Branchiostoma floridae (Florida lancelet) (Amphioxus)
Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Cephalochordata; Leptocardii; Amphioxiformes; Branchiostomidae; Branchiostoma
Average proteome isoelectric point is 6.46
Get precalculated fractions of proteins
Virtual 2D-PAGE plot for 28544 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C3ZYJ5|C3ZYJ5_BRAFL Uncharacterized protein OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_108653 PE=4 SV=1
MM1 pKa = 7.3ATRR4 pKa = 11.84GVEE7 pKa = 3.71QAGVLRR13 pKa = 11.84CSLLVVPGATCRR25 pKa = 11.84AAMRR29 pKa = 11.84GSFSAEE35 pKa = 3.68EE36 pKa = 3.92FSSRR40 pKa = 11.84EE41 pKa = 4.02MVRR44 pKa = 11.84SATLLVAFALLASTAHH60 pKa = 5.74GQFDD64 pKa = 4.66FGNIDD69 pKa = 3.93LDD71 pKa = 3.98GTGDD75 pKa = 3.6PFIILTRR82 pKa = 11.84PPTQAPVANPVVVQLLGQVSDD103 pKa = 5.64LIRR106 pKa = 11.84QSGDD110 pKa = 2.96PDD112 pKa = 3.55GAFFAEE118 pKa = 4.4YY119 pKa = 10.39LVGEE123 pKa = 4.74LATAGITVDD132 pKa = 4.61GLTQGIITSWSNALATEE149 pKa = 4.13EE150 pKa = 4.03NNYY153 pKa = 10.38EE154 pKa = 4.09SLALQFEE161 pKa = 5.06SVGTQLGFNAAQVAAKK177 pKa = 10.22AVADD181 pKa = 3.76YY182 pKa = 11.45NEE184 pKa = 4.32WLNVTAILRR193 pKa = 11.84ALSARR198 pKa = 11.84LQGISPDD205 pKa = 4.0NIGGVAWQISTALSSYY221 pKa = 10.65GYY223 pKa = 9.54PSDD226 pKa = 3.54NASIEE231 pKa = 4.69GIYY234 pKa = 10.31NWTVALVAAGNNTDD248 pKa = 3.37GVAMDD253 pKa = 4.37FYY255 pKa = 11.52GVAAAVGVSPGSLAQDD271 pKa = 3.99LQQLLTAALDD281 pKa = 3.79EE282 pKa = 4.87GEE284 pKa = 4.82LVYY287 pKa = 10.2FYY289 pKa = 11.26LMEE292 pKa = 4.42VKK294 pKa = 10.24QVMSNFNTSYY304 pKa = 10.41IRR306 pKa = 11.84QFSILIAQEE315 pKa = 4.29LNTLGFPLTPEE326 pKa = 3.9NSEE329 pKa = 4.36TIYY332 pKa = 9.85MWTLGLYY339 pKa = 10.16NNNDD343 pKa = 3.09GDD345 pKa = 3.47IGVYY349 pKa = 9.97INQFYY354 pKa = 10.22EE355 pKa = 4.13VAASVNKK362 pKa = 10.17SASEE366 pKa = 3.59LAKK369 pKa = 10.37IVWEE373 pKa = 4.56DD374 pKa = 3.68LTSQLQGGAAVTAHH388 pKa = 7.08LIEE391 pKa = 4.18VRR393 pKa = 11.84NLLRR397 pKa = 11.84LVEE400 pKa = 4.54FSYY403 pKa = 10.91IRR405 pKa = 11.84GLSEE409 pKa = 4.38FIAYY413 pKa = 9.78QLVPLGYY420 pKa = 9.17PVQNDD425 pKa = 3.88TFEE428 pKa = 4.79VMYY431 pKa = 8.79TWTVGMFGNDD441 pKa = 4.42DD442 pKa = 3.85ITPLIQQFYY451 pKa = 11.0DD452 pKa = 3.39VASLVNVSAADD463 pKa = 3.62LALGLKK469 pKa = 10.16DD470 pKa = 4.77ALTAQLTGGMSVVTDD485 pKa = 4.62LIEE488 pKa = 4.23VKK490 pKa = 10.49RR491 pKa = 11.84LLKK494 pKa = 10.07TSEE497 pKa = 3.76FQYY500 pKa = 10.9IDD502 pKa = 4.36DD503 pKa = 4.94LSLLITGALVPYY515 pKa = 9.83GLEE518 pKa = 4.05YY519 pKa = 11.06NNEE522 pKa = 4.43SIASIYY528 pKa = 10.62NWTAGLYY535 pKa = 10.52LSDD538 pKa = 6.08DD539 pKa = 3.26IQPYY543 pKa = 9.43VEE545 pKa = 4.09QFYY548 pKa = 10.85QVAYY552 pKa = 9.08MLNTSAPDD560 pKa = 3.44LAAGLRR566 pKa = 11.84QLLTDD571 pKa = 3.76HH572 pKa = 6.51FAEE575 pKa = 4.48VEE577 pKa = 4.27QVVMDD582 pKa = 4.55LIEE585 pKa = 4.32VKK587 pKa = 10.57RR588 pKa = 11.84LLNTVEE594 pKa = 4.23FKK596 pKa = 11.21YY597 pKa = 11.13VFGLSQLITGALGQYY612 pKa = 9.48GLEE615 pKa = 4.24YY616 pKa = 10.96NNEE619 pKa = 3.94TEE621 pKa = 4.04AFVYY625 pKa = 10.02QWTADD630 pKa = 3.64LYY632 pKa = 10.9NAQDD636 pKa = 3.55DD637 pKa = 4.34FGPQVEE643 pKa = 4.3QFYY646 pKa = 10.83QVAYY650 pKa = 8.38MLNVSVSYY658 pKa = 10.74LSNGLKK664 pKa = 10.04QLLTDD669 pKa = 4.24HH670 pKa = 6.52FAEE673 pKa = 4.54VNQVVTDD680 pKa = 4.0LLEE683 pKa = 4.26VKK685 pKa = 10.33RR686 pKa = 11.84LLGTVEE692 pKa = 3.8FQYY695 pKa = 11.34VYY697 pKa = 10.96DD698 pKa = 4.52LSLLITANLMPYY710 pKa = 9.48GLEE713 pKa = 4.05YY714 pKa = 10.93NNEE717 pKa = 4.38SIAMVYY723 pKa = 10.11GWTAGLYY730 pKa = 8.75FAQNDD735 pKa = 3.24IGPQVEE741 pKa = 4.11QFYY744 pKa = 11.07QLAYY748 pKa = 9.21MLNTSVVDD756 pKa = 3.99LAAGLKK762 pKa = 10.21QILTEE767 pKa = 4.67HH768 pKa = 6.63FDD770 pKa = 3.43KK771 pKa = 11.51VNAVVDD777 pKa = 3.77NLLQVQTLLLTLDD790 pKa = 3.65EE791 pKa = 5.0AFLPGLGQYY800 pKa = 7.78VTGQLATLGFPYY812 pKa = 10.49NDD814 pKa = 3.24EE815 pKa = 4.11TVAQLTQWLTDD826 pKa = 3.11IRR828 pKa = 11.84NGLDD832 pKa = 3.07FGAYY836 pKa = 8.39VRR838 pKa = 11.84QFYY841 pKa = 10.15EE842 pKa = 3.9VAAVVGVPEE851 pKa = 4.39VEE853 pKa = 4.23LSGNLLAGLRR863 pKa = 11.84EE864 pKa = 4.07QIALGEE870 pKa = 3.97AVYY873 pKa = 11.32YY874 pKa = 10.28DD875 pKa = 3.42IFEE878 pKa = 4.89ANQVLPQLSDD888 pKa = 2.95MDD890 pKa = 3.77VANTVNFIINFITDD904 pKa = 3.53RR905 pKa = 11.84GVMVPMDD912 pKa = 4.34LEE914 pKa = 3.87QGIRR918 pKa = 11.84NWTIALAQPSADD930 pKa = 3.72LQPIIQEE937 pKa = 4.17LYY939 pKa = 10.86GVAEE943 pKa = 5.31LINIPVRR950 pKa = 11.84DD951 pKa = 3.79MSGALVDD958 pKa = 3.91HH959 pKa = 6.72LRR961 pKa = 11.84KK962 pKa = 9.2EE963 pKa = 4.57HH964 pKa = 6.63YY965 pKa = 10.17SVPDD969 pKa = 3.8YY970 pKa = 11.29IEE972 pKa = 4.06VVALILQDD980 pKa = 3.44INVAYY985 pKa = 10.05LKK987 pKa = 10.46NVSVEE992 pKa = 3.55ITQILTGLGIPVPAPEE1008 pKa = 4.06LQMTLFEE1015 pKa = 4.33WVEE1018 pKa = 4.22NIALKK1023 pKa = 10.82PEE1025 pKa = 4.47DD1026 pKa = 4.0LPLYY1030 pKa = 9.41IDD1032 pKa = 3.45QFRR1035 pKa = 11.84EE1036 pKa = 3.7VAALVNVNIDD1046 pKa = 3.4EE1047 pKa = 4.37LAYY1050 pKa = 10.44GLLSEE1055 pKa = 4.85VEE1057 pKa = 4.33TLAEE1061 pKa = 4.15AFPDD1065 pKa = 4.06GFSPSVQNILDD1076 pKa = 3.97NLPEE1080 pKa = 4.08LMQLFSTYY1088 pKa = 10.17FPDD1091 pKa = 5.96FYY1093 pKa = 11.55ANVNNQVQQFGMAAWANVSANLTNYY1118 pKa = 9.46FNNAPPEE1125 pKa = 3.85QAMMFEE1131 pKa = 4.38NKK1133 pKa = 10.08LPEE1136 pKa = 3.94IAALLGIDD1144 pKa = 3.42IPANVLQGDD1153 pKa = 4.08ANAIIQYY1160 pKa = 9.48IQSQLPDD1167 pKa = 3.47MYY1169 pKa = 9.98ILSSAIQQALEE1180 pKa = 4.08AMTQQFPAQLVGEE1193 pKa = 4.22LLEE1196 pKa = 4.67VIFGVDD1202 pKa = 3.02IPDD1205 pKa = 4.56DD1206 pKa = 3.86FSFDD1210 pKa = 3.2PTYY1213 pKa = 10.43IVQQIPVFLGSLGVQVPSNYY1233 pKa = 9.77YY1234 pKa = 10.24DD1235 pKa = 2.82IVMAFASFEE1244 pKa = 3.99QTPEE1248 pKa = 3.26WFANNGATIVSPFGISLPDD1267 pKa = 3.7DD1268 pKa = 3.98FQPTEE1273 pKa = 4.12EE1274 pKa = 4.18YY1275 pKa = 10.79VAANLDD1281 pKa = 4.31KK1282 pKa = 10.69IIQTIANQAPQIVGVLYY1299 pKa = 8.77NTTQQIFMQVQQNIAVNSGGDD1320 pKa = 3.06VGMFIQQIIQKK1331 pKa = 9.76VLDD1334 pKa = 4.47LLPGASDD1341 pKa = 3.52YY1342 pKa = 11.48FGFYY1346 pKa = 10.48LPEE1349 pKa = 4.85DD1350 pKa = 3.81YY1351 pKa = 10.51RR1352 pKa = 11.84PEE1354 pKa = 5.1LIYY1357 pKa = 10.36FAEE1360 pKa = 4.04WGINEE1365 pKa = 4.91LFNLIPTKK1373 pKa = 10.87EE1374 pKa = 4.45EE1375 pKa = 3.87FVQMAVQPIAATLAPNDD1392 pKa = 3.31TWIAEE1397 pKa = 4.12LLEE1400 pKa = 4.52DD1401 pKa = 4.2QFSLIYY1407 pKa = 9.75DD1408 pKa = 4.14LNMVYY1413 pKa = 9.75IEE1415 pKa = 4.73HH1416 pKa = 6.58MPDD1419 pKa = 2.84ALKK1422 pKa = 10.37WIGRR1426 pKa = 11.84WLGFEE1431 pKa = 4.67LPDD1434 pKa = 4.38DD1435 pKa = 4.55FKK1437 pKa = 11.72LEE1439 pKa = 4.2PEE1441 pKa = 4.03WFIAQLPMVQAYY1453 pKa = 9.51VEE1455 pKa = 4.19QNAAMWFDD1463 pKa = 3.59QATMMFAEE1471 pKa = 4.65WSEE1474 pKa = 3.96QANMWISGFMTDD1486 pKa = 3.22SQGWNDD1492 pKa = 4.81MVNAWINDD1500 pKa = 3.67LPQTVAGLFNLDD1512 pKa = 3.84TLFMYY1517 pKa = 10.61LDD1519 pKa = 4.1PANAPEE1525 pKa = 4.52WMTMTPPPIPFDD1537 pKa = 3.62AFLTDD1542 pKa = 3.63GCVHH1546 pKa = 7.37AIAFILDD1553 pKa = 3.05VDD1555 pKa = 3.57IPEE1558 pKa = 4.77DD1559 pKa = 3.79YY1560 pKa = 10.87KK1561 pKa = 11.57VDD1563 pKa = 4.18DD1564 pKa = 4.25LSKK1567 pKa = 10.97DD1568 pKa = 3.39FNMLVNKK1575 pKa = 10.33LFMTPPDD1582 pKa = 3.8YY1583 pKa = 10.91EE1584 pKa = 4.37LSFLGEE1590 pKa = 4.45LQWQWEE1596 pKa = 4.56TFFVKK1601 pKa = 10.03PLKK1604 pKa = 10.63LDD1606 pKa = 3.5IPDD1609 pKa = 4.64DD1610 pKa = 4.16FQPSLQYY1617 pKa = 11.1LPDD1620 pKa = 2.97VMMYY1624 pKa = 10.19FGINPPDD1631 pKa = 3.67NFEE1634 pKa = 4.05PTLDD1638 pKa = 3.96FFLMWGKK1645 pKa = 10.16EE1646 pKa = 3.9FMMKK1650 pKa = 10.37LGFVFPEE1657 pKa = 3.88RR1658 pKa = 11.84DD1659 pKa = 3.22MMGARR1664 pKa = 11.84DD1665 pKa = 4.83DD1666 pKa = 4.75DD1667 pKa = 4.05ATDD1670 pKa = 3.46AQAGDD1675 pKa = 3.94VMSSDD1680 pKa = 4.76DD1681 pKa = 3.92MMMMDD1686 pKa = 4.97FAFLEE1691 pKa = 4.25YY1692 pKa = 9.48MAEE1695 pKa = 4.04FFIINQNNWPMIIKK1709 pKa = 9.62EE1710 pKa = 4.34AEE1712 pKa = 3.95TAFPMIAAHH1721 pKa = 6.36FAEE1724 pKa = 5.35AGDD1727 pKa = 4.25KK1728 pKa = 10.6IAMTFMRR1735 pKa = 11.84DD1736 pKa = 3.17VNIILQAYY1744 pKa = 7.22TDD1746 pKa = 4.17YY1747 pKa = 9.55NTKK1750 pKa = 10.15VASNFMNLYY1759 pKa = 9.78EE1760 pKa = 4.85GIYY1763 pKa = 10.59DD1764 pKa = 3.99HH1765 pKa = 6.22VTMRR1769 pKa = 11.84AQVLVKK1775 pKa = 10.57KK1776 pKa = 10.11MMMYY1780 pKa = 9.98VSHH1783 pKa = 5.98MMKK1786 pKa = 10.48LGRR1789 pKa = 11.84HH1790 pKa = 5.41NFQAMVRR1797 pKa = 11.84HH1798 pKa = 5.33LRR1800 pKa = 11.84LRR1802 pKa = 11.84LSIEE1806 pKa = 3.84LHH1808 pKa = 5.76YY1809 pKa = 10.39LAHH1812 pKa = 6.69LAEE1815 pKa = 4.32EE1816 pKa = 4.52FMNNGNNYY1824 pKa = 9.74PILQAIAQQFQQPSHH1839 pKa = 7.08PMMNILSGFLDD1850 pKa = 4.67NYY1852 pKa = 10.0FQSQNKK1858 pKa = 9.07HH1859 pKa = 6.07PIAVLHH1865 pKa = 7.24DD1866 pKa = 4.14YY1867 pKa = 10.64LHH1869 pKa = 6.5SRR1871 pKa = 11.84PSVSTSRR1878 pKa = 11.84PLIDD1882 pKa = 4.89VIANYY1887 pKa = 10.33LDD1889 pKa = 3.89GMSNRR1894 pKa = 11.84PSRR1897 pKa = 11.84PILEE1901 pKa = 4.13QIMNYY1906 pKa = 10.01FSGQQQSAWDD1916 pKa = 3.79DD1917 pKa = 3.51VADD1920 pKa = 5.23FFFGMNNNNQRR1931 pKa = 11.84PLLQQLFDD1939 pKa = 4.03NFFGSNQQQYY1949 pKa = 10.11PVIQAIADD1957 pKa = 3.66NFFGNSQQQSSPVQMLMDD1975 pKa = 3.9YY1976 pKa = 11.46LNGQSNRR1983 pKa = 11.84PNMQSIVQQLLGSSSVINQLFGNNEE2008 pKa = 4.07RR2009 pKa = 11.84PTPQQMLYY2017 pKa = 10.02EE2018 pKa = 4.86HH2019 pKa = 7.21IFGNDD2024 pKa = 3.18GQRR2027 pKa = 11.84PQASLQVLIQQMFGTNRR2044 pKa = 11.84PSPNQIMQQAVQVMFGVQQQGWNTNTFYY2072 pKa = 11.27DD2073 pKa = 5.3DD2074 pKa = 5.52DD2075 pKa = 4.48NQDD2078 pKa = 3.41PLAWLLGDD2086 pKa = 5.28DD2087 pKa = 3.47WMNWFMPEE2095 pKa = 4.71EE2096 pKa = 4.21EE2097 pKa = 5.23EE2098 pKa = 4.29NDD2100 pKa = 3.29NWFSWLFEE2108 pKa = 4.29EE2109 pKa = 5.74EE2110 pKa = 5.26DD2111 pKa = 5.11KK2112 pKa = 11.61EE2113 pKa = 5.21DD2114 pKa = 5.56DD2115 pKa = 3.9NNDD2118 pKa = 2.98WFSWLMGDD2126 pKa = 3.68EE2127 pKa = 4.75DD2128 pKa = 5.1KK2129 pKa = 11.47SSEE2132 pKa = 3.98EE2133 pKa = 4.06DD2134 pKa = 5.37KK2135 pKa = 11.13EE2136 pKa = 4.68DD2137 pKa = 3.93SWLSWLFGEE2146 pKa = 5.4EE2147 pKa = 4.24DD2148 pKa = 4.05KK2149 pKa = 11.6EE2150 pKa = 4.62DD2151 pKa = 4.04DD2152 pKa = 5.51KK2153 pKa = 12.05EE2154 pKa = 6.51DD2155 pKa = 3.92DD2156 pKa = 3.91TQDD2159 pKa = 2.73WWSSWFGGEE2168 pKa = 4.16EE2169 pKa = 4.0EE2170 pKa = 4.72EE2171 pKa = 4.81EE2172 pKa = 4.42EE2173 pKa = 4.54KK2174 pKa = 11.19PEE2176 pKa = 3.64MWYY2179 pKa = 8.08TWFFTPPMEE2188 pKa = 4.02QDD2190 pKa = 3.16KK2191 pKa = 11.21DD2192 pKa = 3.91SSEE2195 pKa = 4.24SMEE2198 pKa = 4.29NNWLSWAQPQNDD2210 pKa = 4.16DD2211 pKa = 4.55QPDD2214 pKa = 3.67TFLTWWLSDD2223 pKa = 3.54DD2224 pKa = 3.54SQMYY2228 pKa = 10.49SGFDD2232 pKa = 3.35HH2233 pKa = 6.93LVKK2236 pKa = 10.52PMQFLRR2242 pKa = 11.84GRR2244 pKa = 11.84QWGG2247 pKa = 3.48
MM1 pKa = 7.3ATRR4 pKa = 11.84GVEE7 pKa = 3.71QAGVLRR13 pKa = 11.84CSLLVVPGATCRR25 pKa = 11.84AAMRR29 pKa = 11.84GSFSAEE35 pKa = 3.68EE36 pKa = 3.92FSSRR40 pKa = 11.84EE41 pKa = 4.02MVRR44 pKa = 11.84SATLLVAFALLASTAHH60 pKa = 5.74GQFDD64 pKa = 4.66FGNIDD69 pKa = 3.93LDD71 pKa = 3.98GTGDD75 pKa = 3.6PFIILTRR82 pKa = 11.84PPTQAPVANPVVVQLLGQVSDD103 pKa = 5.64LIRR106 pKa = 11.84QSGDD110 pKa = 2.96PDD112 pKa = 3.55GAFFAEE118 pKa = 4.4YY119 pKa = 10.39LVGEE123 pKa = 4.74LATAGITVDD132 pKa = 4.61GLTQGIITSWSNALATEE149 pKa = 4.13EE150 pKa = 4.03NNYY153 pKa = 10.38EE154 pKa = 4.09SLALQFEE161 pKa = 5.06SVGTQLGFNAAQVAAKK177 pKa = 10.22AVADD181 pKa = 3.76YY182 pKa = 11.45NEE184 pKa = 4.32WLNVTAILRR193 pKa = 11.84ALSARR198 pKa = 11.84LQGISPDD205 pKa = 4.0NIGGVAWQISTALSSYY221 pKa = 10.65GYY223 pKa = 9.54PSDD226 pKa = 3.54NASIEE231 pKa = 4.69GIYY234 pKa = 10.31NWTVALVAAGNNTDD248 pKa = 3.37GVAMDD253 pKa = 4.37FYY255 pKa = 11.52GVAAAVGVSPGSLAQDD271 pKa = 3.99LQQLLTAALDD281 pKa = 3.79EE282 pKa = 4.87GEE284 pKa = 4.82LVYY287 pKa = 10.2FYY289 pKa = 11.26LMEE292 pKa = 4.42VKK294 pKa = 10.24QVMSNFNTSYY304 pKa = 10.41IRR306 pKa = 11.84QFSILIAQEE315 pKa = 4.29LNTLGFPLTPEE326 pKa = 3.9NSEE329 pKa = 4.36TIYY332 pKa = 9.85MWTLGLYY339 pKa = 10.16NNNDD343 pKa = 3.09GDD345 pKa = 3.47IGVYY349 pKa = 9.97INQFYY354 pKa = 10.22EE355 pKa = 4.13VAASVNKK362 pKa = 10.17SASEE366 pKa = 3.59LAKK369 pKa = 10.37IVWEE373 pKa = 4.56DD374 pKa = 3.68LTSQLQGGAAVTAHH388 pKa = 7.08LIEE391 pKa = 4.18VRR393 pKa = 11.84NLLRR397 pKa = 11.84LVEE400 pKa = 4.54FSYY403 pKa = 10.91IRR405 pKa = 11.84GLSEE409 pKa = 4.38FIAYY413 pKa = 9.78QLVPLGYY420 pKa = 9.17PVQNDD425 pKa = 3.88TFEE428 pKa = 4.79VMYY431 pKa = 8.79TWTVGMFGNDD441 pKa = 4.42DD442 pKa = 3.85ITPLIQQFYY451 pKa = 11.0DD452 pKa = 3.39VASLVNVSAADD463 pKa = 3.62LALGLKK469 pKa = 10.16DD470 pKa = 4.77ALTAQLTGGMSVVTDD485 pKa = 4.62LIEE488 pKa = 4.23VKK490 pKa = 10.49RR491 pKa = 11.84LLKK494 pKa = 10.07TSEE497 pKa = 3.76FQYY500 pKa = 10.9IDD502 pKa = 4.36DD503 pKa = 4.94LSLLITGALVPYY515 pKa = 9.83GLEE518 pKa = 4.05YY519 pKa = 11.06NNEE522 pKa = 4.43SIASIYY528 pKa = 10.62NWTAGLYY535 pKa = 10.52LSDD538 pKa = 6.08DD539 pKa = 3.26IQPYY543 pKa = 9.43VEE545 pKa = 4.09QFYY548 pKa = 10.85QVAYY552 pKa = 9.08MLNTSAPDD560 pKa = 3.44LAAGLRR566 pKa = 11.84QLLTDD571 pKa = 3.76HH572 pKa = 6.51FAEE575 pKa = 4.48VEE577 pKa = 4.27QVVMDD582 pKa = 4.55LIEE585 pKa = 4.32VKK587 pKa = 10.57RR588 pKa = 11.84LLNTVEE594 pKa = 4.23FKK596 pKa = 11.21YY597 pKa = 11.13VFGLSQLITGALGQYY612 pKa = 9.48GLEE615 pKa = 4.24YY616 pKa = 10.96NNEE619 pKa = 3.94TEE621 pKa = 4.04AFVYY625 pKa = 10.02QWTADD630 pKa = 3.64LYY632 pKa = 10.9NAQDD636 pKa = 3.55DD637 pKa = 4.34FGPQVEE643 pKa = 4.3QFYY646 pKa = 10.83QVAYY650 pKa = 8.38MLNVSVSYY658 pKa = 10.74LSNGLKK664 pKa = 10.04QLLTDD669 pKa = 4.24HH670 pKa = 6.52FAEE673 pKa = 4.54VNQVVTDD680 pKa = 4.0LLEE683 pKa = 4.26VKK685 pKa = 10.33RR686 pKa = 11.84LLGTVEE692 pKa = 3.8FQYY695 pKa = 11.34VYY697 pKa = 10.96DD698 pKa = 4.52LSLLITANLMPYY710 pKa = 9.48GLEE713 pKa = 4.05YY714 pKa = 10.93NNEE717 pKa = 4.38SIAMVYY723 pKa = 10.11GWTAGLYY730 pKa = 8.75FAQNDD735 pKa = 3.24IGPQVEE741 pKa = 4.11QFYY744 pKa = 11.07QLAYY748 pKa = 9.21MLNTSVVDD756 pKa = 3.99LAAGLKK762 pKa = 10.21QILTEE767 pKa = 4.67HH768 pKa = 6.63FDD770 pKa = 3.43KK771 pKa = 11.51VNAVVDD777 pKa = 3.77NLLQVQTLLLTLDD790 pKa = 3.65EE791 pKa = 5.0AFLPGLGQYY800 pKa = 7.78VTGQLATLGFPYY812 pKa = 10.49NDD814 pKa = 3.24EE815 pKa = 4.11TVAQLTQWLTDD826 pKa = 3.11IRR828 pKa = 11.84NGLDD832 pKa = 3.07FGAYY836 pKa = 8.39VRR838 pKa = 11.84QFYY841 pKa = 10.15EE842 pKa = 3.9VAAVVGVPEE851 pKa = 4.39VEE853 pKa = 4.23LSGNLLAGLRR863 pKa = 11.84EE864 pKa = 4.07QIALGEE870 pKa = 3.97AVYY873 pKa = 11.32YY874 pKa = 10.28DD875 pKa = 3.42IFEE878 pKa = 4.89ANQVLPQLSDD888 pKa = 2.95MDD890 pKa = 3.77VANTVNFIINFITDD904 pKa = 3.53RR905 pKa = 11.84GVMVPMDD912 pKa = 4.34LEE914 pKa = 3.87QGIRR918 pKa = 11.84NWTIALAQPSADD930 pKa = 3.72LQPIIQEE937 pKa = 4.17LYY939 pKa = 10.86GVAEE943 pKa = 5.31LINIPVRR950 pKa = 11.84DD951 pKa = 3.79MSGALVDD958 pKa = 3.91HH959 pKa = 6.72LRR961 pKa = 11.84KK962 pKa = 9.2EE963 pKa = 4.57HH964 pKa = 6.63YY965 pKa = 10.17SVPDD969 pKa = 3.8YY970 pKa = 11.29IEE972 pKa = 4.06VVALILQDD980 pKa = 3.44INVAYY985 pKa = 10.05LKK987 pKa = 10.46NVSVEE992 pKa = 3.55ITQILTGLGIPVPAPEE1008 pKa = 4.06LQMTLFEE1015 pKa = 4.33WVEE1018 pKa = 4.22NIALKK1023 pKa = 10.82PEE1025 pKa = 4.47DD1026 pKa = 4.0LPLYY1030 pKa = 9.41IDD1032 pKa = 3.45QFRR1035 pKa = 11.84EE1036 pKa = 3.7VAALVNVNIDD1046 pKa = 3.4EE1047 pKa = 4.37LAYY1050 pKa = 10.44GLLSEE1055 pKa = 4.85VEE1057 pKa = 4.33TLAEE1061 pKa = 4.15AFPDD1065 pKa = 4.06GFSPSVQNILDD1076 pKa = 3.97NLPEE1080 pKa = 4.08LMQLFSTYY1088 pKa = 10.17FPDD1091 pKa = 5.96FYY1093 pKa = 11.55ANVNNQVQQFGMAAWANVSANLTNYY1118 pKa = 9.46FNNAPPEE1125 pKa = 3.85QAMMFEE1131 pKa = 4.38NKK1133 pKa = 10.08LPEE1136 pKa = 3.94IAALLGIDD1144 pKa = 3.42IPANVLQGDD1153 pKa = 4.08ANAIIQYY1160 pKa = 9.48IQSQLPDD1167 pKa = 3.47MYY1169 pKa = 9.98ILSSAIQQALEE1180 pKa = 4.08AMTQQFPAQLVGEE1193 pKa = 4.22LLEE1196 pKa = 4.67VIFGVDD1202 pKa = 3.02IPDD1205 pKa = 4.56DD1206 pKa = 3.86FSFDD1210 pKa = 3.2PTYY1213 pKa = 10.43IVQQIPVFLGSLGVQVPSNYY1233 pKa = 9.77YY1234 pKa = 10.24DD1235 pKa = 2.82IVMAFASFEE1244 pKa = 3.99QTPEE1248 pKa = 3.26WFANNGATIVSPFGISLPDD1267 pKa = 3.7DD1268 pKa = 3.98FQPTEE1273 pKa = 4.12EE1274 pKa = 4.18YY1275 pKa = 10.79VAANLDD1281 pKa = 4.31KK1282 pKa = 10.69IIQTIANQAPQIVGVLYY1299 pKa = 8.77NTTQQIFMQVQQNIAVNSGGDD1320 pKa = 3.06VGMFIQQIIQKK1331 pKa = 9.76VLDD1334 pKa = 4.47LLPGASDD1341 pKa = 3.52YY1342 pKa = 11.48FGFYY1346 pKa = 10.48LPEE1349 pKa = 4.85DD1350 pKa = 3.81YY1351 pKa = 10.51RR1352 pKa = 11.84PEE1354 pKa = 5.1LIYY1357 pKa = 10.36FAEE1360 pKa = 4.04WGINEE1365 pKa = 4.91LFNLIPTKK1373 pKa = 10.87EE1374 pKa = 4.45EE1375 pKa = 3.87FVQMAVQPIAATLAPNDD1392 pKa = 3.31TWIAEE1397 pKa = 4.12LLEE1400 pKa = 4.52DD1401 pKa = 4.2QFSLIYY1407 pKa = 9.75DD1408 pKa = 4.14LNMVYY1413 pKa = 9.75IEE1415 pKa = 4.73HH1416 pKa = 6.58MPDD1419 pKa = 2.84ALKK1422 pKa = 10.37WIGRR1426 pKa = 11.84WLGFEE1431 pKa = 4.67LPDD1434 pKa = 4.38DD1435 pKa = 4.55FKK1437 pKa = 11.72LEE1439 pKa = 4.2PEE1441 pKa = 4.03WFIAQLPMVQAYY1453 pKa = 9.51VEE1455 pKa = 4.19QNAAMWFDD1463 pKa = 3.59QATMMFAEE1471 pKa = 4.65WSEE1474 pKa = 3.96QANMWISGFMTDD1486 pKa = 3.22SQGWNDD1492 pKa = 4.81MVNAWINDD1500 pKa = 3.67LPQTVAGLFNLDD1512 pKa = 3.84TLFMYY1517 pKa = 10.61LDD1519 pKa = 4.1PANAPEE1525 pKa = 4.52WMTMTPPPIPFDD1537 pKa = 3.62AFLTDD1542 pKa = 3.63GCVHH1546 pKa = 7.37AIAFILDD1553 pKa = 3.05VDD1555 pKa = 3.57IPEE1558 pKa = 4.77DD1559 pKa = 3.79YY1560 pKa = 10.87KK1561 pKa = 11.57VDD1563 pKa = 4.18DD1564 pKa = 4.25LSKK1567 pKa = 10.97DD1568 pKa = 3.39FNMLVNKK1575 pKa = 10.33LFMTPPDD1582 pKa = 3.8YY1583 pKa = 10.91EE1584 pKa = 4.37LSFLGEE1590 pKa = 4.45LQWQWEE1596 pKa = 4.56TFFVKK1601 pKa = 10.03PLKK1604 pKa = 10.63LDD1606 pKa = 3.5IPDD1609 pKa = 4.64DD1610 pKa = 4.16FQPSLQYY1617 pKa = 11.1LPDD1620 pKa = 2.97VMMYY1624 pKa = 10.19FGINPPDD1631 pKa = 3.67NFEE1634 pKa = 4.05PTLDD1638 pKa = 3.96FFLMWGKK1645 pKa = 10.16EE1646 pKa = 3.9FMMKK1650 pKa = 10.37LGFVFPEE1657 pKa = 3.88RR1658 pKa = 11.84DD1659 pKa = 3.22MMGARR1664 pKa = 11.84DD1665 pKa = 4.83DD1666 pKa = 4.75DD1667 pKa = 4.05ATDD1670 pKa = 3.46AQAGDD1675 pKa = 3.94VMSSDD1680 pKa = 4.76DD1681 pKa = 3.92MMMMDD1686 pKa = 4.97FAFLEE1691 pKa = 4.25YY1692 pKa = 9.48MAEE1695 pKa = 4.04FFIINQNNWPMIIKK1709 pKa = 9.62EE1710 pKa = 4.34AEE1712 pKa = 3.95TAFPMIAAHH1721 pKa = 6.36FAEE1724 pKa = 5.35AGDD1727 pKa = 4.25KK1728 pKa = 10.6IAMTFMRR1735 pKa = 11.84DD1736 pKa = 3.17VNIILQAYY1744 pKa = 7.22TDD1746 pKa = 4.17YY1747 pKa = 9.55NTKK1750 pKa = 10.15VASNFMNLYY1759 pKa = 9.78EE1760 pKa = 4.85GIYY1763 pKa = 10.59DD1764 pKa = 3.99HH1765 pKa = 6.22VTMRR1769 pKa = 11.84AQVLVKK1775 pKa = 10.57KK1776 pKa = 10.11MMMYY1780 pKa = 9.98VSHH1783 pKa = 5.98MMKK1786 pKa = 10.48LGRR1789 pKa = 11.84HH1790 pKa = 5.41NFQAMVRR1797 pKa = 11.84HH1798 pKa = 5.33LRR1800 pKa = 11.84LRR1802 pKa = 11.84LSIEE1806 pKa = 3.84LHH1808 pKa = 5.76YY1809 pKa = 10.39LAHH1812 pKa = 6.69LAEE1815 pKa = 4.32EE1816 pKa = 4.52FMNNGNNYY1824 pKa = 9.74PILQAIAQQFQQPSHH1839 pKa = 7.08PMMNILSGFLDD1850 pKa = 4.67NYY1852 pKa = 10.0FQSQNKK1858 pKa = 9.07HH1859 pKa = 6.07PIAVLHH1865 pKa = 7.24DD1866 pKa = 4.14YY1867 pKa = 10.64LHH1869 pKa = 6.5SRR1871 pKa = 11.84PSVSTSRR1878 pKa = 11.84PLIDD1882 pKa = 4.89VIANYY1887 pKa = 10.33LDD1889 pKa = 3.89GMSNRR1894 pKa = 11.84PSRR1897 pKa = 11.84PILEE1901 pKa = 4.13QIMNYY1906 pKa = 10.01FSGQQQSAWDD1916 pKa = 3.79DD1917 pKa = 3.51VADD1920 pKa = 5.23FFFGMNNNNQRR1931 pKa = 11.84PLLQQLFDD1939 pKa = 4.03NFFGSNQQQYY1949 pKa = 10.11PVIQAIADD1957 pKa = 3.66NFFGNSQQQSSPVQMLMDD1975 pKa = 3.9YY1976 pKa = 11.46LNGQSNRR1983 pKa = 11.84PNMQSIVQQLLGSSSVINQLFGNNEE2008 pKa = 4.07RR2009 pKa = 11.84PTPQQMLYY2017 pKa = 10.02EE2018 pKa = 4.86HH2019 pKa = 7.21IFGNDD2024 pKa = 3.18GQRR2027 pKa = 11.84PQASLQVLIQQMFGTNRR2044 pKa = 11.84PSPNQIMQQAVQVMFGVQQQGWNTNTFYY2072 pKa = 11.27DD2073 pKa = 5.3DD2074 pKa = 5.52DD2075 pKa = 4.48NQDD2078 pKa = 3.41PLAWLLGDD2086 pKa = 5.28DD2087 pKa = 3.47WMNWFMPEE2095 pKa = 4.71EE2096 pKa = 4.21EE2097 pKa = 5.23EE2098 pKa = 4.29NDD2100 pKa = 3.29NWFSWLFEE2108 pKa = 4.29EE2109 pKa = 5.74EE2110 pKa = 5.26DD2111 pKa = 5.11KK2112 pKa = 11.61EE2113 pKa = 5.21DD2114 pKa = 5.56DD2115 pKa = 3.9NNDD2118 pKa = 2.98WFSWLMGDD2126 pKa = 3.68EE2127 pKa = 4.75DD2128 pKa = 5.1KK2129 pKa = 11.47SSEE2132 pKa = 3.98EE2133 pKa = 4.06DD2134 pKa = 5.37KK2135 pKa = 11.13EE2136 pKa = 4.68DD2137 pKa = 3.93SWLSWLFGEE2146 pKa = 5.4EE2147 pKa = 4.24DD2148 pKa = 4.05KK2149 pKa = 11.6EE2150 pKa = 4.62DD2151 pKa = 4.04DD2152 pKa = 5.51KK2153 pKa = 12.05EE2154 pKa = 6.51DD2155 pKa = 3.92DD2156 pKa = 3.91TQDD2159 pKa = 2.73WWSSWFGGEE2168 pKa = 4.16EE2169 pKa = 4.0EE2170 pKa = 4.72EE2171 pKa = 4.81EE2172 pKa = 4.42EE2173 pKa = 4.54KK2174 pKa = 11.19PEE2176 pKa = 3.64MWYY2179 pKa = 8.08TWFFTPPMEE2188 pKa = 4.02QDD2190 pKa = 3.16KK2191 pKa = 11.21DD2192 pKa = 3.91SSEE2195 pKa = 4.24SMEE2198 pKa = 4.29NNWLSWAQPQNDD2210 pKa = 4.16DD2211 pKa = 4.55QPDD2214 pKa = 3.67TFLTWWLSDD2223 pKa = 3.54DD2224 pKa = 3.54SQMYY2228 pKa = 10.49SGFDD2232 pKa = 3.35HH2233 pKa = 6.93LVKK2236 pKa = 10.52PMQFLRR2242 pKa = 11.84GRR2244 pKa = 11.84QWGG2247 pKa = 3.48
Molecular weight: 254.01 kDa
Isoelectric point according different methods:
Protein with the highest isoelectric point:
>tr|C3YYI9|C3YYI9_BRAFL Protein kinase domain-containing protein (Fragment) OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_118351 PE=4 SV=1
MM1 pKa = 7.46AALVILPIMPLRR13 pKa = 11.84ILPIMPLKK21 pKa = 10.34LLPIMPLRR29 pKa = 11.84SLHH32 pKa = 6.49IMPLKK37 pKa = 10.08ILPIMPLRR45 pKa = 11.84LLPIIPQGILPIMPQRR61 pKa = 11.84ILPIMPQRR69 pKa = 11.84ILPIMPQRR77 pKa = 11.84ILSLLLSMIPHH88 pKa = 7.46LALNMNQKK96 pKa = 10.25LLTMISNTLRR106 pKa = 11.84NQPLATTPLQPRR118 pKa = 11.84HH119 pKa = 5.63TIRR122 pKa = 11.84NRR124 pKa = 11.84GIRR127 pKa = 11.84RR128 pKa = 11.84RR129 pKa = 11.84LSTVIITT136 pKa = 3.85
MM1 pKa = 7.46AALVILPIMPLRR13 pKa = 11.84ILPIMPLKK21 pKa = 10.34LLPIMPLRR29 pKa = 11.84SLHH32 pKa = 6.49IMPLKK37 pKa = 10.08ILPIMPLRR45 pKa = 11.84LLPIIPQGILPIMPQRR61 pKa = 11.84ILPIMPQRR69 pKa = 11.84ILPIMPQRR77 pKa = 11.84ILSLLLSMIPHH88 pKa = 7.46LALNMNQKK96 pKa = 10.25LLTMISNTLRR106 pKa = 11.84NQPLATTPLQPRR118 pKa = 11.84HH119 pKa = 5.63TIRR122 pKa = 11.84NRR124 pKa = 11.84GIRR127 pKa = 11.84RR128 pKa = 11.84RR129 pKa = 11.84LSTVIITT136 pKa = 3.85
Molecular weight: 15.55 kDa
Isoelectric point according different methods:
Peptides (in silico digests for buttom-up proteomics)
Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.| Try ESI |
 |
|---|
| ChTry ESI |
|
|---|
| ArgC ESI |
|
|---|
| LysN ESI |
|
|---|
| TryLysC ESI |
|
|---|
| Try MALDI |
|
|---|
| ChTry MALDI |
|
|---|
| ArgC MALDI |
|
|---|
| LysN MALDI |
|
|---|
| TryLysC MALDI |
|
|---|
| Try LTQ |
|
|---|
| ChTry LTQ |
|
|---|
| ArgC LTQ |
|
|---|
| LysN LTQ |
|
|---|
| TryLysC LTQ |
|
|---|
| Try MSlow |
|
|---|
| ChTry MSlow |
|
|---|
| ArgC MSlow |
|
|---|
| LysN MSlow |
|
|---|
| TryLysC MSlow |
|
|---|
| Try MShigh |
|
|---|
| ChTry MShigh |
|
|---|
| ArgC MShigh |
|
|---|
| LysN MShigh |
|
|---|
| TryLysC MShigh |
|
|---|
General Statistics
Number of major isoforms |
Number of additional isoforms |
Number of all proteins |
Number of amino acids |
Min. Seq. Length |
Max. Seq. Length |
Avg. Seq. Length |
Avg. Mol. Weight |
|---|---|---|---|---|---|---|---|
13248417 |
49 |
25209 |
464.1 |
51.8 |
Amino acid frequency
Ala |
Cys |
Asp |
Glu |
Phe |
Gly |
His |
Ile |
Lys |
Leu |
|---|---|---|---|---|---|---|---|---|---|
6.816 ± 0.014 | 2.499 ± 0.022 |
5.895 ± 0.022 | 6.348 ± 0.02 |
3.553 ± 0.01 | 6.824 ± 0.019 |
2.69 ± 0.009 | 4.333 ± 0.009 |
5.331 ± 0.019 | 8.628 ± 0.024 |
Met |
Asn |
Gln |
Pro |
Arg |
Ser |
Thr |
Val |
Trp |
Tyr |
|---|---|---|---|---|---|---|---|---|---|
2.434 ± 0.008 | 3.987 ± 0.012 |
5.346 ± 0.017 | 4.43 ± 0.016 |
5.706 ± 0.015 | 7.477 ± 0.016 |
6.467 ± 0.025 | 6.8 ± 0.013 |
1.318 ± 0.006 | 3.116 ± 0.011 |
Most of the basic statistics you can see at this page can be downloaded from this CSV file
See this proteome in:
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here
| Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 | Contact: Lukasz P. Kozlowski |