Amino acid dipepetide frequency for Bacillus dafuensis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.398AlaAla: 5.398 ± 0.094	0.558AlaCys: 0.558 ± 0.022	3.163AlaAsp: 3.163 ± 0.045	4.463AlaGlu: 4.463 ± 0.064	3.253AlaPhe: 3.253 ± 0.062	4.895AlaGly: 4.895 ± 0.071	1.13AlaHis: 1.13 ± 0.029	6.003AlaIle: 6.003 ± 0.076	4.686AlaLys: 4.686 ± 0.072	6.709AlaLeu: 6.709 ± 0.084	1.853AlaMet: 1.853 ± 0.042	2.858AlaAsn: 2.858 ± 0.048	1.901AlaPro: 1.901 ± 0.05	1.938AlaGln: 1.938 ± 0.04	2.375AlaArg: 2.375 ± 0.036	3.894AlaSer: 3.894 ± 0.058	3.127AlaThr: 3.127 ± 0.06	4.988AlaVal: 4.988 ± 0.066	0.564AlaTrp: 0.564 ± 0.022	2.213AlaTyr: 2.213 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	0.419CysAla: 0.419 ± 0.019	0.092CysCys: 0.092 ± 0.009	0.387CysAsp: 0.387 ± 0.019	0.464CysGlu: 0.464 ± 0.02	0.337CysPhe: 0.337 ± 0.018	0.639CysGly: 0.639 ± 0.026	0.209CysHis: 0.209 ± 0.015	0.635CysIle: 0.635 ± 0.024	0.443CysLys: 0.443 ± 0.021	0.679CysLeu: 0.679 ± 0.025	0.189CysMet: 0.189 ± 0.012	0.29CysAsn: 0.29 ± 0.017	0.351CysPro: 0.351 ± 0.019	0.234CysGln: 0.234 ± 0.012	0.27CysArg: 0.27 ± 0.013	0.544CysSer: 0.544 ± 0.022	0.402CysThr: 0.402 ± 0.017	0.404CysVal: 0.404 ± 0.018	0.07CysTrp: 0.07 ± 0.008	0.254CysTyr: 0.254 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	2.99AspAla: 2.99 ± 0.048	0.378AspCys: 0.378 ± 0.018	2.361AspAsp: 2.361 ± 0.046	4.254AspGlu: 4.254 ± 0.073	2.522AspPhe: 2.522 ± 0.048	3.177AspGly: 3.177 ± 0.064	1.203AspHis: 1.203 ± 0.03	4.223AspIle: 4.223 ± 0.058	3.385AspLys: 3.385 ± 0.057	4.933AspLeu: 4.933 ± 0.065	1.247AspMet: 1.247 ± 0.034	1.829AspAsn: 1.829 ± 0.041	1.987AspPro: 1.987 ± 0.038	2.047AspGln: 2.047 ± 0.045	2.118AspArg: 2.118 ± 0.042	2.753AspSer: 2.753 ± 0.053	2.259AspThr: 2.259 ± 0.038	3.467AspVal: 3.467 ± 0.056	0.629AspTrp: 0.629 ± 0.021	2.082AspTyr: 2.082 ± 0.044	0.0AspXaa: 0.0 ± 0.0
Glu	5.047GluAla: 5.047 ± 0.073	0.434GluCys: 0.434 ± 0.019	3.722GluAsp: 3.722 ± 0.055	7.019GluGlu: 7.019 ± 0.118	2.77GluPhe: 2.77 ± 0.044	4.339GluGly: 4.339 ± 0.067	1.472GluHis: 1.472 ± 0.032	6.434GluIle: 6.434 ± 0.076	7.408GluLys: 7.408 ± 0.091	7.159GluLeu: 7.159 ± 0.095	2.433GluMet: 2.433 ± 0.048	4.116GluAsn: 4.116 ± 0.058	1.855GluPro: 1.855 ± 0.043	2.977GluGln: 2.977 ± 0.058	3.203GluArg: 3.203 ± 0.065	3.66GluSer: 3.66 ± 0.063	3.651GluThr: 3.651 ± 0.053	4.894GluVal: 4.894 ± 0.067	0.841GluTrp: 0.841 ± 0.026	2.368GluTyr: 2.368 ± 0.046	0.0GluXaa: 0.0 ± 0.0
Phe	2.971PheAla: 2.971 ± 0.054	0.372PheCys: 0.372 ± 0.018	2.348PheAsp: 2.348 ± 0.044	3.062PheGlu: 3.062 ± 0.059	2.53PhePhe: 2.53 ± 0.053	3.349PheGly: 3.349 ± 0.062	1.039PheHis: 1.039 ± 0.03	4.449PheIle: 4.449 ± 0.082	2.574PheLys: 2.574 ± 0.04	4.903PheLeu: 4.903 ± 0.075	1.206PheMet: 1.206 ± 0.031	2.134PheAsn: 2.134 ± 0.042	1.809PhePro: 1.809 ± 0.04	1.694PheGln: 1.694 ± 0.04	1.517PheArg: 1.517 ± 0.034	3.556PheSer: 3.556 ± 0.055	2.585PheThr: 2.585 ± 0.053	3.09PheVal: 3.09 ± 0.051	0.484PheTrp: 0.484 ± 0.019	1.818PheTyr: 1.818 ± 0.047	0.0PheXaa: 0.0 ± 0.0
Gly	4.485GlyAla: 4.485 ± 0.074	0.617GlyCys: 0.617 ± 0.022	3.106GlyAsp: 3.106 ± 0.052	4.429GlyGlu: 4.429 ± 0.067	3.453GlyPhe: 3.453 ± 0.052	4.482GlyGly: 4.482 ± 0.08	1.332GlyHis: 1.332 ± 0.036	6.119GlyIle: 6.119 ± 0.083	5.26GlyLys: 5.26 ± 0.069	6.387GlyLeu: 6.387 ± 0.077	2.071GlyMet: 2.071 ± 0.054	2.91GlyAsn: 2.91 ± 0.049	1.72GlyPro: 1.72 ± 0.035	2.101GlyGln: 2.101 ± 0.046	2.529GlyArg: 2.529 ± 0.053	3.92GlySer: 3.92 ± 0.05	4.039GlyThr: 4.039 ± 0.07	4.733GlyVal: 4.733 ± 0.071	0.771GlyTrp: 0.771 ± 0.028	2.739GlyTyr: 2.739 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	1.155HisAla: 1.155 ± 0.031	0.196HisCys: 0.196 ± 0.015	0.996HisAsp: 0.996 ± 0.029	1.417HisGlu: 1.417 ± 0.041	1.173HisPhe: 1.173 ± 0.031	1.307HisGly: 1.307 ± 0.037	0.664HisHis: 0.664 ± 0.028	1.545HisIle: 1.545 ± 0.038	1.057HisLys: 1.057 ± 0.028	2.124HisLeu: 2.124 ± 0.038	0.475HisMet: 0.475 ± 0.016	0.795HisAsn: 0.795 ± 0.027	1.085HisPro: 1.085 ± 0.03	0.779HisGln: 0.779 ± 0.028	0.817HisArg: 0.817 ± 0.023	1.29HisSer: 1.29 ± 0.032	0.985HisThr: 0.985 ± 0.028	1.332HisVal: 1.332 ± 0.036	0.208HisTrp: 0.208 ± 0.015	0.901HisTyr: 0.901 ± 0.029	0.0HisXaa: 0.0 ± 0.0
Ile	5.946IleAla: 5.946 ± 0.073	0.682IleCys: 0.682 ± 0.025	4.512IleAsp: 4.512 ± 0.056	6.382IleGlu: 6.382 ± 0.076	3.899IlePhe: 3.899 ± 0.075	6.236IleGly: 6.236 ± 0.075	1.811IleHis: 1.811 ± 0.034	7.073IleIle: 7.073 ± 0.096	5.377IleLys: 5.377 ± 0.074	7.941IleLeu: 7.941 ± 0.092	2.04IleMet: 2.04 ± 0.041	3.855IleAsn: 3.855 ± 0.058	3.607IlePro: 3.607 ± 0.056	3.142IleGln: 3.142 ± 0.054	3.295IleArg: 3.295 ± 0.046	5.718IleSer: 5.718 ± 0.076	4.515IleThr: 4.515 ± 0.06	5.639IleVal: 5.639 ± 0.068	0.713IleTrp: 0.713 ± 0.025	2.574IleTyr: 2.574 ± 0.046	0.0IleXaa: 0.0 ± 0.0
Lys	4.838LysAla: 4.838 ± 0.068	0.4LysCys: 0.4 ± 0.017	4.172LysAsp: 4.172 ± 0.056	7.353LysGlu: 7.353 ± 0.095	2.168LysPhe: 2.168 ± 0.04	4.872LysGly: 4.872 ± 0.067	1.3LysHis: 1.3 ± 0.034	5.389LysIle: 5.389 ± 0.07	6.586LysLys: 6.586 ± 0.085	6.279LysLeu: 6.279 ± 0.071	2.425LysMet: 2.425 ± 0.045	3.904LysAsn: 3.904 ± 0.069	2.34LysPro: 2.34 ± 0.041	2.954LysGln: 2.954 ± 0.047	3.161LysArg: 3.161 ± 0.058	3.926LysSer: 3.926 ± 0.054	3.689LysThr: 3.689 ± 0.055	4.812LysVal: 4.812 ± 0.075	0.886LysTrp: 0.886 ± 0.03	2.381LysTyr: 2.381 ± 0.05	0.0LysXaa: 0.0 ± 0.0
Leu	6.861LeuAla: 6.861 ± 0.07	0.683LeuCys: 0.683 ± 0.028	4.683LeuAsp: 4.683 ± 0.056	6.761LeuGlu: 6.761 ± 0.09	5.016LeuPhe: 5.016 ± 0.087	6.238LeuGly: 6.238 ± 0.081	1.868LeuHis: 1.868 ± 0.036	7.875LeuIle: 7.875 ± 0.099	7.1LeuLys: 7.1 ± 0.082	10.184LeuLeu: 10.184 ± 0.115	2.419LeuMet: 2.419 ± 0.048	4.525LeuAsn: 4.525 ± 0.07	3.91LeuPro: 3.91 ± 0.063	3.473LeuGln: 3.473 ± 0.066	3.473LeuArg: 3.473 ± 0.058	6.98LeuSer: 6.98 ± 0.079	5.657LeuThr: 5.657 ± 0.065	5.791LeuVal: 5.791 ± 0.069	0.793LeuTrp: 0.793 ± 0.026	3.127LeuTyr: 3.127 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	1.927MetAla: 1.927 ± 0.041	0.141MetCys: 0.141 ± 0.01	1.429MetAsp: 1.429 ± 0.037	2.084MetGlu: 2.084 ± 0.035	1.065MetPhe: 1.065 ± 0.027	1.686MetGly: 1.686 ± 0.043	0.428MetHis: 0.428 ± 0.019	2.344MetIle: 2.344 ± 0.045	2.689MetLys: 2.689 ± 0.045	2.533MetLeu: 2.533 ± 0.05	0.813MetMet: 0.813 ± 0.032	1.688MetAsn: 1.688 ± 0.037	1.042MetPro: 1.042 ± 0.041	0.833MetGln: 0.833 ± 0.023	1.015MetArg: 1.015 ± 0.03	1.619MetSer: 1.619 ± 0.037	1.581MetThr: 1.581 ± 0.035	1.635MetVal: 1.635 ± 0.038	0.174MetTrp: 0.174 ± 0.011	0.778MetTyr: 0.778 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	2.767AsnAla: 2.767 ± 0.043	0.349AsnCys: 0.349 ± 0.019	2.358AsnAsp: 2.358 ± 0.045	3.977AsnGlu: 3.977 ± 0.048	1.742AsnPhe: 1.742 ± 0.039	3.578AsnGly: 3.578 ± 0.061	1.115AsnHis: 1.115 ± 0.031	3.946AsnIle: 3.946 ± 0.056	3.415AsnLys: 3.415 ± 0.061	4.041AsnLeu: 4.041 ± 0.059	1.329AsnMet: 1.329 ± 0.03	2.33AsnAsn: 2.33 ± 0.05	2.22AsnPro: 2.22 ± 0.042	2.043AsnGln: 2.043 ± 0.046	2.073AsnArg: 2.073 ± 0.04	2.635AsnSer: 2.635 ± 0.043	2.25AsnThr: 2.25 ± 0.037	2.963AsnVal: 2.963 ± 0.049	0.55AsnTrp: 0.55 ± 0.021	1.541AsnTyr: 1.541 ± 0.035	0.0AsnXaa: 0.0 ± 0.0
Pro	2.204ProAla: 2.204 ± 0.048	0.209ProCys: 0.209 ± 0.015	1.952ProAsp: 1.952 ± 0.04	2.862ProGlu: 2.862 ± 0.055	2.142ProPhe: 2.142 ± 0.041	2.3ProGly: 2.3 ± 0.056	0.707ProHis: 0.707 ± 0.023	3.184ProIle: 3.184 ± 0.053	2.306ProLys: 2.306 ± 0.041	3.523ProLeu: 3.523 ± 0.061	0.816ProMet: 0.816 ± 0.027	1.801ProAsn: 1.801 ± 0.043	1.039ProPro: 1.039 ± 0.027	1.122ProGln: 1.122 ± 0.031	1.063ProArg: 1.063 ± 0.031	2.396ProSer: 2.396 ± 0.042	1.9ProThr: 1.9 ± 0.041	2.633ProVal: 2.633 ± 0.046	0.334ProTrp: 0.334 ± 0.017	1.406ProTyr: 1.406 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	2.425GlnAla: 2.425 ± 0.047	0.204GlnCys: 0.204 ± 0.014	1.606GlnAsp: 1.606 ± 0.039	2.699GlnGlu: 2.699 ± 0.053	1.67GlnPhe: 1.67 ± 0.039	1.944GlnGly: 1.944 ± 0.041	0.68GlnHis: 0.68 ± 0.027	2.824GlnIle: 2.824 ± 0.053	2.793GlnLys: 2.793 ± 0.05	3.777GlnLeu: 3.777 ± 0.063	1.104GlnMet: 1.104 ± 0.028	1.75GlnAsn: 1.75 ± 0.041	1.134GlnPro: 1.134 ± 0.035	1.482GlnGln: 1.482 ± 0.043	1.311GlnArg: 1.311 ± 0.033	2.203GlnSer: 2.203 ± 0.044	1.858GlnThr: 1.858 ± 0.038	2.227GlnVal: 2.227 ± 0.044	0.334GlnTrp: 0.334 ± 0.015	1.329GlnTyr: 1.329 ± 0.035	0.0GlnXaa: 0.0 ± 0.0
Arg	2.219ArgAla: 2.219 ± 0.045	0.245ArgCys: 0.245 ± 0.015	1.851ArgAsp: 1.851 ± 0.042	3.035ArgGlu: 3.035 ± 0.06	1.889ArgPhe: 1.889 ± 0.042	2.187ArgGly: 2.187 ± 0.047	0.735ArgHis: 0.735 ± 0.026	3.175ArgIle: 3.175 ± 0.047	3.289ArgLys: 3.289 ± 0.054	3.807ArgLeu: 3.807 ± 0.049	1.168ArgMet: 1.168 ± 0.029	1.978ArgAsn: 1.978 ± 0.037	1.281ArgPro: 1.281 ± 0.037	1.293ArgGln: 1.293 ± 0.035	1.727ArgArg: 1.727 ± 0.045	2.184ArgSer: 2.184 ± 0.04	1.873ArgThr: 1.873 ± 0.035	2.456ArgVal: 2.456 ± 0.045	0.398ArgTrp: 0.398 ± 0.017	1.401ArgTyr: 1.401 ± 0.033	0.0ArgXaa: 0.0 ± 0.0
Ser	3.623SerAla: 3.623 ± 0.062	0.455SerCys: 0.455 ± 0.018	2.808SerAsp: 2.808 ± 0.051	4.247SerGlu: 4.247 ± 0.062	3.63SerPhe: 3.63 ± 0.054	4.423SerGly: 4.423 ± 0.059	1.228SerHis: 1.228 ± 0.03	5.727SerIle: 5.727 ± 0.07	4.254SerLys: 4.254 ± 0.057	6.48SerLeu: 6.48 ± 0.074	1.713SerMet: 1.713 ± 0.042	2.82SerAsn: 2.82 ± 0.05	2.23SerPro: 2.23 ± 0.039	1.943SerGln: 1.943 ± 0.036	2.206SerArg: 2.206 ± 0.045	4.251SerSer: 4.251 ± 0.088	3.177SerThr: 3.177 ± 0.05	4.018SerVal: 4.018 ± 0.059	0.677SerTrp: 0.677 ± 0.019	2.289SerTyr: 2.289 ± 0.047	0.0SerXaa: 0.0 ± 0.0
Thr	3.769ThrAla: 3.769 ± 0.061	0.345ThrCys: 0.345 ± 0.018	2.585ThrAsp: 2.585 ± 0.046	3.466ThrGlu: 3.466 ± 0.05	2.642ThrPhe: 2.642 ± 0.05	4.155ThrGly: 4.155 ± 0.057	1.014ThrHis: 1.014 ± 0.028	4.695ThrIle: 4.695 ± 0.06	3.343ThrLys: 3.343 ± 0.056	4.909ThrLeu: 4.909 ± 0.068	1.262ThrMet: 1.262 ± 0.031	2.497ThrAsn: 2.497 ± 0.05	2.189ThrPro: 2.189 ± 0.045	1.336ThrGln: 1.336 ± 0.034	1.692ThrArg: 1.692 ± 0.04	3.277ThrSer: 3.277 ± 0.059	2.72ThrThr: 2.72 ± 0.048	4.04ThrVal: 4.04 ± 0.064	0.49ThrTrp: 0.49 ± 0.019	1.819ThrTyr: 1.819 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	4.385ValAla: 4.385 ± 0.072	0.556ValCys: 0.556 ± 0.019	3.421ValAsp: 3.421 ± 0.058	4.549ValGlu: 4.549 ± 0.067	3.178ValPhe: 3.178 ± 0.052	4.258ValGly: 4.258 ± 0.071	1.299ValHis: 1.299 ± 0.032	5.818ValIle: 5.818 ± 0.072	4.84ValLys: 4.84 ± 0.069	6.549ValLeu: 6.549 ± 0.071	1.729ValMet: 1.729 ± 0.035	3.129ValAsn: 3.129 ± 0.048	2.522ValPro: 2.522 ± 0.042	2.233ValGln: 2.233 ± 0.043	2.398ValArg: 2.398 ± 0.049	4.486ValSer: 4.486 ± 0.059	3.706ValThr: 3.706 ± 0.065	4.449ValVal: 4.449 ± 0.067	0.597ValTrp: 0.597 ± 0.025	2.155ValTyr: 2.155 ± 0.048	0.0ValXaa: 0.0 ± 0.0
Trp	0.587TrpAla: 0.587 ± 0.022	0.077TrpCys: 0.077 ± 0.007	0.516TrpAsp: 0.516 ± 0.021	0.701TrpGlu: 0.701 ± 0.027	0.492TrpPhe: 0.492 ± 0.021	0.704TrpGly: 0.704 ± 0.026	0.198TrpHis: 0.198 ± 0.014	0.873TrpIle: 0.873 ± 0.027	0.773TrpLys: 0.773 ± 0.022	1.129TrpLeu: 1.129 ± 0.028	0.314TrpMet: 0.314 ± 0.015	0.545TrpAsn: 0.545 ± 0.022	0.249TrpPro: 0.249 ± 0.015	0.345TrpGln: 0.345 ± 0.015	0.368TrpArg: 0.368 ± 0.018	0.572TrpSer: 0.572 ± 0.021	0.5TrpThr: 0.5 ± 0.022	0.626TrpVal: 0.626 ± 0.023	0.134TrpTrp: 0.134 ± 0.011	0.342TrpTyr: 0.342 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.954TyrAla: 1.954 ± 0.041	0.318TyrCys: 0.318 ± 0.016	1.87TyrAsp: 1.87 ± 0.038	2.576TyrGlu: 2.576 ± 0.05	1.919TyrPhe: 1.919 ± 0.047	2.433TyrGly: 2.433 ± 0.042	0.87TyrHis: 0.87 ± 0.029	2.626TyrIle: 2.626 ± 0.053	2.259TyrLys: 2.259 ± 0.048	3.368TyrLeu: 3.368 ± 0.051	0.898TyrMet: 0.898 ± 0.026	1.466TyrAsn: 1.466 ± 0.033	1.429TyrPro: 1.429 ± 0.035	1.383TyrGln: 1.383 ± 0.033	1.582TyrArg: 1.582 ± 0.04	2.337TyrSer: 2.337 ± 0.045	1.77TyrThr: 1.77 ± 0.034	2.058TyrVal: 2.058 ± 0.038	0.4TyrTrp: 0.4 ± 0.018	1.437TyrTyr: 1.437 ± 0.038	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4401 proteins (1318923 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

---