Amino acid dipepetide frequency for Exiguobacterium phage vB_EalM-137

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.641AlaAla: 5.641 ± 0.756
0.397AlaCys: 0.397 ± 0.156
3.655AlaAsp: 3.655 ± 0.641
5.244AlaGlu: 5.244 ± 0.774
3.178AlaPhe: 3.178 ± 0.853
5.244AlaGly: 5.244 ± 1.004
1.033AlaHis: 1.033 ± 0.273
4.688AlaIle: 4.688 ± 0.762
5.323AlaLys: 5.323 ± 0.583
6.436AlaLeu: 6.436 ± 0.654
2.145AlaMet: 2.145 ± 0.35
4.211AlaAsn: 4.211 ± 0.676
3.019AlaPro: 3.019 ± 0.659
2.701AlaGln: 2.701 ± 0.5
4.052AlaArg: 4.052 ± 0.559
4.688AlaSer: 4.688 ± 0.739
3.973AlaThr: 3.973 ± 0.484
6.436AlaVal: 6.436 ± 0.805
0.795AlaTrp: 0.795 ± 0.272
2.622AlaTyr: 2.622 ± 0.474
0.0AlaXaa: 0.0 ± 0.0
Cys
0.159CysAla: 0.159 ± 0.114
0.079CysCys: 0.079 ± 0.077
0.318CysAsp: 0.318 ± 0.207
0.318CysGlu: 0.318 ± 0.171
0.079CysPhe: 0.079 ± 0.087
0.397CysGly: 0.397 ± 0.195
0.079CysHis: 0.079 ± 0.077
0.238CysIle: 0.238 ± 0.135
0.159CysLys: 0.159 ± 0.159
0.477CysLeu: 0.477 ± 0.234
0.159CysMet: 0.159 ± 0.093
0.079CysAsn: 0.079 ± 0.062
0.238CysPro: 0.238 ± 0.133
0.159CysGln: 0.159 ± 0.114
0.238CysArg: 0.238 ± 0.124
0.318CysSer: 0.318 ± 0.173
0.159CysThr: 0.159 ± 0.103
0.318CysVal: 0.318 ± 0.145
0.0CysTrp: 0.0 ± 0.0
0.159CysTyr: 0.159 ± 0.131
0.0CysXaa: 0.0 ± 0.0
Asp
5.085AspAla: 5.085 ± 0.701
0.318AspCys: 0.318 ± 0.163
4.052AspAsp: 4.052 ± 0.741
4.926AspGlu: 4.926 ± 0.619
2.543AspPhe: 2.543 ± 0.465
4.529AspGly: 4.529 ± 0.601
1.033AspHis: 1.033 ± 0.394
3.655AspIle: 3.655 ± 0.581
3.655AspLys: 3.655 ± 0.538
5.006AspLeu: 5.006 ± 0.588
1.351AspMet: 1.351 ± 0.301
1.827AspAsn: 1.827 ± 0.358
2.86AspPro: 2.86 ± 0.63
1.669AspGln: 1.669 ± 0.323
2.86AspArg: 2.86 ± 0.456
2.781AspSer: 2.781 ± 0.428
2.781AspThr: 2.781 ± 0.46
4.767AspVal: 4.767 ± 0.824
1.033AspTrp: 1.033 ± 0.256
1.748AspTyr: 1.748 ± 0.378
0.0AspXaa: 0.0 ± 0.0
Glu
5.562GluAla: 5.562 ± 0.612
0.238GluCys: 0.238 ± 0.147
3.893GluAsp: 3.893 ± 0.614
6.118GluGlu: 6.118 ± 1.105
2.384GluPhe: 2.384 ± 0.349
4.37GluGly: 4.37 ± 0.667
1.271GluHis: 1.271 ± 0.317
4.926GluIle: 4.926 ± 0.748
5.562GluLys: 5.562 ± 0.958
7.389GluLeu: 7.389 ± 0.963
2.225GluMet: 2.225 ± 0.479
3.496GluAsn: 3.496 ± 0.652
2.225GluPro: 2.225 ± 0.468
4.052GluGln: 4.052 ± 0.691
5.085GluArg: 5.085 ± 0.731
5.244GluSer: 5.244 ± 0.637
4.29GluThr: 4.29 ± 0.657
4.926GluVal: 4.926 ± 0.536
1.51GluTrp: 1.51 ± 0.413
2.701GluTyr: 2.701 ± 0.543
0.0GluXaa: 0.0 ± 0.0
Phe
1.986PheAla: 1.986 ± 0.412
0.159PheCys: 0.159 ± 0.17
2.701PheAsp: 2.701 ± 0.404
4.132PheGlu: 4.132 ± 0.475
1.033PhePhe: 1.033 ± 0.254
2.701PheGly: 2.701 ± 0.478
0.477PheHis: 0.477 ± 0.218
2.384PheIle: 2.384 ± 0.444
2.384PheLys: 2.384 ± 0.459
2.94PheLeu: 2.94 ± 0.42
0.318PheMet: 0.318 ± 0.227
2.145PheAsn: 2.145 ± 0.486
0.795PhePro: 0.795 ± 0.318
0.795PheGln: 0.795 ± 0.244
2.463PheArg: 2.463 ± 0.366
2.622PheSer: 2.622 ± 0.625
2.225PheThr: 2.225 ± 0.435
3.258PheVal: 3.258 ± 0.642
0.397PheTrp: 0.397 ± 0.252
1.351PheTyr: 1.351 ± 0.293
0.0PheXaa: 0.0 ± 0.0
Gly
5.959GlyAla: 5.959 ± 1.143
0.079GlyCys: 0.079 ± 0.083
4.211GlyAsp: 4.211 ± 0.61
4.132GlyGlu: 4.132 ± 0.549
2.622GlyPhe: 2.622 ± 0.511
5.323GlyGly: 5.323 ± 0.725
0.795GlyHis: 0.795 ± 0.189
5.244GlyIle: 5.244 ± 0.988
5.085GlyLys: 5.085 ± 0.567
6.118GlyLeu: 6.118 ± 1.096
1.907GlyMet: 1.907 ± 0.379
2.94GlyAsn: 2.94 ± 0.676
1.907GlyPro: 1.907 ± 0.353
3.099GlyGln: 3.099 ± 0.556
3.893GlyArg: 3.893 ± 0.7
4.608GlySer: 4.608 ± 0.746
3.814GlyThr: 3.814 ± 0.458
5.164GlyVal: 5.164 ± 0.536
0.953GlyTrp: 0.953 ± 0.238
3.019GlyTyr: 3.019 ± 0.418
0.0GlyXaa: 0.0 ± 0.0
His
0.874HisAla: 0.874 ± 0.285
0.238HisCys: 0.238 ± 0.14
0.874HisAsp: 0.874 ± 0.246
1.033HisGlu: 1.033 ± 0.294
1.271HisPhe: 1.271 ± 0.364
1.112HisGly: 1.112 ± 0.27
0.159HisHis: 0.159 ± 0.112
1.271HisIle: 1.271 ± 0.385
1.192HisLys: 1.192 ± 0.271
1.827HisLeu: 1.827 ± 0.352
0.318HisMet: 0.318 ± 0.148
0.318HisAsn: 0.318 ± 0.162
0.874HisPro: 0.874 ± 0.241
0.477HisGln: 0.477 ± 0.183
0.953HisArg: 0.953 ± 0.327
0.715HisSer: 0.715 ± 0.21
1.112HisThr: 1.112 ± 0.291
1.112HisVal: 1.112 ± 0.287
0.238HisTrp: 0.238 ± 0.147
0.318HisTyr: 0.318 ± 0.171
0.0HisXaa: 0.0 ± 0.0
Ile
5.085IleAla: 5.085 ± 0.614
0.397IleCys: 0.397 ± 0.181
5.006IleAsp: 5.006 ± 0.571
5.482IleGlu: 5.482 ± 0.701
1.748IlePhe: 1.748 ± 0.358
4.847IleGly: 4.847 ± 0.578
1.192IleHis: 1.192 ± 0.336
3.416IleIle: 3.416 ± 0.529
5.085IleLys: 5.085 ± 0.663
4.767IleLeu: 4.767 ± 0.667
1.192IleMet: 1.192 ± 0.341
3.416IleAsn: 3.416 ± 0.566
2.94IlePro: 2.94 ± 0.412
2.304IleGln: 2.304 ± 0.415
3.655IleArg: 3.655 ± 0.558
4.132IleSer: 4.132 ± 0.617
2.622IleThr: 2.622 ± 0.521
4.608IleVal: 4.608 ± 0.565
0.556IleTrp: 0.556 ± 0.201
2.066IleTyr: 2.066 ± 0.419
0.0IleXaa: 0.0 ± 0.0
Lys
4.767LysAla: 4.767 ± 0.606
0.238LysCys: 0.238 ± 0.124
4.608LysAsp: 4.608 ± 0.607
5.88LysGlu: 5.88 ± 0.755
1.43LysPhe: 1.43 ± 0.36
5.88LysGly: 5.88 ± 0.618
1.271LysHis: 1.271 ± 0.317
4.767LysIle: 4.767 ± 0.68
5.006LysLys: 5.006 ± 1.13
4.608LysLeu: 4.608 ± 0.579
2.622LysMet: 2.622 ± 0.418
2.781LysAsn: 2.781 ± 0.43
2.701LysPro: 2.701 ± 0.543
2.066LysGln: 2.066 ± 0.345
4.132LysArg: 4.132 ± 0.572
4.052LysSer: 4.052 ± 0.566
4.767LysThr: 4.767 ± 0.779
5.403LysVal: 5.403 ± 0.731
1.192LysTrp: 1.192 ± 0.356
1.589LysTyr: 1.589 ± 0.418
0.0LysXaa: 0.0 ± 0.0
Leu
6.277LeuAla: 6.277 ± 0.645
0.159LeuCys: 0.159 ± 0.117
6.515LeuAsp: 6.515 ± 0.658
6.912LeuGlu: 6.912 ± 1.025
2.225LeuPhe: 2.225 ± 0.357
5.959LeuGly: 5.959 ± 1.022
1.192LeuHis: 1.192 ± 0.302
4.847LeuIle: 4.847 ± 0.654
5.641LeuLys: 5.641 ± 0.541
7.469LeuLeu: 7.469 ± 0.868
1.827LeuMet: 1.827 ± 0.51
4.688LeuAsn: 4.688 ± 0.46
3.337LeuPro: 3.337 ± 0.503
3.178LeuGln: 3.178 ± 0.483
4.211LeuArg: 4.211 ± 0.692
6.833LeuSer: 6.833 ± 0.64
5.006LeuThr: 5.006 ± 0.551
4.608LeuVal: 4.608 ± 0.622
0.556LeuTrp: 0.556 ± 0.222
1.827LeuTyr: 1.827 ± 0.377
0.0LeuXaa: 0.0 ± 0.0
Met
3.814MetAla: 3.814 ± 0.729
0.159MetCys: 0.159 ± 0.113
1.112MetAsp: 1.112 ± 0.416
1.51MetGlu: 1.51 ± 0.326
1.033MetPhe: 1.033 ± 0.299
1.51MetGly: 1.51 ± 0.622
0.477MetHis: 0.477 ± 0.178
1.907MetIle: 1.907 ± 0.364
1.748MetLys: 1.748 ± 0.318
2.145MetLeu: 2.145 ± 0.564
1.033MetMet: 1.033 ± 0.343
1.589MetAsn: 1.589 ± 0.363
0.874MetPro: 0.874 ± 0.235
0.715MetGln: 0.715 ± 0.18
1.827MetArg: 1.827 ± 0.385
2.066MetSer: 2.066 ± 0.423
2.225MetThr: 2.225 ± 0.354
1.669MetVal: 1.669 ± 0.415
0.079MetTrp: 0.079 ± 0.078
0.318MetTyr: 0.318 ± 0.162
0.0MetXaa: 0.0 ± 0.0
Asn
3.496AsnAla: 3.496 ± 0.57
0.159AsnCys: 0.159 ± 0.109
2.384AsnAsp: 2.384 ± 0.449
3.973AsnGlu: 3.973 ± 0.752
1.907AsnPhe: 1.907 ± 0.33
5.164AsnGly: 5.164 ± 0.769
0.874AsnHis: 0.874 ± 0.255
3.178AsnIle: 3.178 ± 0.412
2.701AsnLys: 2.701 ± 0.474
2.145AsnLeu: 2.145 ± 0.444
1.907AsnMet: 1.907 ± 0.589
1.589AsnAsn: 1.589 ± 0.303
2.543AsnPro: 2.543 ± 0.382
1.033AsnGln: 1.033 ± 0.27
2.622AsnArg: 2.622 ± 0.502
1.589AsnSer: 1.589 ± 0.351
2.304AsnThr: 2.304 ± 0.559
3.416AsnVal: 3.416 ± 0.489
0.795AsnTrp: 0.795 ± 0.215
1.589AsnTyr: 1.589 ± 0.379
0.0AsnXaa: 0.0 ± 0.0
Pro
1.907ProAla: 1.907 ± 0.328
0.079ProCys: 0.079 ± 0.085
1.669ProAsp: 1.669 ± 0.39
3.178ProGlu: 3.178 ± 0.442
1.112ProPhe: 1.112 ± 0.335
2.781ProGly: 2.781 ± 0.528
0.159ProHis: 0.159 ± 0.095
2.86ProIle: 2.86 ± 0.472
3.099ProLys: 3.099 ± 0.568
2.543ProLeu: 2.543 ± 0.427
1.192ProMet: 1.192 ± 0.298
1.748ProAsn: 1.748 ± 0.39
1.271ProPro: 1.271 ± 0.35
1.986ProGln: 1.986 ± 0.364
1.43ProArg: 1.43 ± 0.404
2.94ProSer: 2.94 ± 0.573
2.463ProThr: 2.463 ± 0.457
2.225ProVal: 2.225 ± 0.388
0.477ProTrp: 0.477 ± 0.179
1.192ProTyr: 1.192 ± 0.311
0.0ProXaa: 0.0 ± 0.0
Gln
2.701GlnAla: 2.701 ± 0.497
0.238GlnCys: 0.238 ± 0.173
1.112GlnAsp: 1.112 ± 0.303
2.94GlnGlu: 2.94 ± 0.408
1.51GlnPhe: 1.51 ± 0.457
1.986GlnGly: 1.986 ± 0.374
0.318GlnHis: 0.318 ± 0.152
2.781GlnIle: 2.781 ± 0.417
3.337GlnLys: 3.337 ± 0.569
3.416GlnLeu: 3.416 ± 0.565
1.351GlnMet: 1.351 ± 0.283
0.874GlnAsn: 0.874 ± 0.252
1.192GlnPro: 1.192 ± 0.297
1.827GlnGln: 1.827 ± 0.356
1.986GlnArg: 1.986 ± 0.348
2.701GlnSer: 2.701 ± 0.476
2.066GlnThr: 2.066 ± 0.389
2.622GlnVal: 2.622 ± 0.417
0.238GlnTrp: 0.238 ± 0.134
1.43GlnTyr: 1.43 ± 0.25
0.0GlnXaa: 0.0 ± 0.0
Arg
3.734ArgAla: 3.734 ± 0.649
0.079ArgCys: 0.079 ± 0.083
2.94ArgAsp: 2.94 ± 0.454
4.132ArgGlu: 4.132 ± 0.651
3.337ArgPhe: 3.337 ± 0.428
2.304ArgGly: 2.304 ± 0.487
1.351ArgHis: 1.351 ± 0.394
4.29ArgIle: 4.29 ± 0.602
3.973ArgLys: 3.973 ± 0.562
5.641ArgLeu: 5.641 ± 0.761
1.271ArgMet: 1.271 ± 0.333
3.258ArgAsn: 3.258 ± 0.639
1.43ArgPro: 1.43 ± 0.458
2.384ArgGln: 2.384 ± 0.523
3.893ArgArg: 3.893 ± 0.533
2.86ArgSer: 2.86 ± 0.488
2.86ArgThr: 2.86 ± 0.482
4.29ArgVal: 4.29 ± 0.572
0.795ArgTrp: 0.795 ± 0.227
1.748ArgTyr: 1.748 ± 0.383
0.0ArgXaa: 0.0 ± 0.0
Ser
4.608SerAla: 4.608 ± 0.51
0.477SerCys: 0.477 ± 0.25
3.496SerAsp: 3.496 ± 0.491
4.688SerGlu: 4.688 ± 0.514
2.463SerPhe: 2.463 ± 0.374
6.118SerGly: 6.118 ± 0.914
1.033SerHis: 1.033 ± 0.292
4.211SerIle: 4.211 ± 0.642
3.734SerLys: 3.734 ± 0.516
4.449SerLeu: 4.449 ± 0.674
2.543SerMet: 2.543 ± 0.499
2.86SerAsn: 2.86 ± 0.419
2.145SerPro: 2.145 ± 0.374
2.145SerGln: 2.145 ± 0.456
4.052SerArg: 4.052 ± 0.507
4.529SerSer: 4.529 ± 0.671
3.496SerThr: 3.496 ± 0.615
4.847SerVal: 4.847 ± 0.511
0.556SerTrp: 0.556 ± 0.191
2.145SerTyr: 2.145 ± 0.348
0.0SerXaa: 0.0 ± 0.0
Thr
4.926ThrAla: 4.926 ± 0.723
0.079ThrCys: 0.079 ± 0.077
3.019ThrAsp: 3.019 ± 0.509
3.893ThrGlu: 3.893 ± 0.668
2.701ThrPhe: 2.701 ± 0.547
3.019ThrGly: 3.019 ± 0.429
1.033ThrHis: 1.033 ± 0.244
3.019ThrIle: 3.019 ± 0.465
3.416ThrLys: 3.416 ± 0.599
6.197ThrLeu: 6.197 ± 0.919
1.589ThrMet: 1.589 ± 0.453
2.543ThrAsn: 2.543 ± 0.382
2.384ThrPro: 2.384 ± 0.349
1.51ThrGln: 1.51 ± 0.312
2.701ThrArg: 2.701 ± 0.518
4.37ThrSer: 4.37 ± 0.608
3.814ThrThr: 3.814 ± 0.637
5.085ThrVal: 5.085 ± 0.524
0.715ThrTrp: 0.715 ± 0.278
1.748ThrTyr: 1.748 ± 0.401
0.0ThrXaa: 0.0 ± 0.0
Val
6.197ValAla: 6.197 ± 0.548
0.397ValCys: 0.397 ± 0.161
4.449ValAsp: 4.449 ± 0.693
4.926ValGlu: 4.926 ± 0.756
2.701ValPhe: 2.701 ± 0.542
5.164ValGly: 5.164 ± 0.818
1.589ValHis: 1.589 ± 0.35
4.529ValIle: 4.529 ± 0.657
5.403ValLys: 5.403 ± 0.662
6.118ValLeu: 6.118 ± 0.633
1.748ValMet: 1.748 ± 0.296
3.416ValAsn: 3.416 ± 0.494
2.463ValPro: 2.463 ± 0.416
2.463ValGln: 2.463 ± 0.429
3.099ValArg: 3.099 ± 0.426
4.767ValSer: 4.767 ± 0.736
5.323ValThr: 5.323 ± 0.669
5.88ValVal: 5.88 ± 0.698
1.192ValTrp: 1.192 ± 0.249
1.986ValTyr: 1.986 ± 0.302
0.0ValXaa: 0.0 ± 0.0
Trp
0.715TrpAla: 0.715 ± 0.221
0.0TrpCys: 0.0 ± 0.0
0.556TrpAsp: 0.556 ± 0.275
0.953TrpGlu: 0.953 ± 0.223
0.715TrpPhe: 0.715 ± 0.293
0.477TrpGly: 0.477 ± 0.242
0.238TrpHis: 0.238 ± 0.147
0.715TrpIle: 0.715 ± 0.192
1.43TrpLys: 1.43 ± 0.459
1.033TrpLeu: 1.033 ± 0.334
0.397TrpMet: 0.397 ± 0.144
0.556TrpAsn: 0.556 ± 0.167
0.079TrpPro: 0.079 ± 0.083
0.556TrpGln: 0.556 ± 0.221
1.192TrpArg: 1.192 ± 0.295
0.715TrpSer: 0.715 ± 0.205
0.715TrpThr: 0.715 ± 0.23
1.033TrpVal: 1.033 ± 0.251
0.397TrpTrp: 0.397 ± 0.19
0.397TrpTyr: 0.397 ± 0.201
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.986TyrAla: 1.986 ± 0.382
0.159TyrCys: 0.159 ± 0.113
1.986TyrAsp: 1.986 ± 0.301
3.099TyrGlu: 3.099 ± 0.553
1.271TyrPhe: 1.271 ± 0.257
1.986TyrGly: 1.986 ± 0.363
0.715TyrHis: 0.715 ± 0.28
1.589TyrIle: 1.589 ± 0.348
1.827TyrLys: 1.827 ± 0.481
2.543TyrLeu: 2.543 ± 0.381
0.636TyrMet: 0.636 ± 0.199
1.033TyrAsn: 1.033 ± 0.274
1.033TyrPro: 1.033 ± 0.352
1.51TyrGln: 1.51 ± 0.329
2.225TyrArg: 2.225 ± 0.288
2.066TyrSer: 2.066 ± 0.402
1.748TyrThr: 1.748 ± 0.371
2.145TyrVal: 2.145 ± 0.43
0.397TyrTrp: 0.397 ± 0.153
0.795TyrTyr: 0.795 ± 0.282
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 64 proteins (12587 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski