Amino acid dipepetide frequency for Verrucomicrobia bacterium SCGC AG-212-E04

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.361AlaAla: 15.361 ± 0.349
1.086AlaCys: 1.086 ± 0.068
5.654AlaAsp: 5.654 ± 0.154
6.449AlaGlu: 6.449 ± 0.167
4.502AlaPhe: 4.502 ± 0.144
9.586AlaGly: 9.586 ± 0.202
2.016AlaHis: 2.016 ± 0.075
5.716AlaIle: 5.716 ± 0.154
4.467AlaLys: 4.467 ± 0.151
12.741AlaLeu: 12.741 ± 0.245
2.874AlaMet: 2.874 ± 0.116
3.34AlaAsn: 3.34 ± 0.104
5.716AlaPro: 5.716 ± 0.246
4.007AlaGln: 4.007 ± 0.119
8.224AlaArg: 8.224 ± 0.191
6.095AlaSer: 6.095 ± 0.173
6.336AlaThr: 6.336 ± 0.178
8.38AlaVal: 8.38 ± 0.153
1.703AlaTrp: 1.703 ± 0.086
2.702AlaTyr: 2.702 ± 0.089
0.0AlaXaa: 0.0 ± 0.0
Cys
1.005CysAla: 1.005 ± 0.064
0.113CysCys: 0.113 ± 0.021
0.542CysAsp: 0.542 ± 0.048
0.498CysGlu: 0.498 ± 0.048
0.416CysPhe: 0.416 ± 0.035
0.842CysGly: 0.842 ± 0.053
0.182CysHis: 0.182 ± 0.026
0.394CysIle: 0.394 ± 0.039
0.21CysLys: 0.21 ± 0.032
0.996CysLeu: 0.996 ± 0.057
0.147CysMet: 0.147 ± 0.023
0.2CysAsn: 0.2 ± 0.027
0.501CysPro: 0.501 ± 0.043
0.235CysGln: 0.235 ± 0.028
0.579CysArg: 0.579 ± 0.044
0.432CysSer: 0.432 ± 0.042
0.416CysThr: 0.416 ± 0.044
0.711CysVal: 0.711 ± 0.053
0.135CysTrp: 0.135 ± 0.022
0.25CysTyr: 0.25 ± 0.027
0.0CysXaa: 0.0 ± 0.0
Asp
5.982AspAla: 5.982 ± 0.139
0.451AspCys: 0.451 ± 0.039
2.555AspAsp: 2.555 ± 0.101
3.137AspGlu: 3.137 ± 0.114
2.451AspPhe: 2.451 ± 0.094
4.621AspGly: 4.621 ± 0.139
1.042AspHis: 1.042 ± 0.059
1.885AspIle: 1.885 ± 0.085
1.496AspLys: 1.496 ± 0.078
5.541AspLeu: 5.541 ± 0.119
0.733AspMet: 0.733 ± 0.052
1.28AspAsn: 1.28 ± 0.067
3.271AspPro: 3.271 ± 0.128
1.675AspGln: 1.675 ± 0.066
3.66AspArg: 3.66 ± 0.107
2.504AspSer: 2.504 ± 0.092
2.263AspThr: 2.263 ± 0.073
3.563AspVal: 3.563 ± 0.124
0.87AspTrp: 0.87 ± 0.045
1.531AspTyr: 1.531 ± 0.075
0.0AspXaa: 0.0 ± 0.0
Glu
5.939GluAla: 5.939 ± 0.163
0.388GluCys: 0.388 ± 0.035
2.163GluAsp: 2.163 ± 0.094
2.761GluGlu: 2.761 ± 0.111
2.417GluPhe: 2.417 ± 0.096
3.531GluGly: 3.531 ± 0.126
1.039GluHis: 1.039 ± 0.055
3.509GluIle: 3.509 ± 0.11
2.492GluLys: 2.492 ± 0.096
5.56GluLeu: 5.56 ± 0.149
1.506GluMet: 1.506 ± 0.066
1.731GluAsn: 1.731 ± 0.078
2.648GluPro: 2.648 ± 0.08
2.057GluGln: 2.057 ± 0.086
4.586GluArg: 4.586 ± 0.144
3.024GluSer: 3.024 ± 0.093
2.833GluThr: 2.833 ± 0.1
4.148GluVal: 4.148 ± 0.133
0.764GluTrp: 0.764 ± 0.051
1.365GluTyr: 1.365 ± 0.075
0.0GluXaa: 0.0 ± 0.0
Phe
5.15PheAla: 5.15 ± 0.15
0.454PheCys: 0.454 ± 0.037
2.645PheAsp: 2.645 ± 0.117
2.254PheGlu: 2.254 ± 0.087
1.963PhePhe: 1.963 ± 0.088
4.067PheGly: 4.067 ± 0.111
0.867PheHis: 0.867 ± 0.054
1.766PheIle: 1.766 ± 0.079
1.293PheLys: 1.293 ± 0.074
4.186PheLeu: 4.186 ± 0.142
0.729PheMet: 0.729 ± 0.05
1.349PheAsn: 1.349 ± 0.079
1.906PhePro: 1.906 ± 0.079
1.368PheGln: 1.368 ± 0.074
2.758PheArg: 2.758 ± 0.097
2.839PheSer: 2.839 ± 0.102
2.464PheThr: 2.464 ± 0.097
3.203PheVal: 3.203 ± 0.113
0.67PheTrp: 0.67 ± 0.047
1.158PheTyr: 1.158 ± 0.064
0.0PheXaa: 0.0 ± 0.0
Gly
8.675GlyAla: 8.675 ± 0.229
0.88GlyCys: 0.88 ± 0.051
3.919GlyAsp: 3.919 ± 0.124
4.17GlyGlu: 4.17 ± 0.115
3.81GlyPhe: 3.81 ± 0.129
7.466GlyGly: 7.466 ± 0.21
1.678GlyHis: 1.678 ± 0.074
3.944GlyIle: 3.944 ± 0.139
3.275GlyLys: 3.275 ± 0.136
8.29GlyLeu: 8.29 ± 0.198
1.888GlyMet: 1.888 ± 0.081
2.504GlyAsn: 2.504 ± 0.137
3.556GlyPro: 3.556 ± 0.116
2.62GlyGln: 2.62 ± 0.096
5.704GlyArg: 5.704 ± 0.137
4.884GlySer: 4.884 ± 0.151
4.912GlyThr: 4.912 ± 0.191
6.414GlyVal: 6.414 ± 0.135
1.409GlyTrp: 1.409 ± 0.073
1.925GlyTyr: 1.925 ± 0.091
0.0GlyXaa: 0.0 ± 0.0
His
2.148HisAla: 2.148 ± 0.085
0.219HisCys: 0.219 ± 0.031
0.977HisAsp: 0.977 ± 0.059
1.193HisGlu: 1.193 ± 0.062
1.058HisPhe: 1.058 ± 0.072
1.841HisGly: 1.841 ± 0.093
0.542HisHis: 0.542 ± 0.045
0.798HisIle: 0.798 ± 0.051
0.448HisLys: 0.448 ± 0.04
2.113HisLeu: 2.113 ± 0.083
0.304HisMet: 0.304 ± 0.03
0.438HisAsn: 0.438 ± 0.038
1.409HisPro: 1.409 ± 0.069
0.598HisGln: 0.598 ± 0.05
1.681HisArg: 1.681 ± 0.079
1.033HisSer: 1.033 ± 0.062
0.88HisThr: 0.88 ± 0.07
1.318HisVal: 1.318 ± 0.065
0.332HisTrp: 0.332 ± 0.035
0.614HisTyr: 0.614 ± 0.045
0.0HisXaa: 0.0 ± 0.0
Ile
6.073IleAla: 6.073 ± 0.154
0.589IleCys: 0.589 ± 0.043
2.864IleAsp: 2.864 ± 0.094
3.134IleGlu: 3.134 ± 0.119
2.194IlePhe: 2.194 ± 0.084
4.013IleGly: 4.013 ± 0.134
1.143IleHis: 1.143 ± 0.065
2.245IleIle: 2.245 ± 0.098
1.525IleLys: 1.525 ± 0.078
4.486IleLeu: 4.486 ± 0.116
0.717IleMet: 0.717 ± 0.052
1.468IleAsn: 1.468 ± 0.069
2.533IlePro: 2.533 ± 0.096
1.412IleGln: 1.412 ± 0.074
3.268IleArg: 3.268 ± 0.102
3.033IleSer: 3.033 ± 0.117
2.601IleThr: 2.601 ± 0.119
4.104IleVal: 4.104 ± 0.114
0.57IleTrp: 0.57 ± 0.049
1.343IleTyr: 1.343 ± 0.076
0.0IleXaa: 0.0 ± 0.0
Lys
3.644LysAla: 3.644 ± 0.129
0.232LysCys: 0.232 ± 0.025
1.65LysAsp: 1.65 ± 0.093
1.844LysGlu: 1.844 ± 0.097
1.553LysPhe: 1.553 ± 0.069
2.241LysGly: 2.241 ± 0.098
0.667LysHis: 0.667 ± 0.05
2.101LysIle: 2.101 ± 0.092
1.791LysLys: 1.791 ± 0.105
3.65LysLeu: 3.65 ± 0.121
0.983LysMet: 0.983 ± 0.055
1.077LysAsn: 1.077 ± 0.055
2.273LysPro: 2.273 ± 0.105
1.196LysGln: 1.196 ± 0.058
2.451LysArg: 2.451 ± 0.094
2.279LysSer: 2.279 ± 0.096
2.163LysThr: 2.163 ± 0.098
2.467LysVal: 2.467 ± 0.095
0.47LysTrp: 0.47 ± 0.04
0.855LysTyr: 0.855 ± 0.058
0.0LysXaa: 0.0 ± 0.0
Leu
13.483LeuAla: 13.483 ± 0.311
0.911LeuCys: 0.911 ± 0.056
5.328LeuAsp: 5.328 ± 0.121
5.363LeuGlu: 5.363 ± 0.15
3.894LeuPhe: 3.894 ± 0.148
8.503LeuGly: 8.503 ± 0.181
2.126LeuHis: 2.126 ± 0.083
4.655LeuIle: 4.655 ± 0.125
3.628LeuLys: 3.628 ± 0.119
10.434LeuLeu: 10.434 ± 0.279
1.825LeuMet: 1.825 ± 0.084
2.902LeuAsn: 2.902 ± 0.107
6.111LeuPro: 6.111 ± 0.159
3.315LeuGln: 3.315 ± 0.107
7.685LeuArg: 7.685 ± 0.185
5.813LeuSer: 5.813 ± 0.119
5.385LeuThr: 5.385 ± 0.14
8.086LeuVal: 8.086 ± 0.181
1.356LeuTrp: 1.356 ± 0.076
2.119LeuTyr: 2.119 ± 0.081
0.0LeuXaa: 0.0 ± 0.0
Met
2.21MetAla: 2.21 ± 0.089
0.144MetCys: 0.144 ± 0.023
0.883MetAsp: 0.883 ± 0.047
1.042MetGlu: 1.042 ± 0.058
0.676MetPhe: 0.676 ± 0.049
1.496MetGly: 1.496 ± 0.081
0.429MetHis: 0.429 ± 0.038
1.183MetIle: 1.183 ± 0.068
1.193MetLys: 1.193 ± 0.065
2.129MetLeu: 2.129 ± 0.087
0.473MetMet: 0.473 ± 0.047
0.792MetAsn: 0.792 ± 0.054
1.271MetPro: 1.271 ± 0.065
0.745MetGln: 0.745 ± 0.046
1.546MetArg: 1.546 ± 0.068
1.424MetSer: 1.424 ± 0.066
1.377MetThr: 1.377 ± 0.061
1.271MetVal: 1.271 ± 0.066
0.222MetTrp: 0.222 ± 0.028
0.301MetTyr: 0.301 ± 0.033
0.0MetXaa: 0.0 ± 0.0
Asn
3.237AsnAla: 3.237 ± 0.098
0.307AsnCys: 0.307 ± 0.038
1.453AsnAsp: 1.453 ± 0.065
1.421AsnGlu: 1.421 ± 0.073
1.434AsnPhe: 1.434 ± 0.083
2.614AsnGly: 2.614 ± 0.139
0.589AsnHis: 0.589 ± 0.045
1.412AsnIle: 1.412 ± 0.075
0.845AsnLys: 0.845 ± 0.054
3.218AsnLeu: 3.218 ± 0.101
0.513AsnMet: 0.513 ± 0.044
0.924AsnAsn: 0.924 ± 0.072
2.094AsnPro: 2.094 ± 0.09
0.93AsnGln: 0.93 ± 0.069
1.885AsnArg: 1.885 ± 0.079
1.64AsnSer: 1.64 ± 0.071
1.553AsnThr: 1.553 ± 0.077
2.238AsnVal: 2.238 ± 0.092
0.448AsnTrp: 0.448 ± 0.041
0.958AsnTyr: 0.958 ± 0.058
0.0AsnXaa: 0.0 ± 0.0
Pro
7.235ProAla: 7.235 ± 0.221
0.313ProCys: 0.313 ± 0.029
3.368ProAsp: 3.368 ± 0.117
3.656ProGlu: 3.656 ± 0.122
2.216ProPhe: 2.216 ± 0.083
4.956ProGly: 4.956 ± 0.153
0.992ProHis: 0.992 ± 0.055
2.576ProIle: 2.576 ± 0.107
1.91ProLys: 1.91 ± 0.085
5.09ProLeu: 5.09 ± 0.133
1.212ProMet: 1.212 ± 0.063
1.831ProAsn: 1.831 ± 0.081
3.857ProPro: 3.857 ± 0.226
1.618ProGln: 1.618 ± 0.072
3.253ProArg: 3.253 ± 0.133
2.94ProSer: 2.94 ± 0.129
3.287ProThr: 3.287 ± 0.129
4.392ProVal: 4.392 ± 0.128
0.751ProTrp: 0.751 ± 0.048
1.174ProTyr: 1.174 ± 0.068
0.0ProXaa: 0.0 ± 0.0
Gln
3.666GlnAla: 3.666 ± 0.095
0.254GlnCys: 0.254 ± 0.032
1.327GlnAsp: 1.327 ± 0.075
1.459GlnGlu: 1.459 ± 0.059
1.456GlnPhe: 1.456 ± 0.078
1.972GlnGly: 1.972 ± 0.084
0.745GlnHis: 0.745 ± 0.059
1.888GlnIle: 1.888 ± 0.081
1.171GlnLys: 1.171 ± 0.06
3.4GlnLeu: 3.4 ± 0.123
0.764GlnMet: 0.764 ± 0.045
0.992GlnAsn: 0.992 ± 0.057
2.097GlnPro: 2.097 ± 0.086
1.293GlnGln: 1.293 ± 0.081
2.714GlnArg: 2.714 ± 0.104
1.875GlnSer: 1.875 ± 0.077
1.853GlnThr: 1.853 ± 0.08
2.395GlnVal: 2.395 ± 0.096
0.466GlnTrp: 0.466 ± 0.039
0.751GlnTyr: 0.751 ± 0.056
0.0GlnXaa: 0.0 ± 0.0
Arg
7.964ArgAla: 7.964 ± 0.192
0.639ArgCys: 0.639 ± 0.053
3.804ArgAsp: 3.804 ± 0.119
4.505ArgGlu: 4.505 ± 0.136
3.262ArgPhe: 3.262 ± 0.108
4.984ArgGly: 4.984 ± 0.126
1.506ArgHis: 1.506 ± 0.086
3.813ArgIle: 3.813 ± 0.112
2.498ArgLys: 2.498 ± 0.099
7.548ArgLeu: 7.548 ± 0.205
1.597ArgMet: 1.597 ± 0.081
1.819ArgAsn: 1.819 ± 0.069
3.588ArgPro: 3.588 ± 0.124
2.429ArgGln: 2.429 ± 0.1
5.845ArgArg: 5.845 ± 0.186
3.556ArgSer: 3.556 ± 0.102
3.437ArgThr: 3.437 ± 0.106
5.35ArgVal: 5.35 ± 0.134
1.215ArgTrp: 1.215 ± 0.059
1.888ArgTyr: 1.888 ± 0.08
0.0ArgXaa: 0.0 ± 0.0
Ser
6.371SerAla: 6.371 ± 0.17
0.391SerCys: 0.391 ± 0.033
3.015SerAsp: 3.015 ± 0.108
2.68SerGlu: 2.68 ± 0.089
2.398SerPhe: 2.398 ± 0.089
5.435SerGly: 5.435 ± 0.158
1.171SerHis: 1.171 ± 0.062
2.636SerIle: 2.636 ± 0.095
1.834SerLys: 1.834 ± 0.086
5.923SerLeu: 5.923 ± 0.164
1.202SerMet: 1.202 ± 0.074
1.7SerAsn: 1.7 ± 0.088
3.641SerPro: 3.641 ± 0.124
1.528SerGln: 1.528 ± 0.074
3.628SerArg: 3.628 ± 0.117
3.256SerSer: 3.256 ± 0.126
3.071SerThr: 3.071 ± 0.112
4.073SerVal: 4.073 ± 0.115
0.795SerTrp: 0.795 ± 0.05
1.478SerTyr: 1.478 ± 0.089
0.0SerXaa: 0.0 ± 0.0
Thr
5.973ThrAla: 5.973 ± 0.17
0.36ThrCys: 0.36 ± 0.035
2.548ThrAsp: 2.548 ± 0.097
2.473ThrGlu: 2.473 ± 0.086
2.232ThrPhe: 2.232 ± 0.094
5.115ThrGly: 5.115 ± 0.189
0.949ThrHis: 0.949 ± 0.058
3.187ThrIle: 3.187 ± 0.109
1.91ThrLys: 1.91 ± 0.092
5.601ThrLeu: 5.601 ± 0.158
1.099ThrMet: 1.099 ± 0.055
1.665ThrAsn: 1.665 ± 0.099
3.719ThrPro: 3.719 ± 0.137
1.637ThrGln: 1.637 ± 0.069
3.246ThrArg: 3.246 ± 0.116
3.209ThrSer: 3.209 ± 0.118
3.199ThrThr: 3.199 ± 0.147
4.17ThrVal: 4.17 ± 0.127
0.801ThrTrp: 0.801 ± 0.06
1.603ThrTyr: 1.603 ± 0.093
0.0ThrXaa: 0.0 ± 0.0
Val
9.035ValAla: 9.035 ± 0.231
0.704ValCys: 0.704 ± 0.048
3.7ValAsp: 3.7 ± 0.108
4.395ValGlu: 4.395 ± 0.121
3.275ValPhe: 3.275 ± 0.1
5.554ValGly: 5.554 ± 0.146
1.374ValHis: 1.374 ± 0.067
3.854ValIle: 3.854 ± 0.107
2.385ValLys: 2.385 ± 0.091
7.494ValLeu: 7.494 ± 0.192
1.5ValMet: 1.5 ± 0.059
2.339ValAsn: 2.339 ± 0.087
4.405ValPro: 4.405 ± 0.126
2.326ValGln: 2.326 ± 0.089
5.256ValArg: 5.256 ± 0.137
4.179ValSer: 4.179 ± 0.13
4.589ValThr: 4.589 ± 0.151
6.283ValVal: 6.283 ± 0.168
1.005ValTrp: 1.005 ± 0.07
1.534ValTyr: 1.534 ± 0.068
0.0ValXaa: 0.0 ± 0.0
Trp
1.14TrpAla: 1.14 ± 0.071
0.138TrpCys: 0.138 ± 0.021
0.717TrpAsp: 0.717 ± 0.06
0.704TrpGlu: 0.704 ± 0.047
0.614TrpPhe: 0.614 ± 0.046
0.945TrpGly: 0.945 ± 0.057
0.288TrpHis: 0.288 ± 0.028
0.858TrpIle: 0.858 ± 0.056
0.567TrpLys: 0.567 ± 0.037
1.928TrpLeu: 1.928 ± 0.085
0.351TrpMet: 0.351 ± 0.035
0.538TrpAsn: 0.538 ± 0.045
0.72TrpPro: 0.72 ± 0.043
0.598TrpGln: 0.598 ± 0.049
1.271TrpArg: 1.271 ± 0.068
0.933TrpSer: 0.933 ± 0.056
0.867TrpThr: 0.867 ± 0.063
0.917TrpVal: 0.917 ± 0.059
0.297TrpTrp: 0.297 ± 0.031
0.347TrpTyr: 0.347 ± 0.036
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.667TyrAla: 2.667 ± 0.093
0.232TyrCys: 0.232 ± 0.028
1.402TyrAsp: 1.402 ± 0.09
1.324TyrGlu: 1.324 ± 0.071
1.268TyrPhe: 1.268 ± 0.067
2.141TyrGly: 2.141 ± 0.107
0.598TyrHis: 0.598 ± 0.044
0.895TyrIle: 0.895 ± 0.059
0.648TyrLys: 0.648 ± 0.052
2.595TyrLeu: 2.595 ± 0.083
0.391TyrMet: 0.391 ± 0.038
0.82TyrAsn: 0.82 ± 0.063
1.312TyrPro: 1.312 ± 0.066
0.952TyrGln: 0.952 ± 0.066
2.004TyrArg: 2.004 ± 0.08
1.274TyrSer: 1.274 ± 0.067
1.208TyrThr: 1.208 ± 0.08
1.703TyrVal: 1.703 ± 0.071
0.46TyrTrp: 0.46 ± 0.036
0.82TyrTyr: 0.82 ± 0.063
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1004 proteins (319436 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski