Amino acid dipepetide frequency for Bacillus phage Belinda

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.289AlaAla: 4.289 ± 0.683
0.425AlaCys: 0.425 ± 0.077
3.439AlaAsp: 3.439 ± 0.341
4.612AlaGlu: 4.612 ± 0.288
2.65AlaPhe: 2.65 ± 0.225
3.48AlaGly: 3.48 ± 0.433
1.113AlaHis: 1.113 ± 0.137
4.329AlaIle: 4.329 ± 0.293
4.977AlaLys: 4.977 ± 0.297
5.037AlaLeu: 5.037 ± 0.285
1.983AlaMet: 1.983 ± 0.193
3.439AlaAsn: 3.439 ± 0.346
1.72AlaPro: 1.72 ± 0.209
2.508AlaGln: 2.508 ± 0.23
2.61AlaArg: 2.61 ± 0.231
3.176AlaSer: 3.176 ± 0.347
3.925AlaThr: 3.925 ± 0.403
4.046AlaVal: 4.046 ± 0.301
0.708AlaTrp: 0.708 ± 0.127
2.589AlaTyr: 2.589 ± 0.223
0.0AlaXaa: 0.0 ± 0.0
Cys
0.324CysAla: 0.324 ± 0.074
0.101CysCys: 0.101 ± 0.049
0.607CysAsp: 0.607 ± 0.111
0.89CysGlu: 0.89 ± 0.153
0.465CysPhe: 0.465 ± 0.101
0.688CysGly: 0.688 ± 0.119
0.263CysHis: 0.263 ± 0.074
0.546CysIle: 0.546 ± 0.103
0.749CysLys: 0.749 ± 0.134
0.546CysLeu: 0.546 ± 0.098
0.243CysMet: 0.243 ± 0.071
0.445CysAsn: 0.445 ± 0.098
0.324CysPro: 0.324 ± 0.093
0.283CysGln: 0.283 ± 0.077
0.384CysArg: 0.384 ± 0.087
0.465CysSer: 0.465 ± 0.095
0.688CysThr: 0.688 ± 0.117
0.506CysVal: 0.506 ± 0.099
0.142CysTrp: 0.142 ± 0.057
0.384CysTyr: 0.384 ± 0.102
0.0CysXaa: 0.0 ± 0.0
Asp
4.329AspAla: 4.329 ± 0.307
0.647AspCys: 0.647 ± 0.105
4.046AspAsp: 4.046 ± 0.29
5.563AspGlu: 5.563 ± 0.382
2.954AspPhe: 2.954 ± 0.265
3.965AspGly: 3.965 ± 0.296
0.708AspHis: 0.708 ± 0.145
5.26AspIle: 5.26 ± 0.352
5.948AspLys: 5.948 ± 0.368
5.037AspLeu: 5.037 ± 0.35
2.367AspMet: 2.367 ± 0.231
3.277AspAsn: 3.277 ± 0.258
1.639AspPro: 1.639 ± 0.206
1.052AspGln: 1.052 ± 0.131
2.832AspArg: 2.832 ± 0.208
3.338AspSer: 3.338 ± 0.241
3.884AspThr: 3.884 ± 0.374
4.997AspVal: 4.997 ± 0.289
0.85AspTrp: 0.85 ± 0.16
3.217AspTyr: 3.217 ± 0.269
0.0AspXaa: 0.0 ± 0.0
Glu
4.41GluAla: 4.41 ± 0.374
0.809GluCys: 0.809 ± 0.147
4.896GluAsp: 4.896 ± 0.397
9.205GluGlu: 9.205 ± 0.805
3.52GluPhe: 3.52 ± 0.267
4.977GluGly: 4.977 ± 0.298
1.578GluHis: 1.578 ± 0.205
5.341GluIle: 5.341 ± 0.348
5.786GluLys: 5.786 ± 0.478
8.213GluLeu: 8.213 ± 0.495
3.095GluMet: 3.095 ± 0.217
3.54GluAsn: 3.54 ± 0.226
1.942GluPro: 1.942 ± 0.213
3.055GluGln: 3.055 ± 0.245
3.419GluArg: 3.419 ± 0.305
4.127GluSer: 4.127 ± 0.253
3.722GluThr: 3.722 ± 0.274
6.332GluVal: 6.332 ± 0.509
1.011GluTrp: 1.011 ± 0.169
3.156GluTyr: 3.156 ± 0.271
0.0GluXaa: 0.0 ± 0.0
Phe
2.286PheAla: 2.286 ± 0.183
0.445PheCys: 0.445 ± 0.087
3.277PheAsp: 3.277 ± 0.288
2.508PheGlu: 2.508 ± 0.21
1.497PhePhe: 1.497 ± 0.194
2.124PheGly: 2.124 ± 0.198
0.809PheHis: 0.809 ± 0.122
2.873PheIle: 2.873 ± 0.226
2.994PheLys: 2.994 ± 0.288
2.893PheLeu: 2.893 ± 0.25
1.315PheMet: 1.315 ± 0.155
2.893PheAsn: 2.893 ± 0.258
1.092PhePro: 1.092 ± 0.159
1.234PheGln: 1.234 ± 0.135
1.618PheArg: 1.618 ± 0.231
2.104PheSer: 2.104 ± 0.191
3.358PheThr: 3.358 ± 0.265
2.852PheVal: 2.852 ± 0.243
0.364PheTrp: 0.364 ± 0.097
1.76PheTyr: 1.76 ± 0.191
0.0PheXaa: 0.0 ± 0.0
Gly
3.459GlyAla: 3.459 ± 0.641
0.607GlyCys: 0.607 ± 0.115
3.257GlyAsp: 3.257 ± 0.234
4.673GlyGlu: 4.673 ± 0.326
2.852GlyPhe: 2.852 ± 0.218
4.875GlyGly: 4.875 ± 0.808
0.749GlyHis: 0.749 ± 0.133
3.864GlyIle: 3.864 ± 0.383
4.916GlyLys: 4.916 ± 0.335
4.046GlyLeu: 4.046 ± 0.329
1.841GlyMet: 1.841 ± 0.216
2.974GlyAsn: 2.974 ± 0.31
0.668GlyPro: 0.668 ± 0.105
1.841GlyGln: 1.841 ± 0.257
2.913GlyArg: 2.913 ± 0.203
3.338GlySer: 3.338 ± 0.301
4.188GlyThr: 4.188 ± 0.354
5.482GlyVal: 5.482 ± 0.33
1.092GlyTrp: 1.092 ± 0.147
3.358GlyTyr: 3.358 ± 0.266
0.0GlyXaa: 0.0 ± 0.0
His
0.89HisAla: 0.89 ± 0.151
0.182HisCys: 0.182 ± 0.059
1.113HisAsp: 1.113 ± 0.168
1.315HisGlu: 1.315 ± 0.162
0.87HisPhe: 0.87 ± 0.153
1.113HisGly: 1.113 ± 0.148
0.243HisHis: 0.243 ± 0.062
1.315HisIle: 1.315 ± 0.168
1.477HisLys: 1.477 ± 0.21
1.335HisLeu: 1.335 ± 0.168
0.627HisMet: 0.627 ± 0.105
1.133HisAsn: 1.133 ± 0.173
0.647HisPro: 0.647 ± 0.119
0.708HisGln: 0.708 ± 0.128
0.708HisArg: 0.708 ± 0.12
0.87HisSer: 0.87 ± 0.14
1.153HisThr: 1.153 ± 0.162
1.679HisVal: 1.679 ± 0.217
0.223HisTrp: 0.223 ± 0.068
0.951HisTyr: 0.951 ± 0.136
0.0HisXaa: 0.0 ± 0.0
Ile
4.208IleAla: 4.208 ± 0.303
0.769IleCys: 0.769 ± 0.154
5.199IleAsp: 5.199 ± 0.364
5.523IleGlu: 5.523 ± 0.31
1.578IlePhe: 1.578 ± 0.169
3.803IleGly: 3.803 ± 0.302
1.072IleHis: 1.072 ± 0.168
4.066IleIle: 4.066 ± 0.322
5.846IleLys: 5.846 ± 0.337
4.167IleLeu: 4.167 ± 0.286
1.861IleMet: 1.861 ± 0.173
3.783IleAsn: 3.783 ± 0.318
2.084IlePro: 2.084 ± 0.251
2.286IleGln: 2.286 ± 0.235
3.196IleArg: 3.196 ± 0.217
4.127IleSer: 4.127 ± 0.271
4.754IleThr: 4.754 ± 0.341
4.471IleVal: 4.471 ± 0.378
0.647IleTrp: 0.647 ± 0.108
2.165IleTyr: 2.165 ± 0.204
0.0IleXaa: 0.0 ± 0.0
Lys
4.997LysAla: 4.997 ± 0.405
0.647LysCys: 0.647 ± 0.116
5.401LysAsp: 5.401 ± 0.308
7.809LysGlu: 7.809 ± 0.575
2.468LysPhe: 2.468 ± 0.266
5.017LysGly: 5.017 ± 0.322
1.861LysHis: 1.861 ± 0.208
4.066LysIle: 4.066 ± 0.278
5.583LysLys: 5.583 ± 0.42
6.595LysLeu: 6.595 ± 0.383
2.731LysMet: 2.731 ± 0.222
4.107LysAsn: 4.107 ± 0.329
2.326LysPro: 2.326 ± 0.226
3.358LysGln: 3.358 ± 0.265
3.358LysArg: 3.358 ± 0.287
3.884LysSer: 3.884 ± 0.321
4.329LysThr: 4.329 ± 0.307
5.745LysVal: 5.745 ± 0.315
0.991LysTrp: 0.991 ± 0.128
3.055LysTyr: 3.055 ± 0.271
0.0LysXaa: 0.0 ± 0.0
Leu
4.956LeuAla: 4.956 ± 0.283
0.627LeuCys: 0.627 ± 0.115
5.786LeuAsp: 5.786 ± 0.349
7.364LeuGlu: 7.364 ± 0.53
2.67LeuPhe: 2.67 ± 0.237
4.835LeuGly: 4.835 ± 0.361
1.173LeuHis: 1.173 ± 0.147
4.835LeuIle: 4.835 ± 0.317
5.563LeuLys: 5.563 ± 0.326
5.705LeuLeu: 5.705 ± 0.378
2.367LeuMet: 2.367 ± 0.217
4.309LeuAsn: 4.309 ± 0.267
2.65LeuPro: 2.65 ± 0.242
2.832LeuGln: 2.832 ± 0.246
4.026LeuArg: 4.026 ± 0.315
4.835LeuSer: 4.835 ± 0.291
5.199LeuThr: 5.199 ± 0.322
5.442LeuVal: 5.442 ± 0.421
0.829LeuTrp: 0.829 ± 0.132
2.812LeuTyr: 2.812 ± 0.3
0.0LeuXaa: 0.0 ± 0.0
Met
1.679MetAla: 1.679 ± 0.168
0.364MetCys: 0.364 ± 0.105
2.023MetAsp: 2.023 ± 0.21
2.084MetGlu: 2.084 ± 0.193
1.477MetPhe: 1.477 ± 0.169
1.8MetGly: 1.8 ± 0.235
0.647MetHis: 0.647 ± 0.133
1.74MetIle: 1.74 ± 0.193
2.873MetLys: 2.873 ± 0.292
2.63MetLeu: 2.63 ± 0.21
0.708MetMet: 0.708 ± 0.144
1.902MetAsn: 1.902 ± 0.207
0.789MetPro: 0.789 ± 0.135
0.789MetGln: 0.789 ± 0.126
1.578MetArg: 1.578 ± 0.188
2.326MetSer: 2.326 ± 0.181
2.468MetThr: 2.468 ± 0.237
1.679MetVal: 1.679 ± 0.198
0.364MetTrp: 0.364 ± 0.086
1.598MetTyr: 1.598 ± 0.172
0.0MetXaa: 0.0 ± 0.0
Asn
3.237AsnAla: 3.237 ± 0.302
0.405AsnCys: 0.405 ± 0.1
3.136AsnAsp: 3.136 ± 0.252
3.5AsnGlu: 3.5 ± 0.295
1.983AsnPhe: 1.983 ± 0.171
4.329AsnGly: 4.329 ± 0.347
1.113AsnHis: 1.113 ± 0.14
3.722AsnIle: 3.722 ± 0.278
4.673AsnLys: 4.673 ± 0.313
3.641AsnLeu: 3.641 ± 0.27
1.861AsnMet: 1.861 ± 0.181
3.459AsnAsn: 3.459 ± 0.309
2.023AsnPro: 2.023 ± 0.262
1.76AsnGln: 1.76 ± 0.192
2.792AsnArg: 2.792 ± 0.198
2.792AsnSer: 2.792 ± 0.226
3.217AsnThr: 3.217 ± 0.326
3.56AsnVal: 3.56 ± 0.256
0.708AsnTrp: 0.708 ± 0.111
2.387AsnTyr: 2.387 ± 0.201
0.0AsnXaa: 0.0 ± 0.0
Pro
1.8ProAla: 1.8 ± 0.263
0.243ProCys: 0.243 ± 0.085
2.266ProAsp: 2.266 ± 0.229
2.63ProGlu: 2.63 ± 0.241
1.477ProPhe: 1.477 ± 0.192
0.02ProGly: 0.02 ± 0.019
0.566ProHis: 0.566 ± 0.116
1.497ProIle: 1.497 ± 0.208
2.124ProLys: 2.124 ± 0.216
2.104ProLeu: 2.104 ± 0.202
0.708ProMet: 0.708 ± 0.137
1.76ProAsn: 1.76 ± 0.221
0.728ProPro: 0.728 ± 0.153
0.789ProGln: 0.789 ± 0.138
1.234ProArg: 1.234 ± 0.155
1.962ProSer: 1.962 ± 0.225
2.407ProThr: 2.407 ± 0.305
1.922ProVal: 1.922 ± 0.246
0.223ProTrp: 0.223 ± 0.064
1.416ProTyr: 1.416 ± 0.169
0.0ProXaa: 0.0 ± 0.0
Gln
2.326GlnAla: 2.326 ± 0.306
0.283GlnCys: 0.283 ± 0.078
2.084GlnAsp: 2.084 ± 0.21
2.468GlnGlu: 2.468 ± 0.185
1.396GlnPhe: 1.396 ± 0.16
1.78GlnGly: 1.78 ± 0.215
0.749GlnHis: 0.749 ± 0.122
1.841GlnIle: 1.841 ± 0.201
2.003GlnLys: 2.003 ± 0.263
3.318GlnLeu: 3.318 ± 0.283
1.072GlnMet: 1.072 ± 0.177
1.436GlnAsn: 1.436 ± 0.177
0.951GlnPro: 0.951 ± 0.146
1.274GlnGln: 1.274 ± 0.246
1.618GlnArg: 1.618 ± 0.192
1.881GlnSer: 1.881 ± 0.199
1.962GlnThr: 1.962 ± 0.227
2.165GlnVal: 2.165 ± 0.204
0.324GlnTrp: 0.324 ± 0.08
1.517GlnTyr: 1.517 ± 0.156
0.0GlnXaa: 0.0 ± 0.0
Arg
2.873ArgAla: 2.873 ± 0.249
0.324ArgCys: 0.324 ± 0.083
2.852ArgAsp: 2.852 ± 0.218
3.702ArgGlu: 3.702 ± 0.348
1.983ArgPhe: 1.983 ± 0.176
2.65ArgGly: 2.65 ± 0.269
0.931ArgHis: 0.931 ± 0.15
3.014ArgIle: 3.014 ± 0.244
3.399ArgLys: 3.399 ± 0.282
3.682ArgLeu: 3.682 ± 0.318
1.639ArgMet: 1.639 ± 0.212
2.771ArgAsn: 2.771 ± 0.272
1.214ArgPro: 1.214 ± 0.144
1.517ArgGln: 1.517 ± 0.183
1.74ArgArg: 1.74 ± 0.204
1.983ArgSer: 1.983 ± 0.233
2.387ArgThr: 2.387 ± 0.202
3.601ArgVal: 3.601 ± 0.271
0.546ArgTrp: 0.546 ± 0.097
2.104ArgTyr: 2.104 ± 0.19
0.0ArgXaa: 0.0 ± 0.0
Ser
3.743SerAla: 3.743 ± 0.303
0.344SerCys: 0.344 ± 0.091
3.297SerAsp: 3.297 ± 0.208
3.54SerGlu: 3.54 ± 0.272
2.751SerPhe: 2.751 ± 0.256
3.904SerGly: 3.904 ± 0.347
0.991SerHis: 0.991 ± 0.152
4.107SerIle: 4.107 ± 0.318
3.965SerLys: 3.965 ± 0.325
4.633SerLeu: 4.633 ± 0.263
1.821SerMet: 1.821 ± 0.204
2.812SerAsn: 2.812 ± 0.239
1.457SerPro: 1.457 ± 0.21
1.8SerGln: 1.8 ± 0.203
2.65SerArg: 2.65 ± 0.228
3.318SerSer: 3.318 ± 0.355
3.358SerThr: 3.358 ± 0.285
3.5SerVal: 3.5 ± 0.253
0.627SerTrp: 0.627 ± 0.103
2.751SerTyr: 2.751 ± 0.243
0.0SerXaa: 0.0 ± 0.0
Thr
4.39ThrAla: 4.39 ± 0.459
0.425ThrCys: 0.425 ± 0.113
4.086ThrAsp: 4.086 ± 0.357
4.39ThrGlu: 4.39 ± 0.289
3.257ThrPhe: 3.257 ± 0.3
4.491ThrGly: 4.491 ± 0.383
1.396ThrHis: 1.396 ± 0.163
4.531ThrIle: 4.531 ± 0.274
4.673ThrLys: 4.673 ± 0.26
4.977ThrLeu: 4.977 ± 0.299
1.78ThrMet: 1.78 ± 0.173
3.277ThrAsn: 3.277 ± 0.262
2.448ThrPro: 2.448 ± 0.25
1.517ThrGln: 1.517 ± 0.251
2.893ThrArg: 2.893 ± 0.25
3.338ThrSer: 3.338 ± 0.296
4.107ThrThr: 4.107 ± 0.401
5.017ThrVal: 5.017 ± 0.361
0.607ThrTrp: 0.607 ± 0.109
2.792ThrTyr: 2.792 ± 0.236
0.0ThrXaa: 0.0 ± 0.0
Val
4.006ValAla: 4.006 ± 0.275
0.85ValCys: 0.85 ± 0.15
4.916ValAsp: 4.916 ± 0.31
5.664ValGlu: 5.664 ± 0.468
2.589ValPhe: 2.589 ± 0.258
4.046ValGly: 4.046 ± 0.332
1.457ValHis: 1.457 ± 0.193
4.754ValIle: 4.754 ± 0.289
6.13ValLys: 6.13 ± 0.386
5.988ValLeu: 5.988 ± 0.39
1.902ValMet: 1.902 ± 0.208
3.904ValAsn: 3.904 ± 0.305
1.983ValPro: 1.983 ± 0.262
2.124ValGln: 2.124 ± 0.213
2.852ValArg: 2.852 ± 0.233
4.046ValSer: 4.046 ± 0.266
5.482ValThr: 5.482 ± 0.381
5.26ValVal: 5.26 ± 0.41
0.587ValTrp: 0.587 ± 0.12
3.803ValTyr: 3.803 ± 0.277
0.0ValXaa: 0.0 ± 0.0
Trp
0.647TrpAla: 0.647 ± 0.106
0.081TrpCys: 0.081 ± 0.04
1.052TrpAsp: 1.052 ± 0.143
1.113TrpGlu: 1.113 ± 0.166
0.445TrpPhe: 0.445 ± 0.099
0.708TrpGly: 0.708 ± 0.125
0.243TrpHis: 0.243 ± 0.067
0.809TrpIle: 0.809 ± 0.12
0.749TrpLys: 0.749 ± 0.12
0.769TrpLeu: 0.769 ± 0.115
0.344TrpMet: 0.344 ± 0.081
0.566TrpAsn: 0.566 ± 0.133
0.0TrpPro: 0.0 ± 0.0
0.425TrpGln: 0.425 ± 0.102
0.324TrpArg: 0.324 ± 0.074
0.749TrpSer: 0.749 ± 0.132
0.829TrpThr: 0.829 ± 0.189
0.87TrpVal: 0.87 ± 0.129
0.121TrpTrp: 0.121 ± 0.05
0.708TrpTyr: 0.708 ± 0.118
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.205TyrAla: 2.205 ± 0.252
0.465TyrCys: 0.465 ± 0.097
3.176TyrAsp: 3.176 ± 0.281
3.581TyrGlu: 3.581 ± 0.315
1.416TyrPhe: 1.416 ± 0.18
2.387TyrGly: 2.387 ± 0.257
0.89TyrHis: 0.89 ± 0.147
3.095TyrIle: 3.095 ± 0.261
3.904TyrLys: 3.904 ± 0.302
3.5TyrLeu: 3.5 ± 0.268
1.234TyrMet: 1.234 ± 0.158
2.508TyrAsn: 2.508 ± 0.22
1.194TyrPro: 1.194 ± 0.138
1.295TyrGln: 1.295 ± 0.175
2.185TyrArg: 2.185 ± 0.233
2.711TyrSer: 2.711 ± 0.223
2.974TyrThr: 2.974 ± 0.247
3.217TyrVal: 3.217 ± 0.269
0.587TyrTrp: 0.587 ± 0.107
2.043TyrTyr: 2.043 ± 0.23
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 293 proteins (49433 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski