Amino acid dipepetide frequency for Mycobacterium phage Arissanae

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.165AlaAla: 12.165 ± 1.346
0.482AlaCys: 0.482 ± 0.165
5.179AlaAsp: 5.179 ± 0.599
7.347AlaGlu: 7.347 ± 0.786
4.336AlaPhe: 4.336 ± 0.637
7.949AlaGly: 7.949 ± 0.696
1.746AlaHis: 1.746 ± 0.3
4.517AlaIle: 4.517 ± 0.491
5.661AlaLys: 5.661 ± 0.606
9.455AlaLeu: 9.455 ± 0.838
3.312AlaMet: 3.312 ± 0.369
3.312AlaAsn: 3.312 ± 0.519
5.059AlaPro: 5.059 ± 0.598
3.914AlaGln: 3.914 ± 0.524
6.203AlaArg: 6.203 ± 0.825
4.878AlaSer: 4.878 ± 0.531
6.143AlaThr: 6.143 ± 0.735
7.167AlaVal: 7.167 ± 0.721
1.566AlaTrp: 1.566 ± 0.295
2.288AlaTyr: 2.288 ± 0.422
0.0AlaXaa: 0.0 ± 0.0
Cys
0.542CysAla: 0.542 ± 0.174
0.0CysCys: 0.0 ± 0.0
0.542CysAsp: 0.542 ± 0.179
0.361CysGlu: 0.361 ± 0.147
0.241CysPhe: 0.241 ± 0.105
0.542CysGly: 0.542 ± 0.184
0.301CysHis: 0.301 ± 0.14
0.361CysIle: 0.361 ± 0.173
0.181CysLys: 0.181 ± 0.113
0.422CysLeu: 0.422 ± 0.173
0.301CysMet: 0.301 ± 0.125
0.482CysAsn: 0.482 ± 0.163
0.602CysPro: 0.602 ± 0.232
0.241CysGln: 0.241 ± 0.123
0.542CysArg: 0.542 ± 0.18
0.662CysSer: 0.662 ± 0.214
0.542CysThr: 0.542 ± 0.181
0.602CysVal: 0.602 ± 0.194
0.361CysTrp: 0.361 ± 0.139
0.241CysTyr: 0.241 ± 0.114
0.0CysXaa: 0.0 ± 0.0
Asp
6.203AspAla: 6.203 ± 0.614
0.903AspCys: 0.903 ± 0.228
3.071AspAsp: 3.071 ± 0.422
3.975AspGlu: 3.975 ± 0.687
2.529AspPhe: 2.529 ± 0.408
4.878AspGly: 4.878 ± 0.513
1.084AspHis: 1.084 ± 0.256
3.192AspIle: 3.192 ± 0.427
2.228AspLys: 2.228 ± 0.405
6.263AspLeu: 6.263 ± 0.702
1.506AspMet: 1.506 ± 0.347
1.927AspAsn: 1.927 ± 0.334
3.734AspPro: 3.734 ± 0.7
1.686AspGln: 1.686 ± 0.287
3.312AspArg: 3.312 ± 0.487
3.734AspSer: 3.734 ± 0.383
3.734AspThr: 3.734 ± 0.437
4.396AspVal: 4.396 ± 0.557
1.325AspTrp: 1.325 ± 0.352
2.891AspTyr: 2.891 ± 0.438
0.0AspXaa: 0.0 ± 0.0
Glu
6.263GluAla: 6.263 ± 0.653
0.181GluCys: 0.181 ± 0.091
4.155GluAsp: 4.155 ± 0.601
5.239GluGlu: 5.239 ± 0.674
2.228GluPhe: 2.228 ± 0.33
5.661GluGly: 5.661 ± 0.707
1.867GluHis: 1.867 ± 0.316
3.734GluIle: 3.734 ± 0.47
2.71GluLys: 2.71 ± 0.439
6.203GluLeu: 6.203 ± 0.586
2.409GluMet: 2.409 ± 0.373
1.927GluAsn: 1.927 ± 0.307
2.951GluPro: 2.951 ± 0.452
2.409GluGln: 2.409 ± 0.311
4.697GluArg: 4.697 ± 0.65
2.951GluSer: 2.951 ± 0.466
3.372GluThr: 3.372 ± 0.423
4.396GluVal: 4.396 ± 0.439
1.506GluTrp: 1.506 ± 0.316
1.867GluTyr: 1.867 ± 0.281
0.0GluXaa: 0.0 ± 0.0
Phe
3.192PheAla: 3.192 ± 0.508
0.361PheCys: 0.361 ± 0.145
2.83PheAsp: 2.83 ± 0.542
2.65PheGlu: 2.65 ± 0.513
0.783PhePhe: 0.783 ± 0.253
3.192PheGly: 3.192 ± 0.34
0.723PheHis: 0.723 ± 0.194
1.686PheIle: 1.686 ± 0.318
1.084PheLys: 1.084 ± 0.304
2.349PheLeu: 2.349 ± 0.468
0.783PheMet: 0.783 ± 0.19
1.987PheAsn: 1.987 ± 0.346
1.566PhePro: 1.566 ± 0.281
1.024PheGln: 1.024 ± 0.2
2.048PheArg: 2.048 ± 0.331
2.168PheSer: 2.168 ± 0.351
2.288PheThr: 2.288 ± 0.375
2.108PheVal: 2.108 ± 0.376
0.662PheTrp: 0.662 ± 0.225
0.903PheTyr: 0.903 ± 0.269
0.0PheXaa: 0.0 ± 0.0
Gly
7.106GlyAla: 7.106 ± 0.976
0.783GlyCys: 0.783 ± 0.227
5.781GlyAsp: 5.781 ± 0.523
4.758GlyGlu: 4.758 ± 0.548
3.071GlyPhe: 3.071 ± 0.427
8.672GlyGly: 8.672 ± 1.247
1.686GlyHis: 1.686 ± 0.315
4.456GlyIle: 4.456 ± 0.646
3.553GlyLys: 3.553 ± 0.467
6.564GlyLeu: 6.564 ± 0.707
2.77GlyMet: 2.77 ± 0.496
3.011GlyAsn: 3.011 ± 0.433
3.674GlyPro: 3.674 ± 0.38
3.011GlyGln: 3.011 ± 0.596
4.035GlyArg: 4.035 ± 0.473
4.396GlySer: 4.396 ± 0.579
5.239GlyThr: 5.239 ± 0.583
6.384GlyVal: 6.384 ± 0.67
1.927GlyTrp: 1.927 ± 0.301
2.529GlyTyr: 2.529 ± 0.441
0.0GlyXaa: 0.0 ± 0.0
His
1.325HisAla: 1.325 ± 0.3
0.181HisCys: 0.181 ± 0.108
1.204HisAsp: 1.204 ± 0.313
0.964HisGlu: 0.964 ± 0.238
0.542HisPhe: 0.542 ± 0.192
1.445HisGly: 1.445 ± 0.346
0.602HisHis: 0.602 ± 0.162
0.662HisIle: 0.662 ± 0.194
0.964HisLys: 0.964 ± 0.27
1.024HisLeu: 1.024 ± 0.311
0.361HisMet: 0.361 ± 0.159
0.542HisAsn: 0.542 ± 0.179
1.204HisPro: 1.204 ± 0.275
0.783HisGln: 0.783 ± 0.224
1.265HisArg: 1.265 ± 0.317
0.964HisSer: 0.964 ± 0.235
1.144HisThr: 1.144 ± 0.275
1.686HisVal: 1.686 ± 0.305
0.361HisTrp: 0.361 ± 0.15
0.783HisTyr: 0.783 ± 0.282
0.0HisXaa: 0.0 ± 0.0
Ile
4.035IleAla: 4.035 ± 0.445
0.602IleCys: 0.602 ± 0.176
4.216IleAsp: 4.216 ± 0.461
4.276IleGlu: 4.276 ± 0.619
1.566IlePhe: 1.566 ± 0.307
3.914IleGly: 3.914 ± 0.517
0.903IleHis: 0.903 ± 0.233
1.626IleIle: 1.626 ± 0.289
2.59IleLys: 2.59 ± 0.406
3.553IleLeu: 3.553 ± 0.423
0.903IleMet: 0.903 ± 0.233
2.168IleAsn: 2.168 ± 0.388
3.312IlePro: 3.312 ± 0.394
1.686IleGln: 1.686 ± 0.3
3.493IleArg: 3.493 ± 0.448
2.891IleSer: 2.891 ± 0.422
3.613IleThr: 3.613 ± 0.536
3.553IleVal: 3.553 ± 0.476
0.662IleTrp: 0.662 ± 0.214
1.144IleTyr: 1.144 ± 0.255
0.0IleXaa: 0.0 ± 0.0
Lys
4.637LysAla: 4.637 ± 0.525
0.181LysCys: 0.181 ± 0.1
2.168LysAsp: 2.168 ± 0.306
2.288LysGlu: 2.288 ± 0.373
1.084LysPhe: 1.084 ± 0.222
3.433LysGly: 3.433 ± 0.456
0.542LysHis: 0.542 ± 0.192
2.288LysIle: 2.288 ± 0.365
2.168LysLys: 2.168 ± 0.423
4.216LysLeu: 4.216 ± 0.489
1.265LysMet: 1.265 ± 0.271
1.024LysAsn: 1.024 ± 0.273
2.409LysPro: 2.409 ± 0.486
1.566LysGln: 1.566 ± 0.265
4.095LysArg: 4.095 ± 0.711
2.349LysSer: 2.349 ± 0.337
2.168LysThr: 2.168 ± 0.414
3.372LysVal: 3.372 ± 0.499
0.843LysTrp: 0.843 ± 0.272
1.385LysTyr: 1.385 ± 0.27
0.0LysXaa: 0.0 ± 0.0
Leu
10.117LeuAla: 10.117 ± 0.876
1.024LeuCys: 1.024 ± 0.25
4.637LeuAsp: 4.637 ± 0.519
5.059LeuGlu: 5.059 ± 0.515
2.409LeuPhe: 2.409 ± 0.36
6.203LeuGly: 6.203 ± 0.638
1.024LeuHis: 1.024 ± 0.313
4.336LeuIle: 4.336 ± 0.51
2.891LeuLys: 2.891 ± 0.371
5.781LeuLeu: 5.781 ± 0.607
2.288LeuMet: 2.288 ± 0.39
3.132LeuAsn: 3.132 ± 0.496
4.818LeuPro: 4.818 ± 0.667
2.469LeuGln: 2.469 ± 0.521
5.781LeuArg: 5.781 ± 0.576
6.022LeuSer: 6.022 ± 0.575
5.842LeuThr: 5.842 ± 0.508
4.517LeuVal: 4.517 ± 0.463
1.686LeuTrp: 1.686 ± 0.269
1.746LeuTyr: 1.746 ± 0.367
0.0LeuXaa: 0.0 ± 0.0
Met
3.312MetAla: 3.312 ± 0.449
0.12MetCys: 0.12 ± 0.083
1.084MetAsp: 1.084 ± 0.214
2.288MetGlu: 2.288 ± 0.369
0.482MetPhe: 0.482 ± 0.171
2.048MetGly: 2.048 ± 0.375
0.181MetHis: 0.181 ± 0.098
1.506MetIle: 1.506 ± 0.299
1.445MetLys: 1.445 ± 0.317
1.445MetLeu: 1.445 ± 0.342
0.542MetMet: 0.542 ± 0.221
0.723MetAsn: 0.723 ± 0.201
1.445MetPro: 1.445 ± 0.246
0.903MetGln: 0.903 ± 0.208
1.867MetArg: 1.867 ± 0.358
2.168MetSer: 2.168 ± 0.325
2.59MetThr: 2.59 ± 0.34
1.265MetVal: 1.265 ± 0.315
0.422MetTrp: 0.422 ± 0.133
0.602MetTyr: 0.602 ± 0.183
0.0MetXaa: 0.0 ± 0.0
Asn
3.493AsnAla: 3.493 ± 0.399
0.181AsnCys: 0.181 ± 0.11
1.686AsnAsp: 1.686 ± 0.343
2.048AsnGlu: 2.048 ± 0.337
1.204AsnPhe: 1.204 ± 0.317
3.553AsnGly: 3.553 ± 0.531
1.144AsnHis: 1.144 ± 0.269
1.686AsnIle: 1.686 ± 0.317
0.903AsnLys: 0.903 ± 0.233
2.83AsnLeu: 2.83 ± 0.401
0.783AsnMet: 0.783 ± 0.163
0.662AsnAsn: 0.662 ± 0.183
2.77AsnPro: 2.77 ± 0.382
0.783AsnGln: 0.783 ± 0.181
2.409AsnArg: 2.409 ± 0.446
2.228AsnSer: 2.228 ± 0.376
2.288AsnThr: 2.288 ± 0.392
2.59AsnVal: 2.59 ± 0.385
0.602AsnTrp: 0.602 ± 0.192
0.903AsnTyr: 0.903 ± 0.183
0.0AsnXaa: 0.0 ± 0.0
Pro
5.661ProAla: 5.661 ± 0.656
0.301ProCys: 0.301 ± 0.136
3.312ProAsp: 3.312 ± 0.449
4.456ProGlu: 4.456 ± 0.631
1.927ProPhe: 1.927 ± 0.345
4.697ProGly: 4.697 ± 0.477
0.964ProHis: 0.964 ± 0.291
2.83ProIle: 2.83 ± 0.376
1.867ProLys: 1.867 ± 0.337
3.252ProLeu: 3.252 ± 0.433
1.265ProMet: 1.265 ± 0.282
2.168ProAsn: 2.168 ± 0.404
1.626ProPro: 1.626 ± 0.358
1.686ProGln: 1.686 ± 0.376
2.891ProArg: 2.891 ± 0.445
3.011ProSer: 3.011 ± 0.374
3.734ProThr: 3.734 ± 0.437
4.095ProVal: 4.095 ± 0.493
0.602ProTrp: 0.602 ± 0.209
1.746ProTyr: 1.746 ± 0.298
0.0ProXaa: 0.0 ± 0.0
Gln
5.059GlnAla: 5.059 ± 0.719
0.12GlnCys: 0.12 ± 0.076
1.385GlnAsp: 1.385 ± 0.231
1.204GlnGlu: 1.204 ± 0.25
1.084GlnPhe: 1.084 ± 0.223
3.132GlnGly: 3.132 ± 0.449
0.602GlnHis: 0.602 ± 0.141
1.927GlnIle: 1.927 ± 0.291
1.385GlnLys: 1.385 ± 0.396
2.83GlnLeu: 2.83 ± 0.608
0.903GlnMet: 0.903 ± 0.273
0.843GlnAsn: 0.843 ± 0.194
1.566GlnPro: 1.566 ± 0.291
1.746GlnGln: 1.746 ± 0.399
2.77GlnArg: 2.77 ± 0.357
2.288GlnSer: 2.288 ± 0.477
2.349GlnThr: 2.349 ± 0.38
2.168GlnVal: 2.168 ± 0.303
0.903GlnTrp: 0.903 ± 0.235
1.024GlnTyr: 1.024 ± 0.246
0.0GlnXaa: 0.0 ± 0.0
Arg
5.601ArgAla: 5.601 ± 0.595
1.024ArgCys: 1.024 ± 0.264
3.914ArgAsp: 3.914 ± 0.551
4.697ArgGlu: 4.697 ± 0.594
2.71ArgPhe: 2.71 ± 0.391
4.276ArgGly: 4.276 ± 0.556
1.024ArgHis: 1.024 ± 0.275
3.914ArgIle: 3.914 ± 0.52
3.071ArgLys: 3.071 ± 0.474
5.721ArgLeu: 5.721 ± 0.532
1.867ArgMet: 1.867 ± 0.351
2.529ArgAsn: 2.529 ± 0.39
2.77ArgPro: 2.77 ± 0.447
2.83ArgGln: 2.83 ± 0.371
5.239ArgArg: 5.239 ± 0.775
3.252ArgSer: 3.252 ± 0.457
3.252ArgThr: 3.252 ± 0.411
3.794ArgVal: 3.794 ± 0.514
1.325ArgTrp: 1.325 ± 0.261
1.686ArgTyr: 1.686 ± 0.331
0.0ArgXaa: 0.0 ± 0.0
Ser
6.143SerAla: 6.143 ± 0.501
0.301SerCys: 0.301 ± 0.124
3.914SerAsp: 3.914 ± 0.572
3.975SerGlu: 3.975 ± 0.505
2.168SerPhe: 2.168 ± 0.423
5.54SerGly: 5.54 ± 0.668
0.783SerHis: 0.783 ± 0.2
2.349SerIle: 2.349 ± 0.368
2.529SerLys: 2.529 ± 0.372
4.216SerLeu: 4.216 ± 0.59
1.807SerMet: 1.807 ± 0.294
1.746SerAsn: 1.746 ± 0.315
2.83SerPro: 2.83 ± 0.361
1.987SerGln: 1.987 ± 0.329
3.854SerArg: 3.854 ± 0.457
2.83SerSer: 2.83 ± 0.52
2.83SerThr: 2.83 ± 0.46
3.854SerVal: 3.854 ± 0.453
1.445SerTrp: 1.445 ± 0.338
1.385SerTyr: 1.385 ± 0.287
0.0SerXaa: 0.0 ± 0.0
Thr
6.564ThrAla: 6.564 ± 0.674
0.361ThrCys: 0.361 ± 0.132
4.396ThrAsp: 4.396 ± 0.62
3.192ThrGlu: 3.192 ± 0.438
2.409ThrPhe: 2.409 ± 0.482
5.781ThrGly: 5.781 ± 0.754
1.204ThrHis: 1.204 ± 0.267
2.891ThrIle: 2.891 ± 0.398
2.83ThrLys: 2.83 ± 0.435
5.48ThrLeu: 5.48 ± 0.506
1.144ThrMet: 1.144 ± 0.283
1.686ThrAsn: 1.686 ± 0.303
4.155ThrPro: 4.155 ± 0.436
2.108ThrGln: 2.108 ± 0.335
2.529ThrArg: 2.529 ± 0.334
2.77ThrSer: 2.77 ± 0.443
3.433ThrThr: 3.433 ± 0.458
5.48ThrVal: 5.48 ± 0.632
0.964ThrTrp: 0.964 ± 0.27
2.168ThrTyr: 2.168 ± 0.328
0.0ThrXaa: 0.0 ± 0.0
Val
6.685ValAla: 6.685 ± 0.606
0.542ValCys: 0.542 ± 0.165
5.842ValAsp: 5.842 ± 0.778
4.396ValGlu: 4.396 ± 0.527
2.288ValPhe: 2.288 ± 0.428
5.059ValGly: 5.059 ± 0.582
1.084ValHis: 1.084 ± 0.331
4.035ValIle: 4.035 ± 0.487
4.035ValLys: 4.035 ± 0.432
5.54ValLeu: 5.54 ± 0.71
0.964ValMet: 0.964 ± 0.267
3.252ValAsn: 3.252 ± 0.501
3.493ValPro: 3.493 ± 0.501
2.168ValGln: 2.168 ± 0.344
4.517ValArg: 4.517 ± 0.396
4.577ValSer: 4.577 ± 0.496
4.095ValThr: 4.095 ± 0.472
4.697ValVal: 4.697 ± 0.54
1.144ValTrp: 1.144 ± 0.221
1.566ValTyr: 1.566 ± 0.305
0.0ValXaa: 0.0 ± 0.0
Trp
1.686TrpAla: 1.686 ± 0.362
0.301TrpCys: 0.301 ± 0.144
1.506TrpAsp: 1.506 ± 0.263
1.445TrpGlu: 1.445 ± 0.292
0.422TrpPhe: 0.422 ± 0.165
1.265TrpGly: 1.265 ± 0.339
0.361TrpHis: 0.361 ± 0.167
1.566TrpIle: 1.566 ± 0.278
0.662TrpLys: 0.662 ± 0.203
1.265TrpLeu: 1.265 ± 0.28
0.783TrpMet: 0.783 ± 0.2
0.602TrpAsn: 0.602 ± 0.239
0.783TrpPro: 0.783 ± 0.201
1.265TrpGln: 1.265 ± 0.257
0.903TrpArg: 0.903 ± 0.232
1.084TrpSer: 1.084 ± 0.266
1.144TrpThr: 1.144 ± 0.25
1.265TrpVal: 1.265 ± 0.259
0.482TrpTrp: 0.482 ± 0.19
0.361TrpTyr: 0.361 ± 0.149
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.891TyrAla: 2.891 ± 0.434
0.12TyrCys: 0.12 ± 0.089
1.746TyrAsp: 1.746 ± 0.361
2.108TyrGlu: 2.108 ± 0.387
0.903TyrPhe: 0.903 ± 0.243
2.168TyrGly: 2.168 ± 0.303
0.181TyrHis: 0.181 ± 0.116
1.144TyrIle: 1.144 ± 0.262
0.723TyrLys: 0.723 ± 0.209
3.433TyrLeu: 3.433 ± 0.402
0.422TyrMet: 0.422 ± 0.195
1.024TyrAsn: 1.024 ± 0.278
1.325TyrPro: 1.325 ± 0.257
1.024TyrGln: 1.024 ± 0.249
2.048TyrArg: 2.048 ± 0.376
1.265TyrSer: 1.265 ± 0.284
1.746TyrThr: 1.746 ± 0.309
2.59TyrVal: 2.59 ± 0.444
0.361TyrTrp: 0.361 ± 0.156
0.723TyrTyr: 0.723 ± 0.182
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 94 proteins (16606 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski