Amino acid dipepetide frequency for Bombilactobacillus mellifer

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.534AlaAla: 8.534 ± 0.176
0.556AlaCys: 0.556 ± 0.036
4.463AlaAsp: 4.463 ± 0.1
3.571AlaGlu: 3.571 ± 0.096
2.972AlaPhe: 2.972 ± 0.084
5.693AlaGly: 5.693 ± 0.12
1.99AlaHis: 1.99 ± 0.059
5.654AlaIle: 5.654 ± 0.121
4.123AlaLys: 4.123 ± 0.114
8.157AlaLeu: 8.157 ± 0.155
1.853AlaMet: 1.853 ± 0.077
4.038AlaAsn: 4.038 ± 0.117
2.783AlaPro: 2.783 ± 0.07
6.908AlaGln: 6.908 ± 0.153
2.983AlaArg: 2.983 ± 0.082
4.088AlaSer: 4.088 ± 0.092
5.175AlaThr: 5.175 ± 0.124
5.848AlaVal: 5.848 ± 0.124
0.741AlaTrp: 0.741 ± 0.035
2.458AlaTyr: 2.458 ± 0.077
0.0AlaXaa: 0.0 ± 0.0
Cys
0.475CysAla: 0.475 ± 0.029
0.08CysCys: 0.08 ± 0.013
0.315CysAsp: 0.315 ± 0.022
0.239CysGlu: 0.239 ± 0.022
0.266CysPhe: 0.266 ± 0.023
0.655CysGly: 0.655 ± 0.032
0.22CysHis: 0.22 ± 0.022
0.381CysIle: 0.381 ± 0.028
0.218CysLys: 0.218 ± 0.023
0.778CysLeu: 0.778 ± 0.043
0.091CysMet: 0.091 ± 0.014
0.294CysAsn: 0.294 ± 0.027
0.313CysPro: 0.313 ± 0.029
0.467CysGln: 0.467 ± 0.037
0.3CysArg: 0.3 ± 0.025
0.453CysSer: 0.453 ± 0.038
0.35CysThr: 0.35 ± 0.027
0.325CysVal: 0.325 ± 0.025
0.068CysTrp: 0.068 ± 0.011
0.255CysTyr: 0.255 ± 0.022
0.0CysXaa: 0.0 ± 0.0
Asp
3.485AspAla: 3.485 ± 0.088
0.37AspCys: 0.37 ± 0.028
2.53AspAsp: 2.53 ± 0.083
2.548AspGlu: 2.548 ± 0.071
2.651AspPhe: 2.651 ± 0.07
2.639AspGly: 2.639 ± 0.082
1.406AspHis: 1.406 ± 0.061
3.219AspIle: 3.219 ± 0.087
2.486AspLys: 2.486 ± 0.085
5.978AspLeu: 5.978 ± 0.119
1.194AspMet: 1.194 ± 0.061
2.351AspAsn: 2.351 ± 0.071
2.559AspPro: 2.559 ± 0.075
3.722AspGln: 3.722 ± 0.087
1.918AspArg: 1.918 ± 0.061
2.865AspSer: 2.865 ± 0.084
2.606AspThr: 2.606 ± 0.087
3.215AspVal: 3.215 ± 0.092
0.665AspTrp: 0.665 ± 0.037
2.431AspTyr: 2.431 ± 0.068
0.0AspXaa: 0.0 ± 0.0
Glu
3.627GluAla: 3.627 ± 0.099
0.231GluCys: 0.231 ± 0.023
2.062GluAsp: 2.062 ± 0.067
2.223GluGlu: 2.223 ± 0.081
1.811GluPhe: 1.811 ± 0.064
1.925GluGly: 1.925 ± 0.071
1.204GluHis: 1.204 ± 0.051
3.617GluIle: 3.617 ± 0.081
3.016GluLys: 3.016 ± 0.089
4.835GluLeu: 4.835 ± 0.102
1.299GluMet: 1.299 ± 0.049
2.133GluAsn: 2.133 ± 0.071
1.589GluPro: 1.589 ± 0.059
3.23GluGln: 3.23 ± 0.095
1.923GluArg: 1.923 ± 0.066
1.968GluSer: 1.968 ± 0.065
2.458GluThr: 2.458 ± 0.07
3.248GluVal: 3.248 ± 0.076
0.348GluTrp: 0.348 ± 0.028
1.505GluTyr: 1.505 ± 0.058
0.0GluXaa: 0.0 ± 0.0
Phe
3.217PheAla: 3.217 ± 0.078
0.335PheCys: 0.335 ± 0.026
2.487PheAsp: 2.487 ± 0.066
1.754PheGlu: 1.754 ± 0.061
1.923PhePhe: 1.923 ± 0.08
2.756PheGly: 2.756 ± 0.091
0.908PheHis: 0.908 ± 0.041
2.939PheIle: 2.939 ± 0.082
2.087PheLys: 2.087 ± 0.071
4.335PheLeu: 4.335 ± 0.116
0.92PheMet: 0.92 ± 0.049
2.338PheAsn: 2.338 ± 0.083
1.464PhePro: 1.464 ± 0.05
2.33PheGln: 2.33 ± 0.065
1.264PheArg: 1.264 ± 0.05
2.612PheSer: 2.612 ± 0.082
2.281PheThr: 2.281 ± 0.069
2.766PheVal: 2.766 ± 0.084
0.599PheTrp: 0.599 ± 0.035
1.603PheTyr: 1.603 ± 0.056
0.0PheXaa: 0.0 ± 0.0
Gly
4.605GlyAla: 4.605 ± 0.104
0.562GlyCys: 0.562 ± 0.041
2.89GlyAsp: 2.89 ± 0.097
2.604GlyGlu: 2.604 ± 0.077
2.791GlyPhe: 2.791 ± 0.077
3.927GlyGly: 3.927 ± 0.119
1.591GlyHis: 1.591 ± 0.051
4.905GlyIle: 4.905 ± 0.108
3.275GlyLys: 3.275 ± 0.077
6.542GlyLeu: 6.542 ± 0.141
1.634GlyMet: 1.634 ± 0.06
2.608GlyAsn: 2.608 ± 0.075
1.77GlyPro: 1.77 ± 0.063
3.602GlyGln: 3.602 ± 0.095
2.427GlyArg: 2.427 ± 0.069
3.925GlySer: 3.925 ± 0.093
4.02GlyThr: 4.02 ± 0.11
4.218GlyVal: 4.218 ± 0.084
0.661GlyTrp: 0.661 ± 0.036
2.507GlyTyr: 2.507 ± 0.082
0.0GlyXaa: 0.0 ± 0.0
His
1.501HisAla: 1.501 ± 0.051
0.216HisCys: 0.216 ± 0.022
1.216HisAsp: 1.216 ± 0.05
1.074HisGlu: 1.074 ± 0.047
1.251HisPhe: 1.251 ± 0.048
1.585HisGly: 1.585 ± 0.059
0.807HisHis: 0.807 ± 0.044
1.404HisIle: 1.404 ± 0.047
1.13HisLys: 1.13 ± 0.051
2.962HisLeu: 2.962 ± 0.077
0.502HisMet: 0.502 ± 0.031
1.134HisAsn: 1.134 ± 0.047
1.221HisPro: 1.221 ± 0.05
1.877HisGln: 1.877 ± 0.054
0.972HisArg: 0.972 ± 0.052
1.291HisSer: 1.291 ± 0.053
1.151HisThr: 1.151 ± 0.053
1.385HisVal: 1.385 ± 0.053
0.406HisTrp: 0.406 ± 0.032
1.165HisTyr: 1.165 ± 0.05
0.0HisXaa: 0.0 ± 0.0
Ile
6.214IleAla: 6.214 ± 0.124
0.593IleCys: 0.593 ± 0.031
3.932IleAsp: 3.932 ± 0.094
3.067IleGlu: 3.067 ± 0.09
3.092IlePhe: 3.092 ± 0.092
4.8IleGly: 4.8 ± 0.112
1.418IleHis: 1.418 ± 0.057
5.597IleIle: 5.597 ± 0.129
4.255IleLys: 4.255 ± 0.104
7.011IleLeu: 7.011 ± 0.136
1.665IleMet: 1.665 ± 0.064
3.966IleAsn: 3.966 ± 0.105
2.902IlePro: 2.902 ± 0.064
3.822IleGln: 3.822 ± 0.09
2.384IleArg: 2.384 ± 0.074
4.36IleSer: 4.36 ± 0.094
4.561IleThr: 4.561 ± 0.092
5.142IleVal: 5.142 ± 0.112
0.679IleTrp: 0.679 ± 0.036
2.439IleTyr: 2.439 ± 0.072
0.0IleXaa: 0.0 ± 0.0
Lys
3.997LysAla: 3.997 ± 0.091
0.171LysCys: 0.171 ± 0.017
2.76LysAsp: 2.76 ± 0.093
2.449LysGlu: 2.449 ± 0.079
1.993LysPhe: 1.993 ± 0.066
2.528LysGly: 2.528 ± 0.066
1.186LysHis: 1.186 ± 0.052
4.738LysIle: 4.738 ± 0.113
4.286LysLys: 4.286 ± 0.107
5.704LysLeu: 5.704 ± 0.112
1.69LysMet: 1.69 ± 0.055
3.287LysAsn: 3.287 ± 0.099
1.997LysPro: 1.997 ± 0.069
3.621LysGln: 3.621 ± 0.102
2.287LysArg: 2.287 ± 0.068
3.065LysSer: 3.065 ± 0.087
3.732LysThr: 3.732 ± 0.092
3.816LysVal: 3.816 ± 0.081
0.496LysTrp: 0.496 ± 0.032
2.357LysTyr: 2.357 ± 0.072
0.0LysXaa: 0.0 ± 0.0
Leu
9.304LeuAla: 9.304 ± 0.141
0.681LeuCys: 0.681 ± 0.038
5.307LeuAsp: 5.307 ± 0.114
4.395LeuGlu: 4.395 ± 0.095
3.814LeuPhe: 3.814 ± 0.106
6.603LeuGly: 6.603 ± 0.129
2.289LeuHis: 2.289 ± 0.074
7.717LeuIle: 7.717 ± 0.144
6.097LeuLys: 6.097 ± 0.114
10.271LeuLeu: 10.271 ± 0.22
2.517LeuMet: 2.517 ± 0.073
5.269LeuAsn: 5.269 ± 0.121
4.72LeuPro: 4.72 ± 0.1
7.194LeuGln: 7.194 ± 0.155
3.822LeuArg: 3.822 ± 0.098
6.554LeuSer: 6.554 ± 0.134
7.404LeuThr: 7.404 ± 0.133
7.042LeuVal: 7.042 ± 0.137
0.982LeuTrp: 0.982 ± 0.053
2.981LeuTyr: 2.981 ± 0.086
0.0LeuXaa: 0.0 ± 0.0
Met
2.091MetAla: 2.091 ± 0.061
0.148MetCys: 0.148 ± 0.014
1.233MetAsp: 1.233 ± 0.047
1.035MetGlu: 1.035 ± 0.052
1.126MetPhe: 1.126 ± 0.095
1.536MetGly: 1.536 ± 0.053
0.475MetHis: 0.475 ± 0.031
1.713MetIle: 1.713 ± 0.053
1.591MetLys: 1.591 ± 0.047
2.211MetLeu: 2.211 ± 0.062
0.615MetMet: 0.615 ± 0.035
1.223MetAsn: 1.223 ± 0.048
0.926MetPro: 0.926 ± 0.044
1.305MetGln: 1.305 ± 0.056
0.891MetArg: 0.891 ± 0.044
1.568MetSer: 1.568 ± 0.065
1.603MetThr: 1.603 ± 0.055
1.529MetVal: 1.529 ± 0.062
0.15MetTrp: 0.15 ± 0.015
0.543MetTyr: 0.543 ± 0.034
0.0MetXaa: 0.0 ± 0.0
Asn
3.39AsnAla: 3.39 ± 0.087
0.358AsnCys: 0.358 ± 0.028
2.526AsnAsp: 2.526 ± 0.081
2.087AsnGlu: 2.087 ± 0.072
2.363AsnPhe: 2.363 ± 0.069
2.853AsnGly: 2.853 ± 0.07
1.377AsnHis: 1.377 ± 0.055
3.398AsnIle: 3.398 ± 0.094
2.696AsnLys: 2.696 ± 0.095
5.551AsnLeu: 5.551 ± 0.103
1.182AsnMet: 1.182 ± 0.056
3.223AsnAsn: 3.223 ± 0.138
2.486AsnPro: 2.486 ± 0.074
3.503AsnGln: 3.503 ± 0.113
1.758AsnArg: 1.758 ± 0.071
2.952AsnSer: 2.952 ± 0.101
2.686AsnThr: 2.686 ± 0.112
3.114AsnVal: 3.114 ± 0.097
0.692AsnTrp: 0.692 ± 0.033
2.299AsnTyr: 2.299 ± 0.062
0.0AsnXaa: 0.0 ± 0.0
Pro
3.45ProAla: 3.45 ± 0.094
0.167ProCys: 0.167 ± 0.016
2.2ProAsp: 2.2 ± 0.072
2.377ProGlu: 2.377 ± 0.069
1.453ProPhe: 1.453 ± 0.056
2.274ProGly: 2.274 ± 0.071
1.05ProHis: 1.05 ± 0.045
2.678ProIle: 2.678 ± 0.069
2.021ProLys: 2.021 ± 0.061
4.061ProLeu: 4.061 ± 0.093
0.677ProMet: 0.677 ± 0.031
1.978ProAsn: 1.978 ± 0.062
0.974ProPro: 0.974 ± 0.042
3.575ProGln: 3.575 ± 0.091
1.381ProArg: 1.381 ± 0.061
2.083ProSer: 2.083 ± 0.062
2.721ProThr: 2.721 ± 0.083
2.933ProVal: 2.933 ± 0.079
0.389ProTrp: 0.389 ± 0.031
1.367ProTyr: 1.367 ± 0.047
0.0ProXaa: 0.0 ± 0.0
Gln
6.743GlnAla: 6.743 ± 0.14
0.253GlnCys: 0.253 ± 0.022
2.768GlnAsp: 2.768 ± 0.08
3.188GlnGlu: 3.188 ± 0.085
2.132GlnPhe: 2.132 ± 0.071
3.227GlnGly: 3.227 ± 0.079
1.737GlnHis: 1.737 ± 0.062
5.271GlnIle: 5.271 ± 0.116
4.843GlnLys: 4.843 ± 0.109
7.781GlnLeu: 7.781 ± 0.185
1.583GlnMet: 1.583 ± 0.062
3.102GlnAsn: 3.102 ± 0.082
2.935GlnPro: 2.935 ± 0.087
6.714GlnGln: 6.714 ± 0.219
3.355GlnArg: 3.355 ± 0.091
3.17GlnSer: 3.17 ± 0.093
4.732GlnThr: 4.732 ± 0.112
4.843GlnVal: 4.843 ± 0.108
0.64GlnTrp: 0.64 ± 0.037
2.036GlnTyr: 2.036 ± 0.069
0.0GlnXaa: 0.0 ± 0.0
Arg
2.686ArgAla: 2.686 ± 0.08
0.183ArgCys: 0.183 ± 0.02
1.774ArgAsp: 1.774 ± 0.058
1.756ArgGlu: 1.756 ± 0.064
1.604ArgPhe: 1.604 ± 0.054
2.18ArgGly: 2.18 ± 0.075
1.116ArgHis: 1.116 ± 0.047
2.561ArgIle: 2.561 ± 0.075
2.122ArgLys: 2.122 ± 0.063
4.069ArgLeu: 4.069 ± 0.094
0.916ArgMet: 0.916 ± 0.044
1.748ArgAsn: 1.748 ± 0.068
1.56ArgPro: 1.56 ± 0.056
3.267ArgGln: 3.267 ± 0.094
1.986ArgArg: 1.986 ± 0.081
2.077ArgSer: 2.077 ± 0.071
2.155ArgThr: 2.155 ± 0.068
2.511ArgVal: 2.511 ± 0.074
0.426ArgTrp: 0.426 ± 0.027
1.507ArgTyr: 1.507 ± 0.057
0.0ArgXaa: 0.0 ± 0.0
Ser
4.438SerAla: 4.438 ± 0.103
0.373SerCys: 0.373 ± 0.027
2.952SerAsp: 2.952 ± 0.074
2.563SerGlu: 2.563 ± 0.074
2.447SerPhe: 2.447 ± 0.079
4.115SerGly: 4.115 ± 0.112
1.375SerHis: 1.375 ± 0.057
3.635SerIle: 3.635 ± 0.095
3.219SerLys: 3.219 ± 0.085
6.136SerLeu: 6.136 ± 0.122
1.422SerMet: 1.422 ± 0.046
2.941SerAsn: 2.941 ± 0.104
2.147SerPro: 2.147 ± 0.071
3.754SerGln: 3.754 ± 0.095
2.122SerArg: 2.122 ± 0.062
3.691SerSer: 3.691 ± 0.109
3.392SerThr: 3.392 ± 0.09
3.549SerVal: 3.549 ± 0.088
0.712SerTrp: 0.712 ± 0.036
2.135SerTyr: 2.135 ± 0.064
0.0SerXaa: 0.0 ± 0.0
Thr
5.87ThrAla: 5.87 ± 0.126
0.309ThrCys: 0.309 ± 0.025
3.298ThrAsp: 3.298 ± 0.084
2.462ThrGlu: 2.462 ± 0.064
2.423ThrPhe: 2.423 ± 0.069
4.216ThrGly: 4.216 ± 0.096
1.412ThrHis: 1.412 ± 0.051
4.563ThrIle: 4.563 ± 0.104
3.456ThrLys: 3.456 ± 0.086
6.109ThrLeu: 6.109 ± 0.119
1.214ThrMet: 1.214 ± 0.04
3.427ThrAsn: 3.427 ± 0.129
2.843ThrPro: 2.843 ± 0.091
3.831ThrGln: 3.831 ± 0.112
1.898ThrArg: 1.898 ± 0.064
3.878ThrSer: 3.878 ± 0.119
4.658ThrThr: 4.658 ± 0.132
4.376ThrVal: 4.376 ± 0.095
0.714ThrTrp: 0.714 ± 0.04
1.865ThrTyr: 1.865 ± 0.059
0.0ThrXaa: 0.0 ± 0.0
Val
6.045ValAla: 6.045 ± 0.12
0.583ValCys: 0.583 ± 0.037
3.693ValAsp: 3.693 ± 0.088
2.935ValGlu: 2.935 ± 0.079
2.552ValPhe: 2.552 ± 0.08
4.617ValGly: 4.617 ± 0.122
1.338ValHis: 1.338 ± 0.056
5.243ValIle: 5.243 ± 0.103
3.524ValLys: 3.524 ± 0.084
6.69ValLeu: 6.69 ± 0.12
1.583ValMet: 1.583 ± 0.06
3.357ValAsn: 3.357 ± 0.089
2.758ValPro: 2.758 ± 0.075
4.139ValGln: 4.139 ± 0.096
2.299ValArg: 2.299 ± 0.077
3.886ValSer: 3.886 ± 0.089
4.625ValThr: 4.625 ± 0.113
4.934ValVal: 4.934 ± 0.112
0.618ValTrp: 0.618 ± 0.031
2.067ValTyr: 2.067 ± 0.067
0.0ValXaa: 0.0 ± 0.0
Trp
0.611TrpAla: 0.611 ± 0.035
0.072TrpCys: 0.072 ± 0.012
0.511TrpAsp: 0.511 ± 0.035
0.438TrpGlu: 0.438 ± 0.03
0.463TrpPhe: 0.463 ± 0.034
0.735TrpGly: 0.735 ± 0.038
0.305TrpHis: 0.305 ± 0.026
0.667TrpIle: 0.667 ± 0.039
0.408TrpLys: 0.408 ± 0.028
1.383TrpLeu: 1.383 ± 0.055
0.263TrpMet: 0.263 ± 0.028
0.449TrpAsn: 0.449 ± 0.035
0.418TrpPro: 0.418 ± 0.031
0.996TrpGln: 0.996 ± 0.043
0.508TrpArg: 0.508 ± 0.034
0.706TrpSer: 0.706 ± 0.039
0.562TrpThr: 0.562 ± 0.031
0.622TrpVal: 0.622 ± 0.035
0.198TrpTrp: 0.198 ± 0.022
0.342TrpTyr: 0.342 ± 0.029
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.307TyrAla: 2.307 ± 0.076
0.301TyrCys: 0.301 ± 0.027
1.92TyrAsp: 1.92 ± 0.069
1.492TyrGlu: 1.492 ± 0.061
1.791TyrPhe: 1.791 ± 0.069
2.2TyrGly: 2.2 ± 0.067
1.046TyrHis: 1.046 ± 0.046
1.999TyrIle: 1.999 ± 0.058
1.309TyrLys: 1.309 ± 0.048
4.399TyrLeu: 4.399 ± 0.102
0.688TyrMet: 0.688 ± 0.042
1.628TyrAsn: 1.628 ± 0.054
1.441TyrPro: 1.441 ± 0.057
3.193TyrGln: 3.193 ± 0.082
1.727TyrArg: 1.727 ± 0.057
1.925TyrSer: 1.925 ± 0.067
1.951TyrThr: 1.951 ± 0.072
2.073TyrVal: 2.073 ± 0.061
0.473TyrTrp: 0.473 ± 0.03
1.509TyrTyr: 1.509 ± 0.061
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1592 proteins (514180 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski