Amino acid dipepetide frequency for Lactobacillus phage Ld25A

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.993AlaAla: 1.993 ± 0.963
0.797AlaCys: 0.797 ± 0.326
4.583AlaAsp: 4.583 ± 0.656
4.384AlaGlu: 4.384 ± 0.74
2.989AlaPhe: 2.989 ± 0.444
6.078AlaGly: 6.078 ± 1.507
0.697AlaHis: 0.697 ± 0.344
5.082AlaIle: 5.082 ± 0.6
5.58AlaLys: 5.58 ± 0.733
4.185AlaLeu: 4.185 ± 0.77
1.395AlaMet: 1.395 ± 0.495
4.285AlaAsn: 4.285 ± 0.672
1.395AlaPro: 1.395 ± 0.388
2.69AlaGln: 2.69 ± 0.716
2.989AlaArg: 2.989 ± 0.658
5.082AlaSer: 5.082 ± 1.169
3.886AlaThr: 3.886 ± 0.688
5.381AlaVal: 5.381 ± 0.761
0.897AlaTrp: 0.897 ± 0.28
3.487AlaTyr: 3.487 ± 0.611
0.0AlaXaa: 0.0 ± 0.0
Cys
0.399CysAla: 0.399 ± 0.2
0.1CysCys: 0.1 ± 0.103
0.299CysAsp: 0.299 ± 0.19
0.697CysGlu: 0.697 ± 0.238
0.199CysPhe: 0.199 ± 0.14
1.096CysGly: 1.096 ± 0.362
0.299CysHis: 0.299 ± 0.18
0.399CysIle: 0.399 ± 0.183
0.996CysLys: 0.996 ± 0.399
0.697CysLeu: 0.697 ± 0.256
0.399CysMet: 0.399 ± 0.177
0.299CysAsn: 0.299 ± 0.162
0.199CysPro: 0.199 ± 0.136
0.399CysGln: 0.399 ± 0.199
0.498CysArg: 0.498 ± 0.231
0.299CysSer: 0.299 ± 0.185
0.498CysThr: 0.498 ± 0.219
0.797CysVal: 0.797 ± 0.369
0.199CysTrp: 0.199 ± 0.143
0.399CysTyr: 0.399 ± 0.194
0.0CysXaa: 0.0 ± 0.0
Asp
2.79AspAla: 2.79 ± 0.5
0.598AspCys: 0.598 ± 0.236
4.583AspAsp: 4.583 ± 0.655
4.982AspGlu: 4.982 ± 0.698
2.989AspPhe: 2.989 ± 0.622
5.181AspGly: 5.181 ± 0.751
0.797AspHis: 0.797 ± 0.273
3.786AspIle: 3.786 ± 0.886
5.281AspLys: 5.281 ± 1.094
5.48AspLeu: 5.48 ± 0.59
1.993AspMet: 1.993 ± 0.498
4.882AspAsn: 4.882 ± 0.704
1.295AspPro: 1.295 ± 0.436
2.292AspGln: 2.292 ± 0.426
1.694AspArg: 1.694 ± 0.404
4.982AspSer: 4.982 ± 0.672
3.487AspThr: 3.487 ± 0.626
4.185AspVal: 4.185 ± 0.541
1.395AspTrp: 1.395 ± 0.48
3.388AspTyr: 3.388 ± 0.676
0.0AspXaa: 0.0 ± 0.0
Glu
4.683GluAla: 4.683 ± 0.543
0.598GluCys: 0.598 ± 0.248
5.48GluAsp: 5.48 ± 0.682
5.978GluGlu: 5.978 ± 0.89
2.092GluPhe: 2.092 ± 0.362
3.189GluGly: 3.189 ± 0.49
0.897GluHis: 0.897 ± 0.257
5.58GluIle: 5.58 ± 0.999
6.078GluLys: 6.078 ± 0.864
5.58GluLeu: 5.58 ± 0.786
2.491GluMet: 2.491 ± 0.507
4.583GluAsn: 4.583 ± 0.704
1.295GluPro: 1.295 ± 0.348
2.491GluGln: 2.491 ± 0.392
2.79GluArg: 2.79 ± 0.596
2.391GluSer: 2.391 ± 0.368
3.487GluThr: 3.487 ± 0.652
5.381GluVal: 5.381 ± 0.716
0.299GluTrp: 0.299 ± 0.17
2.491GluTyr: 2.491 ± 0.491
0.0GluXaa: 0.0 ± 0.0
Phe
1.893PheAla: 1.893 ± 0.442
0.399PheCys: 0.399 ± 0.222
2.391PheAsp: 2.391 ± 0.543
3.089PheGlu: 3.089 ± 0.464
1.594PhePhe: 1.594 ± 0.53
2.69PheGly: 2.69 ± 0.552
0.399PheHis: 0.399 ± 0.198
3.089PheIle: 3.089 ± 0.632
3.886PheLys: 3.886 ± 0.545
3.189PheLeu: 3.189 ± 0.687
0.598PheMet: 0.598 ± 0.279
2.491PheAsn: 2.491 ± 0.569
0.897PhePro: 0.897 ± 0.294
1.495PheGln: 1.495 ± 0.387
1.993PheArg: 1.993 ± 0.508
2.89PheSer: 2.89 ± 0.672
3.487PheThr: 3.487 ± 0.554
2.491PheVal: 2.491 ± 0.386
0.0PheTrp: 0.0 ± 0.0
0.598PheTyr: 0.598 ± 0.21
0.0PheXaa: 0.0 ± 0.0
Gly
4.783GlyAla: 4.783 ± 0.857
0.199GlyCys: 0.199 ± 0.131
5.978GlyAsp: 5.978 ± 1.363
2.89GlyGlu: 2.89 ± 0.514
3.687GlyPhe: 3.687 ± 0.709
4.185GlyGly: 4.185 ± 0.716
0.996GlyHis: 0.996 ± 0.273
5.978GlyIle: 5.978 ± 0.912
6.277GlyLys: 6.277 ± 0.865
6.277GlyLeu: 6.277 ± 0.773
1.495GlyMet: 1.495 ± 0.458
4.384GlyAsn: 4.384 ± 0.874
0.1GlyPro: 0.1 ± 0.11
1.893GlyGln: 1.893 ± 0.317
3.288GlyArg: 3.288 ± 0.516
3.986GlySer: 3.986 ± 0.903
3.786GlyThr: 3.786 ± 0.653
5.68GlyVal: 5.68 ± 0.751
0.797GlyTrp: 0.797 ± 0.205
2.69GlyTyr: 2.69 ± 0.562
0.0GlyXaa: 0.0 ± 0.0
His
1.196HisAla: 1.196 ± 0.369
0.199HisCys: 0.199 ± 0.141
0.598HisAsp: 0.598 ± 0.221
0.697HisGlu: 0.697 ± 0.321
0.299HisPhe: 0.299 ± 0.161
0.797HisGly: 0.797 ± 0.269
0.598HisHis: 0.598 ± 0.405
0.996HisIle: 0.996 ± 0.305
1.395HisLys: 1.395 ± 0.383
0.697HisLeu: 0.697 ± 0.283
0.299HisMet: 0.299 ± 0.149
0.498HisAsn: 0.498 ± 0.19
0.299HisPro: 0.299 ± 0.166
0.299HisGln: 0.299 ± 0.157
0.697HisArg: 0.697 ± 0.279
0.996HisSer: 0.996 ± 0.238
0.797HisThr: 0.797 ± 0.308
1.495HisVal: 1.495 ± 0.438
0.0HisTrp: 0.0 ± 0.0
0.797HisTyr: 0.797 ± 0.282
0.0HisXaa: 0.0 ± 0.0
Ile
3.886IleAla: 3.886 ± 0.605
0.797IleCys: 0.797 ± 0.304
4.882IleAsp: 4.882 ± 0.687
5.68IleGlu: 5.68 ± 0.782
1.694IlePhe: 1.694 ± 0.37
3.687IleGly: 3.687 ± 0.636
0.897IleHis: 0.897 ± 0.254
4.185IleIle: 4.185 ± 0.529
7.174IleLys: 7.174 ± 0.879
4.484IleLeu: 4.484 ± 0.874
1.893IleMet: 1.893 ± 0.455
5.68IleAsn: 5.68 ± 0.543
2.591IlePro: 2.591 ± 0.506
1.794IleGln: 1.794 ± 0.345
2.591IleArg: 2.591 ± 0.566
4.882IleSer: 4.882 ± 0.627
4.783IleThr: 4.783 ± 0.584
3.786IleVal: 3.786 ± 0.628
0.697IleTrp: 0.697 ± 0.295
2.79IleTyr: 2.79 ± 0.468
0.0IleXaa: 0.0 ± 0.0
Lys
7.174LysAla: 7.174 ± 0.899
0.1LysCys: 0.1 ± 0.105
3.886LysAsp: 3.886 ± 0.574
5.879LysGlu: 5.879 ± 0.803
2.89LysPhe: 2.89 ± 0.463
7.473LysGly: 7.473 ± 1.528
1.096LysHis: 1.096 ± 0.314
5.978LysIle: 5.978 ± 0.854
7.274LysLys: 7.274 ± 1.092
7.573LysLeu: 7.573 ± 0.774
1.794LysMet: 1.794 ± 0.402
6.178LysAsn: 6.178 ± 1.006
1.794LysPro: 1.794 ± 0.361
3.487LysGln: 3.487 ± 0.662
3.886LysArg: 3.886 ± 0.645
4.484LysSer: 4.484 ± 0.58
5.281LysThr: 5.281 ± 0.735
5.58LysVal: 5.58 ± 0.866
0.697LysTrp: 0.697 ± 0.275
3.886LysTyr: 3.886 ± 0.62
0.0LysXaa: 0.0 ± 0.0
Leu
6.975LeuAla: 6.975 ± 0.993
1.096LeuCys: 1.096 ± 0.383
4.982LeuAsp: 4.982 ± 0.841
4.982LeuGlu: 4.982 ± 0.711
2.69LeuPhe: 2.69 ± 0.647
4.285LeuGly: 4.285 ± 0.887
1.196LeuHis: 1.196 ± 0.419
4.384LeuIle: 4.384 ± 0.719
7.573LeuLys: 7.573 ± 0.808
6.676LeuLeu: 6.676 ± 1.074
1.495LeuMet: 1.495 ± 0.319
4.484LeuAsn: 4.484 ± 0.698
2.89LeuPro: 2.89 ± 0.604
1.794LeuGln: 1.794 ± 0.293
3.189LeuArg: 3.189 ± 0.601
5.58LeuSer: 5.58 ± 0.724
4.982LeuThr: 4.982 ± 0.514
4.583LeuVal: 4.583 ± 0.537
0.399LeuTrp: 0.399 ± 0.199
2.391LeuTyr: 2.391 ± 0.579
0.0LeuXaa: 0.0 ± 0.0
Met
2.491MetAla: 2.491 ± 0.557
0.199MetCys: 0.199 ± 0.14
1.495MetAsp: 1.495 ± 0.393
1.893MetGlu: 1.893 ± 0.46
0.598MetPhe: 0.598 ± 0.227
0.996MetGly: 0.996 ± 0.325
0.199MetHis: 0.199 ± 0.133
1.694MetIle: 1.694 ± 0.377
2.989MetLys: 2.989 ± 0.599
1.196MetLeu: 1.196 ± 0.356
0.697MetMet: 0.697 ± 0.284
1.594MetAsn: 1.594 ± 0.358
0.399MetPro: 0.399 ± 0.169
0.498MetGln: 0.498 ± 0.188
1.495MetArg: 1.495 ± 0.375
1.794MetSer: 1.794 ± 0.531
1.295MetThr: 1.295 ± 0.348
1.495MetVal: 1.495 ± 0.41
0.399MetTrp: 0.399 ± 0.177
0.199MetTyr: 0.199 ± 0.155
0.0MetXaa: 0.0 ± 0.0
Asn
5.181AsnAla: 5.181 ± 1.032
0.996AsnCys: 0.996 ± 0.337
3.189AsnAsp: 3.189 ± 0.637
6.178AsnGlu: 6.178 ± 0.883
2.79AsnPhe: 2.79 ± 0.519
7.672AsnGly: 7.672 ± 1.096
0.897AsnHis: 0.897 ± 0.347
4.085AsnIle: 4.085 ± 0.572
4.583AsnLys: 4.583 ± 0.654
4.783AsnLeu: 4.783 ± 0.632
1.495AsnMet: 1.495 ± 0.331
3.687AsnAsn: 3.687 ± 0.868
1.794AsnPro: 1.794 ± 0.421
1.893AsnGln: 1.893 ± 0.508
2.491AsnArg: 2.491 ± 0.395
5.381AsnSer: 5.381 ± 0.791
2.89AsnThr: 2.89 ± 0.612
2.89AsnVal: 2.89 ± 0.616
0.897AsnTrp: 0.897 ± 0.326
2.491AsnTyr: 2.491 ± 0.495
0.0AsnXaa: 0.0 ± 0.0
Pro
1.794ProAla: 1.794 ± 0.413
0.1ProCys: 0.1 ± 0.103
1.395ProAsp: 1.395 ± 0.365
0.996ProGlu: 0.996 ± 0.315
0.996ProPhe: 0.996 ± 0.277
1.096ProGly: 1.096 ± 0.474
0.299ProHis: 0.299 ± 0.208
1.694ProIle: 1.694 ± 0.44
2.89ProLys: 2.89 ± 0.528
1.495ProLeu: 1.495 ± 0.429
0.299ProMet: 0.299 ± 0.172
1.694ProAsn: 1.694 ± 0.367
0.498ProPro: 0.498 ± 0.187
0.697ProGln: 0.697 ± 0.237
0.897ProArg: 0.897 ± 0.297
1.993ProSer: 1.993 ± 0.479
1.395ProThr: 1.395 ± 0.343
2.591ProVal: 2.591 ± 0.571
0.598ProTrp: 0.598 ± 0.272
1.096ProTyr: 1.096 ± 0.326
0.0ProXaa: 0.0 ± 0.0
Gln
2.69GlnAla: 2.69 ± 0.464
0.299GlnCys: 0.299 ± 0.217
2.192GlnAsp: 2.192 ± 0.361
1.395GlnGlu: 1.395 ± 0.377
1.196GlnPhe: 1.196 ± 0.3
2.391GlnGly: 2.391 ± 0.544
0.598GlnHis: 0.598 ± 0.229
3.089GlnIle: 3.089 ± 0.587
2.391GlnLys: 2.391 ± 0.56
3.089GlnLeu: 3.089 ± 0.927
0.697GlnMet: 0.697 ± 0.326
2.092GlnAsn: 2.092 ± 0.502
0.897GlnPro: 0.897 ± 0.317
1.594GlnGln: 1.594 ± 0.337
0.697GlnArg: 0.697 ± 0.245
2.192GlnSer: 2.192 ± 0.599
1.594GlnThr: 1.594 ± 0.444
2.491GlnVal: 2.491 ± 0.53
0.1GlnTrp: 0.1 ± 0.089
1.594GlnTyr: 1.594 ± 0.373
0.0GlnXaa: 0.0 ± 0.0
Arg
2.989ArgAla: 2.989 ± 0.519
0.697ArgCys: 0.697 ± 0.27
2.491ArgAsp: 2.491 ± 0.463
2.69ArgGlu: 2.69 ± 0.611
2.092ArgPhe: 2.092 ± 0.367
2.092ArgGly: 2.092 ± 0.456
0.498ArgHis: 0.498 ± 0.26
2.79ArgIle: 2.79 ± 0.454
3.786ArgLys: 3.786 ± 0.669
3.388ArgLeu: 3.388 ± 0.446
1.096ArgMet: 1.096 ± 0.323
2.79ArgAsn: 2.79 ± 0.63
0.797ArgPro: 0.797 ± 0.314
1.295ArgGln: 1.295 ± 0.263
1.694ArgArg: 1.694 ± 0.465
1.395ArgSer: 1.395 ± 0.464
2.192ArgThr: 2.192 ± 0.425
2.192ArgVal: 2.192 ± 0.371
0.797ArgTrp: 0.797 ± 0.264
2.391ArgTyr: 2.391 ± 0.397
0.0ArgXaa: 0.0 ± 0.0
Ser
4.384SerAla: 4.384 ± 0.73
0.598SerCys: 0.598 ± 0.235
4.185SerAsp: 4.185 ± 0.616
4.285SerGlu: 4.285 ± 0.631
3.687SerPhe: 3.687 ± 0.528
4.982SerGly: 4.982 ± 0.874
0.797SerHis: 0.797 ± 0.258
3.587SerIle: 3.587 ± 0.506
5.381SerLys: 5.381 ± 0.649
3.886SerLeu: 3.886 ± 0.557
1.893SerMet: 1.893 ± 0.457
4.583SerAsn: 4.583 ± 0.711
1.794SerPro: 1.794 ± 0.408
2.491SerGln: 2.491 ± 0.547
2.192SerArg: 2.192 ± 0.595
5.381SerSer: 5.381 ± 0.885
3.786SerThr: 3.786 ± 0.577
5.181SerVal: 5.181 ± 0.76
0.797SerTrp: 0.797 ± 0.27
2.69SerTyr: 2.69 ± 0.557
0.0SerXaa: 0.0 ± 0.0
Thr
4.185ThrAla: 4.185 ± 0.823
0.399ThrCys: 0.399 ± 0.197
3.587ThrAsp: 3.587 ± 0.55
3.986ThrGlu: 3.986 ± 0.656
3.089ThrPhe: 3.089 ± 0.493
3.886ThrGly: 3.886 ± 0.645
0.399ThrHis: 0.399 ± 0.201
4.384ThrIle: 4.384 ± 0.57
4.583ThrLys: 4.583 ± 0.619
4.683ThrLeu: 4.683 ± 0.689
0.299ThrMet: 0.299 ± 0.17
3.786ThrAsn: 3.786 ± 0.944
2.092ThrPro: 2.092 ± 0.454
1.794ThrGln: 1.794 ± 0.561
1.893ThrArg: 1.893 ± 0.422
3.687ThrSer: 3.687 ± 0.627
3.288ThrThr: 3.288 ± 0.58
5.281ThrVal: 5.281 ± 0.717
0.697ThrTrp: 0.697 ± 0.261
2.491ThrTyr: 2.491 ± 0.607
0.0ThrXaa: 0.0 ± 0.0
Val
6.477ValAla: 6.477 ± 1.049
0.399ValCys: 0.399 ± 0.242
4.982ValAsp: 4.982 ± 0.722
4.583ValGlu: 4.583 ± 0.759
1.594ValPhe: 1.594 ± 0.371
4.683ValGly: 4.683 ± 0.823
1.196ValHis: 1.196 ± 0.281
4.384ValIle: 4.384 ± 0.485
5.779ValLys: 5.779 ± 0.833
4.484ValLeu: 4.484 ± 0.687
1.794ValMet: 1.794 ± 0.328
5.082ValAsn: 5.082 ± 0.683
1.794ValPro: 1.794 ± 0.553
1.893ValGln: 1.893 ± 0.475
2.292ValArg: 2.292 ± 0.394
5.281ValSer: 5.281 ± 0.52
4.285ValThr: 4.285 ± 0.691
4.683ValVal: 4.683 ± 0.721
0.598ValTrp: 0.598 ± 0.288
3.587ValTyr: 3.587 ± 0.657
0.0ValXaa: 0.0 ± 0.0
Trp
0.498TrpAla: 0.498 ± 0.283
0.0TrpCys: 0.0 ± 0.0
0.498TrpAsp: 0.498 ± 0.25
0.697TrpGlu: 0.697 ± 0.236
1.096TrpPhe: 1.096 ± 0.328
0.697TrpGly: 0.697 ± 0.285
0.399TrpHis: 0.399 ± 0.194
0.598TrpIle: 0.598 ± 0.233
0.498TrpLys: 0.498 ± 0.192
1.594TrpLeu: 1.594 ± 0.324
0.0TrpMet: 0.0 ± 0.0
0.897TrpAsn: 0.897 ± 0.379
0.0TrpPro: 0.0 ± 0.0
0.498TrpGln: 0.498 ± 0.19
0.498TrpArg: 0.498 ± 0.217
0.299TrpSer: 0.299 ± 0.174
0.697TrpThr: 0.697 ± 0.25
0.598TrpVal: 0.598 ± 0.261
0.0TrpTrp: 0.0 ± 0.0
0.399TrpTyr: 0.399 ± 0.221
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.395TyrAla: 1.395 ± 0.343
0.598TyrCys: 0.598 ± 0.298
4.384TyrAsp: 4.384 ± 0.563
1.893TyrGlu: 1.893 ± 0.426
1.295TyrPhe: 1.295 ± 0.368
2.292TyrGly: 2.292 ± 0.57
0.498TyrHis: 0.498 ± 0.208
3.089TyrIle: 3.089 ± 0.542
1.993TyrLys: 1.993 ± 0.44
3.189TyrLeu: 3.189 ± 0.566
1.395TyrMet: 1.395 ± 0.363
2.292TyrAsn: 2.292 ± 0.367
1.694TyrPro: 1.694 ± 0.373
1.993TyrGln: 1.993 ± 0.384
2.391TyrArg: 2.391 ± 0.56
3.587TyrSer: 3.587 ± 0.641
2.591TyrThr: 2.591 ± 0.563
3.089TyrVal: 3.089 ± 0.485
0.199TyrTrp: 0.199 ± 0.138
1.594TyrTyr: 1.594 ± 0.481
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 51 proteins (10037 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski