Amino acid dipepetide frequency for Pseudomonas phage Bjorn

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.287AlaAla: 10.287 ± 1.778
0.634AlaCys: 0.634 ± 0.214
5.848AlaAsp: 5.848 ± 0.767
5.778AlaGlu: 5.778 ± 0.739
2.818AlaPhe: 2.818 ± 0.397
8.385AlaGly: 8.385 ± 1.379
1.621AlaHis: 1.621 ± 0.347
4.51AlaIle: 4.51 ± 0.428
5.637AlaLys: 5.637 ± 0.771
7.821AlaLeu: 7.821 ± 0.905
1.902AlaMet: 1.902 ± 0.34
4.016AlaAsn: 4.016 ± 0.579
3.875AlaPro: 3.875 ± 0.721
6.342AlaGln: 6.342 ± 1.237
4.862AlaArg: 4.862 ± 0.602
5.285AlaSer: 5.285 ± 0.513
5.919AlaThr: 5.919 ± 0.57
6.342AlaVal: 6.342 ± 0.772
1.762AlaTrp: 1.762 ± 0.312
2.959AlaTyr: 2.959 ± 0.486
0.0AlaXaa: 0.0 ± 0.0
Cys
0.775CysAla: 0.775 ± 0.216
0.07CysCys: 0.07 ± 0.059
0.634CysAsp: 0.634 ± 0.215
0.846CysGlu: 0.846 ± 0.256
0.634CysPhe: 0.634 ± 0.186
0.846CysGly: 0.846 ± 0.248
0.423CysHis: 0.423 ± 0.153
0.352CysIle: 0.352 ± 0.167
0.352CysLys: 0.352 ± 0.192
1.127CysLeu: 1.127 ± 0.331
0.07CysMet: 0.07 ± 0.07
0.775CysAsn: 0.775 ± 0.2
0.423CysPro: 0.423 ± 0.169
0.352CysGln: 0.352 ± 0.146
0.423CysArg: 0.423 ± 0.174
0.352CysSer: 0.352 ± 0.147
0.634CysThr: 0.634 ± 0.193
0.352CysVal: 0.352 ± 0.152
0.211CysTrp: 0.211 ± 0.109
0.634CysTyr: 0.634 ± 0.212
0.0CysXaa: 0.0 ± 0.0
Asp
5.707AspAla: 5.707 ± 0.528
0.564AspCys: 0.564 ± 0.24
4.51AspAsp: 4.51 ± 0.93
3.453AspGlu: 3.453 ± 0.54
1.832AspPhe: 1.832 ± 0.353
5.496AspGly: 5.496 ± 0.727
1.621AspHis: 1.621 ± 0.395
3.875AspIle: 3.875 ± 0.57
3.171AspLys: 3.171 ± 0.483
4.651AspLeu: 4.651 ± 0.617
2.396AspMet: 2.396 ± 0.371
2.325AspAsn: 2.325 ± 0.352
4.016AspPro: 4.016 ± 0.71
2.466AspGln: 2.466 ± 0.335
4.51AspArg: 4.51 ± 0.488
2.678AspSer: 2.678 ± 0.389
3.1AspThr: 3.1 ± 0.403
4.721AspVal: 4.721 ± 0.601
1.127AspTrp: 1.127 ± 0.292
1.621AspTyr: 1.621 ± 0.282
0.0AspXaa: 0.0 ± 0.0
Glu
8.033GluAla: 8.033 ± 1.027
0.775GluCys: 0.775 ± 0.228
3.523GluAsp: 3.523 ± 0.511
3.382GluGlu: 3.382 ± 0.613
2.396GluPhe: 2.396 ± 0.389
4.298GluGly: 4.298 ± 0.475
0.916GluHis: 0.916 ± 0.28
3.03GluIle: 3.03 ± 0.415
3.03GluLys: 3.03 ± 0.433
6.623GluLeu: 6.623 ± 0.762
1.762GluMet: 1.762 ± 0.351
2.255GluAsn: 2.255 ± 0.31
1.902GluPro: 1.902 ± 0.463
2.889GluGln: 2.889 ± 0.432
2.889GluArg: 2.889 ± 0.317
2.959GluSer: 2.959 ± 0.504
2.818GluThr: 2.818 ± 0.361
3.875GluVal: 3.875 ± 0.555
0.634GluTrp: 0.634 ± 0.222
2.184GluTyr: 2.184 ± 0.522
0.0GluXaa: 0.0 ± 0.0
Phe
2.043PheAla: 2.043 ± 0.354
0.775PheCys: 0.775 ± 0.261
1.973PheAsp: 1.973 ± 0.338
2.537PheGlu: 2.537 ± 0.462
1.339PhePhe: 1.339 ± 0.328
3.312PheGly: 3.312 ± 0.448
0.493PheHis: 0.493 ± 0.177
1.621PheIle: 1.621 ± 0.294
1.55PheLys: 1.55 ± 0.321
3.241PheLeu: 3.241 ± 0.383
1.127PheMet: 1.127 ± 0.335
1.621PheAsn: 1.621 ± 0.319
2.114PhePro: 2.114 ± 0.444
1.339PheGln: 1.339 ± 0.293
1.902PheArg: 1.902 ± 0.337
1.621PheSer: 1.621 ± 0.436
2.043PheThr: 2.043 ± 0.307
2.325PheVal: 2.325 ± 0.399
0.493PheTrp: 0.493 ± 0.177
0.846PheTyr: 0.846 ± 0.273
0.0PheXaa: 0.0 ± 0.0
Gly
8.455GlyAla: 8.455 ± 1.056
0.775GlyCys: 0.775 ± 0.189
4.791GlyAsp: 4.791 ± 0.514
4.298GlyGlu: 4.298 ± 0.481
3.1GlyPhe: 3.1 ± 0.445
9.09GlyGly: 9.09 ± 1.176
1.339GlyHis: 1.339 ± 0.294
3.805GlyIle: 3.805 ± 0.541
4.721GlyLys: 4.721 ± 0.553
6.06GlyLeu: 6.06 ± 0.528
2.325GlyMet: 2.325 ± 0.514
3.453GlyAsn: 3.453 ± 0.484
2.537GlyPro: 2.537 ± 0.528
3.805GlyGln: 3.805 ± 0.565
4.862GlyArg: 4.862 ± 0.592
4.862GlySer: 4.862 ± 0.79
4.58GlyThr: 4.58 ± 0.755
6.13GlyVal: 6.13 ± 0.65
1.057GlyTrp: 1.057 ± 0.213
3.382GlyTyr: 3.382 ± 0.459
0.0GlyXaa: 0.0 ± 0.0
His
1.832HisAla: 1.832 ± 0.299
0.282HisCys: 0.282 ± 0.135
1.268HisAsp: 1.268 ± 0.282
1.127HisGlu: 1.127 ± 0.28
0.423HisPhe: 0.423 ± 0.159
1.339HisGly: 1.339 ± 0.318
0.423HisHis: 0.423 ± 0.154
0.986HisIle: 0.986 ± 0.255
0.916HisLys: 0.916 ± 0.257
1.832HisLeu: 1.832 ± 0.368
0.423HisMet: 0.423 ± 0.113
0.986HisAsn: 0.986 ± 0.349
1.268HisPro: 1.268 ± 0.294
0.634HisGln: 0.634 ± 0.178
1.339HisArg: 1.339 ± 0.335
0.846HisSer: 0.846 ± 0.255
0.846HisThr: 0.846 ± 0.274
1.339HisVal: 1.339 ± 0.347
0.423HisTrp: 0.423 ± 0.158
0.916HisTyr: 0.916 ± 0.311
0.0HisXaa: 0.0 ± 0.0
Ile
4.228IleAla: 4.228 ± 0.699
0.564IleCys: 0.564 ± 0.189
3.312IleAsp: 3.312 ± 0.513
2.678IleGlu: 2.678 ± 0.471
1.621IlePhe: 1.621 ± 0.312
4.932IleGly: 4.932 ± 0.524
0.986IleHis: 0.986 ± 0.326
2.184IleIle: 2.184 ± 0.455
2.466IleLys: 2.466 ± 0.508
3.805IleLeu: 3.805 ± 0.459
1.198IleMet: 1.198 ± 0.24
2.114IleAsn: 2.114 ± 0.355
2.466IlePro: 2.466 ± 0.488
1.691IleGln: 1.691 ± 0.318
3.241IleArg: 3.241 ± 0.41
1.973IleSer: 1.973 ± 0.431
2.396IleThr: 2.396 ± 0.38
3.312IleVal: 3.312 ± 0.598
0.775IleTrp: 0.775 ± 0.236
1.621IleTyr: 1.621 ± 0.323
0.0IleXaa: 0.0 ± 0.0
Lys
5.919LysAla: 5.919 ± 0.906
0.423LysCys: 0.423 ± 0.282
3.171LysAsp: 3.171 ± 0.553
3.171LysGlu: 3.171 ± 0.456
1.902LysPhe: 1.902 ± 0.441
4.369LysGly: 4.369 ± 0.563
1.339LysHis: 1.339 ± 0.294
2.748LysIle: 2.748 ± 0.448
2.959LysLys: 2.959 ± 0.494
4.932LysLeu: 4.932 ± 0.473
1.268LysMet: 1.268 ± 0.306
1.973LysAsn: 1.973 ± 0.348
2.466LysPro: 2.466 ± 0.371
1.973LysGln: 1.973 ± 0.385
2.818LysArg: 2.818 ± 0.568
2.466LysSer: 2.466 ± 0.387
3.241LysThr: 3.241 ± 0.41
4.439LysVal: 4.439 ± 0.615
1.127LysTrp: 1.127 ± 0.289
1.55LysTyr: 1.55 ± 0.342
0.0LysXaa: 0.0 ± 0.0
Leu
7.117LeuAla: 7.117 ± 0.792
1.127LeuCys: 1.127 ± 0.298
5.496LeuAsp: 5.496 ± 0.609
5.567LeuGlu: 5.567 ± 0.78
2.818LeuPhe: 2.818 ± 0.512
5.214LeuGly: 5.214 ± 0.623
1.268LeuHis: 1.268 ± 0.33
3.875LeuIle: 3.875 ± 0.495
4.791LeuLys: 4.791 ± 0.696
6.976LeuLeu: 6.976 ± 0.849
2.114LeuMet: 2.114 ± 0.427
5.355LeuAsn: 5.355 ± 0.526
3.523LeuPro: 3.523 ± 0.565
3.312LeuGln: 3.312 ± 0.475
5.355LeuArg: 5.355 ± 0.729
5.355LeuSer: 5.355 ± 0.559
5.285LeuThr: 5.285 ± 0.516
5.003LeuVal: 5.003 ± 0.546
1.55LeuTrp: 1.55 ± 0.329
2.607LeuTyr: 2.607 ± 0.489
0.0LeuXaa: 0.0 ± 0.0
Met
3.805MetAla: 3.805 ± 0.693
0.352MetCys: 0.352 ± 0.16
1.409MetAsp: 1.409 ± 0.256
1.127MetGlu: 1.127 ± 0.271
1.198MetPhe: 1.198 ± 0.262
2.678MetGly: 2.678 ± 0.472
0.564MetHis: 0.564 ± 0.267
0.846MetIle: 0.846 ± 0.278
2.255MetLys: 2.255 ± 0.434
1.902MetLeu: 1.902 ± 0.268
0.775MetMet: 0.775 ± 0.226
0.564MetAsn: 0.564 ± 0.197
0.986MetPro: 0.986 ± 0.232
1.127MetGln: 1.127 ± 0.253
1.621MetArg: 1.621 ± 0.406
2.255MetSer: 2.255 ± 0.376
2.114MetThr: 2.114 ± 0.421
1.339MetVal: 1.339 ± 0.302
0.493MetTrp: 0.493 ± 0.198
0.705MetTyr: 0.705 ± 0.188
0.0MetXaa: 0.0 ± 0.0
Asn
4.298AsnAla: 4.298 ± 0.523
0.564AsnCys: 0.564 ± 0.172
2.889AsnAsp: 2.889 ± 0.355
2.325AsnGlu: 2.325 ± 0.418
1.832AsnPhe: 1.832 ± 0.415
4.51AsnGly: 4.51 ± 0.662
1.127AsnHis: 1.127 ± 0.266
2.255AsnIle: 2.255 ± 0.373
1.832AsnLys: 1.832 ± 0.322
3.382AsnLeu: 3.382 ± 0.443
1.127AsnMet: 1.127 ± 0.289
2.325AsnAsn: 2.325 ± 0.508
2.678AsnPro: 2.678 ± 0.46
2.255AsnGln: 2.255 ± 0.377
2.466AsnArg: 2.466 ± 0.395
2.255AsnSer: 2.255 ± 0.353
2.748AsnThr: 2.748 ± 0.427
2.678AsnVal: 2.678 ± 0.499
1.198AsnTrp: 1.198 ± 0.286
1.762AsnTyr: 1.762 ± 0.359
0.0AsnXaa: 0.0 ± 0.0
Pro
3.523ProAla: 3.523 ± 0.42
0.141ProCys: 0.141 ± 0.105
4.016ProAsp: 4.016 ± 0.514
4.228ProGlu: 4.228 ± 0.791
2.678ProPhe: 2.678 ± 0.369
3.453ProGly: 3.453 ± 0.475
0.634ProHis: 0.634 ± 0.22
1.198ProIle: 1.198 ± 0.328
2.607ProLys: 2.607 ± 0.353
3.1ProLeu: 3.1 ± 0.523
1.409ProMet: 1.409 ± 0.256
2.255ProAsn: 2.255 ± 0.467
2.043ProPro: 2.043 ± 0.378
1.902ProGln: 1.902 ± 0.444
1.691ProArg: 1.691 ± 0.338
2.043ProSer: 2.043 ± 0.345
3.382ProThr: 3.382 ± 0.714
3.03ProVal: 3.03 ± 0.423
0.282ProTrp: 0.282 ± 0.126
1.621ProTyr: 1.621 ± 0.43
0.0ProXaa: 0.0 ± 0.0
Gln
6.412GlnAla: 6.412 ± 0.833
0.282GlnCys: 0.282 ± 0.13
2.396GlnAsp: 2.396 ± 0.42
2.889GlnGlu: 2.889 ± 0.434
1.127GlnPhe: 1.127 ± 0.301
4.087GlnGly: 4.087 ± 0.683
0.634GlnHis: 0.634 ± 0.226
2.325GlnIle: 2.325 ± 0.393
2.184GlnLys: 2.184 ± 0.537
3.312GlnLeu: 3.312 ± 0.686
1.691GlnMet: 1.691 ± 0.36
1.902GlnAsn: 1.902 ± 0.467
1.339GlnPro: 1.339 ± 0.311
2.959GlnGln: 2.959 ± 0.686
2.255GlnArg: 2.255 ± 0.403
2.043GlnSer: 2.043 ± 0.446
2.325GlnThr: 2.325 ± 0.44
2.466GlnVal: 2.466 ± 0.409
0.493GlnTrp: 0.493 ± 0.221
1.762GlnTyr: 1.762 ± 0.33
0.0GlnXaa: 0.0 ± 0.0
Arg
4.087ArgAla: 4.087 ± 0.572
0.282ArgCys: 0.282 ± 0.14
3.312ArgAsp: 3.312 ± 0.537
3.453ArgGlu: 3.453 ± 0.487
2.043ArgPhe: 2.043 ± 0.296
3.523ArgGly: 3.523 ± 0.501
0.846ArgHis: 0.846 ± 0.252
3.664ArgIle: 3.664 ± 0.45
3.03ArgLys: 3.03 ± 0.46
4.862ArgLeu: 4.862 ± 0.948
2.255ArgMet: 2.255 ± 0.387
3.594ArgAsn: 3.594 ± 0.543
2.678ArgPro: 2.678 ± 0.395
2.748ArgGln: 2.748 ± 0.494
2.678ArgArg: 2.678 ± 0.522
3.171ArgSer: 3.171 ± 0.451
2.607ArgThr: 2.607 ± 0.441
3.734ArgVal: 3.734 ± 0.521
0.634ArgTrp: 0.634 ± 0.219
1.973ArgTyr: 1.973 ± 0.325
0.0ArgXaa: 0.0 ± 0.0
Ser
4.157SerAla: 4.157 ± 0.559
0.282SerCys: 0.282 ± 0.226
3.453SerAsp: 3.453 ± 0.458
2.959SerGlu: 2.959 ± 0.421
1.057SerPhe: 1.057 ± 0.231
4.721SerGly: 4.721 ± 0.621
1.127SerHis: 1.127 ± 0.253
2.678SerIle: 2.678 ± 0.363
2.466SerLys: 2.466 ± 0.394
5.214SerLeu: 5.214 ± 0.638
1.268SerMet: 1.268 ± 0.308
2.818SerAsn: 2.818 ± 0.401
2.255SerPro: 2.255 ± 0.434
2.678SerGln: 2.678 ± 0.437
2.678SerArg: 2.678 ± 0.418
3.171SerSer: 3.171 ± 0.567
3.312SerThr: 3.312 ± 0.445
3.805SerVal: 3.805 ± 0.486
0.564SerTrp: 0.564 ± 0.229
1.621SerTyr: 1.621 ± 0.31
0.0SerXaa: 0.0 ± 0.0
Thr
6.412ThrAla: 6.412 ± 0.702
0.352ThrCys: 0.352 ± 0.16
3.03ThrAsp: 3.03 ± 0.369
3.171ThrGlu: 3.171 ± 0.438
2.184ThrPhe: 2.184 ± 0.486
5.144ThrGly: 5.144 ± 0.582
1.48ThrHis: 1.48 ± 0.345
2.396ThrIle: 2.396 ± 0.383
2.466ThrLys: 2.466 ± 0.461
5.919ThrLeu: 5.919 ± 0.674
1.621ThrMet: 1.621 ± 0.302
3.241ThrAsn: 3.241 ± 0.429
3.171ThrPro: 3.171 ± 0.509
2.184ThrGln: 2.184 ± 0.363
2.537ThrArg: 2.537 ± 0.452
3.03ThrSer: 3.03 ± 0.54
3.523ThrThr: 3.523 ± 0.556
4.016ThrVal: 4.016 ± 0.65
0.846ThrTrp: 0.846 ± 0.212
0.916ThrTyr: 0.916 ± 0.243
0.0ThrXaa: 0.0 ± 0.0
Val
5.919ValAla: 5.919 ± 0.728
1.268ValCys: 1.268 ± 0.344
4.157ValAsp: 4.157 ± 0.424
4.651ValGlu: 4.651 ± 0.573
2.325ValPhe: 2.325 ± 0.371
4.228ValGly: 4.228 ± 0.539
1.409ValHis: 1.409 ± 0.373
2.959ValIle: 2.959 ± 0.625
4.369ValLys: 4.369 ± 0.523
5.073ValLeu: 5.073 ± 0.619
1.832ValMet: 1.832 ± 0.275
2.818ValAsn: 2.818 ± 0.482
3.734ValPro: 3.734 ± 0.571
2.396ValGln: 2.396 ± 0.416
3.875ValArg: 3.875 ± 0.46
3.1ValSer: 3.1 ± 0.511
4.439ValThr: 4.439 ± 0.598
5.567ValVal: 5.567 ± 0.744
1.198ValTrp: 1.198 ± 0.307
2.466ValTyr: 2.466 ± 0.448
0.0ValXaa: 0.0 ± 0.0
Trp
1.057TrpAla: 1.057 ± 0.26
0.211TrpCys: 0.211 ± 0.133
1.268TrpAsp: 1.268 ± 0.262
0.846TrpGlu: 0.846 ± 0.207
0.423TrpPhe: 0.423 ± 0.156
0.916TrpGly: 0.916 ± 0.252
0.352TrpHis: 0.352 ± 0.167
0.493TrpIle: 0.493 ± 0.195
1.268TrpLys: 1.268 ± 0.322
1.621TrpLeu: 1.621 ± 0.423
0.775TrpMet: 0.775 ± 0.264
0.564TrpAsn: 0.564 ± 0.188
0.352TrpPro: 0.352 ± 0.124
0.493TrpGln: 0.493 ± 0.174
1.127TrpArg: 1.127 ± 0.286
1.339TrpSer: 1.339 ± 0.312
0.846TrpThr: 0.846 ± 0.201
1.198TrpVal: 1.198 ± 0.307
0.352TrpTrp: 0.352 ± 0.138
0.423TrpTyr: 0.423 ± 0.21
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.537TyrAla: 2.537 ± 0.445
0.564TyrCys: 0.564 ± 0.193
3.382TyrAsp: 3.382 ± 0.554
1.691TyrGlu: 1.691 ± 0.408
0.564TyrPhe: 0.564 ± 0.22
2.889TyrGly: 2.889 ± 0.378
0.846TyrHis: 0.846 ± 0.21
1.762TyrIle: 1.762 ± 0.384
2.184TyrLys: 2.184 ± 0.427
2.255TyrLeu: 2.255 ± 0.453
0.705TyrMet: 0.705 ± 0.195
1.55TyrAsn: 1.55 ± 0.32
1.409TyrPro: 1.409 ± 0.279
1.339TyrGln: 1.339 ± 0.286
2.043TyrArg: 2.043 ± 0.467
1.48TyrSer: 1.48 ± 0.279
1.48TyrThr: 1.48 ± 0.487
2.114TyrVal: 2.114 ± 0.357
0.705TyrTrp: 0.705 ± 0.227
1.339TyrTyr: 1.339 ± 0.36
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 69 proteins (14193 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski