Orientia tsutsugamushi (strain Boryong) (Rickettsia tsutsugamushi)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Rickettsiaceae; Rickettsieae; Orientia; Orientia tsutsugamushi

Average proteome isoelectric point is 7.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 966 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A5CDM5|A5CDM5_ORITB Cytochrome c-type biogenesis protein OS=Orientia tsutsugamushi (strain Boryong) OX=357244 GN=OTBS_0923 PE=3 SV=1
MM1 pKa = 7.07TVAINQYY8 pKa = 10.64INFKK12 pKa = 10.07MEE14 pKa = 4.63TINVTEE20 pKa = 4.43LFMEE24 pKa = 4.28NSDD27 pKa = 4.35IISSTVADD35 pKa = 3.83MTSVVTDD42 pKa = 3.38MTSAVTDD49 pKa = 3.53MTSTVTDD56 pKa = 3.45MTSTVTDD63 pKa = 3.4MTSAVTDD70 pKa = 3.53MTSTVTDD77 pKa = 3.45MTSTVTDD84 pKa = 3.45MTSTVTDD91 pKa = 3.07IGSTISSIISSTINSIISSTTSDD114 pKa = 3.07EE115 pKa = 4.41MIGNNSQSQEE125 pKa = 4.12SEE127 pKa = 4.15GYY129 pKa = 10.17YY130 pKa = 10.23YY131 pKa = 10.86SQLGTWLAVGFTICTLGAVCGLAYY155 pKa = 10.5LYY157 pKa = 10.67KK158 pKa = 10.5KK159 pKa = 8.71RR160 pKa = 11.84TKK162 pKa = 10.08PLEE165 pKa = 4.58DD166 pKa = 3.62KK167 pKa = 10.89SSKK170 pKa = 10.72NYY172 pKa = 9.84EE173 pKa = 4.07LSDD176 pKa = 4.04LNCVKK181 pKa = 10.37VDD183 pKa = 3.73IPLQEE188 pKa = 4.19NEE190 pKa = 4.1IKK192 pKa = 10.61VLSVSDD198 pKa = 3.72VSYY201 pKa = 11.28EE202 pKa = 4.11DD203 pKa = 3.42NGNNDD208 pKa = 2.64SHH210 pKa = 7.99IPSNDD215 pKa = 3.07LDD217 pKa = 4.16NNLLGVNVTEE227 pKa = 4.6HH228 pKa = 6.68LSS230 pKa = 3.33

Molecular weight: 24.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A5CCD5|A5CCD5_ORITB Uncharacterized protein OS=Orientia tsutsugamushi (strain Boryong) OX=357244 GN=OTBS_0266 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.12RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.91GFMEE20 pKa = 4.22RR21 pKa = 11.84MSSVGGRR28 pKa = 11.84RR29 pKa = 11.84VLMRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84MKK37 pKa = 10.42GRR39 pKa = 11.84RR40 pKa = 11.84VLSAA44 pKa = 3.76

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

966

0

966

302339

32

2299

313.0

35.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.053 ± 0.06

1.464 ± 0.035

4.925 ± 0.068

5.382 ± 0.073

4.383 ± 0.058

4.742 ± 0.08

2.13 ± 0.035

10.23 ± 0.086

7.863 ± 0.068

10.177 ± 0.085

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.138 ± 0.032

7.06 ± 0.091

2.842 ± 0.041

4.043 ± 0.051

3.31 ± 0.053

7.527 ± 0.065

5.161 ± 0.053

5.743 ± 0.062

0.759 ± 0.025

4.069 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski