Amino acid dipepetide frequency for Mycobacterium phage SiSi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.019AlaAla: 14.019 ± 1.532
0.946AlaCys: 0.946 ± 0.232
7.232AlaAsp: 7.232 ± 0.673
7.287AlaGlu: 7.287 ± 0.756
2.726AlaPhe: 2.726 ± 0.375
10.18AlaGly: 10.18 ± 1.093
2.392AlaHis: 2.392 ± 0.428
4.395AlaIle: 4.395 ± 0.523
3.838AlaLys: 3.838 ± 0.496
7.955AlaLeu: 7.955 ± 0.727
2.392AlaMet: 2.392 ± 0.392
2.726AlaAsn: 2.726 ± 0.319
5.34AlaPro: 5.34 ± 0.663
3.505AlaGln: 3.505 ± 0.515
6.954AlaArg: 6.954 ± 0.764
5.452AlaSer: 5.452 ± 0.609
6.62AlaThr: 6.62 ± 0.519
6.898AlaVal: 6.898 ± 0.627
2.448AlaTrp: 2.448 ± 0.422
2.281AlaTyr: 2.281 ± 0.325
0.0AlaXaa: 0.0 ± 0.0
Cys
0.779CysAla: 0.779 ± 0.232
0.0CysCys: 0.0 ± 0.0
1.335CysAsp: 1.335 ± 0.377
1.113CysGlu: 1.113 ± 0.27
0.167CysPhe: 0.167 ± 0.102
1.558CysGly: 1.558 ± 0.314
0.334CysHis: 0.334 ± 0.14
0.278CysIle: 0.278 ± 0.12
0.556CysLys: 0.556 ± 0.164
0.89CysLeu: 0.89 ± 0.281
0.278CysMet: 0.278 ± 0.147
0.445CysAsn: 0.445 ± 0.162
1.391CysPro: 1.391 ± 0.329
0.278CysGln: 0.278 ± 0.148
0.723CysArg: 0.723 ± 0.224
0.668CysSer: 0.668 ± 0.207
0.89CysThr: 0.89 ± 0.219
0.668CysVal: 0.668 ± 0.178
0.278CysTrp: 0.278 ± 0.114
0.111CysTyr: 0.111 ± 0.075
0.0CysXaa: 0.0 ± 0.0
Asp
7.621AspAla: 7.621 ± 0.7
0.89AspCys: 0.89 ± 0.213
4.395AspAsp: 4.395 ± 0.449
3.894AspGlu: 3.894 ± 0.546
1.725AspPhe: 1.725 ± 0.28
6.453AspGly: 6.453 ± 0.66
1.391AspHis: 1.391 ± 0.262
2.336AspIle: 2.336 ± 0.361
1.947AspLys: 1.947 ± 0.383
6.231AspLeu: 6.231 ± 0.524
1.113AspMet: 1.113 ± 0.306
2.003AspAsn: 2.003 ± 0.412
5.285AspPro: 5.285 ± 0.6
2.058AspGln: 2.058 ± 0.338
4.784AspArg: 4.784 ± 0.605
3.171AspSer: 3.171 ± 0.479
4.283AspThr: 4.283 ± 0.447
4.117AspVal: 4.117 ± 0.493
1.335AspTrp: 1.335 ± 0.252
2.114AspTyr: 2.114 ± 0.284
0.0AspXaa: 0.0 ± 0.0
Glu
6.175GluAla: 6.175 ± 0.662
0.946GluCys: 0.946 ± 0.29
3.004GluAsp: 3.004 ± 0.373
2.726GluGlu: 2.726 ± 0.56
2.17GluPhe: 2.17 ± 0.399
3.505GluGly: 3.505 ± 0.441
1.613GluHis: 1.613 ± 0.365
2.281GluIle: 2.281 ± 0.286
2.17GluLys: 2.17 ± 0.309
5.785GluLeu: 5.785 ± 0.609
1.446GluMet: 1.446 ± 0.294
1.78GluAsn: 1.78 ± 0.271
2.67GluPro: 2.67 ± 0.475
2.837GluGln: 2.837 ± 0.388
4.784GluArg: 4.784 ± 0.575
3.06GluSer: 3.06 ± 0.45
4.84GluThr: 4.84 ± 0.635
4.117GluVal: 4.117 ± 0.529
1.669GluTrp: 1.669 ± 0.339
1.947GluTyr: 1.947 ± 0.321
0.0GluXaa: 0.0 ± 0.0
Phe
3.171PheAla: 3.171 ± 0.415
0.334PheCys: 0.334 ± 0.135
2.781PheAsp: 2.781 ± 0.521
1.502PheGlu: 1.502 ± 0.29
1.001PhePhe: 1.001 ± 0.253
3.004PheGly: 3.004 ± 0.678
0.612PheHis: 0.612 ± 0.225
1.446PheIle: 1.446 ± 0.348
1.057PheLys: 1.057 ± 0.262
1.725PheLeu: 1.725 ± 0.243
0.668PheMet: 0.668 ± 0.145
1.224PheAsn: 1.224 ± 0.306
1.446PhePro: 1.446 ± 0.288
1.224PheGln: 1.224 ± 0.319
1.502PheArg: 1.502 ± 0.259
1.78PheSer: 1.78 ± 0.284
2.392PheThr: 2.392 ± 0.425
2.003PheVal: 2.003 ± 0.318
0.668PheTrp: 0.668 ± 0.164
0.89PheTyr: 0.89 ± 0.233
0.0PheXaa: 0.0 ± 0.0
Gly
9.012GlyAla: 9.012 ± 1.051
1.168GlyCys: 1.168 ± 0.26
6.175GlyAsp: 6.175 ± 0.594
4.45GlyGlu: 4.45 ± 0.571
2.837GlyPhe: 2.837 ± 0.466
10.18GlyGly: 10.18 ± 1.937
2.114GlyHis: 2.114 ± 0.318
4.117GlyIle: 4.117 ± 0.486
2.503GlyLys: 2.503 ± 0.374
5.785GlyLeu: 5.785 ± 0.627
2.225GlyMet: 2.225 ± 0.488
2.948GlyAsn: 2.948 ± 0.323
4.061GlyPro: 4.061 ± 0.559
2.392GlyGln: 2.392 ± 0.501
5.563GlyArg: 5.563 ± 0.569
6.175GlySer: 6.175 ± 0.713
5.952GlyThr: 5.952 ± 0.805
5.73GlyVal: 5.73 ± 0.525
2.281GlyTrp: 2.281 ± 0.296
2.225GlyTyr: 2.225 ± 0.392
0.0GlyXaa: 0.0 ± 0.0
His
1.947HisAla: 1.947 ± 0.368
0.389HisCys: 0.389 ± 0.181
1.224HisAsp: 1.224 ± 0.247
1.335HisGlu: 1.335 ± 0.288
0.501HisPhe: 0.501 ± 0.137
1.891HisGly: 1.891 ± 0.359
1.113HisHis: 1.113 ± 0.306
1.279HisIle: 1.279 ± 0.289
0.946HisLys: 0.946 ± 0.227
1.502HisLeu: 1.502 ± 0.262
0.556HisMet: 0.556 ± 0.154
0.946HisAsn: 0.946 ± 0.194
1.669HisPro: 1.669 ± 0.295
0.723HisGln: 0.723 ± 0.175
1.725HisArg: 1.725 ± 0.35
0.89HisSer: 0.89 ± 0.231
1.558HisThr: 1.558 ± 0.306
1.725HisVal: 1.725 ± 0.34
0.445HisTrp: 0.445 ± 0.162
0.723HisTyr: 0.723 ± 0.16
0.0HisXaa: 0.0 ± 0.0
Ile
5.007IleAla: 5.007 ± 0.558
0.556IleCys: 0.556 ± 0.215
3.727IleAsp: 3.727 ± 0.477
3.505IleGlu: 3.505 ± 0.318
0.668IlePhe: 0.668 ± 0.221
4.228IleGly: 4.228 ± 0.492
1.446IleHis: 1.446 ± 0.328
1.558IleIle: 1.558 ± 0.273
1.057IleLys: 1.057 ± 0.236
2.336IleLeu: 2.336 ± 0.332
0.334IleMet: 0.334 ± 0.121
2.058IleAsn: 2.058 ± 0.385
2.503IlePro: 2.503 ± 0.35
1.335IleGln: 1.335 ± 0.258
2.336IleArg: 2.336 ± 0.336
2.559IleSer: 2.559 ± 0.41
3.56IleThr: 3.56 ± 0.429
2.837IleVal: 2.837 ± 0.418
1.001IleTrp: 1.001 ± 0.254
0.89IleTyr: 0.89 ± 0.206
0.0IleXaa: 0.0 ± 0.0
Lys
3.838LysAla: 3.838 ± 0.413
0.445LysCys: 0.445 ± 0.146
2.003LysAsp: 2.003 ± 0.339
1.613LysGlu: 1.613 ± 0.281
1.279LysPhe: 1.279 ± 0.221
2.559LysGly: 2.559 ± 0.322
1.335LysHis: 1.335 ± 0.326
1.224LysIle: 1.224 ± 0.291
1.446LysLys: 1.446 ± 0.415
2.336LysLeu: 2.336 ± 0.492
0.612LysMet: 0.612 ± 0.184
0.723LysAsn: 0.723 ± 0.194
2.392LysPro: 2.392 ± 0.357
1.224LysGln: 1.224 ± 0.262
2.448LysArg: 2.448 ± 0.422
2.058LysSer: 2.058 ± 0.345
2.114LysThr: 2.114 ± 0.342
2.336LysVal: 2.336 ± 0.412
1.001LysTrp: 1.001 ± 0.29
0.834LysTyr: 0.834 ± 0.199
0.0LysXaa: 0.0 ± 0.0
Leu
7.844LeuAla: 7.844 ± 0.946
0.723LeuCys: 0.723 ± 0.243
5.285LeuAsp: 5.285 ± 0.641
4.117LeuGlu: 4.117 ± 0.513
2.67LeuPhe: 2.67 ± 0.391
4.895LeuGly: 4.895 ± 0.571
0.834LeuHis: 0.834 ± 0.201
3.338LeuIle: 3.338 ± 0.486
2.058LeuLys: 2.058 ± 0.325
5.062LeuLeu: 5.062 ± 0.483
1.613LeuMet: 1.613 ± 0.276
2.726LeuAsn: 2.726 ± 0.392
5.73LeuPro: 5.73 ± 0.567
2.837LeuGln: 2.837 ± 0.422
5.229LeuArg: 5.229 ± 0.621
4.673LeuSer: 4.673 ± 0.508
5.34LeuThr: 5.34 ± 0.539
4.895LeuVal: 4.895 ± 0.463
1.279LeuTrp: 1.279 ± 0.294
2.114LeuTyr: 2.114 ± 0.423
0.0LeuXaa: 0.0 ± 0.0
Met
2.281MetAla: 2.281 ± 0.324
0.167MetCys: 0.167 ± 0.132
1.502MetAsp: 1.502 ± 0.249
0.89MetGlu: 0.89 ± 0.186
0.668MetPhe: 0.668 ± 0.229
1.891MetGly: 1.891 ± 0.275
0.223MetHis: 0.223 ± 0.119
1.113MetIle: 1.113 ± 0.213
0.723MetLys: 0.723 ± 0.222
1.613MetLeu: 1.613 ± 0.295
0.389MetMet: 0.389 ± 0.218
1.001MetAsn: 1.001 ± 0.221
1.558MetPro: 1.558 ± 0.286
0.668MetGln: 0.668 ± 0.189
1.558MetArg: 1.558 ± 0.296
2.781MetSer: 2.781 ± 0.398
2.114MetThr: 2.114 ± 0.286
1.335MetVal: 1.335 ± 0.308
0.223MetTrp: 0.223 ± 0.106
0.223MetTyr: 0.223 ± 0.114
0.0MetXaa: 0.0 ± 0.0
Asn
3.727AsnAla: 3.727 ± 0.477
0.278AsnCys: 0.278 ± 0.131
2.114AsnAsp: 2.114 ± 0.29
1.613AsnGlu: 1.613 ± 0.331
0.723AsnPhe: 0.723 ± 0.277
3.95AsnGly: 3.95 ± 0.597
0.946AsnHis: 0.946 ± 0.171
1.446AsnIle: 1.446 ± 0.405
1.168AsnLys: 1.168 ± 0.259
2.336AsnLeu: 2.336 ± 0.368
0.668AsnMet: 0.668 ± 0.169
1.947AsnAsn: 1.947 ± 0.333
2.781AsnPro: 2.781 ± 0.336
1.224AsnGln: 1.224 ± 0.367
1.891AsnArg: 1.891 ± 0.367
1.446AsnSer: 1.446 ± 0.297
2.225AsnThr: 2.225 ± 0.281
1.836AsnVal: 1.836 ± 0.301
0.834AsnTrp: 0.834 ± 0.199
0.612AsnTyr: 0.612 ± 0.187
0.0AsnXaa: 0.0 ± 0.0
Pro
5.396ProAla: 5.396 ± 0.531
0.723ProCys: 0.723 ± 0.203
4.729ProAsp: 4.729 ± 0.565
4.506ProGlu: 4.506 ± 0.417
2.114ProPhe: 2.114 ± 0.32
6.342ProGly: 6.342 ± 0.715
1.558ProHis: 1.558 ± 0.301
1.947ProIle: 1.947 ± 0.307
2.281ProLys: 2.281 ± 0.349
4.395ProLeu: 4.395 ± 0.555
1.613ProMet: 1.613 ± 0.354
1.947ProAsn: 1.947 ± 0.306
3.894ProPro: 3.894 ± 0.557
2.281ProGln: 2.281 ± 0.368
3.115ProArg: 3.115 ± 0.588
3.393ProSer: 3.393 ± 0.45
3.282ProThr: 3.282 ± 0.433
4.895ProVal: 4.895 ± 0.637
1.224ProTrp: 1.224 ± 0.243
1.502ProTyr: 1.502 ± 0.276
0.0ProXaa: 0.0 ± 0.0
Gln
4.172GlnAla: 4.172 ± 0.663
0.389GlnCys: 0.389 ± 0.151
1.391GlnAsp: 1.391 ± 0.246
1.613GlnGlu: 1.613 ± 0.295
1.001GlnPhe: 1.001 ± 0.234
2.559GlnGly: 2.559 ± 0.396
0.946GlnHis: 0.946 ± 0.251
1.558GlnIle: 1.558 ± 0.286
1.335GlnLys: 1.335 ± 0.269
3.115GlnLeu: 3.115 ± 0.36
0.779GlnMet: 0.779 ± 0.193
0.946GlnAsn: 0.946 ± 0.226
2.781GlnPro: 2.781 ± 0.432
1.001GlnGln: 1.001 ± 0.21
2.615GlnArg: 2.615 ± 0.394
2.281GlnSer: 2.281 ± 0.366
1.891GlnThr: 1.891 ± 0.302
2.114GlnVal: 2.114 ± 0.308
0.556GlnTrp: 0.556 ± 0.142
0.779GlnTyr: 0.779 ± 0.263
0.0GlnXaa: 0.0 ± 0.0
Arg
6.453ArgAla: 6.453 ± 0.692
1.168ArgCys: 1.168 ± 0.302
4.784ArgAsp: 4.784 ± 0.47
4.562ArgGlu: 4.562 ± 0.604
2.225ArgPhe: 2.225 ± 0.446
4.172ArgGly: 4.172 ± 0.429
1.279ArgHis: 1.279 ± 0.346
4.228ArgIle: 4.228 ± 0.557
2.336ArgLys: 2.336 ± 0.336
4.951ArgLeu: 4.951 ± 0.612
2.837ArgMet: 2.837 ± 0.403
2.448ArgAsn: 2.448 ± 0.391
3.783ArgPro: 3.783 ± 0.525
1.891ArgGln: 1.891 ± 0.417
5.507ArgArg: 5.507 ± 0.837
3.616ArgSer: 3.616 ± 0.502
3.338ArgThr: 3.338 ± 0.41
4.729ArgVal: 4.729 ± 0.493
2.003ArgTrp: 2.003 ± 0.353
1.947ArgTyr: 1.947 ± 0.317
0.0ArgXaa: 0.0 ± 0.0
Ser
5.229SerAla: 5.229 ± 0.742
0.612SerCys: 0.612 ± 0.257
3.727SerAsp: 3.727 ± 0.526
3.171SerGlu: 3.171 ± 0.471
2.17SerPhe: 2.17 ± 0.49
5.952SerGly: 5.952 ± 0.885
0.946SerHis: 0.946 ± 0.246
3.004SerIle: 3.004 ± 0.468
2.503SerLys: 2.503 ± 0.4
3.838SerLeu: 3.838 ± 0.429
1.502SerMet: 1.502 ± 0.284
2.058SerAsn: 2.058 ± 0.428
3.282SerPro: 3.282 ± 0.418
1.836SerGln: 1.836 ± 0.315
3.56SerArg: 3.56 ± 0.516
3.894SerSer: 3.894 ± 0.557
3.449SerThr: 3.449 ± 0.444
4.339SerVal: 4.339 ± 0.532
1.279SerTrp: 1.279 ± 0.308
1.279SerTyr: 1.279 ± 0.224
0.0SerXaa: 0.0 ± 0.0
Thr
6.731ThrAla: 6.731 ± 0.607
0.779ThrCys: 0.779 ± 0.231
3.393ThrAsp: 3.393 ± 0.519
4.283ThrGlu: 4.283 ± 0.51
2.114ThrPhe: 2.114 ± 0.406
6.286ThrGly: 6.286 ± 0.654
1.725ThrHis: 1.725 ± 0.284
3.505ThrIle: 3.505 ± 0.466
2.003ThrLys: 2.003 ± 0.284
4.339ThrLeu: 4.339 ± 0.548
1.335ThrMet: 1.335 ± 0.277
2.448ThrAsn: 2.448 ± 0.374
4.339ThrPro: 4.339 ± 0.525
1.947ThrGln: 1.947 ± 0.38
4.395ThrArg: 4.395 ± 0.47
3.672ThrSer: 3.672 ± 0.45
5.007ThrThr: 5.007 ± 0.67
5.619ThrVal: 5.619 ± 0.571
1.113ThrTrp: 1.113 ± 0.253
1.891ThrTyr: 1.891 ± 0.303
0.0ThrXaa: 0.0 ± 0.0
Val
7.51ValAla: 7.51 ± 0.651
1.446ValCys: 1.446 ± 0.315
5.229ValAsp: 5.229 ± 0.545
4.395ValGlu: 4.395 ± 0.578
2.281ValPhe: 2.281 ± 0.398
5.507ValGly: 5.507 ± 0.742
1.391ValHis: 1.391 ± 0.256
2.448ValIle: 2.448 ± 0.35
2.503ValLys: 2.503 ± 0.319
5.007ValLeu: 5.007 ± 0.516
1.446ValMet: 1.446 ± 0.27
1.947ValAsn: 1.947 ± 0.325
3.95ValPro: 3.95 ± 0.429
2.726ValGln: 2.726 ± 0.386
4.784ValArg: 4.784 ± 0.651
4.061ValSer: 4.061 ± 0.538
4.562ValThr: 4.562 ± 0.433
5.952ValVal: 5.952 ± 0.615
1.947ValTrp: 1.947 ± 0.366
1.391ValTyr: 1.391 ± 0.265
0.0ValXaa: 0.0 ± 0.0
Trp
2.058TrpAla: 2.058 ± 0.289
0.389TrpCys: 0.389 ± 0.173
1.224TrpAsp: 1.224 ± 0.293
1.168TrpGlu: 1.168 ± 0.331
0.779TrpPhe: 0.779 ± 0.173
0.946TrpGly: 0.946 ± 0.23
0.723TrpHis: 0.723 ± 0.226
1.001TrpIle: 1.001 ± 0.212
0.668TrpLys: 0.668 ± 0.136
1.836TrpLeu: 1.836 ± 0.32
0.89TrpMet: 0.89 ± 0.231
0.612TrpAsn: 0.612 ± 0.186
0.946TrpPro: 0.946 ± 0.291
1.057TrpGln: 1.057 ± 0.244
2.503TrpArg: 2.503 ± 0.414
1.224TrpSer: 1.224 ± 0.21
1.725TrpThr: 1.725 ± 0.319
2.114TrpVal: 2.114 ± 0.476
0.89TrpTrp: 0.89 ± 0.22
0.334TrpTyr: 0.334 ± 0.145
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.615TyrAla: 2.615 ± 0.322
0.389TyrCys: 0.389 ± 0.172
1.891TyrAsp: 1.891 ± 0.404
1.78TyrGlu: 1.78 ± 0.321
0.612TyrPhe: 0.612 ± 0.18
1.891TyrGly: 1.891 ± 0.343
0.167TyrHis: 0.167 ± 0.074
1.001TyrIle: 1.001 ± 0.198
0.723TyrLys: 0.723 ± 0.191
2.225TyrLeu: 2.225 ± 0.335
0.167TyrMet: 0.167 ± 0.092
0.834TyrAsn: 0.834 ± 0.21
1.335TyrPro: 1.335 ± 0.259
0.779TyrGln: 0.779 ± 0.174
2.281TyrArg: 2.281 ± 0.354
0.779TyrSer: 0.779 ± 0.215
1.78TyrThr: 1.78 ± 0.349
2.225TyrVal: 2.225 ± 0.357
0.612TyrTrp: 0.612 ± 0.191
0.89TyrTyr: 0.89 ± 0.191
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 99 proteins (17977 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski