Ixodes scapularis (Black-legged tick) (Deer tick)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Chelicerata; Arachnida; Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Ixodinae; Ixodes

Average proteome isoelectric point is 7.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 20489 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B7PQU3|B7PQU3_IXOSC Heparan N-sulfatase putative OS=Ixodes scapularis OX=6945 GN=8052331 PE=4 SV=1
MM1 pKa = 8.04ADD3 pKa = 4.08GEE5 pKa = 4.39DD6 pKa = 3.86AAPSSDD12 pKa = 4.46SDD14 pKa = 4.73FEE16 pKa = 6.09DD17 pKa = 3.37MDD19 pKa = 5.0DD20 pKa = 5.39NEE22 pKa = 5.59GEE24 pKa = 4.29DD25 pKa = 5.08DD26 pKa = 4.08VMSDD30 pKa = 4.9AEE32 pKa = 4.42GQGAAAQEE40 pKa = 4.49GAVEE44 pKa = 4.02RR45 pKa = 11.84RR46 pKa = 11.84VYY48 pKa = 11.04VPGTTNDD55 pKa = 3.87GEE57 pKa = 4.38EE58 pKa = 4.46LEE60 pKa = 5.06CDD62 pKa = 2.92QSAYY66 pKa = 10.23VVYY69 pKa = 10.08HH70 pKa = 5.32QATTEE75 pKa = 3.97HH76 pKa = 6.65ASYY79 pKa = 10.69FHH81 pKa = 6.38MLLRR85 pKa = 11.84CRR87 pKa = 4.28

Molecular weight: 9.51 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S4LUX9|A0A1S4LUX9_IXOSC Uncharacterized protein OS=Ixodes scapularis OX=6945 PE=4 SV=1
RR1 pKa = 7.23RR2 pKa = 11.84RR3 pKa = 11.84RR4 pKa = 11.84RR5 pKa = 11.84GLARR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84LARR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84RR24 pKa = 11.84LARR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84RR32 pKa = 11.84RR33 pKa = 11.84LARR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84LARR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84WRR51 pKa = 11.84RR52 pKa = 11.84RR53 pKa = 11.84LARR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84RR62 pKa = 11.84RR63 pKa = 11.84QKK65 pKa = 10.87AKK67 pKa = 10.48SKK69 pKa = 10.7QKK71 pKa = 10.96AKK73 pKa = 10.72NKK75 pKa = 7.83QTAKK79 pKa = 10.19SKK81 pKa = 10.7QKK83 pKa = 11.05AKK85 pKa = 10.37VSEE88 pKa = 3.91SAYY91 pKa = 10.24FYY93 pKa = 11.02KK94 pKa = 9.62KK95 pKa = 10.67QKK97 pKa = 8.69MM98 pKa = 3.55

Molecular weight: 13.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

20489

0

20489

5819602

31

4588

284.0

31.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.023 ± 0.023

2.311 ± 0.017

5.145 ± 0.015

5.967 ± 0.022

3.96 ± 0.014

6.733 ± 0.027

2.638 ± 0.011

3.843 ± 0.014

4.933 ± 0.022

9.898 ± 0.028

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.195 ± 0.009

3.329 ± 0.011

5.868 ± 0.025

3.928 ± 0.017

6.708 ± 0.02

7.893 ± 0.022

5.486 ± 0.023

7.121 ± 0.019

1.254 ± 0.007

2.765 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski