Amino acid dipepetide frequency for Staphylococcus phage 3 AJ-2017

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.71AlaAla: 1.71 ± 0.927
0.466AlaCys: 0.466 ± 0.192
2.021AlaAsp: 2.021 ± 0.373
3.498AlaGlu: 3.498 ± 0.645
2.487AlaPhe: 2.487 ± 0.651
4.042AlaGly: 4.042 ± 0.634
0.311AlaHis: 0.311 ± 0.196
4.508AlaIle: 4.508 ± 0.7
4.586AlaLys: 4.586 ± 0.483
5.441AlaLeu: 5.441 ± 0.792
1.71AlaMet: 1.71 ± 0.421
3.731AlaAsn: 3.731 ± 0.608
1.321AlaPro: 1.321 ± 0.288
2.487AlaGln: 2.487 ± 0.487
3.187AlaArg: 3.187 ± 0.513
3.576AlaSer: 3.576 ± 0.521
3.809AlaThr: 3.809 ± 0.63
2.721AlaVal: 2.721 ± 0.5
0.466AlaTrp: 0.466 ± 0.174
2.332AlaTyr: 2.332 ± 0.344
0.0AlaXaa: 0.0 ± 0.0
Cys
0.389CysAla: 0.389 ± 0.179
0.078CysCys: 0.078 ± 0.073
0.155CysAsp: 0.155 ± 0.174
0.155CysGlu: 0.155 ± 0.112
0.155CysPhe: 0.155 ± 0.112
0.544CysGly: 0.544 ± 0.267
0.155CysHis: 0.155 ± 0.117
0.855CysIle: 0.855 ± 0.291
0.466CysLys: 0.466 ± 0.196
0.311CysLeu: 0.311 ± 0.14
0.0CysMet: 0.0 ± 0.0
0.233CysAsn: 0.233 ± 0.15
0.0CysPro: 0.0 ± 0.0
0.078CysGln: 0.078 ± 0.078
0.389CysArg: 0.389 ± 0.192
0.466CysSer: 0.466 ± 0.205
0.389CysThr: 0.389 ± 0.159
0.233CysVal: 0.233 ± 0.133
0.0CysTrp: 0.0 ± 0.0
0.389CysTyr: 0.389 ± 0.174
0.0CysXaa: 0.0 ± 0.0
Asp
3.187AspAla: 3.187 ± 0.418
0.233AspCys: 0.233 ± 0.133
4.042AspAsp: 4.042 ± 0.58
5.519AspGlu: 5.519 ± 0.752
2.798AspPhe: 2.798 ± 0.432
4.586AspGly: 4.586 ± 0.643
1.01AspHis: 1.01 ± 0.297
4.353AspIle: 4.353 ± 0.646
5.363AspLys: 5.363 ± 0.79
5.052AspLeu: 5.052 ± 0.681
1.555AspMet: 1.555 ± 0.313
4.275AspAsn: 4.275 ± 0.59
1.477AspPro: 1.477 ± 0.419
0.777AspGln: 0.777 ± 0.278
2.487AspArg: 2.487 ± 0.493
3.109AspSer: 3.109 ± 0.523
3.653AspThr: 3.653 ± 0.682
3.809AspVal: 3.809 ± 0.707
0.389AspTrp: 0.389 ± 0.198
2.954AspTyr: 2.954 ± 0.375
0.0AspXaa: 0.0 ± 0.0
Glu
4.197GluAla: 4.197 ± 0.646
0.389GluCys: 0.389 ± 0.168
3.498GluAsp: 3.498 ± 0.507
6.218GluGlu: 6.218 ± 0.986
3.109GluPhe: 3.109 ± 0.432
2.487GluGly: 2.487 ± 0.368
1.477GluHis: 1.477 ± 0.354
6.918GluIle: 6.918 ± 1.079
7.151GluLys: 7.151 ± 0.898
7.307GluLeu: 7.307 ± 0.934
2.41GluMet: 2.41 ± 0.472
5.208GluAsn: 5.208 ± 0.628
2.176GluPro: 2.176 ± 0.405
3.809GluGln: 3.809 ± 0.587
3.731GluArg: 3.731 ± 0.641
4.664GluSer: 4.664 ± 0.632
3.265GluThr: 3.265 ± 0.482
4.586GluVal: 4.586 ± 0.718
0.855GluTrp: 0.855 ± 0.276
3.42GluTyr: 3.42 ± 0.61
0.0GluXaa: 0.0 ± 0.0
Phe
2.176PheAla: 2.176 ± 0.458
0.311PheCys: 0.311 ± 0.142
3.031PheAsp: 3.031 ± 0.59
2.876PheGlu: 2.876 ± 0.69
1.088PhePhe: 1.088 ± 0.291
2.487PheGly: 2.487 ± 0.535
0.7PheHis: 0.7 ± 0.217
3.109PheIle: 3.109 ± 0.585
4.12PheLys: 4.12 ± 0.493
2.954PheLeu: 2.954 ± 0.559
1.244PheMet: 1.244 ± 0.34
3.809PheAsn: 3.809 ± 0.607
1.088PhePro: 1.088 ± 0.307
0.933PheGln: 0.933 ± 0.262
1.321PheArg: 1.321 ± 0.268
2.254PheSer: 2.254 ± 0.457
2.487PheThr: 2.487 ± 0.471
2.099PheVal: 2.099 ± 0.473
0.155PheTrp: 0.155 ± 0.086
1.321PheTyr: 1.321 ± 0.312
0.0PheXaa: 0.0 ± 0.0
Gly
3.031GlyAla: 3.031 ± 0.728
0.311GlyCys: 0.311 ± 0.156
2.721GlyAsp: 2.721 ± 0.529
2.721GlyGlu: 2.721 ± 0.497
2.565GlyPhe: 2.565 ± 0.554
3.109GlyGly: 3.109 ± 0.79
1.555GlyHis: 1.555 ± 0.354
4.508GlyIle: 4.508 ± 0.815
5.441GlyLys: 5.441 ± 0.848
5.519GlyLeu: 5.519 ± 0.8
1.01GlyMet: 1.01 ± 0.323
3.42GlyAsn: 3.42 ± 0.559
0.933GlyPro: 0.933 ± 0.337
2.643GlyGln: 2.643 ± 0.542
2.332GlyArg: 2.332 ± 0.492
3.187GlySer: 3.187 ± 0.421
3.342GlyThr: 3.342 ± 0.49
2.954GlyVal: 2.954 ± 0.622
1.166GlyTrp: 1.166 ± 0.365
3.031GlyTyr: 3.031 ± 0.467
0.0GlyXaa: 0.0 ± 0.0
His
0.855HisAla: 0.855 ± 0.292
0.155HisCys: 0.155 ± 0.123
0.855HisAsp: 0.855 ± 0.26
1.555HisGlu: 1.555 ± 0.359
1.088HisPhe: 1.088 ± 0.247
0.7HisGly: 0.7 ± 0.294
0.389HisHis: 0.389 ± 0.206
1.788HisIle: 1.788 ± 0.413
1.088HisLys: 1.088 ± 0.297
1.477HisLeu: 1.477 ± 0.312
0.389HisMet: 0.389 ± 0.198
1.166HisAsn: 1.166 ± 0.317
0.311HisPro: 0.311 ± 0.167
0.777HisGln: 0.777 ± 0.251
0.933HisArg: 0.933 ± 0.216
1.01HisSer: 1.01 ± 0.247
1.166HisThr: 1.166 ± 0.339
1.166HisVal: 1.166 ± 0.312
0.155HisTrp: 0.155 ± 0.114
0.933HisTyr: 0.933 ± 0.305
0.0HisXaa: 0.0 ± 0.0
Ile
5.052IleAla: 5.052 ± 0.649
0.311IleCys: 0.311 ± 0.149
5.519IleAsp: 5.519 ± 0.701
6.296IleGlu: 6.296 ± 0.85
2.876IlePhe: 2.876 ± 0.494
3.887IleGly: 3.887 ± 0.478
1.477IleHis: 1.477 ± 0.424
4.819IleIle: 4.819 ± 0.578
8.317IleLys: 8.317 ± 0.793
5.363IleLeu: 5.363 ± 0.642
2.176IleMet: 2.176 ± 0.416
6.218IleAsn: 6.218 ± 1.118
1.943IlePro: 1.943 ± 0.375
3.576IleGln: 3.576 ± 0.538
3.031IleArg: 3.031 ± 0.623
4.12IleSer: 4.12 ± 0.599
4.819IleThr: 4.819 ± 0.495
4.664IleVal: 4.664 ± 0.554
0.933IleTrp: 0.933 ± 0.339
2.487IleTyr: 2.487 ± 0.483
0.0IleXaa: 0.0 ± 0.0
Lys
5.208LysAla: 5.208 ± 0.687
0.155LysCys: 0.155 ± 0.108
5.363LysAsp: 5.363 ± 0.512
8.162LysGlu: 8.162 ± 0.888
3.809LysPhe: 3.809 ± 0.664
6.296LysGly: 6.296 ± 0.835
1.477LysHis: 1.477 ± 0.375
7.073LysIle: 7.073 ± 0.803
8.473LysLys: 8.473 ± 0.936
7.151LysLeu: 7.151 ± 0.826
2.41LysMet: 2.41 ± 0.396
5.052LysAsn: 5.052 ± 0.69
3.109LysPro: 3.109 ± 0.657
4.586LysGln: 4.586 ± 0.683
3.809LysArg: 3.809 ± 0.393
6.218LysSer: 6.218 ± 0.606
6.374LysThr: 6.374 ± 0.893
5.286LysVal: 5.286 ± 0.692
1.088LysTrp: 1.088 ± 0.303
4.197LysTyr: 4.197 ± 0.591
0.0LysXaa: 0.0 ± 0.0
Leu
4.197LeuAla: 4.197 ± 0.717
0.389LeuCys: 0.389 ± 0.172
6.607LeuAsp: 6.607 ± 0.772
7.773LeuGlu: 7.773 ± 0.795
3.653LeuPhe: 3.653 ± 0.466
3.887LeuGly: 3.887 ± 0.77
1.01LeuHis: 1.01 ± 0.214
5.208LeuIle: 5.208 ± 0.613
8.317LeuLys: 8.317 ± 0.897
6.84LeuLeu: 6.84 ± 0.948
1.555LeuMet: 1.555 ± 0.358
6.063LeuAsn: 6.063 ± 0.608
2.254LeuPro: 2.254 ± 0.425
3.031LeuGln: 3.031 ± 0.449
3.731LeuArg: 3.731 ± 0.559
6.063LeuSer: 6.063 ± 0.729
4.431LeuThr: 4.431 ± 0.706
4.275LeuVal: 4.275 ± 0.56
0.622LeuTrp: 0.622 ± 0.204
2.487LeuTyr: 2.487 ± 0.369
0.0LeuXaa: 0.0 ± 0.0
Met
1.166MetAla: 1.166 ± 0.324
0.155MetCys: 0.155 ± 0.105
1.088MetAsp: 1.088 ± 0.256
2.021MetGlu: 2.021 ± 0.354
1.01MetPhe: 1.01 ± 0.283
1.01MetGly: 1.01 ± 0.372
0.311MetHis: 0.311 ± 0.17
1.866MetIle: 1.866 ± 0.347
2.487MetLys: 2.487 ± 0.395
2.41MetLeu: 2.41 ± 0.393
0.622MetMet: 0.622 ± 0.233
1.166MetAsn: 1.166 ± 0.384
1.321MetPro: 1.321 ± 0.355
0.933MetGln: 0.933 ± 0.305
1.166MetArg: 1.166 ± 0.367
1.71MetSer: 1.71 ± 0.315
1.71MetThr: 1.71 ± 0.384
1.632MetVal: 1.632 ± 0.383
0.389MetTrp: 0.389 ± 0.174
1.166MetTyr: 1.166 ± 0.331
0.0MetXaa: 0.0 ± 0.0
Asn
4.042AsnAla: 4.042 ± 0.553
0.155AsnCys: 0.155 ± 0.098
4.586AsnAsp: 4.586 ± 0.743
5.441AsnGlu: 5.441 ± 0.791
1.71AsnPhe: 1.71 ± 0.355
5.052AsnGly: 5.052 ± 0.714
1.088AsnHis: 1.088 ± 0.331
4.819AsnIle: 4.819 ± 0.501
6.141AsnLys: 6.141 ± 0.827
5.208AsnLeu: 5.208 ± 0.659
1.477AsnMet: 1.477 ± 0.292
4.975AsnAsn: 4.975 ± 0.667
2.41AsnPro: 2.41 ± 0.287
3.342AsnGln: 3.342 ± 0.543
2.643AsnArg: 2.643 ± 0.435
3.031AsnSer: 3.031 ± 0.548
3.731AsnThr: 3.731 ± 0.523
4.12AsnVal: 4.12 ± 0.586
1.166AsnTrp: 1.166 ± 0.377
2.876AsnTyr: 2.876 ± 0.42
0.0AsnXaa: 0.0 ± 0.0
Pro
1.321ProAla: 1.321 ± 0.441
0.0ProCys: 0.0 ± 0.0
1.477ProAsp: 1.477 ± 0.389
1.71ProGlu: 1.71 ± 0.291
1.088ProPhe: 1.088 ± 0.296
1.166ProGly: 1.166 ± 0.279
0.7ProHis: 0.7 ± 0.264
2.721ProIle: 2.721 ± 0.445
2.41ProLys: 2.41 ± 0.568
1.943ProLeu: 1.943 ± 0.453
0.855ProMet: 0.855 ± 0.252
1.788ProAsn: 1.788 ± 0.367
0.777ProPro: 0.777 ± 0.187
0.777ProGln: 0.777 ± 0.236
0.933ProArg: 0.933 ± 0.276
1.477ProSer: 1.477 ± 0.428
1.866ProThr: 1.866 ± 0.432
1.788ProVal: 1.788 ± 0.357
0.233ProTrp: 0.233 ± 0.122
1.01ProTyr: 1.01 ± 0.299
0.0ProXaa: 0.0 ± 0.0
Gln
3.109GlnAla: 3.109 ± 0.509
0.466GlnCys: 0.466 ± 0.18
2.254GlnAsp: 2.254 ± 0.408
3.187GlnGlu: 3.187 ± 0.718
1.01GlnPhe: 1.01 ± 0.313
1.71GlnGly: 1.71 ± 0.377
0.855GlnHis: 0.855 ± 0.291
2.487GlnIle: 2.487 ± 0.396
3.731GlnLys: 3.731 ± 0.6
3.42GlnLeu: 3.42 ± 0.574
1.088GlnMet: 1.088 ± 0.279
2.643GlnAsn: 2.643 ± 0.436
0.777GlnPro: 0.777 ± 0.232
1.788GlnGln: 1.788 ± 0.483
2.021GlnArg: 2.021 ± 0.481
2.41GlnSer: 2.41 ± 0.55
1.866GlnThr: 1.866 ± 0.354
2.176GlnVal: 2.176 ± 0.434
0.233GlnTrp: 0.233 ± 0.143
2.41GlnTyr: 2.41 ± 0.426
0.0GlnXaa: 0.0 ± 0.0
Arg
2.798ArgAla: 2.798 ± 0.458
0.389ArgCys: 0.389 ± 0.193
2.41ArgAsp: 2.41 ± 0.439
3.731ArgGlu: 3.731 ± 0.598
1.866ArgPhe: 1.866 ± 0.351
1.632ArgGly: 1.632 ± 0.395
0.855ArgHis: 0.855 ± 0.221
3.187ArgIle: 3.187 ± 0.552
3.42ArgLys: 3.42 ± 0.576
3.887ArgLeu: 3.887 ± 0.569
0.933ArgMet: 0.933 ± 0.307
2.876ArgAsn: 2.876 ± 0.392
0.7ArgPro: 0.7 ± 0.231
1.321ArgGln: 1.321 ± 0.399
2.254ArgArg: 2.254 ± 0.442
2.332ArgSer: 2.332 ± 0.43
2.798ArgThr: 2.798 ± 0.499
2.565ArgVal: 2.565 ± 0.44
0.544ArgTrp: 0.544 ± 0.207
2.099ArgTyr: 2.099 ± 0.65
0.0ArgXaa: 0.0 ± 0.0
Ser
3.187SerAla: 3.187 ± 0.647
0.233SerCys: 0.233 ± 0.131
4.12SerAsp: 4.12 ± 0.657
4.664SerGlu: 4.664 ± 0.831
2.487SerPhe: 2.487 ± 0.533
3.342SerGly: 3.342 ± 0.677
1.477SerHis: 1.477 ± 0.355
5.908SerIle: 5.908 ± 0.517
5.985SerLys: 5.985 ± 0.986
4.12SerLeu: 4.12 ± 0.573
1.399SerMet: 1.399 ± 0.341
4.897SerAsn: 4.897 ± 0.597
0.855SerPro: 0.855 ± 0.246
2.176SerGln: 2.176 ± 0.481
2.176SerArg: 2.176 ± 0.398
3.42SerSer: 3.42 ± 0.608
3.887SerThr: 3.887 ± 0.491
2.565SerVal: 2.565 ± 0.562
0.466SerTrp: 0.466 ± 0.171
2.798SerTyr: 2.798 ± 0.551
0.0SerXaa: 0.0 ± 0.0
Thr
3.42ThrAla: 3.42 ± 0.429
0.389ThrCys: 0.389 ± 0.18
4.431ThrAsp: 4.431 ± 0.841
4.197ThrGlu: 4.197 ± 0.671
1.943ThrPhe: 1.943 ± 0.407
3.731ThrGly: 3.731 ± 0.813
1.555ThrHis: 1.555 ± 0.28
4.975ThrIle: 4.975 ± 0.58
6.141ThrLys: 6.141 ± 0.614
4.353ThrLeu: 4.353 ± 0.567
1.321ThrMet: 1.321 ± 0.299
2.876ThrAsn: 2.876 ± 0.505
1.71ThrPro: 1.71 ± 0.395
1.943ThrGln: 1.943 ± 0.371
2.721ThrArg: 2.721 ± 0.442
4.586ThrSer: 4.586 ± 0.724
3.731ThrThr: 3.731 ± 0.547
3.576ThrVal: 3.576 ± 0.531
1.01ThrTrp: 1.01 ± 0.255
2.643ThrTyr: 2.643 ± 0.618
0.0ThrXaa: 0.0 ± 0.0
Val
2.876ValAla: 2.876 ± 0.434
0.466ValCys: 0.466 ± 0.221
2.798ValAsp: 2.798 ± 0.409
3.498ValGlu: 3.498 ± 0.645
2.41ValPhe: 2.41 ± 0.509
3.653ValGly: 3.653 ± 0.813
0.7ValHis: 0.7 ± 0.252
4.12ValIle: 4.12 ± 0.526
5.752ValLys: 5.752 ± 0.656
4.742ValLeu: 4.742 ± 0.633
1.477ValMet: 1.477 ± 0.461
4.431ValAsn: 4.431 ± 0.497
1.477ValPro: 1.477 ± 0.273
2.021ValGln: 2.021 ± 0.337
1.71ValArg: 1.71 ± 0.444
3.42ValSer: 3.42 ± 0.527
5.13ValThr: 5.13 ± 0.873
3.653ValVal: 3.653 ± 0.605
0.389ValTrp: 0.389 ± 0.147
2.332ValTyr: 2.332 ± 0.516
0.0ValXaa: 0.0 ± 0.0
Trp
0.233TrpAla: 0.233 ± 0.157
0.078TrpCys: 0.078 ± 0.082
0.933TrpAsp: 0.933 ± 0.303
0.544TrpGlu: 0.544 ± 0.169
0.466TrpPhe: 0.466 ± 0.17
0.389TrpGly: 0.389 ± 0.145
0.078TrpHis: 0.078 ± 0.082
1.088TrpIle: 1.088 ± 0.264
0.855TrpLys: 0.855 ± 0.223
1.01TrpLeu: 1.01 ± 0.33
0.466TrpMet: 0.466 ± 0.175
0.933TrpAsn: 0.933 ± 0.357
0.155TrpPro: 0.155 ± 0.096
0.544TrpGln: 0.544 ± 0.178
0.622TrpArg: 0.622 ± 0.189
0.622TrpSer: 0.622 ± 0.197
0.389TrpThr: 0.389 ± 0.131
0.777TrpVal: 0.777 ± 0.261
0.155TrpTrp: 0.155 ± 0.103
0.389TrpTyr: 0.389 ± 0.208
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.254TyrAla: 2.254 ± 0.415
0.311TyrCys: 0.311 ± 0.163
2.721TyrAsp: 2.721 ± 0.46
3.109TyrGlu: 3.109 ± 0.399
1.943TyrPhe: 1.943 ± 0.431
1.788TyrGly: 1.788 ± 0.425
0.777TyrHis: 0.777 ± 0.261
3.576TyrIle: 3.576 ± 0.625
5.052TyrLys: 5.052 ± 0.583
3.887TyrLeu: 3.887 ± 0.624
1.244TyrMet: 1.244 ± 0.31
2.254TyrAsn: 2.254 ± 0.373
1.244TyrPro: 1.244 ± 0.44
2.099TyrGln: 2.099 ± 0.365
1.399TyrArg: 1.399 ± 0.383
2.487TyrSer: 2.487 ± 0.418
2.41TyrThr: 2.41 ± 0.441
2.41TyrVal: 2.41 ± 0.485
0.311TyrTrp: 0.311 ± 0.192
1.943TyrTyr: 1.943 ± 0.571
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (12866 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski