Squash leaf curl China virus - [B]

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus; Squash leaf curl China virus

Average proteome isoelectric point is 8.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q7T488|Q7T488_9GEMI Capsid protein OS=Squash leaf curl China virus - [B] OX=269275 GN=AV1 PE=3 SV=1
MM1 pKa = 7.79APPKK5 pKa = 10.13HH6 pKa = 6.04FKK8 pKa = 10.35ISAKK12 pKa = 10.47NYY14 pKa = 7.93FLTYY18 pKa = 9.1PKK20 pKa = 10.43CSLSKK25 pKa = 11.11EE26 pKa = 4.14EE27 pKa = 5.07ALSQLRR33 pKa = 11.84TLEE36 pKa = 4.22TPTSKK41 pKa = 10.73KK42 pKa = 9.91FIKK45 pKa = 9.76ICRR48 pKa = 11.84EE49 pKa = 3.79LHH51 pKa = 6.29EE52 pKa = 6.05DD53 pKa = 3.97GAPHH57 pKa = 6.07IHH59 pKa = 6.5VLIQFEE65 pKa = 5.43GKK67 pKa = 9.21FQCKK71 pKa = 8.51NNRR74 pKa = 11.84FFDD77 pKa = 3.93LVSPSRR83 pKa = 11.84STHH86 pKa = 4.86FHH88 pKa = 6.77PNIQGAKK95 pKa = 8.78SASDD99 pKa = 3.14VKK101 pKa = 11.06QYY103 pKa = 10.61IDD105 pKa = 3.46KK106 pKa = 11.28DD107 pKa = 3.77GDD109 pKa = 3.66VLEE112 pKa = 4.69WGVFQIDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQTANDD132 pKa = 3.71AYY134 pKa = 10.81ARR136 pKa = 11.84AINTGNKK143 pKa = 9.11EE144 pKa = 3.96DD145 pKa = 3.66ALKK148 pKa = 10.71VLKK151 pKa = 10.33EE152 pKa = 4.25LAPKK156 pKa = 10.39DD157 pKa = 3.72YY158 pKa = 11.21VLQFHH163 pKa = 6.57NMNSNLDD170 pKa = 3.89RR171 pKa = 11.84IFPPRR176 pKa = 11.84AEE178 pKa = 4.37VYY180 pKa = 10.26VSPFSISSFDD190 pKa = 3.45RR191 pKa = 11.84VPQEE195 pKa = 3.6LVDD198 pKa = 3.73WVSSNVGCCAARR210 pKa = 11.84PNRR213 pKa = 11.84PIGIVLEE220 pKa = 4.47GDD222 pKa = 3.28SRR224 pKa = 11.84TGKK227 pKa = 7.46TAWARR232 pKa = 11.84SLGPHH237 pKa = 6.71NYY239 pKa = 10.18LCGHH243 pKa = 7.38LDD245 pKa = 3.88LSPRR249 pKa = 11.84VYY251 pKa = 11.34NNDD254 pKa = 2.06AWYY257 pKa = 10.88NVIDD261 pKa = 5.2DD262 pKa = 4.41VDD264 pKa = 3.61PHH266 pKa = 5.85YY267 pKa = 11.0LKK269 pKa = 10.7HH270 pKa = 6.2FKK272 pKa = 10.7EE273 pKa = 4.46FMGAQRR279 pKa = 11.84DD280 pKa = 3.81WQSNTKK286 pKa = 9.47YY287 pKa = 10.28GKK289 pKa = 9.35PVMIKK294 pKa = 10.55GGIPTIFLCNKK305 pKa = 9.29GPNSSYY311 pKa = 11.07KK312 pKa = 10.45EE313 pKa = 3.93YY314 pKa = 11.01LDD316 pKa = 3.84EE317 pKa = 4.8EE318 pKa = 4.44KK319 pKa = 10.99NAALKK324 pKa = 10.02QWALKK329 pKa = 9.99NAIFITIEE337 pKa = 3.72EE338 pKa = 4.2PLYY341 pKa = 10.79SGRR344 pKa = 11.84EE345 pKa = 4.07SIATAEE351 pKa = 4.31EE352 pKa = 4.42EE353 pKa = 4.81EE354 pKa = 4.38EE355 pKa = 4.34HH356 pKa = 5.09QTT358 pKa = 4.3

Molecular weight: 40.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q7T489|Q7T489_9GEMI Movement protein BC1 OS=Squash leaf curl China virus - [B] OX=269275 GN=BC1 PE=3 SV=1
MM1 pKa = 7.71SKK3 pKa = 10.35RR4 pKa = 11.84PADD7 pKa = 3.87IIISTPASKK16 pKa = 10.3VRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84LNFDD24 pKa = 3.04SPYY27 pKa = 9.72VSRR30 pKa = 11.84AVVPIARR37 pKa = 11.84VTKK40 pKa = 10.21GKK42 pKa = 10.45AWTNRR47 pKa = 11.84PMNRR51 pKa = 11.84KK52 pKa = 7.81PKK54 pKa = 8.24MYY56 pKa = 10.47RR57 pKa = 11.84MYY59 pKa = 10.58RR60 pKa = 11.84SPDD63 pKa = 3.21VPRR66 pKa = 11.84GCEE69 pKa = 4.34GPCKK73 pKa = 10.01VQSFEE78 pKa = 4.06SRR80 pKa = 11.84HH81 pKa = 5.57DD82 pKa = 3.47VSHH85 pKa = 6.93IGKK88 pKa = 9.37VMCISDD94 pKa = 3.67VTRR97 pKa = 11.84GTGLTHH103 pKa = 7.09RR104 pKa = 11.84VGKK107 pKa = 9.66RR108 pKa = 11.84FCVKK112 pKa = 9.94SVYY115 pKa = 10.52VLGKK119 pKa = 9.41IWMDD123 pKa = 3.35EE124 pKa = 4.04NIKK127 pKa = 9.32TKK129 pKa = 10.56NHH131 pKa = 5.33TNSVIFFLVQDD142 pKa = 4.22RR143 pKa = 11.84RR144 pKa = 11.84PTGTPQDD151 pKa = 3.67FGEE154 pKa = 4.38VFNMFDD160 pKa = 4.29NEE162 pKa = 4.11PSTATVKK169 pKa = 10.61NMHH172 pKa = 6.98RR173 pKa = 11.84DD174 pKa = 3.26RR175 pKa = 11.84YY176 pKa = 9.02QVMRR180 pKa = 11.84KK181 pKa = 6.32WHH183 pKa = 5.44ATVTGGTYY191 pKa = 10.6ASRR194 pKa = 11.84EE195 pKa = 3.76QALVRR200 pKa = 11.84KK201 pKa = 8.27FVRR204 pKa = 11.84VNNYY208 pKa = 7.82VVYY211 pKa = 9.96NQQEE215 pKa = 3.8AGKK218 pKa = 10.1YY219 pKa = 8.23EE220 pKa = 4.02NHH222 pKa = 6.47TEE224 pKa = 3.99NALMLYY230 pKa = 7.52MACTHH235 pKa = 7.06ASNPVYY241 pKa = 9.86ATLKK245 pKa = 9.47IRR247 pKa = 11.84IYY249 pKa = 10.64FYY251 pKa = 11.34DD252 pKa = 3.32SVTNN256 pKa = 3.98

Molecular weight: 29.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

1766

134

358

220.8

25.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.21 ± 0.49

2.322 ± 0.239

4.643 ± 0.393

4.7 ± 0.662

3.964 ± 0.287

5.606 ± 0.179

3.737 ± 0.515

6.059 ± 0.609

6.059 ± 0.482

6.965 ± 0.692

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.492 ± 0.43

5.663 ± 0.293

5.21 ± 0.379

3.398 ± 0.358

6.852 ± 0.663

7.928 ± 1.05

6.285 ± 0.716

7.305 ± 0.859

1.416 ± 0.12

4.19 ± 0.556
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski