Amino acid dipepetide frequency for Mycobacterium phage Manad

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.03AlaAla: 18.03 ± 1.512
1.153AlaCys: 1.153 ± 0.265
7.332AlaAsp: 7.332 ± 0.552
7.609AlaGlu: 7.609 ± 0.877
2.536AlaPhe: 2.536 ± 0.406
10.652AlaGly: 10.652 ± 1.167
2.398AlaHis: 2.398 ± 0.361
4.611AlaIle: 4.611 ± 0.628
3.597AlaLys: 3.597 ± 0.422
10.606AlaLeu: 10.606 ± 0.955
3.136AlaMet: 3.136 ± 0.372
3.043AlaAsn: 3.043 ± 0.375
6.732AlaPro: 6.732 ± 0.5
4.242AlaGln: 4.242 ± 0.432
8.669AlaArg: 8.669 ± 0.863
5.487AlaSer: 5.487 ± 0.522
7.655AlaThr: 7.655 ± 0.606
9.084AlaVal: 9.084 ± 0.796
1.845AlaTrp: 1.845 ± 0.345
2.951AlaTyr: 2.951 ± 0.355
0.0AlaXaa: 0.0 ± 0.0
Cys
0.784CysAla: 0.784 ± 0.221
0.0CysCys: 0.0 ± 0.0
0.922CysAsp: 0.922 ± 0.217
0.692CysGlu: 0.692 ± 0.226
0.323CysPhe: 0.323 ± 0.12
1.476CysGly: 1.476 ± 0.298
0.323CysHis: 0.323 ± 0.106
0.369CysIle: 0.369 ± 0.12
0.277CysLys: 0.277 ± 0.118
0.553CysLeu: 0.553 ± 0.194
0.231CysMet: 0.231 ± 0.099
0.323CysAsn: 0.323 ± 0.133
0.876CysPro: 0.876 ± 0.248
0.323CysGln: 0.323 ± 0.132
0.784CysArg: 0.784 ± 0.215
0.461CysSer: 0.461 ± 0.132
0.553CysThr: 0.553 ± 0.193
0.784CysVal: 0.784 ± 0.159
0.231CysTrp: 0.231 ± 0.11
0.323CysTyr: 0.323 ± 0.113
0.0CysXaa: 0.0 ± 0.0
Asp
7.009AspAla: 7.009 ± 0.538
0.876AspCys: 0.876 ± 0.243
4.796AspAsp: 4.796 ± 0.649
5.026AspGlu: 5.026 ± 0.668
1.568AspPhe: 1.568 ± 0.226
5.995AspGly: 5.995 ± 0.618
1.199AspHis: 1.199 ± 0.292
3.228AspIle: 3.228 ± 0.385
1.983AspLys: 1.983 ± 0.317
5.626AspLeu: 5.626 ± 0.416
0.968AspMet: 0.968 ± 0.249
2.075AspAsn: 2.075 ± 0.351
5.072AspPro: 5.072 ± 0.487
1.429AspGln: 1.429 ± 0.271
4.15AspArg: 4.15 ± 0.538
2.813AspSer: 2.813 ± 0.394
3.781AspThr: 3.781 ± 0.462
3.966AspVal: 3.966 ± 0.383
1.153AspTrp: 1.153 ± 0.245
1.937AspTyr: 1.937 ± 0.256
0.0AspXaa: 0.0 ± 0.0
Glu
7.194GluAla: 7.194 ± 0.873
0.692GluCys: 0.692 ± 0.21
3.551GluAsp: 3.551 ± 0.6
2.352GluGlu: 2.352 ± 0.336
2.213GluPhe: 2.213 ± 0.349
4.381GluGly: 4.381 ± 0.522
1.245GluHis: 1.245 ± 0.248
3.781GluIle: 3.781 ± 0.534
1.66GluLys: 1.66 ± 0.294
6.41GluLeu: 6.41 ± 0.534
1.153GluMet: 1.153 ± 0.256
1.107GluAsn: 1.107 ± 0.198
3.366GluPro: 3.366 ± 0.525
2.49GluGln: 2.49 ± 0.36
4.381GluArg: 4.381 ± 0.552
1.937GluSer: 1.937 ± 0.281
3.827GluThr: 3.827 ± 0.469
5.81GluVal: 5.81 ± 0.644
0.922GluTrp: 0.922 ± 0.2
1.107GluTyr: 1.107 ± 0.205
0.0GluXaa: 0.0 ± 0.0
Phe
2.997PheAla: 2.997 ± 0.341
0.277PheCys: 0.277 ± 0.118
1.614PheAsp: 1.614 ± 0.253
1.522PheGlu: 1.522 ± 0.263
0.507PhePhe: 0.507 ± 0.129
2.905PheGly: 2.905 ± 0.483
0.461PheHis: 0.461 ± 0.13
0.692PheIle: 0.692 ± 0.184
1.061PheLys: 1.061 ± 0.209
1.476PheLeu: 1.476 ± 0.264
0.323PheMet: 0.323 ± 0.109
0.968PheAsn: 0.968 ± 0.223
1.429PhePro: 1.429 ± 0.266
0.646PheGln: 0.646 ± 0.148
1.614PheArg: 1.614 ± 0.262
1.199PheSer: 1.199 ± 0.281
1.476PheThr: 1.476 ± 0.241
1.522PheVal: 1.522 ± 0.228
0.415PheTrp: 0.415 ± 0.138
0.553PheTyr: 0.553 ± 0.174
0.0PheXaa: 0.0 ± 0.0
Gly
9.315GlyAla: 9.315 ± 1.415
1.153GlyCys: 1.153 ± 0.286
5.534GlyAsp: 5.534 ± 0.581
5.395GlyGlu: 5.395 ± 0.581
2.029GlyPhe: 2.029 ± 0.36
13.188GlyGly: 13.188 ± 2.963
2.029GlyHis: 2.029 ± 0.448
4.75GlyIle: 4.75 ± 0.384
3.551GlyLys: 3.551 ± 0.494
7.747GlyLeu: 7.747 ± 0.865
2.213GlyMet: 2.213 ± 0.322
2.536GlyAsn: 2.536 ± 0.511
4.473GlyPro: 4.473 ± 0.559
3.412GlyGln: 3.412 ± 0.526
5.718GlyArg: 5.718 ± 0.65
5.165GlySer: 5.165 ± 0.618
6.871GlyThr: 6.871 ± 0.824
6.732GlyVal: 6.732 ± 0.691
1.891GlyTrp: 1.891 ± 0.309
2.628GlyTyr: 2.628 ± 0.325
0.0GlyXaa: 0.0 ± 0.0
His
2.029HisAla: 2.029 ± 0.352
0.231HisCys: 0.231 ± 0.117
1.153HisAsp: 1.153 ± 0.257
1.522HisGlu: 1.522 ± 0.297
0.461HisPhe: 0.461 ± 0.138
1.614HisGly: 1.614 ± 0.257
0.876HisHis: 0.876 ± 0.361
1.568HisIle: 1.568 ± 0.322
0.461HisLys: 0.461 ± 0.147
1.337HisLeu: 1.337 ± 0.352
0.415HisMet: 0.415 ± 0.128
0.461HisAsn: 0.461 ± 0.137
1.153HisPro: 1.153 ± 0.259
0.231HisGln: 0.231 ± 0.081
2.075HisArg: 2.075 ± 0.396
0.83HisSer: 0.83 ± 0.199
1.845HisThr: 1.845 ± 0.387
1.061HisVal: 1.061 ± 0.232
0.231HisTrp: 0.231 ± 0.107
0.692HisTyr: 0.692 ± 0.229
0.0HisXaa: 0.0 ± 0.0
Ile
5.81IleAla: 5.81 ± 0.436
0.553IleCys: 0.553 ± 0.153
3.966IleAsp: 3.966 ± 0.453
4.888IleGlu: 4.888 ± 0.559
0.83IlePhe: 0.83 ± 0.189
5.165IleGly: 5.165 ± 0.573
0.738IleHis: 0.738 ± 0.19
1.614IleIle: 1.614 ± 0.309
1.429IleLys: 1.429 ± 0.341
2.951IleLeu: 2.951 ± 0.378
0.784IleMet: 0.784 ± 0.188
1.337IleAsn: 1.337 ± 0.28
2.582IlePro: 2.582 ± 0.377
0.784IleGln: 0.784 ± 0.221
2.167IleArg: 2.167 ± 0.362
2.628IleSer: 2.628 ± 0.324
3.551IleThr: 3.551 ± 0.297
3.09IleVal: 3.09 ± 0.475
0.277IleTrp: 0.277 ± 0.108
0.876IleTyr: 0.876 ± 0.196
0.0IleXaa: 0.0 ± 0.0
Lys
4.335LysAla: 4.335 ± 0.519
0.231LysCys: 0.231 ± 0.117
1.476LysAsp: 1.476 ± 0.22
0.784LysGlu: 0.784 ± 0.277
0.738LysPhe: 0.738 ± 0.17
2.675LysGly: 2.675 ± 0.385
0.507LysHis: 0.507 ± 0.15
1.66LysIle: 1.66 ± 0.245
1.014LysLys: 1.014 ± 0.285
2.813LysLeu: 2.813 ± 0.373
0.415LysMet: 0.415 ± 0.131
0.784LysAsn: 0.784 ± 0.205
1.383LysPro: 1.383 ± 0.335
1.337LysGln: 1.337 ± 0.248
2.075LysArg: 2.075 ± 0.336
1.476LysSer: 1.476 ± 0.261
2.167LysThr: 2.167 ± 0.317
2.767LysVal: 2.767 ± 0.282
0.369LysTrp: 0.369 ± 0.128
0.646LysTyr: 0.646 ± 0.157
0.0LysXaa: 0.0 ± 0.0
Leu
11.574LeuAla: 11.574 ± 0.9
0.968LeuCys: 0.968 ± 0.222
6.087LeuAsp: 6.087 ± 0.673
2.997LeuGlu: 2.997 ± 0.339
1.983LeuPhe: 1.983 ± 0.366
7.378LeuGly: 7.378 ± 1.117
1.153LeuHis: 1.153 ± 0.215
3.735LeuIle: 3.735 ± 0.476
2.582LeuLys: 2.582 ± 0.447
5.395LeuLeu: 5.395 ± 0.658
1.66LeuMet: 1.66 ± 0.243
3.412LeuAsn: 3.412 ± 0.502
6.041LeuPro: 6.041 ± 0.499
1.614LeuGln: 1.614 ± 0.291
4.15LeuArg: 4.15 ± 0.552
3.92LeuSer: 3.92 ± 0.429
7.055LeuThr: 7.055 ± 0.529
4.888LeuVal: 4.888 ± 0.502
1.245LeuTrp: 1.245 ± 0.251
1.706LeuTyr: 1.706 ± 0.323
0.0LeuXaa: 0.0 ± 0.0
Met
2.582MetAla: 2.582 ± 0.356
0.277MetCys: 0.277 ± 0.109
0.599MetAsp: 0.599 ± 0.161
0.692MetGlu: 0.692 ± 0.144
0.784MetPhe: 0.784 ± 0.182
1.337MetGly: 1.337 ± 0.235
0.461MetHis: 0.461 ± 0.205
0.876MetIle: 0.876 ± 0.188
0.599MetLys: 0.599 ± 0.154
1.383MetLeu: 1.383 ± 0.231
0.461MetMet: 0.461 ± 0.138
0.461MetAsn: 0.461 ± 0.151
1.522MetPro: 1.522 ± 0.25
0.415MetGln: 0.415 ± 0.124
1.245MetArg: 1.245 ± 0.227
1.891MetSer: 1.891 ± 0.316
2.721MetThr: 2.721 ± 0.299
1.522MetVal: 1.522 ± 0.264
0.507MetTrp: 0.507 ± 0.158
0.553MetTyr: 0.553 ± 0.177
0.0MetXaa: 0.0 ± 0.0
Asn
3.505AsnAla: 3.505 ± 0.4
0.277AsnCys: 0.277 ± 0.107
1.891AsnAsp: 1.891 ± 0.303
1.245AsnGlu: 1.245 ± 0.224
0.369AsnPhe: 0.369 ± 0.126
3.551AsnGly: 3.551 ± 0.481
0.646AsnHis: 0.646 ± 0.197
1.291AsnIle: 1.291 ± 0.311
1.153AsnLys: 1.153 ± 0.284
1.891AsnLeu: 1.891 ± 0.273
0.461AsnMet: 0.461 ± 0.1
1.291AsnAsn: 1.291 ± 0.288
1.798AsnPro: 1.798 ± 0.28
0.415AsnGln: 0.415 ± 0.133
2.029AsnArg: 2.029 ± 0.372
1.798AsnSer: 1.798 ± 0.303
2.444AsnThr: 2.444 ± 0.406
1.706AsnVal: 1.706 ± 0.22
0.369AsnTrp: 0.369 ± 0.158
0.876AsnTyr: 0.876 ± 0.187
0.0AsnXaa: 0.0 ± 0.0
Pro
7.332ProAla: 7.332 ± 0.651
0.369ProCys: 0.369 ± 0.112
4.242ProAsp: 4.242 ± 0.574
5.487ProGlu: 5.487 ± 0.699
1.476ProPhe: 1.476 ± 0.227
6.225ProGly: 6.225 ± 0.801
1.383ProHis: 1.383 ± 0.332
2.536ProIle: 2.536 ± 0.414
1.245ProLys: 1.245 ± 0.215
3.458ProLeu: 3.458 ± 0.4
1.245ProMet: 1.245 ± 0.234
2.029ProAsn: 2.029 ± 0.329
4.75ProPro: 4.75 ± 0.688
1.706ProGln: 1.706 ± 0.256
3.827ProArg: 3.827 ± 0.524
2.444ProSer: 2.444 ± 0.34
3.966ProThr: 3.966 ± 0.335
5.165ProVal: 5.165 ± 0.52
1.291ProTrp: 1.291 ± 0.244
0.968ProTyr: 0.968 ± 0.23
0.0ProXaa: 0.0 ± 0.0
Gln
4.104GlnAla: 4.104 ± 0.649
0.231GlnCys: 0.231 ± 0.108
1.061GlnAsp: 1.061 ± 0.195
0.876GlnGlu: 0.876 ± 0.196
0.968GlnPhe: 0.968 ± 0.188
2.213GlnGly: 2.213 ± 0.397
0.876GlnHis: 0.876 ± 0.204
2.075GlnIle: 2.075 ± 0.319
0.784GlnLys: 0.784 ± 0.202
2.398GlnLeu: 2.398 ± 0.365
0.553GlnMet: 0.553 ± 0.153
0.738GlnAsn: 0.738 ± 0.199
1.614GlnPro: 1.614 ± 0.257
1.752GlnGln: 1.752 ± 0.26
2.859GlnArg: 2.859 ± 0.45
1.66GlnSer: 1.66 ± 0.305
2.398GlnThr: 2.398 ± 0.318
2.075GlnVal: 2.075 ± 0.275
0.692GlnTrp: 0.692 ± 0.202
0.738GlnTyr: 0.738 ± 0.205
0.0GlnXaa: 0.0 ± 0.0
Arg
7.286ArgAla: 7.286 ± 0.762
0.646ArgCys: 0.646 ± 0.175
4.104ArgAsp: 4.104 ± 0.418
5.119ArgGlu: 5.119 ± 0.685
1.568ArgPhe: 1.568 ± 0.252
3.92ArgGly: 3.92 ± 0.488
1.614ArgHis: 1.614 ± 0.394
2.536ArgIle: 2.536 ± 0.319
1.798ArgLys: 1.798 ± 0.258
5.856ArgLeu: 5.856 ± 0.51
1.66ArgMet: 1.66 ± 0.452
2.306ArgAsn: 2.306 ± 0.36
4.058ArgPro: 4.058 ± 0.57
2.675ArgGln: 2.675 ± 0.378
6.179ArgArg: 6.179 ± 0.716
2.628ArgSer: 2.628 ± 0.277
4.012ArgThr: 4.012 ± 0.434
5.349ArgVal: 5.349 ± 0.624
1.752ArgTrp: 1.752 ± 0.314
2.352ArgTyr: 2.352 ± 0.37
0.0ArgXaa: 0.0 ± 0.0
Ser
4.934SerAla: 4.934 ± 0.557
0.507SerCys: 0.507 ± 0.156
3.32SerAsp: 3.32 ± 0.33
2.26SerGlu: 2.26 ± 0.387
0.784SerPhe: 0.784 ± 0.243
5.995SerGly: 5.995 ± 0.764
0.738SerHis: 0.738 ± 0.193
1.752SerIle: 1.752 ± 0.298
1.245SerLys: 1.245 ± 0.224
4.058SerLeu: 4.058 ± 0.705
1.337SerMet: 1.337 ± 0.215
1.153SerAsn: 1.153 ± 0.225
3.274SerPro: 3.274 ± 0.388
1.937SerGln: 1.937 ± 0.265
3.274SerArg: 3.274 ± 0.278
2.352SerSer: 2.352 ± 0.412
3.966SerThr: 3.966 ± 0.543
3.873SerVal: 3.873 ± 0.43
1.107SerTrp: 1.107 ± 0.177
1.429SerTyr: 1.429 ± 0.228
0.0SerXaa: 0.0 ± 0.0
Thr
8.9ThrAla: 8.9 ± 0.524
0.507ThrCys: 0.507 ± 0.164
5.072ThrAsp: 5.072 ± 0.503
3.366ThrGlu: 3.366 ± 0.409
1.568ThrPhe: 1.568 ± 0.289
6.963ThrGly: 6.963 ± 0.487
1.061ThrHis: 1.061 ± 0.245
3.551ThrIle: 3.551 ± 0.462
2.398ThrLys: 2.398 ± 0.277
5.58ThrLeu: 5.58 ± 0.523
1.706ThrMet: 1.706 ± 0.284
1.798ThrAsn: 1.798 ± 0.377
5.119ThrPro: 5.119 ± 0.526
1.845ThrGln: 1.845 ± 0.333
3.735ThrArg: 3.735 ± 0.422
4.104ThrSer: 4.104 ± 0.366
4.012ThrThr: 4.012 ± 0.61
6.548ThrVal: 6.548 ± 0.586
1.199ThrTrp: 1.199 ± 0.28
1.752ThrTyr: 1.752 ± 0.292
0.0ThrXaa: 0.0 ± 0.0
Val
8.439ValAla: 8.439 ± 0.498
0.876ValCys: 0.876 ± 0.221
4.473ValAsp: 4.473 ± 0.432
5.81ValGlu: 5.81 ± 0.646
1.614ValPhe: 1.614 ± 0.26
7.562ValGly: 7.562 ± 0.596
1.614ValHis: 1.614 ± 0.26
3.412ValIle: 3.412 ± 0.349
1.891ValLys: 1.891 ± 0.271
6.594ValLeu: 6.594 ± 0.506
1.291ValMet: 1.291 ± 0.282
2.121ValAsn: 2.121 ± 0.281
3.92ValPro: 3.92 ± 0.365
1.798ValGln: 1.798 ± 0.267
4.888ValArg: 4.888 ± 0.518
4.058ValSer: 4.058 ± 0.406
5.441ValThr: 5.441 ± 0.563
6.271ValVal: 6.271 ± 0.733
1.983ValTrp: 1.983 ± 0.303
1.891ValTyr: 1.891 ± 0.39
0.0ValXaa: 0.0 ± 0.0
Trp
2.26TrpAla: 2.26 ± 0.37
0.415TrpCys: 0.415 ± 0.14
1.245TrpAsp: 1.245 ± 0.261
0.553TrpGlu: 0.553 ± 0.141
0.738TrpPhe: 0.738 ± 0.202
1.061TrpGly: 1.061 ± 0.236
0.461TrpHis: 0.461 ± 0.139
0.968TrpIle: 0.968 ± 0.212
0.277TrpLys: 0.277 ± 0.083
1.429TrpLeu: 1.429 ± 0.244
0.461TrpMet: 0.461 ± 0.131
0.277TrpAsn: 0.277 ± 0.097
0.83TrpPro: 0.83 ± 0.179
0.692TrpGln: 0.692 ± 0.199
1.291TrpArg: 1.291 ± 0.218
1.337TrpSer: 1.337 ± 0.273
1.383TrpThr: 1.383 ± 0.302
1.568TrpVal: 1.568 ± 0.36
0.461TrpTrp: 0.461 ± 0.151
0.461TrpTyr: 0.461 ± 0.155
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.997TyrAla: 2.997 ± 0.336
0.369TyrCys: 0.369 ± 0.142
2.213TyrAsp: 2.213 ± 0.27
1.383TyrGlu: 1.383 ± 0.267
0.599TyrPhe: 0.599 ± 0.182
2.075TyrGly: 2.075 ± 0.406
0.461TyrHis: 0.461 ± 0.132
1.014TyrIle: 1.014 ± 0.206
0.646TyrLys: 0.646 ± 0.179
1.983TyrLeu: 1.983 ± 0.384
0.369TyrMet: 0.369 ± 0.14
0.784TyrAsn: 0.784 ± 0.149
1.153TyrPro: 1.153 ± 0.234
0.922TyrGln: 0.922 ± 0.231
2.306TyrArg: 2.306 ± 0.338
1.107TyrSer: 1.107 ± 0.199
1.66TyrThr: 1.66 ± 0.256
2.167TyrVal: 2.167 ± 0.254
0.231TyrTrp: 0.231 ± 0.092
0.599TyrTyr: 0.599 ± 0.159
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 101 proteins (21687 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski