Amino acid dipepetide frequency for Ixodes scapularis (Black-legged tick) (Deer tick)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	8.44AlaAla: 8.44 ± 0.065	1.701AlaCys: 1.701 ± 0.018	3.854AlaAsp: 3.854 ± 0.029	4.66AlaGlu: 4.66 ± 0.037	3.089AlaPhe: 3.089 ± 0.021	5.173AlaGly: 5.173 ± 0.039	1.855AlaHis: 1.855 ± 0.017	2.977AlaIle: 2.977 ± 0.026	3.67AlaLys: 3.67 ± 0.028	8.011AlaLeu: 8.011 ± 0.053	1.778AlaMet: 1.778 ± 0.02	2.297AlaAsn: 2.297 ± 0.021	4.649AlaPro: 4.649 ± 0.044	2.911AlaGln: 2.911 ± 0.027	4.941AlaArg: 4.941 ± 0.035	6.673AlaSer: 6.673 ± 0.043	4.715AlaThr: 4.715 ± 0.039	6.025AlaVal: 6.025 ± 0.035	0.902AlaTrp: 0.902 ± 0.014	1.908AlaTyr: 1.908 ± 0.023	0.001AlaXaa: 0.001 ± 0.0
Cys	1.568CysAla: 1.568 ± 0.018	0.681CysCys: 0.681 ± 0.015	1.101CysAsp: 1.101 ± 0.019	1.147CysGlu: 1.147 ± 0.018	1.013CysPhe: 1.013 ± 0.014	1.703CysGly: 1.703 ± 0.022	0.667CysHis: 0.667 ± 0.014	0.936CysIle: 0.936 ± 0.015	1.025CysLys: 1.025 ± 0.015	2.371CysLeu: 2.371 ± 0.023	0.447CysMet: 0.447 ± 0.009	0.775CysAsn: 0.775 ± 0.012	1.473CysPro: 1.473 ± 0.025	0.897CysGln: 0.897 ± 0.015	1.607CysArg: 1.607 ± 0.021	2.006CysSer: 2.006 ± 0.022	1.212CysThr: 1.212 ± 0.016	1.604CysVal: 1.604 ± 0.018	0.297CysTrp: 0.297 ± 0.007	0.577CysTyr: 0.577 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	4.078AspAla: 4.078 ± 0.032	1.155AspCys: 1.155 ± 0.017	3.341AspAsp: 3.341 ± 0.037	3.468AspGlu: 3.468 ± 0.032	2.111AspPhe: 2.111 ± 0.021	3.72AspGly: 3.72 ± 0.029	1.218AspHis: 1.218 ± 0.018	2.348AspIle: 2.348 ± 0.024	2.349AspLys: 2.349 ± 0.022	4.839AspLeu: 4.839 ± 0.033	1.155AspMet: 1.155 ± 0.015	1.677AspAsn: 1.677 ± 0.017	2.82AspPro: 2.82 ± 0.022	1.675AspGln: 1.675 ± 0.019	2.994AspArg: 2.994 ± 0.025	3.766AspSer: 3.766 ± 0.029	2.519AspThr: 2.519 ± 0.021	4.03AspVal: 4.03 ± 0.033	0.672AspTrp: 0.672 ± 0.013	1.51AspTyr: 1.51 ± 0.018	0.0AspXaa: 0.0 ± 0.0
Glu	5.09GluAla: 5.09 ± 0.043	1.196GluCys: 1.196 ± 0.021	3.853GluAsp: 3.853 ± 0.03	5.272GluGlu: 5.272 ± 0.049	2.0GluPhe: 2.0 ± 0.02	3.587GluGly: 3.587 ± 0.025	1.472GluHis: 1.472 ± 0.015	2.217GluIle: 2.217 ± 0.019	3.419GluLys: 3.419 ± 0.034	5.561GluLeu: 5.561 ± 0.041	1.372GluMet: 1.372 ± 0.018	2.155GluAsn: 2.155 ± 0.021	2.921GluPro: 2.921 ± 0.027	2.364GluGln: 2.364 ± 0.026	4.159GluArg: 4.159 ± 0.038	3.911GluSer: 3.911 ± 0.033	3.032GluThr: 3.032 ± 0.032	4.001GluVal: 4.001 ± 0.034	0.62GluTrp: 0.62 ± 0.011	1.471GluTyr: 1.471 ± 0.018	0.0GluXaa: 0.0 ± 0.0
Phe	2.746PheAla: 2.746 ± 0.021	1.007PheCys: 1.007 ± 0.016	1.963PheAsp: 1.963 ± 0.021	2.065PheGlu: 2.065 ± 0.023	2.029PhePhe: 2.029 ± 0.027	2.703PheGly: 2.703 ± 0.025	1.056PheHis: 1.056 ± 0.013	1.581PheIle: 1.581 ± 0.021	1.733PheLys: 1.733 ± 0.023	4.432PheLeu: 4.432 ± 0.033	0.856PheMet: 0.856 ± 0.013	1.337PheAsn: 1.337 ± 0.017	2.035PhePro: 2.035 ± 0.021	1.507PheGln: 1.507 ± 0.017	2.461PheArg: 2.461 ± 0.022	3.121PheSer: 3.121 ± 0.025	2.013PheThr: 2.013 ± 0.022	3.081PheVal: 3.081 ± 0.029	0.546PheTrp: 0.546 ± 0.01	1.328PheTyr: 1.328 ± 0.018	0.0PheXaa: 0.0 ± 0.0
Gly	5.169GlyAla: 5.169 ± 0.039	1.464GlyCys: 1.464 ± 0.018	3.577GlyAsp: 3.577 ± 0.027	3.431GlyGlu: 3.431 ± 0.029	2.649GlyPhe: 2.649 ± 0.027	6.6GlyGly: 6.6 ± 0.101	1.87GlyHis: 1.87 ± 0.021	2.685GlyIle: 2.685 ± 0.022	3.188GlyLys: 3.188 ± 0.024	5.81GlyLeu: 5.81 ± 0.036	1.373GlyMet: 1.373 ± 0.017	2.287GlyAsn: 2.287 ± 0.021	3.547GlyPro: 3.547 ± 0.044	2.38GlyGln: 2.38 ± 0.023	4.497GlyArg: 4.497 ± 0.039	5.593GlySer: 5.593 ± 0.037	3.907GlyThr: 3.907 ± 0.029	4.455GlyVal: 4.455 ± 0.031	0.796GlyTrp: 0.796 ± 0.014	2.053GlyTyr: 2.053 ± 0.026	0.001GlyXaa: 0.001 ± 0.0
His	1.778HisAla: 1.778 ± 0.02	0.719HisCys: 0.719 ± 0.012	1.142HisAsp: 1.142 ± 0.014	1.308HisGlu: 1.308 ± 0.017	1.153HisPhe: 1.153 ± 0.015	1.846HisGly: 1.846 ± 0.023	0.973HisHis: 0.973 ± 0.017	1.052HisIle: 1.052 ± 0.015	1.15HisLys: 1.15 ± 0.014	2.802HisLeu: 2.802 ± 0.023	0.616HisMet: 0.616 ± 0.01	0.885HisAsn: 0.885 ± 0.014	1.614HisPro: 1.614 ± 0.018	1.088HisGln: 1.088 ± 0.014	1.936HisArg: 1.936 ± 0.017	1.92HisSer: 1.92 ± 0.022	1.331HisThr: 1.331 ± 0.015	1.913HisVal: 1.913 ± 0.017	0.389HisTrp: 0.389 ± 0.008	0.768HisTyr: 0.768 ± 0.013	0.0HisXaa: 0.0 ± 0.0
Ile	2.866IleAla: 2.866 ± 0.026	0.906IleCys: 0.906 ± 0.012	1.91IleAsp: 1.91 ± 0.021	2.136IleGlu: 2.136 ± 0.022	1.728IlePhe: 1.728 ± 0.023	2.318IleGly: 2.318 ± 0.02	1.02IleHis: 1.02 ± 0.013	1.809IleIle: 1.809 ± 0.024	1.951IleLys: 1.951 ± 0.023	3.856IleLeu: 3.856 ± 0.033	0.926IleMet: 0.926 ± 0.014	1.425IleAsn: 1.425 ± 0.017	2.117IlePro: 2.117 ± 0.021	1.49IleGln: 1.49 ± 0.016	2.402IleArg: 2.402 ± 0.025	2.899IleSer: 2.899 ± 0.027	2.139IleThr: 2.139 ± 0.024	2.909IleVal: 2.909 ± 0.023	0.468IleTrp: 0.468 ± 0.01	1.157IleTyr: 1.157 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	3.707LysAla: 3.707 ± 0.03	1.044LysCys: 1.044 ± 0.015	2.631LysAsp: 2.631 ± 0.026	3.496LysGlu: 3.496 ± 0.034	1.665LysPhe: 1.665 ± 0.02	2.891LysGly: 2.891 ± 0.03	1.331LysHis: 1.331 ± 0.017	2.036LysIle: 2.036 ± 0.023	3.581LysLys: 3.581 ± 0.04	4.598LysLeu: 4.598 ± 0.035	1.246LysMet: 1.246 ± 0.015	1.854LysAsn: 1.854 ± 0.021	2.56LysPro: 2.56 ± 0.026	1.949LysGln: 1.949 ± 0.024	3.358LysArg: 3.358 ± 0.028	3.23LysSer: 3.23 ± 0.033	2.766LysThr: 2.766 ± 0.023	3.38LysVal: 3.38 ± 0.025	0.559LysTrp: 0.559 ± 0.009	1.453LysTyr: 1.453 ± 0.016	0.0LysXaa: 0.0 ± 0.0
Leu	7.868LeuAla: 7.868 ± 0.045	2.381LeuCys: 2.381 ± 0.021	4.992LeuAsp: 4.992 ± 0.033	6.074LeuGlu: 6.074 ± 0.047	3.785LeuPhe: 3.785 ± 0.032	6.132LeuGly: 6.132 ± 0.039	2.748LeuHis: 2.748 ± 0.024	3.198LeuIle: 3.198 ± 0.031	4.956LeuLys: 4.956 ± 0.037	10.821LeuLeu: 10.821 ± 0.062	2.047LeuMet: 2.047 ± 0.021	3.145LeuAsn: 3.145 ± 0.024	5.679LeuPro: 5.679 ± 0.037	4.455LeuGln: 4.455 ± 0.034	7.088LeuArg: 7.088 ± 0.039	7.722LeuSer: 7.722 ± 0.038	4.957LeuThr: 4.957 ± 0.032	7.019LeuVal: 7.019 ± 0.042	1.294LeuTrp: 1.294 ± 0.016	2.614LeuTyr: 2.614 ± 0.026	0.001LeuXaa: 0.001 ± 0.0
Met	2.072MetAla: 2.072 ± 0.02	0.471MetCys: 0.471 ± 0.01	1.19MetAsp: 1.19 ± 0.014	1.357MetGlu: 1.357 ± 0.017	0.856MetPhe: 0.856 ± 0.011	1.345MetGly: 1.345 ± 0.016	0.551MetHis: 0.551 ± 0.011	0.797MetIle: 0.797 ± 0.012	1.153MetLys: 1.153 ± 0.016	2.115MetLeu: 2.115 ± 0.02	0.567MetMet: 0.567 ± 0.011	0.782MetAsn: 0.782 ± 0.011	1.16MetPro: 1.16 ± 0.016	0.858MetGln: 0.858 ± 0.012	1.338MetArg: 1.338 ± 0.016	1.695MetSer: 1.695 ± 0.02	1.273MetThr: 1.273 ± 0.015	1.535MetVal: 1.535 ± 0.019	0.234MetTrp: 0.234 ± 0.006	0.603MetTyr: 0.603 ± 0.01	0.0MetXaa: 0.0 ± 0.0
Asn	2.516AsnAla: 2.516 ± 0.023	0.81AsnCys: 0.81 ± 0.013	1.529AsnAsp: 1.529 ± 0.018	1.798AsnGlu: 1.798 ± 0.02	1.437AsnPhe: 1.437 ± 0.018	2.508AsnGly: 2.508 ± 0.026	0.861AsnHis: 0.861 ± 0.015	1.667AsnIle: 1.667 ± 0.017	1.661AsnLys: 1.661 ± 0.018	3.3AsnLeu: 3.3 ± 0.028	0.806AsnMet: 0.806 ± 0.012	1.296AsnAsn: 1.296 ± 0.018	1.893AsnPro: 1.893 ± 0.02	1.159AsnGln: 1.159 ± 0.014	1.929AsnArg: 1.929 ± 0.018	2.342AsnSer: 2.342 ± 0.022	1.681AsnThr: 1.681 ± 0.017	2.579AsnVal: 2.579 ± 0.022	0.434AsnTrp: 0.434 ± 0.01	1.082AsnTyr: 1.082 ± 0.015	0.0AsnXaa: 0.0 ± 0.0
Pro	4.811ProAla: 4.811 ± 0.043	1.156ProCys: 1.156 ± 0.02	2.935ProAsp: 2.935 ± 0.025	3.691ProGlu: 3.691 ± 0.03	2.15ProPhe: 2.15 ± 0.023	4.084ProGly: 4.084 ± 0.057	1.4ProHis: 1.4 ± 0.019	1.725ProIle: 1.725 ± 0.019	2.589ProLys: 2.589 ± 0.022	5.071ProLeu: 5.071 ± 0.034	1.072ProMet: 1.072 ± 0.016	1.718ProAsn: 1.718 ± 0.018	5.46ProPro: 5.46 ± 0.076	2.39ProGln: 2.39 ± 0.026	3.88ProArg: 3.88 ± 0.034	5.173ProSer: 5.173 ± 0.039	3.208ProThr: 3.208 ± 0.028	3.97ProVal: 3.97 ± 0.03	0.733ProTrp: 0.733 ± 0.012	1.464ProTyr: 1.464 ± 0.018	0.001ProXaa: 0.001 ± 0.0
Gln	3.07GlnAla: 3.07 ± 0.026	0.887GlnCys: 0.887 ± 0.017	1.941GlnAsp: 1.941 ± 0.021	2.641GlnGlu: 2.641 ± 0.028	1.307GlnPhe: 1.307 ± 0.016	2.418GlnGly: 2.418 ± 0.023	1.132GlnHis: 1.132 ± 0.016	1.378GlnIle: 1.378 ± 0.015	1.983GlnLys: 1.983 ± 0.022	4.109GlnLeu: 4.109 ± 0.037	0.946GlnMet: 0.946 ± 0.013	1.26GlnAsn: 1.26 ± 0.014	2.226GlnPro: 2.226 ± 0.023	2.377GlnGln: 2.377 ± 0.04	2.925GlnArg: 2.925 ± 0.028	2.55GlnSer: 2.55 ± 0.022	1.975GlnThr: 1.975 ± 0.02	2.67GlnVal: 2.67 ± 0.024	0.495GlnTrp: 0.495 ± 0.011	0.983GlnTyr: 0.983 ± 0.013	0.0GlnXaa: 0.0 ± 0.0
Arg	4.854ArgAla: 4.854 ± 0.033	1.682ArgCys: 1.682 ± 0.02	3.373ArgAsp: 3.373 ± 0.028	3.864ArgGlu: 3.864 ± 0.036	2.486ArgPhe: 2.486 ± 0.021	4.175ArgGly: 4.175 ± 0.037	2.006ArgHis: 2.006 ± 0.02	2.562ArgIle: 2.562 ± 0.027	3.641ArgLys: 3.641 ± 0.031	6.582ArgLeu: 6.582 ± 0.043	1.451ArgMet: 1.451 ± 0.017	2.395ArgAsn: 2.395 ± 0.023	3.803ArgPro: 3.803 ± 0.035	2.734ArgGln: 2.734 ± 0.025	6.043ArgArg: 6.043 ± 0.047	5.087ArgSer: 5.087 ± 0.039	3.561ArgThr: 3.561 ± 0.03	4.095ArgVal: 4.095 ± 0.028	0.911ArgTrp: 0.911 ± 0.013	1.775ArgTyr: 1.775 ± 0.019	0.0ArgXaa: 0.0 ± 0.0
Ser	6.215SerAla: 6.215 ± 0.038	1.818SerCys: 1.818 ± 0.023	3.786SerAsp: 3.786 ± 0.032	4.204SerGlu: 4.204 ± 0.031	3.13SerPhe: 3.13 ± 0.025	5.448SerGly: 5.448 ± 0.034	1.91SerHis: 1.91 ± 0.02	2.778SerIle: 2.778 ± 0.026	3.57SerLys: 3.57 ± 0.032	7.767SerLeu: 7.767 ± 0.043	1.639SerMet: 1.639 ± 0.017	2.385SerAsn: 2.385 ± 0.025	5.137SerPro: 5.137 ± 0.05	2.905SerGln: 2.905 ± 0.027	5.2SerArg: 5.2 ± 0.042	8.15SerSer: 8.15 ± 0.064	4.442SerThr: 4.442 ± 0.043	5.33SerVal: 5.33 ± 0.034	1.053SerTrp: 1.053 ± 0.014	2.056SerTyr: 2.056 ± 0.02	0.0SerXaa: 0.0 ± 0.0
Thr	4.525ThrAla: 4.525 ± 0.033	1.298ThrCys: 1.298 ± 0.021	2.516ThrAsp: 2.516 ± 0.023	3.051ThrGlu: 3.051 ± 0.037	2.226ThrPhe: 2.226 ± 0.023	3.659ThrGly: 3.659 ± 0.032	1.29ThrHis: 1.29 ± 0.017	2.149ThrIle: 2.149 ± 0.025	2.492ThrLys: 2.492 ± 0.023	5.507ThrLeu: 5.507 ± 0.035	1.173ThrMet: 1.173 ± 0.017	1.706ThrAsn: 1.706 ± 0.02	3.477ThrPro: 3.477 ± 0.037	1.827ThrGln: 1.827 ± 0.019	3.197ThrArg: 3.197 ± 0.024	4.678ThrSer: 4.678 ± 0.039	3.724ThrThr: 3.724 ± 0.067	4.153ThrVal: 4.153 ± 0.034	0.719ThrTrp: 0.719 ± 0.012	1.488ThrTyr: 1.488 ± 0.019	0.0ThrXaa: 0.0 ± 0.0
Val	6.027ValAla: 6.027 ± 0.042	1.784ValCys: 1.784 ± 0.021	3.629ValAsp: 3.629 ± 0.025	3.857ValGlu: 3.857 ± 0.033	3.021ValPhe: 3.021 ± 0.025	4.246ValGly: 4.246 ± 0.032	1.936ValHis: 1.936 ± 0.019	2.799ValIle: 2.799 ± 0.024	3.166ValLys: 3.166 ± 0.026	7.352ValLeu: 7.352 ± 0.041	1.504ValMet: 1.504 ± 0.019	2.325ValAsn: 2.325 ± 0.023	4.235ValPro: 4.235 ± 0.032	2.779ValGln: 2.779 ± 0.025	4.43ValArg: 4.43 ± 0.029	5.451ValSer: 5.451 ± 0.031	4.132ValThr: 4.132 ± 0.044	5.705ValVal: 5.705 ± 0.04	0.844ValTrp: 0.844 ± 0.012	1.994ValTyr: 1.994 ± 0.019	0.001ValXaa: 0.001 ± 0.0
Trp	0.841TrpAla: 0.841 ± 0.012	0.315TrpCys: 0.315 ± 0.008	0.676TrpAsp: 0.676 ± 0.013	0.673TrpGlu: 0.673 ± 0.012	0.506TrpPhe: 0.506 ± 0.01	0.678TrpGly: 0.678 ± 0.011	0.337TrpHis: 0.337 ± 0.008	0.536TrpIle: 0.536 ± 0.01	0.765TrpLys: 0.765 ± 0.012	1.296TrpLeu: 1.296 ± 0.016	0.355TrpMet: 0.355 ± 0.008	0.519TrpAsn: 0.519 ± 0.009	0.568TrpPro: 0.568 ± 0.01	0.505TrpGln: 0.505 ± 0.011	0.876TrpArg: 0.876 ± 0.014	0.955TrpSer: 0.955 ± 0.015	0.788TrpThr: 0.788 ± 0.013	0.746TrpVal: 0.746 ± 0.013	0.223TrpTrp: 0.223 ± 0.006	0.385TrpTyr: 0.385 ± 0.009	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.987TyrAla: 1.987 ± 0.021	0.635TyrCys: 0.635 ± 0.011	1.504TyrAsp: 1.504 ± 0.019	1.481TyrGlu: 1.481 ± 0.016	1.259TyrPhe: 1.259 ± 0.016	2.1TyrGly: 2.1 ± 0.027	0.751TyrHis: 0.751 ± 0.013	1.199TyrIle: 1.199 ± 0.016	1.29TyrLys: 1.29 ± 0.017	2.775TyrLeu: 2.775 ± 0.025	0.622TyrMet: 0.622 ± 0.01	1.062TyrAsn: 1.062 ± 0.013	1.309TyrPro: 1.309 ± 0.018	1.027TyrGln: 1.027 ± 0.015	1.822TyrArg: 1.822 ± 0.02	2.002TyrSer: 2.002 ± 0.021	1.477TyrThr: 1.477 ± 0.019	2.015TyrVal: 2.015 ± 0.02	0.354TyrTrp: 0.354 ± 0.009	0.98TyrTyr: 0.98 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.001XaaMet: 0.001 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.0	0.001XaaGln: 0.001 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.006XaaXaa: 0.006 ± 0.001

Statistics based on 20489 proteins (5819602 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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