Amino acid dipepetide frequency for Mesorhizobium sp. Root172

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	17.101AlaAla: 17.101 ± 0.129	0.979AlaCys: 0.979 ± 0.024	7.137AlaAsp: 7.137 ± 0.068	7.323AlaGlu: 7.323 ± 0.076	4.773AlaPhe: 4.773 ± 0.053	11.189AlaGly: 11.189 ± 0.092	2.129AlaHis: 2.129 ± 0.035	6.873AlaIle: 6.873 ± 0.062	4.609AlaLys: 4.609 ± 0.063	12.887AlaLeu: 12.887 ± 0.089	3.585AlaMet: 3.585 ± 0.044	3.176AlaAsn: 3.176 ± 0.045	5.156AlaPro: 5.156 ± 0.059	3.489AlaGln: 3.489 ± 0.051	8.21AlaArg: 8.21 ± 0.076	6.855AlaSer: 6.855 ± 0.059	6.164AlaThr: 6.164 ± 0.075	8.885AlaVal: 8.885 ± 0.072	1.512AlaTrp: 1.512 ± 0.029	2.641AlaTyr: 2.641 ± 0.038	0.001AlaXaa: 0.001 ± 0.001
Cys	0.889CysAla: 0.889 ± 0.022	0.116CysCys: 0.116 ± 0.008	0.491CysAsp: 0.491 ± 0.017	0.4CysGlu: 0.4 ± 0.014	0.343CysPhe: 0.343 ± 0.015	0.886CysGly: 0.886 ± 0.022	0.225CysHis: 0.225 ± 0.012	0.376CysIle: 0.376 ± 0.014	0.184CysLys: 0.184 ± 0.01	0.764CysLeu: 0.764 ± 0.022	0.154CysMet: 0.154 ± 0.008	0.204CysAsn: 0.204 ± 0.011	0.393CysPro: 0.393 ± 0.016	0.215CysGln: 0.215 ± 0.011	0.563CysArg: 0.563 ± 0.017	0.461CysSer: 0.461 ± 0.015	0.425CysThr: 0.425 ± 0.014	0.587CysVal: 0.587 ± 0.02	0.123CysTrp: 0.123 ± 0.008	0.204CysTyr: 0.204 ± 0.01	0.0CysXaa: 0.0 ± 0.0
Asp	6.776AspAla: 6.776 ± 0.064	0.456AspCys: 0.456 ± 0.014	3.111AspAsp: 3.111 ± 0.05	3.302AspGlu: 3.302 ± 0.053	2.339AspPhe: 2.339 ± 0.037	5.228AspGly: 5.228 ± 0.055	1.297AspHis: 1.297 ± 0.024	3.39AspIle: 3.39 ± 0.046	2.09AspLys: 2.09 ± 0.033	5.752AspLeu: 5.752 ± 0.059	1.396AspMet: 1.396 ± 0.025	1.457AspAsn: 1.457 ± 0.034	3.374AspPro: 3.374 ± 0.042	1.736AspGln: 1.736 ± 0.034	4.305AspArg: 4.305 ± 0.048	2.127AspSer: 2.127 ± 0.034	2.58AspThr: 2.58 ± 0.055	4.123AspVal: 4.123 ± 0.053	0.975AspTrp: 0.975 ± 0.023	1.505AspTyr: 1.505 ± 0.029	0.0AspXaa: 0.0 ± 0.0
Glu	7.292GluAla: 7.292 ± 0.072	0.346GluCys: 0.346 ± 0.014	2.534GluAsp: 2.534 ± 0.037	2.836GluGlu: 2.836 ± 0.044	1.829GluPhe: 1.829 ± 0.029	3.933GluGly: 3.933 ± 0.045	1.152GluHis: 1.152 ± 0.028	3.529GluIle: 3.529 ± 0.049	2.534GluLys: 2.534 ± 0.038	5.019GluLeu: 5.019 ± 0.056	1.461GluMet: 1.461 ± 0.028	1.578GluAsn: 1.578 ± 0.03	2.723GluPro: 2.723 ± 0.044	1.977GluGln: 1.977 ± 0.033	4.532GluArg: 4.532 ± 0.063	2.103GluSer: 2.103 ± 0.041	3.37GluThr: 3.37 ± 0.04	3.555GluVal: 3.555 ± 0.046	0.7GluTrp: 0.7 ± 0.018	0.928GluTyr: 0.928 ± 0.025	0.001GluXaa: 0.001 ± 0.0
Phe	4.811PheAla: 4.811 ± 0.052	0.401PheCys: 0.401 ± 0.014	2.789PheAsp: 2.789 ± 0.041	2.195PheGlu: 2.195 ± 0.033	1.558PhePhe: 1.558 ± 0.036	3.85PheGly: 3.85 ± 0.046	0.799PheHis: 0.799 ± 0.02	1.856PheIle: 1.856 ± 0.031	1.087PheLys: 1.087 ± 0.024	3.536PheLeu: 3.536 ± 0.051	0.844PheMet: 0.844 ± 0.019	1.078PheAsn: 1.078 ± 0.024	1.671PhePro: 1.671 ± 0.029	1.068PheGln: 1.068 ± 0.023	2.331PheArg: 2.331 ± 0.031	2.448PheSer: 2.448 ± 0.041	1.93PheThr: 1.93 ± 0.033	3.074PheVal: 3.074 ± 0.043	0.603PheTrp: 0.603 ± 0.017	0.927PheTyr: 0.927 ± 0.021	0.0PheXaa: 0.0 ± 0.0
Gly	9.174GlyAla: 9.174 ± 0.077	0.795GlyCys: 0.795 ± 0.022	4.559GlyAsp: 4.559 ± 0.064	4.643GlyGlu: 4.643 ± 0.052	3.776GlyPhe: 3.776 ± 0.038	7.472GlyGly: 7.472 ± 0.1	1.926GlyHis: 1.926 ± 0.03	5.088GlyIle: 5.088 ± 0.056	4.005GlyLys: 4.005 ± 0.049	8.882GlyLeu: 8.882 ± 0.074	2.398GlyMet: 2.398 ± 0.034	2.376GlyAsn: 2.376 ± 0.053	3.47GlyPro: 3.47 ± 0.046	2.819GlyGln: 2.819 ± 0.039	5.795GlyArg: 5.795 ± 0.058	4.828GlySer: 4.828 ± 0.055	4.779GlyThr: 4.779 ± 0.067	6.162GlyVal: 6.162 ± 0.056	1.389GlyTrp: 1.389 ± 0.032	2.359GlyTyr: 2.359 ± 0.037	0.002GlyXaa: 0.002 ± 0.001
His	2.308HisAla: 2.308 ± 0.038	0.228HisCys: 0.228 ± 0.011	1.267HisAsp: 1.267 ± 0.025	1.049HisGlu: 1.049 ± 0.028	0.878HisPhe: 0.878 ± 0.02	1.988HisGly: 1.988 ± 0.03	0.56HisHis: 0.56 ± 0.019	0.968HisIle: 0.968 ± 0.024	0.566HisLys: 0.566 ± 0.019	1.971HisLeu: 1.971 ± 0.032	0.53HisMet: 0.53 ± 0.015	0.455HisAsn: 0.455 ± 0.017	1.244HisPro: 1.244 ± 0.024	0.589HisGln: 0.589 ± 0.019	1.365HisArg: 1.365 ± 0.026	0.967HisSer: 0.967 ± 0.021	0.796HisThr: 0.796 ± 0.022	1.535HisVal: 1.535 ± 0.029	0.324HisTrp: 0.324 ± 0.012	0.552HisTyr: 0.552 ± 0.016	0.0HisXaa: 0.0 ± 0.0
Ile	7.66IleAla: 7.66 ± 0.071	0.502IleCys: 0.502 ± 0.017	3.796IleAsp: 3.796 ± 0.054	3.556IleGlu: 3.556 ± 0.046	1.868IlePhe: 1.868 ± 0.037	5.338IleGly: 5.338 ± 0.054	1.023IleHis: 1.023 ± 0.024	2.579IleIle: 2.579 ± 0.04	1.673IleLys: 1.673 ± 0.034	4.56IleLeu: 4.56 ± 0.057	1.123IleMet: 1.123 ± 0.026	1.518IleAsn: 1.518 ± 0.034	2.387IlePro: 2.387 ± 0.036	1.205IleGln: 1.205 ± 0.025	3.334IleArg: 3.334 ± 0.038	3.291IleSer: 3.291 ± 0.047	2.673IleThr: 2.673 ± 0.053	4.712IleVal: 4.712 ± 0.048	0.646IleTrp: 0.646 ± 0.019	1.178IleTyr: 1.178 ± 0.024	0.0IleXaa: 0.0 ± 0.0
Lys	5.003LysAla: 5.003 ± 0.068	0.164LysCys: 0.164 ± 0.009	1.935LysAsp: 1.935 ± 0.032	1.642LysGlu: 1.642 ± 0.034	1.022LysPhe: 1.022 ± 0.026	2.958LysGly: 2.958 ± 0.041	0.624LysHis: 0.624 ± 0.019	1.914LysIle: 1.914 ± 0.035	1.603LysLys: 1.603 ± 0.039	3.667LysLeu: 3.667 ± 0.046	0.919LysMet: 0.919 ± 0.022	1.03LysAsn: 1.03 ± 0.025	2.46LysPro: 2.46 ± 0.042	1.183LysGln: 1.183 ± 0.028	2.491LysArg: 2.491 ± 0.037	2.092LysSer: 2.092 ± 0.034	2.288LysThr: 2.288 ± 0.039	2.82LysVal: 2.82 ± 0.04	0.437LysTrp: 0.437 ± 0.014	0.705LysTyr: 0.705 ± 0.022	0.0LysXaa: 0.0 ± 0.0
Leu	13.488LeuAla: 13.488 ± 0.1	0.86LeuCys: 0.86 ± 0.021	5.963LeuAsp: 5.963 ± 0.054	4.939LeuGlu: 4.939 ± 0.06	3.746LeuPhe: 3.746 ± 0.046	8.381LeuGly: 8.381 ± 0.074	1.819LeuHis: 1.819 ± 0.031	5.13LeuIle: 5.13 ± 0.063	3.931LeuLys: 3.931 ± 0.051	9.361LeuLeu: 9.361 ± 0.098	2.265LeuMet: 2.265 ± 0.037	2.503LeuAsn: 2.503 ± 0.04	5.255LeuPro: 5.255 ± 0.059	2.604LeuGln: 2.604 ± 0.036	6.255LeuArg: 6.255 ± 0.06	6.587LeuSer: 6.587 ± 0.07	5.392LeuThr: 5.392 ± 0.052	7.578LeuVal: 7.578 ± 0.066	1.121LeuTrp: 1.121 ± 0.025	2.056LeuTyr: 2.056 ± 0.039	0.003LeuXaa: 0.003 ± 0.001
Met	3.325MetAla: 3.325 ± 0.037	0.153MetCys: 0.153 ± 0.008	1.134MetAsp: 1.134 ± 0.025	1.075MetGlu: 1.075 ± 0.024	0.736MetPhe: 0.736 ± 0.021	1.718MetGly: 1.718 ± 0.034	0.456MetHis: 0.456 ± 0.015	1.422MetIle: 1.422 ± 0.026	1.087MetLys: 1.087 ± 0.025	2.574MetLeu: 2.574 ± 0.036	0.672MetMet: 0.672 ± 0.018	0.831MetAsn: 0.831 ± 0.02	1.509MetPro: 1.509 ± 0.026	0.817MetGln: 0.817 ± 0.022	1.821MetArg: 1.821 ± 0.032	1.679MetSer: 1.679 ± 0.033	1.853MetThr: 1.853 ± 0.027	1.807MetVal: 1.807 ± 0.033	0.219MetTrp: 0.219 ± 0.01	0.306MetTyr: 0.306 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	3.318AsnAla: 3.318 ± 0.043	0.26AsnCys: 0.26 ± 0.013	1.491AsnAsp: 1.491 ± 0.037	1.246AsnGlu: 1.246 ± 0.024	1.065AsnPhe: 1.065 ± 0.024	2.555AsnGly: 2.555 ± 0.047	0.521AsnHis: 0.521 ± 0.015	1.471AsnIle: 1.471 ± 0.03	0.757AsnLys: 0.757 ± 0.022	2.576AsnLeu: 2.576 ± 0.036	0.641AsnMet: 0.641 ± 0.019	0.723AsnAsn: 0.723 ± 0.023	1.893AsnPro: 1.893 ± 0.028	0.803AsnGln: 0.803 ± 0.021	1.791AsnArg: 1.791 ± 0.027	1.39AsnSer: 1.39 ± 0.031	1.298AsnThr: 1.298 ± 0.039	2.065AsnVal: 2.065 ± 0.039	0.454AsnTrp: 0.454 ± 0.016	0.714AsnTyr: 0.714 ± 0.022	0.001AsnXaa: 0.001 ± 0.0
Pro	6.329ProAla: 6.329 ± 0.063	0.284ProCys: 0.284 ± 0.012	3.52ProAsp: 3.52 ± 0.041	3.203ProGlu: 3.203 ± 0.043	2.076ProPhe: 2.076 ± 0.029	4.421ProGly: 4.421 ± 0.047	0.994ProHis: 0.994 ± 0.022	2.343ProIle: 2.343 ± 0.034	1.849ProLys: 1.849 ± 0.034	4.685ProLeu: 4.685 ± 0.052	1.192ProMet: 1.192 ± 0.025	1.357ProAsn: 1.357 ± 0.024	2.298ProPro: 2.298 ± 0.045	1.6ProGln: 1.6 ± 0.031	2.798ProArg: 2.798 ± 0.044	2.789ProSer: 2.789 ± 0.038	2.43ProThr: 2.43 ± 0.035	4.177ProVal: 4.177 ± 0.049	0.695ProTrp: 0.695 ± 0.02	1.228ProTyr: 1.228 ± 0.029	0.001ProXaa: 0.001 ± 0.001
Gln	4.001GlnAla: 4.001 ± 0.052	0.199GlnCys: 0.199 ± 0.012	1.393GlnAsp: 1.393 ± 0.028	1.386GlnGlu: 1.386 ± 0.025	1.04GlnPhe: 1.04 ± 0.023	2.369GlnGly: 2.369 ± 0.037	0.601GlnHis: 0.601 ± 0.016	1.712GlnIle: 1.712 ± 0.034	1.236GlnLys: 1.236 ± 0.029	2.742GlnLeu: 2.742 ± 0.041	0.88GlnMet: 0.88 ± 0.02	0.859GlnAsn: 0.859 ± 0.023	1.731GlnPro: 1.731 ± 0.037	1.122GlnGln: 1.122 ± 0.028	2.224GlnArg: 2.224 ± 0.032	1.739GlnSer: 1.739 ± 0.028	1.69GlnThr: 1.69 ± 0.031	2.068GlnVal: 2.068 ± 0.035	0.413GlnTrp: 0.413 ± 0.014	0.592GlnTyr: 0.592 ± 0.016	0.0GlnXaa: 0.0 ± 0.0
Arg	7.229ArgAla: 7.229 ± 0.073	0.463ArgCys: 0.463 ± 0.016	3.971ArgAsp: 3.971 ± 0.044	3.765ArgGlu: 3.765 ± 0.055	2.891ArgPhe: 2.891 ± 0.039	4.553ArgGly: 4.553 ± 0.057	1.686ArgHis: 1.686 ± 0.029	4.052ArgIle: 4.052 ± 0.048	2.541ArgLys: 2.541 ± 0.037	7.58ArgLeu: 7.58 ± 0.081	1.799ArgMet: 1.799 ± 0.029	1.957ArgAsn: 1.957 ± 0.034	3.308ArgPro: 3.308 ± 0.049	2.579ArgGln: 2.579 ± 0.041	5.23ArgArg: 5.23 ± 0.069	3.607ArgSer: 3.607 ± 0.042	3.355ArgThr: 3.355 ± 0.049	4.316ArgVal: 4.316 ± 0.048	0.943ArgTrp: 0.943 ± 0.026	1.649ArgTyr: 1.649 ± 0.029	0.001ArgXaa: 0.001 ± 0.001
Ser	6.477SerAla: 6.477 ± 0.065	0.415SerCys: 0.415 ± 0.016	3.064SerAsp: 3.064 ± 0.048	2.721SerGlu: 2.721 ± 0.044	2.354SerPhe: 2.354 ± 0.033	5.981SerGly: 5.981 ± 0.065	1.153SerHis: 1.153 ± 0.022	3.031SerIle: 3.031 ± 0.045	1.787SerLys: 1.787 ± 0.033	5.539SerLeu: 5.539 ± 0.053	1.397SerMet: 1.397 ± 0.026	1.473SerAsn: 1.473 ± 0.033	2.799SerPro: 2.799 ± 0.046	1.628SerGln: 1.628 ± 0.03	3.666SerArg: 3.666 ± 0.052	3.123SerSer: 3.123 ± 0.05	2.809SerThr: 2.809 ± 0.042	4.313SerVal: 4.313 ± 0.062	0.788SerTrp: 0.788 ± 0.02	1.287SerTyr: 1.287 ± 0.027	0.001SerXaa: 0.001 ± 0.0
Thr	6.224ThrAla: 6.224 ± 0.058	0.395ThrCys: 0.395 ± 0.015	2.798ThrAsp: 2.798 ± 0.042	2.532ThrGlu: 2.532 ± 0.036	2.104ThrPhe: 2.104 ± 0.039	5.127ThrGly: 5.127 ± 0.063	0.997ThrHis: 0.997 ± 0.021	3.24ThrIle: 3.24 ± 0.055	1.633ThrLys: 1.633 ± 0.033	5.675ThrLeu: 5.675 ± 0.053	1.301ThrMet: 1.301 ± 0.025	1.366ThrAsn: 1.366 ± 0.031	3.022ThrPro: 3.022 ± 0.041	1.342ThrGln: 1.342 ± 0.029	3.143ThrArg: 3.143 ± 0.043	3.071ThrSer: 3.071 ± 0.046	2.869ThrThr: 2.869 ± 0.051	4.459ThrVal: 4.459 ± 0.066	0.649ThrTrp: 0.649 ± 0.018	1.194ThrTyr: 1.194 ± 0.028	0.0ThrXaa: 0.0 ± 0.0
Val	9.445ValAla: 9.445 ± 0.077	0.622ValCys: 0.622 ± 0.019	4.313ValAsp: 4.313 ± 0.048	4.396ValGlu: 4.396 ± 0.058	2.937ValPhe: 2.937 ± 0.042	5.771ValGly: 5.771 ± 0.057	1.361ValHis: 1.361 ± 0.026	4.038ValIle: 4.038 ± 0.047	2.576ValLys: 2.576 ± 0.04	7.529ValLeu: 7.529 ± 0.065	1.838ValMet: 1.838 ± 0.033	1.98ValAsn: 1.98 ± 0.039	3.776ValPro: 3.776 ± 0.046	1.94ValGln: 1.94 ± 0.032	4.806ValArg: 4.806 ± 0.052	4.544ValSer: 4.544 ± 0.061	4.522ValThr: 4.522 ± 0.063	5.995ValVal: 5.995 ± 0.058	0.891ValTrp: 0.891 ± 0.021	1.493ValTyr: 1.493 ± 0.025	0.001ValXaa: 0.001 ± 0.001
Trp	1.267TrpAla: 1.267 ± 0.029	0.129TrpCys: 0.129 ± 0.009	0.604TrpAsp: 0.604 ± 0.019	0.527TrpGlu: 0.527 ± 0.017	0.569TrpPhe: 0.569 ± 0.015	0.872TrpGly: 0.872 ± 0.022	0.34TrpHis: 0.34 ± 0.014	0.681TrpIle: 0.681 ± 0.017	0.539TrpLys: 0.539 ± 0.014	1.725TrpLeu: 1.725 ± 0.032	0.359TrpMet: 0.359 ± 0.014	0.49TrpAsn: 0.49 ± 0.02	0.699TrpPro: 0.699 ± 0.018	0.561TrpGln: 0.561 ± 0.017	1.093TrpArg: 1.093 ± 0.023	0.874TrpSer: 0.874 ± 0.022	0.779TrpThr: 0.779 ± 0.017	0.824TrpVal: 0.824 ± 0.019	0.24TrpTrp: 0.24 ± 0.011	0.303TrpTyr: 0.303 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.554TyrAla: 2.554 ± 0.036	0.233TyrCys: 0.233 ± 0.011	1.447TyrAsp: 1.447 ± 0.034	1.195TyrGlu: 1.195 ± 0.024	0.953TyrPhe: 0.953 ± 0.021	2.104TyrGly: 2.104 ± 0.029	0.477TyrHis: 0.477 ± 0.015	0.928TyrIle: 0.928 ± 0.02	0.672TyrLys: 0.672 ± 0.021	2.211TyrLeu: 2.211 ± 0.034	0.439TyrMet: 0.439 ± 0.016	0.62TyrAsn: 0.62 ± 0.019	1.1TyrPro: 1.1 ± 0.023	0.718TyrGln: 0.718 ± 0.017	1.723TyrArg: 1.723 ± 0.029	1.233TyrSer: 1.233 ± 0.025	1.141TyrThr: 1.141 ± 0.026	1.718TyrVal: 1.718 ± 0.031	0.353TyrTrp: 0.353 ± 0.012	0.575TyrTyr: 0.575 ± 0.02	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.002XaaAla: 0.002 ± 0.001	0.001XaaCys: 0.001 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.002XaaLeu: 0.002 ± 0.001	0.001XaaMet: 0.001 ± 0.001	0.001XaaAsn: 0.001 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.0	0.002XaaArg: 0.002 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 6374 proteins (1980124 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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