Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus; Aspergillus subgen. Nidulantes; Aspergillus nidulans

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10559 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5B8L6|Q5B8L6_EMENI Uncharacterized protein OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=ANIA_03114 PE=4 SV=1
MM1 pKa = 8.11RR2 pKa = 11.84STQALALALALVPLASAINIISSNDD27 pKa = 3.62DD28 pKa = 2.43GWAEE32 pKa = 3.77INIRR36 pKa = 11.84QLFSTLTSAGHH47 pKa = 5.41SVVLSAPAEE56 pKa = 4.18NKK58 pKa = 10.2SGSGMSAWDD67 pKa = 3.66LTRR70 pKa = 11.84QKK72 pKa = 9.69TPANQTSCPADD83 pKa = 3.46SGSYY87 pKa = 10.48GSNEE91 pKa = 3.64TDD93 pKa = 4.02PRR95 pKa = 11.84LNWVNSYY102 pKa = 10.12PVTSIAYY109 pKa = 9.74GIDD112 pKa = 3.21TLSPQFFDD120 pKa = 4.71GPPDD124 pKa = 3.64LAVSGPNVGSNLGLAVYY141 pKa = 10.24IAGTVGAANYY151 pKa = 8.93AATTGGIPAIAFSGADD167 pKa = 3.55GSSTAWDD174 pKa = 3.92AEE176 pKa = 4.35VPAYY180 pKa = 10.66SSIYY184 pKa = 10.46AEE186 pKa = 4.06LAAKK190 pKa = 9.83VVEE193 pKa = 4.32RR194 pKa = 11.84VVSGGTPYY202 pKa = 10.99LPDD205 pKa = 4.47DD206 pKa = 3.1VWLNVNFPSVEE217 pKa = 4.09GCNTANDD224 pKa = 3.95FSFVLSRR231 pKa = 11.84ILTALPLVTDD241 pKa = 5.18DD242 pKa = 5.85DD243 pKa = 4.93VEE245 pKa = 4.83TCGSTRR251 pKa = 11.84LPTEE255 pKa = 3.82NDD257 pKa = 3.39VVDD260 pKa = 3.51TDD262 pKa = 3.53GCYY265 pKa = 10.78VSISVGAADD274 pKa = 5.63KK275 pKa = 11.21SDD277 pKa = 4.14ADD279 pKa = 3.54ATLQGIVLEE288 pKa = 4.34KK289 pKa = 10.95LGDD292 pKa = 4.02LLTCLPP298 pKa = 4.48

Molecular weight: 30.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C8VP63|C8VP63_EMENI Ubiquitin conjugating enzyme Ubc8 putative (AFU_orthologue AFUA_6G09160) OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=ANIA_01761 PE=3 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.45QKK12 pKa = 9.84LAKK15 pKa = 9.55AQKK18 pKa = 8.59QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.14WRR45 pKa = 11.84KK46 pKa = 7.41TRR48 pKa = 11.84LGII51 pKa = 4.46

Molecular weight: 6.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10558

1

10559

5092340

9

7214

482.3

53.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.551 ± 0.021

1.258 ± 0.009

5.565 ± 0.015

6.188 ± 0.024

3.717 ± 0.014

6.788 ± 0.019

2.369 ± 0.011

5.012 ± 0.016

4.566 ± 0.018

9.162 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.061 ± 0.009

3.679 ± 0.011

5.987 ± 0.026

4.02 ± 0.018

6.252 ± 0.018

8.38 ± 0.026

5.962 ± 0.014

6.123 ± 0.016

1.463 ± 0.008

2.897 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski