Desulfococcus oleovorans (strain DSM 6200 / JCM 39069 / Hxd3)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; Desulfococcus; Desulfococcus oleovorans

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3255 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A9A0Q7|A9A0Q7_DESOH Response regulator receiver protein OS=Desulfococcus oleovorans (strain DSM 6200 / JCM 39069 / Hxd3) OX=96561 GN=Dole_3271 PE=4 SV=1
MM1 pKa = 7.62PMNYY5 pKa = 9.49HH6 pKa = 6.83KK7 pKa = 10.21KK8 pKa = 6.45TTAGVRR14 pKa = 11.84NRR16 pKa = 11.84ILWAGLIPLVLCALFVLPAGATDD39 pKa = 3.51IEE41 pKa = 4.55TTPRR45 pKa = 11.84YY46 pKa = 8.41TFVGNVNFVGTGGSLRR62 pKa = 11.84TRR64 pKa = 11.84PNAGWWADD72 pKa = 3.41PCRR75 pKa = 11.84ITTTSTEE82 pKa = 4.09ALSGIPAGATIRR94 pKa = 11.84AAYY97 pKa = 9.5LYY99 pKa = 8.32WARR102 pKa = 11.84SGNAADD108 pKa = 4.15NQVSLNGNTVNADD121 pKa = 3.63RR122 pKa = 11.84PFDD125 pKa = 3.55EE126 pKa = 4.59TFVNGGTTYY135 pKa = 11.13RR136 pKa = 11.84FFSGMADD143 pKa = 3.13VTSIVNTTRR152 pKa = 11.84NGNYY156 pKa = 9.1TFGGLTISAGNPWCGVQAVVGGWALVVIYY185 pKa = 10.23EE186 pKa = 4.26DD187 pKa = 3.56ASEE190 pKa = 4.25KK191 pKa = 10.58EE192 pKa = 4.16RR193 pKa = 11.84VINIYY198 pKa = 10.87DD199 pKa = 3.96GFQYY203 pKa = 10.53FRR205 pKa = 11.84DD206 pKa = 3.6SDD208 pKa = 3.87IVLTPSNFVVSSASGIDD225 pKa = 3.38GKK227 pKa = 10.47IGHH230 pKa = 6.98ISWEE234 pKa = 4.18GDD236 pKa = 3.29PTLTGANEE244 pKa = 3.91YY245 pKa = 10.86LRR247 pKa = 11.84VNGTTLTDD255 pKa = 3.75ASNSATDD262 pKa = 3.58QYY264 pKa = 11.74NSTFSHH270 pKa = 7.12LGTLVSSPTYY280 pKa = 10.62GVDD283 pKa = 3.61FDD285 pKa = 4.76IYY287 pKa = 10.76DD288 pKa = 3.53ISSLLSEE295 pKa = 4.86GDD297 pKa = 3.53SSLSSVYY304 pKa = 10.41SAAGDD309 pKa = 3.78LVILSAEE316 pKa = 4.44IISVTCQAADD326 pKa = 3.57LSISKK331 pKa = 7.54THH333 pKa = 6.12SGGFVSGGTGDD344 pKa = 3.54FTITVTNSGPNSEE357 pKa = 4.51TGTVTVTDD365 pKa = 3.85VLPAGLTYY373 pKa = 10.9SSFSGTGWAVDD384 pKa = 3.42TSGAPTIVFTYY395 pKa = 10.14DD396 pKa = 3.33CSASPLPPGNSLPALTLTVNVGAAAVPNVSNTASVDD432 pKa = 3.13SDD434 pKa = 3.75TTFDD438 pKa = 5.14PDD440 pKa = 3.45TSDD443 pKa = 4.68NSDD446 pKa = 3.33TDD448 pKa = 3.84TVTVSAAAPDD458 pKa = 4.2LLVMKK463 pKa = 9.36TVTTFSDD470 pKa = 3.62PTGGANPKK478 pKa = 9.65AIPGAVMIYY487 pKa = 9.21TIQVTNQGAGPVDD500 pKa = 3.68ADD502 pKa = 4.51SINITDD508 pKa = 6.15AIPANTKK515 pKa = 10.61LFVGDD520 pKa = 4.77LGDD523 pKa = 3.96GPIVFLDD530 pKa = 4.23GSAYY534 pKa = 10.39GGTDD538 pKa = 3.18SGLDD542 pKa = 3.47PYY544 pKa = 11.58DD545 pKa = 4.83YY546 pKa = 10.76IALDD550 pKa = 3.98NDD552 pKa = 3.7TDD554 pKa = 4.16DD555 pKa = 5.96LGFSSDD561 pKa = 3.26NGATFTYY568 pKa = 10.27HH569 pKa = 6.23PTPDD573 pKa = 4.87GDD575 pKa = 4.35DD576 pKa = 3.54CDD578 pKa = 4.95EE579 pKa = 4.34NVTDD583 pKa = 4.86ILVNPKK589 pKa = 10.74GPMDD593 pKa = 4.36GAASGNTPSFQVRR606 pKa = 11.84FRR608 pKa = 11.84VQVEE612 pKa = 3.79

Molecular weight: 63.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8ZS42|A8ZS42_DESOH Putative transcriptional regulator OS=Desulfococcus oleovorans (strain DSM 6200 / JCM 39069 / Hxd3) OX=96561 GN=Dole_0250 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.32QPSNVKK11 pKa = 10.02RR12 pKa = 11.84ARR14 pKa = 11.84KK15 pKa = 8.85HH16 pKa = 4.5GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.05QGRR28 pKa = 11.84SILKK32 pKa = 9.3RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.67GRR39 pKa = 11.84KK40 pKa = 8.88RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3255

0

3255

1152799

34

12741

354.2

39.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.808 ± 0.053

1.33 ± 0.023

5.926 ± 0.057

5.963 ± 0.046

4.337 ± 0.03

7.829 ± 0.069

2.08 ± 0.02

6.015 ± 0.031

4.988 ± 0.057

9.493 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.757 ± 0.03

3.414 ± 0.034

4.673 ± 0.039

3.056 ± 0.023

5.909 ± 0.058

5.281 ± 0.031

5.64 ± 0.068

7.382 ± 0.039

1.155 ± 0.018

2.967 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski