Bacteroides phage Bacuni_F1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; unclassified Caudovirales

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D7KGD7|A0A7D7KGD7_9CAUD Uncharacterized protein OS=Bacteroides phage Bacuni_F1 OX=2759599 GN=Bacuni_F1_00034 PE=4 SV=1
MM1 pKa = 7.7ALFKK5 pKa = 11.18NFADD9 pKa = 3.67VTAADD14 pKa = 4.31LEE16 pKa = 4.43FTNIVSAIRR25 pKa = 11.84EE26 pKa = 4.06TLKK29 pKa = 11.09DD30 pKa = 4.16SFYY33 pKa = 10.69TWQEE37 pKa = 3.74YY38 pKa = 9.91PGIEE42 pKa = 4.0SSEE45 pKa = 3.88LTEE48 pKa = 4.14YY49 pKa = 10.68LGQYY53 pKa = 9.79GDD55 pKa = 4.46LYY57 pKa = 11.32EE58 pKa = 5.03DD59 pKa = 3.86ANEE62 pKa = 4.52DD63 pKa = 3.57LHH65 pKa = 8.1VVYY68 pKa = 9.92PVDD71 pKa = 3.55SKK73 pKa = 10.5MAKK76 pKa = 9.98SLANWLANYY85 pKa = 10.72DD86 pKa = 3.73MVYY89 pKa = 10.53VEE91 pKa = 4.79AGKK94 pKa = 10.65CVVSRR99 pKa = 11.84SNHH102 pKa = 5.13PAAAEE107 pKa = 4.45LINKK111 pKa = 8.5LIKK114 pKa = 10.42LAAA117 pKa = 4.43

Molecular weight: 13.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D7PPA8|A0A7D7PPA8_9CAUD Putative tail completion protein OS=Bacteroides phage Bacuni_F1 OX=2759599 GN=Bacuni_F1_00007 PE=4 SV=1
MM1 pKa = 7.38SVQIVICVKK10 pKa = 9.54MGRR13 pKa = 11.84VLSFIVMAAGIQPKK27 pKa = 10.09ARR29 pKa = 11.84AARR32 pKa = 11.84SCEE35 pKa = 3.87YY36 pKa = 10.73SATNAWNLNLNNGNANNNTKK56 pKa = 8.97ATNTNRR62 pKa = 11.84VRR64 pKa = 11.84PVSALLQITKK74 pKa = 9.09TRR76 pKa = 11.84DD77 pKa = 2.94SYY79 pKa = 11.7KK80 pKa = 10.25II81 pKa = 3.49

Molecular weight: 8.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

12447

66

863

244.1

27.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.794 ± 0.67

1.687 ± 0.147

6.315 ± 0.28

6.765 ± 0.338

2.932 ± 0.203

7.142 ± 0.33

1.486 ± 0.16

5.704 ± 0.257

6.363 ± 0.29

7.986 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.37 ± 0.119

4.451 ± 0.264

3.206 ± 0.177

3.784 ± 0.18

5.423 ± 0.399

5.311 ± 0.272

6.773 ± 0.361

6.596 ± 0.254

1.414 ± 0.112

4.499 ± 0.312
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski