Lactobacillus gastricus PS3

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae; Limosilactobacillus; Limosilactobacillus gastricus

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1269 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H4GHT9|H4GHT9_9LACO DNA-binding protein HU OS=Lactobacillus gastricus PS3 OX=1144300 GN=PS3_15348 PE=3 SV=1
MM1 pKa = 7.47SYY3 pKa = 10.38SEE5 pKa = 5.23KK6 pKa = 10.45MLDD9 pKa = 3.47QLDD12 pKa = 3.76RR13 pKa = 11.84GQLAEE18 pKa = 4.31AKK20 pKa = 10.37KK21 pKa = 10.42SFAWALRR28 pKa = 11.84KK29 pKa = 10.11DD30 pKa = 3.75DD31 pKa = 6.3DD32 pKa = 3.78EE33 pKa = 4.66TLFNLAEE40 pKa = 4.03EE41 pKa = 4.9LYY43 pKa = 11.26GLGFLNQAQRR53 pKa = 11.84VYY55 pKa = 11.27LKK57 pKa = 10.86LLEE60 pKa = 4.81KK61 pKa = 11.14YY62 pKa = 9.81PDD64 pKa = 3.52EE65 pKa = 5.03DD66 pKa = 3.69VLRR69 pKa = 11.84TALAEE74 pKa = 3.82IAIDD78 pKa = 3.61NDD80 pKa = 3.94HH81 pKa = 6.91NDD83 pKa = 3.29EE84 pKa = 4.39ALTYY88 pKa = 9.97LNQIAADD95 pKa = 3.49SDD97 pKa = 4.23AYY99 pKa = 11.29VPALLVAADD108 pKa = 4.79LYY110 pKa = 9.64QTEE113 pKa = 4.31EE114 pKa = 3.95QFEE117 pKa = 4.44VTEE120 pKa = 4.39SKK122 pKa = 10.85LLEE125 pKa = 4.71AYY127 pKa = 10.13QIAPDD132 pKa = 3.73EE133 pKa = 4.34PAVLFALGEE142 pKa = 4.19FYY144 pKa = 11.42NLIGKK149 pKa = 8.46PEE151 pKa = 3.87QAIQYY156 pKa = 8.67YY157 pKa = 9.55FALIQAGYY165 pKa = 10.92LEE167 pKa = 4.28FAKK170 pKa = 10.67VDD172 pKa = 3.01IAGRR176 pKa = 11.84LGTAYY181 pKa = 10.06AQSGHH186 pKa = 6.36FDD188 pKa = 3.05QALGYY193 pKa = 10.04LEE195 pKa = 5.13QVDD198 pKa = 4.18PQYY201 pKa = 11.06QSSDD205 pKa = 2.61IRR207 pKa = 11.84FQTGFTQLQLGKK219 pKa = 10.4LKK221 pKa = 10.6AAEE224 pKa = 4.1QSLQSLIDD232 pKa = 4.55DD233 pKa = 4.6DD234 pKa = 4.24SQYY237 pKa = 11.87ASAYY241 pKa = 7.0PALATVYY248 pKa = 9.53EE249 pKa = 4.12QEE251 pKa = 4.19NDD253 pKa = 3.31YY254 pKa = 10.96QQALKK259 pKa = 9.42TVQEE263 pKa = 4.35GLSVDD268 pKa = 3.66QYY270 pKa = 12.02NEE272 pKa = 3.55NLYY275 pKa = 10.78AQAADD280 pKa = 3.96YY281 pKa = 10.86ASHH284 pKa = 7.13LGDD287 pKa = 5.36DD288 pKa = 4.31PLMDD292 pKa = 3.41QYY294 pKa = 11.39LQRR297 pKa = 11.84AHH299 pKa = 7.24EE300 pKa = 4.45IDD302 pKa = 4.37PDD304 pKa = 3.66NLTITIQYY312 pKa = 11.13SNFLLSQQRR321 pKa = 11.84DD322 pKa = 3.44QDD324 pKa = 4.0NLNLLASVEE333 pKa = 4.1DD334 pKa = 4.53DD335 pKa = 3.54EE336 pKa = 6.03VVDD339 pKa = 5.68PQVEE343 pKa = 4.16WNRR346 pKa = 11.84AQSYY350 pKa = 7.82WRR352 pKa = 11.84LEE354 pKa = 3.98QFDD357 pKa = 3.63QAGQAFSAALPAFEE371 pKa = 5.86DD372 pKa = 3.76NPDD375 pKa = 3.95FLHH378 pKa = 6.77QLIDD382 pKa = 3.5YY383 pKa = 7.54YY384 pKa = 10.92QAVGEE389 pKa = 4.2RR390 pKa = 11.84DD391 pKa = 3.44LMFDD395 pKa = 2.93QLQRR399 pKa = 11.84YY400 pKa = 8.44VEE402 pKa = 4.19LVPTDD407 pKa = 3.23TEE409 pKa = 4.03MAEE412 pKa = 3.98RR413 pKa = 11.84LAEE416 pKa = 4.88FEE418 pKa = 4.26DD419 pKa = 3.83QMYY422 pKa = 10.91

Molecular weight: 48.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H4GIE2|H4GIE2_9LACO Uncharacterized protein OS=Lactobacillus gastricus PS3 OX=1144300 GN=PS3_17351 PE=4 SV=1
MM1 pKa = 7.92KK2 pKa = 10.14KK3 pKa = 9.69RR4 pKa = 11.84QYY6 pKa = 11.11SRR8 pKa = 11.84WLVLGIFLLAACMRR22 pKa = 11.84SPFTSLPSVIDD33 pKa = 3.39QIAASFHH40 pKa = 5.82QPATNLGILTTIPLICFGLCSTIVPSISRR69 pKa = 11.84RR70 pKa = 11.84WGNEE74 pKa = 3.11VTLGVALLIMVIGSFLRR91 pKa = 11.84IFNFVTLIFGTILVGIAITFLNVLLPAVITEE122 pKa = 5.09KK123 pKa = 10.37IPQKK127 pKa = 10.45IGLLTGMYY135 pKa = 9.5NVSLAIFSAIGAYY148 pKa = 10.27VITPVTQATNWQTAIIGFTLLIGVTFIVWLPNLRR182 pKa = 11.84FNHH185 pKa = 5.73RR186 pKa = 11.84HH187 pKa = 5.41LVPIQAGNQTRR198 pKa = 11.84IWTNPRR204 pKa = 11.84AWLLLMYY211 pKa = 10.67FGLQSFAFYY220 pKa = 10.24IIVAWLPSIAMAAGLSNATASLVASSFQLLSLPAAFLIPTITPRR264 pKa = 11.84FKK266 pKa = 11.02NRR268 pKa = 11.84VPLVIMAATPLLIGLILLLWPLNSLAYY295 pKa = 10.06FIFISFLLGTGTSATFGIVITLFGLKK321 pKa = 7.84THH323 pKa = 6.27SPEE326 pKa = 3.8EE327 pKa = 4.04TSRR330 pKa = 11.84LSGMVQSVGYY340 pKa = 9.65ILAALGPVIAGNIKK354 pKa = 10.21AATGNWQASLWMTIIIVVIMIAFGALSEE382 pKa = 4.02RR383 pKa = 11.84HH384 pKa = 5.3QYY386 pKa = 10.28INN388 pKa = 3.43

Molecular weight: 42.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1269

0

1269

425951

68

1444

335.7

37.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.348 ± 0.061

0.516 ± 0.014

6.048 ± 0.066

5.548 ± 0.073

4.001 ± 0.052

6.915 ± 0.05

2.316 ± 0.027

6.96 ± 0.064

5.28 ± 0.058

9.747 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.799 ± 0.03

4.458 ± 0.041

3.791 ± 0.033

5.488 ± 0.067

4.258 ± 0.049

5.542 ± 0.058

5.97 ± 0.047

7.329 ± 0.052

1.102 ± 0.028

3.584 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski