2.665 ± 0.067
0.0GlyXaa: 0.0 ± 0.0
His
1.911HisAla: 1.911 ± 0.054
0.274HisCys: 0.274 ± 0.019
1.173HisAsp: 1.173 ± 0.036
1.537HisGlu: 1.537 ± 0.045
1.271HisPhe: 1.271 ± 0.037
1.589HisGly: 1.589 ± 0.047
0.81HisHis: 0.81 ± 0.032
1.341HisIle: 1.341 ± 0.042
1.037HisLys: 1.037 ± 0.039
2.65HisLeu: 2.65 ± 0.063
0.58HisMet: 0.58 ± 0.03
0.698HisAsn: 0.698 ± 0.027
1.346HisPro: 1.346 ± 0.043
1.157HisGln: 1.157 ± 0.036
1.152HisArg: 1.152 ± 0.036
1.28HisSer: 1.28 ± 0.04
1.073HisThr: 1.073 ± 0.036
1.783HisVal: 1.783 ± 0.048
0.271HisTrp: 0.271 ± 0.019
1.039HisTyr: 1.039 ± 0.033
0.0HisXaa: 0.0 ± 0.0
Ile
6.144IleAla: 6.144 ± 0.096
0.716IleCys: 0.716 ± 0.03
4.599IleAsp: 4.599 ± 0.08
4.528IleGlu: 4.528 ± 0.078
2.733IlePhe: 2.733 ± 0.068
5.99IleGly: 5.99 ± 0.09
1.793IleHis: 1.793 ± 0.051
4.907IleIle: 4.907 ± 0.095
4.173IleLys: 4.173 ± 0.082
6.209IleLeu: 6.209 ± 0.099
1.53IleMet: 1.53 ± 0.047
3.06IleAsn: 3.06 ± 0.062
3.02IlePro: 3.02 ± 0.061
2.632IleGln: 2.632 ± 0.058
3.688IleArg: 3.688 ± 0.074
4.335IleSer: 4.335 ± 0.072
3.558IleThr: 3.558 ± 0.073
5.125IleVal: 5.125 ± 0.082
0.616IleTrp: 0.616 ± 0.035
2.224IleTyr: 2.224 ± 0.054
0.0IleXaa: 0.0 ± 0.0
Lys
5.098LysAla: 5.098 ± 0.088
0.317LysCys: 0.317 ± 0.026
3.325LysAsp: 3.325 ± 0.068
4.723LysGlu: 4.723 ± 0.089
1.595LysPhe: 1.595 ± 0.047
3.945LysGly: 3.945 ± 0.069
1.423LysHis: 1.423 ± 0.041
4.304LysIle: 4.304 ± 0.084
5.94LysLys: 5.94 ± 0.106
5.67LysLeu: 5.67 ± 0.088
2.101LysMet: 2.101 ± 0.054
3.01LysAsn: 3.01 ± 0.065
2.233LysPro: 2.233 ± 0.052
3.315LysGln: 3.315 ± 0.075
3.83LysArg: 3.83 ± 0.077
3.438LysSer: 3.438 ± 0.067
3.779LysThr: 3.779 ± 0.065
4.195LysVal: 4.195 ± 0.076
0.673LysTrp: 0.673 ± 0.027
1.782LysTyr: 1.782 ± 0.055
0.0LysXaa: 0.0 ± 0.0
Leu
8.293LeuAla: 8.293 ± 0.108
0.905LeuCys: 0.905 ± 0.038
5.377LeuAsp: 5.377 ± 0.082
5.431LeuGlu: 5.431 ± 0.102
5.12LeuPhe: 5.12 ± 0.095
6.416LeuGly: 6.416 ± 0.103
2.252LeuHis: 2.252 ± 0.063
7.987LeuIle: 7.987 ± 0.132
6.486LeuLys: 6.486 ± 0.098
10.451LeuLeu: 10.451 ± 0.157
2.655LeuMet: 2.655 ± 0.064
4.544LeuAsn: 4.544 ± 0.077
4.235LeuPro: 4.235 ± 0.06
3.378LeuGln: 3.378 ± 0.066
4.508LeuArg: 4.508 ± 0.085
7.321LeuSer: 7.321 ± 0.108
5.812LeuThr: 5.812 ± 0.086
6.355LeuVal: 6.355 ± 0.105
0.85LeuTrp: 0.85 ± 0.034
2.887LeuTyr: 2.887 ± 0.064
0.0LeuXaa: 0.0 ± 0.0
Met
2.216MetAla: 2.216 ± 0.059
0.151MetCys: 0.151 ± 0.014
1.564MetAsp: 1.564 ± 0.044
1.476MetGlu: 1.476 ± 0.047
0.863MetPhe: 0.863 ± 0.034
1.759MetGly: 1.759 ± 0.051
0.564MetHis: 0.564 ± 0.026
2.433MetIle: 2.433 ± 0.061
2.102MetLys: 2.102 ± 0.046
2.469MetLeu: 2.469 ± 0.066
0.907MetMet: 0.907 ± 0.04
1.541MetAsn: 1.541 ± 0.039
1.161MetPro: 1.161 ± 0.037
0.98MetGln: 0.98 ± 0.039
1.341MetArg: 1.341 ± 0.046
1.579MetSer: 1.579 ± 0.045
1.854MetThr: 1.854 ± 0.049
1.704MetVal: 1.704 ± 0.048
0.146MetTrp: 0.146 ± 0.013
0.631MetTyr: 0.631 ± 0.027
0.0MetXaa: 0.0 ± 0.0
Asn
3.073AsnAla: 3.073 ± 0.056
0.304AsnCys: 0.304 ± 0.019
2.178AsnAsp: 2.178 ± 0.059
2.662AsnGlu: 2.662 ± 0.062
1.464AsnPhe: 1.464 ± 0.049
3.134AsnGly: 3.134 ± 0.067
1.19AsnHis: 1.19 ± 0.037
2.346AsnIle: 2.346 ± 0.062
2.495AsnLys: 2.495 ± 0.063
3.505AsnLeu: 3.505 ± 0.069
0.972AsnMet: 0.972 ± 0.036
1.485AsnAsn: 1.485 ± 0.051
2.0AsnPro: 2.0 ± 0.053
2.145AsnGln: 2.145 ± 0.048
2.234AsnArg: 2.234 ± 0.053
1.882AsnSer: 1.882 ± 0.046
1.851AsnThr: 1.851 ± 0.057
2.6AsnVal: 2.6 ± 0.062
0.488AsnTrp: 0.488 ± 0.024
1.574AsnTyr: 1.574 ± 0.048
0.0AsnXaa: 0.0 ± 0.0
Pro
2.716ProAla: 2.716 ± 0.061
0.279ProCys: 0.279 ± 0.021
2.256ProAsp: 2.256 ± 0.056
3.077ProGlu: 3.077 ± 0.065
2.002ProPhe: 2.002 ± 0.055
2.476ProGly: 2.476 ± 0.052
0.825ProHis: 0.825 ± 0.037
2.844ProIle: 2.844 ± 0.065
2.2ProLys: 2.2 ± 0.051
3.75ProLeu: 3.75 ± 0.071
0.921ProMet: 0.921 ± 0.036
1.661ProAsn: 1.661 ± 0.049
1.01ProPro: 1.01 ± 0.037
1.41ProGln: 1.41 ± 0.041
1.313ProArg: 1.313 ± 0.043
2.41ProSer: 2.41 ± 0.058
1.864ProThr: 1.864 ± 0.048
2.941ProVal: 2.941 ± 0.06
0.355ProTrp: 0.355 ± 0.025
1.357ProTyr: 1.357 ± 0.04
0.0ProXaa: 0.0 ± 0.0
Gln
3.622GlnAla: 3.622 ± 0.081
0.274GlnCys: 0.274 ± 0.02
1.664GlnAsp: 1.664 ± 0.051
2.469GlnGlu: 2.469 ± 0.066
1.654GlnPhe: 1.654 ± 0.052
2.259GlnGly: 2.259 ± 0.049
0.872GlnHis: 0.872 ± 0.033
2.877GlnIle: 2.877 ± 0.065
3.007GlnLys: 3.007 ± 0.061
4.603GlnLeu: 4.603 ± 0.089
1.389GlnMet: 1.389 ± 0.048
1.749GlnAsn: 1.749 ± 0.045
1.554GlnPro: 1.554 ± 0.047
1.978GlnGln: 1.978 ± 0.06
1.948GlnArg: 1.948 ± 0.057
2.455GlnSer: 2.455 ± 0.06
2.512GlnThr: 2.512 ± 0.052
2.616GlnVal: 2.616 ± 0.06
0.471GlnTrp: 0.471 ± 0.023
1.042GlnTyr: 1.042 ± 0.035
0.0GlnXaa: 0.0 ± 0.0
Arg
3.494ArgAla: 3.494 ± 0.072
0.417ArgCys: 0.417 ± 0.023
2.515ArgAsp: 2.515 ± 0.061
3.228ArgGlu: 3.228 ± 0.072
2.309ArgPhe: 2.309 ± 0.055
2.688ArgGly: 2.688 ± 0.066
1.148ArgHis: 1.148 ± 0.045
3.633ArgIle: 3.633 ± 0.067
3.447ArgLys: 3.447 ± 0.07
5.167ArgLeu: 5.167 ± 0.082
1.545ArgMet: 1.545 ± 0.044
1.949ArgAsn: 1.949 ± 0.045
1.727ArgPro: 1.727 ± 0.046
2.118ArgGln: 2.118 ± 0.05
2.82ArgArg: 2.82 ± 0.074
2.996ArgSer: 2.996 ± 0.067
2.632ArgThr: 2.632 ± 0.059
3.254ArgVal: 3.254 ± 0.07
0.444ArgTrp: 0.444 ± 0.024
1.836ArgTyr: 1.836 ± 0.046
0.0ArgXaa: 0.0 ± 0.0
Ser
4.691SerAla: 4.691 ± 0.088
0.534SerCys: 0.534 ± 0.03
3.365SerAsp: 3.365 ± 0.08
3.956SerGlu: 3.956 ± 0.079
2.916SerPhe: 2.916 ± 0.078
5.186SerGly: 5.186 ± 0.099
1.196SerHis: 1.196 ± 0.047
4.587SerIle: 4.587 ± 0.065
3.525SerLys: 3.525 ± 0.071
6.106SerLeu: 6.106 ± 0.106
1.704SerMet: 1.704 ± 0.048
2.137SerAsn: 2.137 ± 0.054
2.014SerPro: 2.014 ± 0.067
1.952SerGln: 1.952 ± 0.053
2.704SerArg: 2.704 ± 0.058
3.792SerSer: 3.792 ± 0.092
2.91SerThr: 2.91 ± 0.061
4.563SerVal: 4.563 ± 0.078
0.626SerTrp: 0.626 ± 0.028
1.95SerTyr: 1.95 ± 0.052
0.0SerXaa: 0.0 ± 0.0
Thr
4.432ThrAla: 4.432 ± 0.083
0.479ThrCys: 0.479 ± 0.021
2.948ThrAsp: 2.948 ± 0.068
3.049ThrGlu: 3.049 ± 0.059
2.509ThrPhe: 2.509 ± 0.052
4.169ThrGly: 4.169 ± 0.081
1.251ThrHis: 1.251 ± 0.043
4.149ThrIle: 4.149 ± 0.077
2.996ThrLys: 2.996 ± 0.062
5.747ThrLeu: 5.747 ± 0.087
1.352ThrMet: 1.352 ± 0.038
2.057ThrAsn: 2.057 ± 0.054
2.246ThrPro: 2.246 ± 0.056
1.704ThrGln: 1.704 ± 0.046
2.234ThrArg: 2.234 ± 0.058
2.926ThrSer: 2.926 ± 0.073
2.742ThrThr: 2.742 ± 0.055
4.352ThrVal: 4.352 ± 0.074
0.444ThrTrp: 0.444 ± 0.025
1.816ThrTyr: 1.816 ± 0.047
0.0ThrXaa: 0.0 ± 0.0
Val
5.507ValAla: 5.507 ± 0.091
0.75ValCys: 0.75 ± 0.034
3.755ValAsp: 3.755 ± 0.069
3.456ValGlu: 3.456 ± 0.069
3.14ValPhe: 3.14 ± 0.067
4.549ValGly: 4.549 ± 0.081
1.618ValHis: 1.618 ± 0.044
5.149ValIle: 5.149 ± 0.094
4.053ValLys: 4.053 ± 0.071
7.249ValLeu: 7.249 ± 0.099
1.878ValMet: 1.878 ± 0.052
2.783ValAsn: 2.783 ± 0.06
2.924ValPro: 2.924 ± 0.057
2.562ValGln: 2.562 ± 0.051
3.339ValArg: 3.339 ± 0.068
4.634ValSer: 4.634 ± 0.081
4.269ValThr: 4.269 ± 0.079
4.861ValVal: 4.861 ± 0.087
0.578ValTrp: 0.578 ± 0.028
2.194ValTyr: 2.194 ± 0.052
0.0ValXaa: 0.0 ± 0.0
Trp
0.661TrpAla: 0.661 ± 0.029
0.071TrpCys: 0.071 ± 0.009
0.451TrpAsp: 0.451 ± 0.026
0.451TrpGlu: 0.451 ± 0.025
0.521TrpPhe: 0.521 ± 0.03
0.591TrpGly: 0.591 ± 0.029
0.242TrpHis: 0.242 ± 0.017
0.805TrpIle: 0.805 ± 0.03
0.603TrpLys: 0.603 ± 0.034
1.252TrpLeu: 1.252 ± 0.044
0.329TrpMet: 0.329 ± 0.02
0.457TrpAsn: 0.457 ± 0.025
0.318TrpPro: 0.318 ± 0.021
0.417TrpGln: 0.417 ± 0.023
0.503TrpArg: 0.503 ± 0.025
0.632TrpSer: 0.632 ± 0.03
0.507TrpThr: 0.507 ± 0.026
0.559TrpVal: 0.559 ± 0.029
0.122TrpTrp: 0.122 ± 0.013
0.298TrpTyr: 0.298 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.413TyrAla: 2.413 ± 0.055
0.332TyrCys: 0.332 ± 0.022
1.801TyrAsp: 1.801 ± 0.053
1.953TyrGlu: 1.953 ± 0.047
1.703TyrPhe: 1.703 ± 0.046
2.385TyrGly: 2.385 ± 0.05
0.864TyrHis: 0.864 ± 0.027
1.915TyrIle: 1.915 ± 0.05
1.652TyrLys: 1.652 ± 0.051
3.557TyrLeu: 3.557 ± 0.068
0.745TyrMet: 0.745 ± 0.028
1.128TyrAsn: 1.128 ± 0.043
1.442TyrPro: 1.442 ± 0.047
1.535TyrGln: 1.535 ± 0.044
1.806TyrArg: 1.806 ± 0.056
2.023TyrSer: 2.023 ± 0.055
1.736TyrThr: 1.736 ± 0.053
2.073TyrVal: 2.073 ± 0.049
0.406TyrTrp: 0.406 ± 0.027
1.281TyrTyr: 1.281 ± 0.045
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2668 proteins (789120 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski