Amino acid dipepetide frequency for Vibrio phage pVco-5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.1AlaAla: 7.1 ± 0.923
0.479AlaCys: 0.479 ± 0.154
4.66AlaAsp: 4.66 ± 0.569
5.749AlaGlu: 5.749 ± 0.622
2.396AlaPhe: 2.396 ± 0.229
4.53AlaGly: 4.53 ± 0.41
1.35AlaHis: 1.35 ± 0.273
4.617AlaIle: 4.617 ± 0.544
6.185AlaLys: 6.185 ± 0.712
6.882AlaLeu: 6.882 ± 0.612
2.526AlaMet: 2.526 ± 0.408
5.009AlaAsn: 5.009 ± 0.655
3.18AlaPro: 3.18 ± 0.395
3.441AlaGln: 3.441 ± 0.46
2.918AlaArg: 2.918 ± 0.434
4.356AlaSer: 4.356 ± 0.355
5.009AlaThr: 5.009 ± 0.863
4.922AlaVal: 4.922 ± 0.616
0.566AlaTrp: 0.566 ± 0.14
2.657AlaTyr: 2.657 ± 0.298
0.0AlaXaa: 0.0 ± 0.0
Cys
0.392CysAla: 0.392 ± 0.149
0.0CysCys: 0.0 ± 0.0
0.61CysAsp: 0.61 ± 0.165
0.436CysGlu: 0.436 ± 0.155
0.523CysPhe: 0.523 ± 0.157
0.653CysGly: 0.653 ± 0.194
0.348CysHis: 0.348 ± 0.131
0.697CysIle: 0.697 ± 0.19
0.697CysLys: 0.697 ± 0.216
0.61CysLeu: 0.61 ± 0.177
0.392CysMet: 0.392 ± 0.136
0.305CysAsn: 0.305 ± 0.138
0.261CysPro: 0.261 ± 0.102
0.392CysGln: 0.392 ± 0.161
0.261CysArg: 0.261 ± 0.126
0.566CysSer: 0.566 ± 0.155
0.436CysThr: 0.436 ± 0.164
0.392CysVal: 0.392 ± 0.144
0.0CysTrp: 0.0 ± 0.0
0.523CysTyr: 0.523 ± 0.166
0.0CysXaa: 0.0 ± 0.0
Asp
6.011AspAla: 6.011 ± 0.6
0.392AspCys: 0.392 ± 0.145
3.702AspAsp: 3.702 ± 0.432
4.922AspGlu: 4.922 ± 0.676
1.742AspPhe: 1.742 ± 0.325
4.399AspGly: 4.399 ± 0.571
0.828AspHis: 0.828 ± 0.212
5.575AspIle: 5.575 ± 0.478
4.748AspLys: 4.748 ± 0.484
3.964AspLeu: 3.964 ± 0.382
2.308AspMet: 2.308 ± 0.268
2.613AspAsn: 2.613 ± 0.408
2.221AspPro: 2.221 ± 0.273
1.176AspGln: 1.176 ± 0.168
2.178AspArg: 2.178 ± 0.212
3.92AspSer: 3.92 ± 0.409
4.53AspThr: 4.53 ± 0.526
4.007AspVal: 4.007 ± 0.44
0.653AspTrp: 0.653 ± 0.195
2.439AspTyr: 2.439 ± 0.236
0.0AspXaa: 0.0 ± 0.0
Glu
5.706GluAla: 5.706 ± 0.627
0.653GluCys: 0.653 ± 0.17
4.356GluAsp: 4.356 ± 0.464
4.486GluGlu: 4.486 ± 0.826
2.831GluPhe: 2.831 ± 0.388
3.789GluGly: 3.789 ± 0.32
1.394GluHis: 1.394 ± 0.308
4.007GluIle: 4.007 ± 0.534
3.484GluLys: 3.484 ± 0.47
7.1GluLeu: 7.1 ± 0.609
1.96GluMet: 1.96 ± 0.272
2.352GluAsn: 2.352 ± 0.323
2.744GluPro: 2.744 ± 0.338
3.615GluGln: 3.615 ± 0.532
2.352GluArg: 2.352 ± 0.403
4.138GluSer: 4.138 ± 0.525
4.486GluThr: 4.486 ± 0.446
5.009GluVal: 5.009 ± 0.448
0.915GluTrp: 0.915 ± 0.229
2.875GluTyr: 2.875 ± 0.381
0.0GluXaa: 0.0 ± 0.0
Phe
2.352PheAla: 2.352 ± 0.271
0.087PheCys: 0.087 ± 0.074
2.396PheAsp: 2.396 ± 0.228
2.004PheGlu: 2.004 ± 0.28
1.481PhePhe: 1.481 ± 0.302
2.657PheGly: 2.657 ± 0.39
0.566PheHis: 0.566 ± 0.152
2.875PheIle: 2.875 ± 0.347
3.049PheLys: 3.049 ± 0.335
1.742PheLeu: 1.742 ± 0.315
0.915PheMet: 0.915 ± 0.236
2.788PheAsn: 2.788 ± 0.323
1.394PhePro: 1.394 ± 0.29
1.045PheGln: 1.045 ± 0.218
1.568PheArg: 1.568 ± 0.261
2.396PheSer: 2.396 ± 0.257
3.049PheThr: 3.049 ± 0.375
2.483PheVal: 2.483 ± 0.284
0.174PheTrp: 0.174 ± 0.082
1.437PheTyr: 1.437 ± 0.301
0.0PheXaa: 0.0 ± 0.0
Gly
4.486GlyAla: 4.486 ± 0.589
0.566GlyCys: 0.566 ± 0.175
3.18GlyAsp: 3.18 ± 0.453
3.267GlyGlu: 3.267 ± 0.39
2.7GlyPhe: 2.7 ± 0.432
3.18GlyGly: 3.18 ± 0.512
1.002GlyHis: 1.002 ± 0.263
4.225GlyIle: 4.225 ± 0.349
4.53GlyLys: 4.53 ± 0.475
4.094GlyLeu: 4.094 ± 0.454
1.699GlyMet: 1.699 ± 0.32
3.092GlyAsn: 3.092 ± 0.509
0.305GlyPro: 0.305 ± 0.099
2.613GlyGln: 2.613 ± 0.393
2.134GlyArg: 2.134 ± 0.301
4.007GlySer: 4.007 ± 0.544
4.835GlyThr: 4.835 ± 0.502
3.92GlyVal: 3.92 ± 0.358
0.915GlyTrp: 0.915 ± 0.197
3.528GlyTyr: 3.528 ± 0.396
0.0GlyXaa: 0.0 ± 0.0
His
1.045HisAla: 1.045 ± 0.257
0.087HisCys: 0.087 ± 0.062
1.176HisAsp: 1.176 ± 0.288
1.22HisGlu: 1.22 ± 0.233
1.045HisPhe: 1.045 ± 0.221
0.871HisGly: 0.871 ± 0.22
0.392HisHis: 0.392 ± 0.141
1.612HisIle: 1.612 ± 0.392
1.307HisLys: 1.307 ± 0.291
1.742HisLeu: 1.742 ± 0.27
0.653HisMet: 0.653 ± 0.168
1.699HisAsn: 1.699 ± 0.231
0.653HisPro: 0.653 ± 0.163
0.871HisGln: 0.871 ± 0.214
1.089HisArg: 1.089 ± 0.211
1.132HisSer: 1.132 ± 0.264
1.35HisThr: 1.35 ± 0.245
1.263HisVal: 1.263 ± 0.29
0.392HisTrp: 0.392 ± 0.14
0.871HisTyr: 0.871 ± 0.208
0.0HisXaa: 0.0 ± 0.0
Ile
4.748IleAla: 4.748 ± 0.387
0.436IleCys: 0.436 ± 0.143
3.746IleAsp: 3.746 ± 0.365
4.66IleGlu: 4.66 ± 0.403
1.394IlePhe: 1.394 ± 0.233
3.397IleGly: 3.397 ± 0.41
1.96IleHis: 1.96 ± 0.366
3.484IleIle: 3.484 ± 0.381
4.704IleLys: 4.704 ± 0.487
4.748IleLeu: 4.748 ± 0.651
1.437IleMet: 1.437 ± 0.228
2.918IleAsn: 2.918 ± 0.337
3.223IlePro: 3.223 ± 0.309
2.831IleGln: 2.831 ± 0.433
3.049IleArg: 3.049 ± 0.361
3.572IleSer: 3.572 ± 0.461
3.876IleThr: 3.876 ± 0.599
3.397IleVal: 3.397 ± 0.318
0.697IleTrp: 0.697 ± 0.182
2.57IleTyr: 2.57 ± 0.347
0.0IleXaa: 0.0 ± 0.0
Lys
6.228LysAla: 6.228 ± 0.676
0.697LysCys: 0.697 ± 0.22
5.27LysAsp: 5.27 ± 0.561
4.791LysGlu: 4.791 ± 0.618
2.047LysPhe: 2.047 ± 0.295
4.094LysGly: 4.094 ± 0.421
1.568LysHis: 1.568 ± 0.329
3.267LysIle: 3.267 ± 0.402
3.441LysLys: 3.441 ± 0.512
6.969LysLeu: 6.969 ± 0.616
2.221LysMet: 2.221 ± 0.289
3.223LysAsn: 3.223 ± 0.327
2.7LysPro: 2.7 ± 0.337
3.659LysGln: 3.659 ± 0.48
2.7LysArg: 2.7 ± 0.395
3.964LysSer: 3.964 ± 0.468
4.312LysThr: 4.312 ± 0.473
4.704LysVal: 4.704 ± 0.443
0.479LysTrp: 0.479 ± 0.136
3.484LysTyr: 3.484 ± 0.422
0.0LysXaa: 0.0 ± 0.0
Leu
6.228LeuAla: 6.228 ± 0.442
0.566LeuCys: 0.566 ± 0.176
5.14LeuAsp: 5.14 ± 0.526
5.183LeuGlu: 5.183 ± 0.441
2.744LeuPhe: 2.744 ± 0.363
4.617LeuGly: 4.617 ± 0.527
2.047LeuHis: 2.047 ± 0.277
4.573LeuIle: 4.573 ± 0.599
5.401LeuLys: 5.401 ± 0.418
6.272LeuLeu: 6.272 ± 0.623
2.308LeuMet: 2.308 ± 0.295
4.617LeuAsn: 4.617 ± 0.371
3.702LeuPro: 3.702 ± 0.404
2.962LeuGln: 2.962 ± 0.385
4.617LeuArg: 4.617 ± 0.403
5.88LeuSer: 5.88 ± 0.701
6.446LeuThr: 6.446 ± 0.607
4.965LeuVal: 4.965 ± 0.392
0.828LeuTrp: 0.828 ± 0.169
2.047LeuTyr: 2.047 ± 0.244
0.0LeuXaa: 0.0 ± 0.0
Met
2.308MetAla: 2.308 ± 0.295
0.218MetCys: 0.218 ± 0.083
1.742MetAsp: 1.742 ± 0.263
2.613MetGlu: 2.613 ± 0.491
1.002MetPhe: 1.002 ± 0.175
1.437MetGly: 1.437 ± 0.252
0.523MetHis: 0.523 ± 0.157
1.568MetIle: 1.568 ± 0.244
2.134MetLys: 2.134 ± 0.283
2.308MetLeu: 2.308 ± 0.344
1.176MetMet: 1.176 ± 0.24
1.132MetAsn: 1.132 ± 0.24
1.132MetPro: 1.132 ± 0.168
1.22MetGln: 1.22 ± 0.223
1.829MetArg: 1.829 ± 0.344
2.004MetSer: 2.004 ± 0.29
2.265MetThr: 2.265 ± 0.27
1.524MetVal: 1.524 ± 0.296
0.479MetTrp: 0.479 ± 0.146
1.045MetTyr: 1.045 ± 0.166
0.0MetXaa: 0.0 ± 0.0
Asn
4.051AsnAla: 4.051 ± 0.456
0.523AsnCys: 0.523 ± 0.139
2.57AsnAsp: 2.57 ± 0.301
3.441AsnGlu: 3.441 ± 0.343
2.047AsnPhe: 2.047 ± 0.287
3.18AsnGly: 3.18 ± 0.474
1.176AsnHis: 1.176 ± 0.219
3.572AsnIle: 3.572 ± 0.583
3.572AsnLys: 3.572 ± 0.343
4.399AsnLeu: 4.399 ± 0.41
1.481AsnMet: 1.481 ± 0.28
3.31AsnAsn: 3.31 ± 0.43
3.18AsnPro: 3.18 ± 0.364
2.657AsnGln: 2.657 ± 0.413
2.221AsnArg: 2.221 ± 0.352
3.354AsnSer: 3.354 ± 0.537
3.92AsnThr: 3.92 ± 0.392
3.615AsnVal: 3.615 ± 0.397
0.479AsnTrp: 0.479 ± 0.141
2.657AsnTyr: 2.657 ± 0.346
0.0AsnXaa: 0.0 ± 0.0
Pro
2.962ProAla: 2.962 ± 0.311
0.436ProCys: 0.436 ± 0.155
2.613ProAsp: 2.613 ± 0.301
3.092ProGlu: 3.092 ± 0.391
1.35ProPhe: 1.35 ± 0.245
0.958ProGly: 0.958 ± 0.176
0.653ProHis: 0.653 ± 0.174
2.091ProIle: 2.091 ± 0.323
2.657ProLys: 2.657 ± 0.357
3.049ProLeu: 3.049 ± 0.349
1.176ProMet: 1.176 ± 0.251
2.918ProAsn: 2.918 ± 0.385
0.784ProPro: 0.784 ± 0.166
1.002ProGln: 1.002 ± 0.194
1.263ProArg: 1.263 ± 0.186
2.308ProSer: 2.308 ± 0.335
3.136ProThr: 3.136 ± 0.444
2.439ProVal: 2.439 ± 0.331
0.305ProTrp: 0.305 ± 0.13
1.481ProTyr: 1.481 ± 0.254
0.0ProXaa: 0.0 ± 0.0
Gln
4.486GlnAla: 4.486 ± 0.654
0.436GlnCys: 0.436 ± 0.139
2.004GlnAsp: 2.004 ± 0.332
4.138GlnGlu: 4.138 ± 0.484
1.263GlnPhe: 1.263 ± 0.191
2.439GlnGly: 2.439 ± 0.306
0.653GlnHis: 0.653 ± 0.196
2.047GlnIle: 2.047 ± 0.29
2.352GlnLys: 2.352 ± 0.391
3.441GlnLeu: 3.441 ± 0.472
1.481GlnMet: 1.481 ± 0.282
1.524GlnAsn: 1.524 ± 0.271
1.263GlnPro: 1.263 ± 0.246
2.265GlnGln: 2.265 ± 0.379
2.221GlnArg: 2.221 ± 0.437
2.221GlnSer: 2.221 ± 0.242
2.134GlnThr: 2.134 ± 0.341
2.352GlnVal: 2.352 ± 0.32
0.566GlnTrp: 0.566 ± 0.181
1.829GlnTyr: 1.829 ± 0.239
0.0GlnXaa: 0.0 ± 0.0
Arg
3.005ArgAla: 3.005 ± 0.318
0.523ArgCys: 0.523 ± 0.179
2.178ArgAsp: 2.178 ± 0.293
2.831ArgGlu: 2.831 ± 0.324
1.481ArgPhe: 1.481 ± 0.313
2.613ArgGly: 2.613 ± 0.327
0.915ArgHis: 0.915 ± 0.19
2.221ArgIle: 2.221 ± 0.286
3.746ArgLys: 3.746 ± 0.416
3.615ArgLeu: 3.615 ± 0.407
1.568ArgMet: 1.568 ± 0.278
2.657ArgAsn: 2.657 ± 0.382
1.132ArgPro: 1.132 ± 0.275
1.524ArgGln: 1.524 ± 0.293
2.091ArgArg: 2.091 ± 0.292
2.788ArgSer: 2.788 ± 0.407
2.744ArgThr: 2.744 ± 0.337
3.005ArgVal: 3.005 ± 0.374
0.348ArgTrp: 0.348 ± 0.106
2.004ArgTyr: 2.004 ± 0.306
0.0ArgXaa: 0.0 ± 0.0
Ser
4.748SerAla: 4.748 ± 0.511
0.61SerCys: 0.61 ± 0.191
4.138SerAsp: 4.138 ± 0.432
3.223SerGlu: 3.223 ± 0.449
2.831SerPhe: 2.831 ± 0.567
4.617SerGly: 4.617 ± 0.485
1.263SerHis: 1.263 ± 0.199
4.051SerIle: 4.051 ± 0.381
4.617SerLys: 4.617 ± 0.533
4.66SerLeu: 4.66 ± 0.561
1.524SerMet: 1.524 ± 0.215
4.138SerAsn: 4.138 ± 0.36
1.786SerPro: 1.786 ± 0.253
1.742SerGln: 1.742 ± 0.199
2.221SerArg: 2.221 ± 0.269
3.876SerSer: 3.876 ± 0.489
3.833SerThr: 3.833 ± 0.318
3.92SerVal: 3.92 ± 0.371
1.002SerTrp: 1.002 ± 0.194
2.918SerTyr: 2.918 ± 0.33
0.0SerXaa: 0.0 ± 0.0
Thr
4.835ThrAla: 4.835 ± 0.48
0.348ThrCys: 0.348 ± 0.136
4.486ThrAsp: 4.486 ± 0.569
4.138ThrGlu: 4.138 ± 0.388
2.396ThrPhe: 2.396 ± 0.263
4.399ThrGly: 4.399 ± 0.493
1.524ThrHis: 1.524 ± 0.285
3.92ThrIle: 3.92 ± 0.368
4.835ThrLys: 4.835 ± 0.46
5.575ThrLeu: 5.575 ± 0.525
1.481ThrMet: 1.481 ± 0.238
4.268ThrAsn: 4.268 ± 0.375
2.613ThrPro: 2.613 ± 0.357
2.831ThrGln: 2.831 ± 0.405
2.875ThrArg: 2.875 ± 0.29
4.399ThrSer: 4.399 ± 0.55
4.53ThrThr: 4.53 ± 0.473
5.009ThrVal: 5.009 ± 0.5
1.002ThrTrp: 1.002 ± 0.233
3.441ThrTyr: 3.441 ± 0.357
0.0ThrXaa: 0.0 ± 0.0
Val
4.704ValAla: 4.704 ± 0.525
0.566ValCys: 0.566 ± 0.172
5.14ValAsp: 5.14 ± 0.429
4.399ValGlu: 4.399 ± 0.541
2.918ValPhe: 2.918 ± 0.321
3.441ValGly: 3.441 ± 0.411
1.132ValHis: 1.132 ± 0.207
3.397ValIle: 3.397 ± 0.39
4.66ValLys: 4.66 ± 0.41
5.183ValLeu: 5.183 ± 0.612
1.699ValMet: 1.699 ± 0.278
3.833ValAsn: 3.833 ± 0.385
2.875ValPro: 2.875 ± 0.373
3.136ValGln: 3.136 ± 0.401
2.788ValArg: 2.788 ± 0.347
3.528ValSer: 3.528 ± 0.39
4.53ValThr: 4.53 ± 0.514
4.443ValVal: 4.443 ± 0.449
0.523ValTrp: 0.523 ± 0.164
2.439ValTyr: 2.439 ± 0.316
0.0ValXaa: 0.0 ± 0.0
Trp
0.697TrpAla: 0.697 ± 0.121
0.131TrpCys: 0.131 ± 0.093
0.697TrpAsp: 0.697 ± 0.17
0.479TrpGlu: 0.479 ± 0.171
0.523TrpPhe: 0.523 ± 0.155
0.523TrpGly: 0.523 ± 0.125
0.348TrpHis: 0.348 ± 0.138
0.697TrpIle: 0.697 ± 0.197
0.74TrpLys: 0.74 ± 0.21
1.307TrpLeu: 1.307 ± 0.268
0.305TrpMet: 0.305 ± 0.111
0.479TrpAsn: 0.479 ± 0.135
0.174TrpPro: 0.174 ± 0.096
0.436TrpGln: 0.436 ± 0.144
0.261TrpArg: 0.261 ± 0.117
0.697TrpSer: 0.697 ± 0.193
0.828TrpThr: 0.828 ± 0.19
1.002TrpVal: 1.002 ± 0.218
0.087TrpTrp: 0.087 ± 0.051
0.523TrpTyr: 0.523 ± 0.164
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.483TyrAla: 2.483 ± 0.252
0.74TyrCys: 0.74 ± 0.206
2.526TyrAsp: 2.526 ± 0.285
3.18TyrGlu: 3.18 ± 0.329
1.742TyrPhe: 1.742 ± 0.273
2.483TyrGly: 2.483 ± 0.315
0.828TyrHis: 0.828 ± 0.17
2.352TyrIle: 2.352 ± 0.326
2.962TyrLys: 2.962 ± 0.443
3.484TyrLeu: 3.484 ± 0.324
1.307TyrMet: 1.307 ± 0.287
2.526TyrAsn: 2.526 ± 0.277
1.35TyrPro: 1.35 ± 0.252
1.699TyrGln: 1.699 ± 0.272
2.352TyrArg: 2.352 ± 0.33
2.613TyrSer: 2.613 ± 0.321
2.657TyrThr: 2.657 ± 0.389
2.962TyrVal: 2.962 ± 0.364
0.523TyrTrp: 0.523 ± 0.165
1.873TyrTyr: 1.873 ± 0.324
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 126 proteins (22960 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski