Amino acid dipepetide frequency for Mycobacterium phage Severus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.405AlaAla: 11.405 ± 1.447
0.907AlaCys: 0.907 ± 0.237
5.378AlaAsp: 5.378 ± 0.635
7.452AlaGlu: 7.452 ± 0.714
2.851AlaPhe: 2.851 ± 0.452
7.906AlaGly: 7.906 ± 0.801
2.138AlaHis: 2.138 ± 0.344
4.536AlaIle: 4.536 ± 0.572
4.86AlaLys: 4.86 ± 0.794
10.044AlaLeu: 10.044 ± 0.87
2.333AlaMet: 2.333 ± 0.354
2.786AlaAsn: 2.786 ± 0.574
6.415AlaPro: 6.415 ± 0.95
4.147AlaGln: 4.147 ± 0.559
6.286AlaArg: 6.286 ± 0.661
4.471AlaSer: 4.471 ± 0.611
6.934AlaThr: 6.934 ± 0.774
7.258AlaVal: 7.258 ± 0.791
2.462AlaTrp: 2.462 ± 0.407
2.333AlaTyr: 2.333 ± 0.39
0.0AlaXaa: 0.0 ± 0.0
Cys
0.842CysAla: 0.842 ± 0.222
0.13CysCys: 0.13 ± 0.13
0.648CysAsp: 0.648 ± 0.192
0.842CysGlu: 0.842 ± 0.203
0.194CysPhe: 0.194 ± 0.114
0.907CysGly: 0.907 ± 0.259
0.194CysHis: 0.194 ± 0.105
0.324CysIle: 0.324 ± 0.138
0.454CysLys: 0.454 ± 0.148
0.713CysLeu: 0.713 ± 0.247
0.194CysMet: 0.194 ± 0.117
0.454CysAsn: 0.454 ± 0.193
0.389CysPro: 0.389 ± 0.148
0.259CysGln: 0.259 ± 0.136
0.583CysArg: 0.583 ± 0.217
0.324CysSer: 0.324 ± 0.156
0.518CysThr: 0.518 ± 0.222
0.518CysVal: 0.518 ± 0.19
0.194CysTrp: 0.194 ± 0.104
0.454CysTyr: 0.454 ± 0.191
0.0CysXaa: 0.0 ± 0.0
Asp
7.258AspAla: 7.258 ± 0.722
0.972AspCys: 0.972 ± 0.264
3.953AspAsp: 3.953 ± 0.589
4.018AspGlu: 4.018 ± 0.56
1.62AspPhe: 1.62 ± 0.377
4.925AspGly: 4.925 ± 0.591
1.49AspHis: 1.49 ± 0.333
1.814AspIle: 1.814 ± 0.378
2.268AspLys: 2.268 ± 0.389
5.767AspLeu: 5.767 ± 0.651
1.685AspMet: 1.685 ± 0.272
2.074AspAsn: 2.074 ± 0.395
5.314AspPro: 5.314 ± 0.671
2.786AspGln: 2.786 ± 0.373
3.629AspArg: 3.629 ± 0.541
3.175AspSer: 3.175 ± 0.476
3.11AspThr: 3.11 ± 0.459
4.601AspVal: 4.601 ± 0.486
1.555AspTrp: 1.555 ± 0.284
2.333AspTyr: 2.333 ± 0.392
0.0AspXaa: 0.0 ± 0.0
Glu
7.646GluAla: 7.646 ± 0.75
0.454GluCys: 0.454 ± 0.156
4.666GluAsp: 4.666 ± 0.577
3.823GluGlu: 3.823 ± 0.5
2.851GluPhe: 2.851 ± 0.43
5.249GluGly: 5.249 ± 0.442
1.296GluHis: 1.296 ± 0.388
2.722GluIle: 2.722 ± 0.395
2.268GluLys: 2.268 ± 0.387
5.767GluLeu: 5.767 ± 0.533
1.62GluMet: 1.62 ± 0.33
2.527GluAsn: 2.527 ± 0.395
3.046GluPro: 3.046 ± 0.511
2.527GluGln: 2.527 ± 0.348
5.054GluArg: 5.054 ± 0.69
3.305GluSer: 3.305 ± 0.44
3.499GluThr: 3.499 ± 0.453
4.99GluVal: 4.99 ± 0.749
1.296GluTrp: 1.296 ± 0.227
1.685GluTyr: 1.685 ± 0.343
0.0GluXaa: 0.0 ± 0.0
Phe
3.694PheAla: 3.694 ± 0.468
0.324PheCys: 0.324 ± 0.183
3.175PheAsp: 3.175 ± 0.54
2.074PheGlu: 2.074 ± 0.419
0.065PhePhe: 0.065 ± 0.056
2.981PheGly: 2.981 ± 0.412
0.518PheHis: 0.518 ± 0.216
1.361PheIle: 1.361 ± 0.325
0.583PheLys: 0.583 ± 0.207
3.11PheLeu: 3.11 ± 0.599
0.454PheMet: 0.454 ± 0.154
1.296PheAsn: 1.296 ± 0.369
1.685PhePro: 1.685 ± 0.369
0.972PheGln: 0.972 ± 0.256
1.944PheArg: 1.944 ± 0.362
1.814PheSer: 1.814 ± 0.285
1.49PheThr: 1.49 ± 0.319
2.203PheVal: 2.203 ± 0.356
0.324PheTrp: 0.324 ± 0.164
0.713PheTyr: 0.713 ± 0.178
0.0PheXaa: 0.0 ± 0.0
Gly
5.832GlyAla: 5.832 ± 0.669
0.842GlyCys: 0.842 ± 0.195
5.249GlyAsp: 5.249 ± 0.618
4.536GlyGlu: 4.536 ± 0.514
3.046GlyPhe: 3.046 ± 0.532
6.61GlyGly: 6.61 ± 0.773
2.398GlyHis: 2.398 ± 0.432
4.212GlyIle: 4.212 ± 0.516
3.564GlyLys: 3.564 ± 0.484
7.193GlyLeu: 7.193 ± 0.788
1.75GlyMet: 1.75 ± 0.268
3.046GlyAsn: 3.046 ± 0.559
4.212GlyPro: 4.212 ± 0.513
3.175GlyGln: 3.175 ± 0.38
4.277GlyArg: 4.277 ± 0.495
4.73GlySer: 4.73 ± 0.618
5.443GlyThr: 5.443 ± 0.558
5.443GlyVal: 5.443 ± 0.553
1.62GlyTrp: 1.62 ± 0.354
3.24GlyTyr: 3.24 ± 0.497
0.0GlyXaa: 0.0 ± 0.0
His
1.62HisAla: 1.62 ± 0.339
0.13HisCys: 0.13 ± 0.092
1.426HisAsp: 1.426 ± 0.332
1.555HisGlu: 1.555 ± 0.311
0.972HisPhe: 0.972 ± 0.224
2.268HisGly: 2.268 ± 0.489
0.583HisHis: 0.583 ± 0.188
1.102HisIle: 1.102 ± 0.288
1.037HisLys: 1.037 ± 0.283
1.944HisLeu: 1.944 ± 0.423
0.13HisMet: 0.13 ± 0.082
0.324HisAsn: 0.324 ± 0.169
1.49HisPro: 1.49 ± 0.298
0.518HisGln: 0.518 ± 0.209
1.62HisArg: 1.62 ± 0.415
0.583HisSer: 0.583 ± 0.212
1.037HisThr: 1.037 ± 0.282
1.037HisVal: 1.037 ± 0.244
0.648HisTrp: 0.648 ± 0.203
0.518HisTyr: 0.518 ± 0.159
0.0HisXaa: 0.0 ± 0.0
Ile
5.184IleAla: 5.184 ± 0.632
0.454IleCys: 0.454 ± 0.167
3.24IleAsp: 3.24 ± 0.388
3.175IleGlu: 3.175 ± 0.39
0.713IlePhe: 0.713 ± 0.206
4.212IleGly: 4.212 ± 0.535
1.231IleHis: 1.231 ± 0.293
1.361IleIle: 1.361 ± 0.278
1.685IleLys: 1.685 ± 0.307
2.916IleLeu: 2.916 ± 0.51
0.648IleMet: 0.648 ± 0.196
2.009IleAsn: 2.009 ± 0.319
3.24IlePro: 3.24 ± 0.42
1.296IleGln: 1.296 ± 0.395
3.758IleArg: 3.758 ± 0.601
2.462IleSer: 2.462 ± 0.443
2.786IleThr: 2.786 ± 0.4
3.564IleVal: 3.564 ± 0.514
0.778IleTrp: 0.778 ± 0.178
1.231IleTyr: 1.231 ± 0.252
0.0IleXaa: 0.0 ± 0.0
Lys
4.277LysAla: 4.277 ± 0.417
0.259LysCys: 0.259 ± 0.148
3.24LysAsp: 3.24 ± 0.417
1.944LysGlu: 1.944 ± 0.328
0.842LysPhe: 0.842 ± 0.241
3.305LysGly: 3.305 ± 0.428
1.166LysHis: 1.166 ± 0.296
1.814LysIle: 1.814 ± 0.37
2.203LysLys: 2.203 ± 0.41
3.758LysLeu: 3.758 ± 0.487
0.907LysMet: 0.907 ± 0.2
1.361LysAsn: 1.361 ± 0.296
2.138LysPro: 2.138 ± 0.416
1.426LysGln: 1.426 ± 0.262
3.499LysArg: 3.499 ± 0.645
2.462LysSer: 2.462 ± 0.366
2.138LysThr: 2.138 ± 0.448
3.499LysVal: 3.499 ± 0.558
0.907LysTrp: 0.907 ± 0.203
0.907LysTyr: 0.907 ± 0.233
0.0LysXaa: 0.0 ± 0.0
Leu
9.007LeuAla: 9.007 ± 0.702
0.583LeuCys: 0.583 ± 0.184
5.767LeuAsp: 5.767 ± 0.541
5.897LeuGlu: 5.897 ± 0.617
2.592LeuPhe: 2.592 ± 0.417
6.934LeuGly: 6.934 ± 0.686
1.296LeuHis: 1.296 ± 0.318
3.694LeuIle: 3.694 ± 0.46
3.758LeuLys: 3.758 ± 0.533
6.48LeuLeu: 6.48 ± 0.597
2.398LeuMet: 2.398 ± 0.393
2.657LeuAsn: 2.657 ± 0.37
5.249LeuPro: 5.249 ± 0.621
2.203LeuGln: 2.203 ± 0.404
7.258LeuArg: 7.258 ± 0.677
4.86LeuSer: 4.86 ± 0.552
5.702LeuThr: 5.702 ± 0.799
4.601LeuVal: 4.601 ± 0.474
1.166LeuTrp: 1.166 ± 0.271
2.268LeuTyr: 2.268 ± 0.405
0.0LeuXaa: 0.0 ± 0.0
Met
2.268MetAla: 2.268 ± 0.322
0.0MetCys: 0.0 ± 0.0
1.361MetAsp: 1.361 ± 0.27
1.231MetGlu: 1.231 ± 0.249
0.713MetPhe: 0.713 ± 0.19
2.009MetGly: 2.009 ± 0.419
0.324MetHis: 0.324 ± 0.131
1.426MetIle: 1.426 ± 0.302
1.296MetLys: 1.296 ± 0.307
1.231MetLeu: 1.231 ± 0.271
0.454MetMet: 0.454 ± 0.161
0.842MetAsn: 0.842 ± 0.242
1.879MetPro: 1.879 ± 0.392
0.713MetGln: 0.713 ± 0.179
1.879MetArg: 1.879 ± 0.349
1.814MetSer: 1.814 ± 0.345
1.75MetThr: 1.75 ± 0.339
1.361MetVal: 1.361 ± 0.286
0.13MetTrp: 0.13 ± 0.094
0.583MetTyr: 0.583 ± 0.164
0.0MetXaa: 0.0 ± 0.0
Asn
3.758AsnAla: 3.758 ± 0.683
0.194AsnCys: 0.194 ± 0.102
2.203AsnAsp: 2.203 ± 0.282
1.555AsnGlu: 1.555 ± 0.316
0.778AsnPhe: 0.778 ± 0.211
3.499AsnGly: 3.499 ± 0.547
0.648AsnHis: 0.648 ± 0.228
2.074AsnIle: 2.074 ± 0.469
0.713AsnLys: 0.713 ± 0.232
2.527AsnLeu: 2.527 ± 0.465
0.842AsnMet: 0.842 ± 0.197
0.583AsnAsn: 0.583 ± 0.212
2.527AsnPro: 2.527 ± 0.52
0.972AsnGln: 0.972 ± 0.236
2.074AsnArg: 2.074 ± 0.368
1.49AsnSer: 1.49 ± 0.318
2.009AsnThr: 2.009 ± 0.346
2.851AsnVal: 2.851 ± 0.485
1.231AsnTrp: 1.231 ± 0.251
1.102AsnTyr: 1.102 ± 0.232
0.0AsnXaa: 0.0 ± 0.0
Pro
5.702ProAla: 5.702 ± 0.764
0.454ProCys: 0.454 ± 0.196
3.823ProAsp: 3.823 ± 0.51
5.119ProGlu: 5.119 ± 0.617
2.074ProPhe: 2.074 ± 0.299
4.86ProGly: 4.86 ± 0.617
1.296ProHis: 1.296 ± 0.24
2.398ProIle: 2.398 ± 0.334
1.944ProLys: 1.944 ± 0.385
4.536ProLeu: 4.536 ± 0.677
1.361ProMet: 1.361 ± 0.266
1.879ProAsn: 1.879 ± 0.381
3.046ProPro: 3.046 ± 0.578
1.814ProGln: 1.814 ± 0.329
3.434ProArg: 3.434 ± 0.537
3.11ProSer: 3.11 ± 0.482
3.758ProThr: 3.758 ± 0.445
4.536ProVal: 4.536 ± 0.511
1.361ProTrp: 1.361 ± 0.447
1.814ProTyr: 1.814 ± 0.379
0.0ProXaa: 0.0 ± 0.0
Gln
4.018GlnAla: 4.018 ± 0.47
0.259GlnCys: 0.259 ± 0.134
1.75GlnAsp: 1.75 ± 0.344
1.814GlnGlu: 1.814 ± 0.329
1.166GlnPhe: 1.166 ± 0.249
2.592GlnGly: 2.592 ± 0.439
0.713GlnHis: 0.713 ± 0.196
2.916GlnIle: 2.916 ± 0.492
1.231GlnLys: 1.231 ± 0.274
4.471GlnLeu: 4.471 ± 0.575
0.972GlnMet: 0.972 ± 0.217
0.907GlnAsn: 0.907 ± 0.261
1.49GlnPro: 1.49 ± 0.375
1.296GlnGln: 1.296 ± 0.291
2.916GlnArg: 2.916 ± 0.571
1.49GlnSer: 1.49 ± 0.357
1.944GlnThr: 1.944 ± 0.363
2.527GlnVal: 2.527 ± 0.363
0.583GlnTrp: 0.583 ± 0.196
0.713GlnTyr: 0.713 ± 0.237
0.0GlnXaa: 0.0 ± 0.0
Arg
7.193ArgAla: 7.193 ± 0.745
1.102ArgCys: 1.102 ± 0.362
3.694ArgAsp: 3.694 ± 0.489
5.443ArgGlu: 5.443 ± 0.609
2.462ArgPhe: 2.462 ± 0.442
4.471ArgGly: 4.471 ± 0.632
1.231ArgHis: 1.231 ± 0.222
3.37ArgIle: 3.37 ± 0.413
4.406ArgLys: 4.406 ± 0.583
4.86ArgLeu: 4.86 ± 0.563
1.879ArgMet: 1.879 ± 0.321
2.851ArgAsn: 2.851 ± 0.402
2.981ArgPro: 2.981 ± 0.507
2.851ArgGln: 2.851 ± 0.516
5.638ArgArg: 5.638 ± 0.712
3.37ArgSer: 3.37 ± 0.645
3.175ArgThr: 3.175 ± 0.431
4.925ArgVal: 4.925 ± 0.588
1.75ArgTrp: 1.75 ± 0.375
2.333ArgTyr: 2.333 ± 0.395
0.0ArgXaa: 0.0 ± 0.0
Ser
5.184SerAla: 5.184 ± 0.622
0.259SerCys: 0.259 ± 0.194
3.434SerAsp: 3.434 ± 0.419
3.758SerGlu: 3.758 ± 0.45
2.074SerPhe: 2.074 ± 0.376
4.925SerGly: 4.925 ± 0.715
0.972SerHis: 0.972 ± 0.201
2.203SerIle: 2.203 ± 0.319
2.333SerLys: 2.333 ± 0.507
3.953SerLeu: 3.953 ± 0.573
1.361SerMet: 1.361 ± 0.235
1.426SerAsn: 1.426 ± 0.242
2.592SerPro: 2.592 ± 0.398
1.75SerGln: 1.75 ± 0.314
4.471SerArg: 4.471 ± 0.591
2.398SerSer: 2.398 ± 0.474
2.786SerThr: 2.786 ± 0.636
4.147SerVal: 4.147 ± 0.521
1.361SerTrp: 1.361 ± 0.32
1.037SerTyr: 1.037 ± 0.284
0.0SerXaa: 0.0 ± 0.0
Thr
6.674ThrAla: 6.674 ± 0.676
0.518ThrCys: 0.518 ± 0.209
3.694ThrAsp: 3.694 ± 0.475
3.694ThrGlu: 3.694 ± 0.514
2.398ThrPhe: 2.398 ± 0.374
4.536ThrGly: 4.536 ± 0.464
1.166ThrHis: 1.166 ± 0.333
2.657ThrIle: 2.657 ± 0.507
2.657ThrLys: 2.657 ± 0.451
5.638ThrLeu: 5.638 ± 0.584
1.231ThrMet: 1.231 ± 0.304
1.75ThrAsn: 1.75 ± 0.372
3.694ThrPro: 3.694 ± 0.568
2.203ThrGln: 2.203 ± 0.407
3.694ThrArg: 3.694 ± 0.518
3.305ThrSer: 3.305 ± 0.47
3.499ThrThr: 3.499 ± 0.462
3.953ThrVal: 3.953 ± 0.579
0.972ThrTrp: 0.972 ± 0.28
1.426ThrTyr: 1.426 ± 0.314
0.0ThrXaa: 0.0 ± 0.0
Val
7.387ValAla: 7.387 ± 0.737
0.713ValCys: 0.713 ± 0.229
4.795ValAsp: 4.795 ± 0.607
4.86ValGlu: 4.86 ± 0.481
1.879ValPhe: 1.879 ± 0.4
4.666ValGly: 4.666 ± 0.556
0.972ValHis: 0.972 ± 0.257
3.11ValIle: 3.11 ± 0.354
3.434ValLys: 3.434 ± 0.449
5.767ValLeu: 5.767 ± 0.623
1.49ValMet: 1.49 ± 0.346
2.722ValAsn: 2.722 ± 0.576
4.277ValPro: 4.277 ± 0.638
2.527ValGln: 2.527 ± 0.453
4.277ValArg: 4.277 ± 0.484
4.471ValSer: 4.471 ± 0.563
4.536ValThr: 4.536 ± 0.601
6.674ValVal: 6.674 ± 0.694
1.296ValTrp: 1.296 ± 0.264
1.879ValTyr: 1.879 ± 0.311
0.0ValXaa: 0.0 ± 0.0
Trp
1.75TrpAla: 1.75 ± 0.337
0.194TrpCys: 0.194 ± 0.114
0.972TrpAsp: 0.972 ± 0.265
1.49TrpGlu: 1.49 ± 0.343
0.778TrpPhe: 0.778 ± 0.213
1.555TrpGly: 1.555 ± 0.302
0.518TrpHis: 0.518 ± 0.193
1.555TrpIle: 1.555 ± 0.299
0.648TrpLys: 0.648 ± 0.21
1.49TrpLeu: 1.49 ± 0.291
0.648TrpMet: 0.648 ± 0.199
0.972TrpAsn: 0.972 ± 0.249
1.102TrpPro: 1.102 ± 0.255
1.102TrpGln: 1.102 ± 0.218
1.102TrpArg: 1.102 ± 0.252
1.361TrpSer: 1.361 ± 0.306
1.231TrpThr: 1.231 ± 0.251
1.426TrpVal: 1.426 ± 0.274
0.842TrpTrp: 0.842 ± 0.252
0.259TrpTyr: 0.259 ± 0.126
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.268TyrAla: 2.268 ± 0.415
0.389TyrCys: 0.389 ± 0.19
1.879TyrAsp: 1.879 ± 0.369
2.009TyrGlu: 2.009 ± 0.361
0.713TyrPhe: 0.713 ± 0.228
2.009TyrGly: 2.009 ± 0.358
0.389TyrHis: 0.389 ± 0.183
1.296TyrIle: 1.296 ± 0.303
0.713TyrLys: 0.713 ± 0.188
2.203TyrLeu: 2.203 ± 0.374
0.842TyrMet: 0.842 ± 0.29
1.166TyrAsn: 1.166 ± 0.31
1.555TyrPro: 1.555 ± 0.224
1.231TyrGln: 1.231 ± 0.236
2.592TyrArg: 2.592 ± 0.401
1.426TyrSer: 1.426 ± 0.29
2.009TyrThr: 2.009 ± 0.396
1.62TyrVal: 1.62 ± 0.366
0.518TyrTrp: 0.518 ± 0.182
0.907TyrTyr: 0.907 ± 0.254
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 80 proteins (15433 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski