Amino acid dipepetide frequency for Lactobacillus gastricus PS3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.311AlaAla: 7.311 ± 0.185
0.399AlaCys: 0.399 ± 0.035
5.233AlaAsp: 5.233 ± 0.118
4.679AlaGlu: 4.679 ± 0.123
3.219AlaPhe: 3.219 ± 0.103
6.353AlaGly: 6.353 ± 0.148
1.721AlaHis: 1.721 ± 0.057
6.109AlaIle: 6.109 ± 0.133
5.242AlaLys: 5.242 ± 0.11
8.022AlaLeu: 8.022 ± 0.159
2.376AlaMet: 2.376 ± 0.059
3.897AlaAsn: 3.897 ± 0.088
2.428AlaPro: 2.428 ± 0.076
4.447AlaGln: 4.447 ± 0.122
3.09AlaArg: 3.09 ± 0.084
4.393AlaSer: 4.393 ± 0.108
5.13AlaThr: 5.13 ± 0.126
5.94AlaVal: 5.94 ± 0.129
0.798AlaTrp: 0.798 ± 0.046
2.695AlaTyr: 2.695 ± 0.078
0.0AlaXaa: 0.0 ± 0.0
Cys
0.39CysAla: 0.39 ± 0.034
0.045CysCys: 0.045 ± 0.01
0.31CysAsp: 0.31 ± 0.028
0.207CysGlu: 0.207 ± 0.022
0.284CysPhe: 0.284 ± 0.028
0.455CysGly: 0.455 ± 0.033
0.195CysHis: 0.195 ± 0.024
0.277CysIle: 0.277 ± 0.024
0.134CysLys: 0.134 ± 0.02
0.624CysLeu: 0.624 ± 0.035
0.124CysMet: 0.124 ± 0.02
0.174CysAsn: 0.174 ± 0.021
0.214CysPro: 0.214 ± 0.027
0.317CysGln: 0.317 ± 0.035
0.247CysArg: 0.247 ± 0.023
0.284CysSer: 0.284 ± 0.028
0.221CysThr: 0.221 ± 0.024
0.362CysVal: 0.362 ± 0.035
0.082CysTrp: 0.082 ± 0.016
0.211CysTyr: 0.211 ± 0.023
0.0CysXaa: 0.0 ± 0.0
Asp
4.003AspAla: 4.003 ± 0.114
0.261AspCys: 0.261 ± 0.028
3.923AspAsp: 3.923 ± 0.112
4.393AspGlu: 4.393 ± 0.129
2.803AspPhe: 2.803 ± 0.096
3.862AspGly: 3.862 ± 0.093
2.045AspHis: 2.045 ± 0.083
3.08AspIle: 3.08 ± 0.087
2.543AspLys: 2.543 ± 0.075
6.442AspLeu: 6.442 ± 0.134
1.411AspMet: 1.411 ± 0.057
2.381AspAsn: 2.381 ± 0.069
2.723AspPro: 2.723 ± 0.077
5.219AspGln: 5.219 ± 0.152
2.972AspArg: 2.972 ± 0.089
2.528AspSer: 2.528 ± 0.078
2.573AspThr: 2.573 ± 0.094
3.968AspVal: 3.968 ± 0.101
0.732AspTrp: 0.732 ± 0.044
2.62AspTyr: 2.62 ± 0.083
0.0AspXaa: 0.0 ± 0.0
Glu
4.698GluAla: 4.698 ± 0.119
0.272GluCys: 0.272 ± 0.026
2.921GluAsp: 2.921 ± 0.097
3.409GluGlu: 3.409 ± 0.104
2.172GluPhe: 2.172 ± 0.072
2.693GluGly: 2.693 ± 0.085
1.31GluHis: 1.31 ± 0.061
4.148GluIle: 4.148 ± 0.114
3.061GluLys: 3.061 ± 0.091
5.984GluLeu: 5.984 ± 0.139
1.843GluMet: 1.843 ± 0.073
2.524GluAsn: 2.524 ± 0.08
1.81GluPro: 1.81 ± 0.069
3.083GluGln: 3.083 ± 0.096
2.928GluArg: 2.928 ± 0.099
2.714GluSer: 2.714 ± 0.085
3.05GluThr: 3.05 ± 0.09
4.294GluVal: 4.294 ± 0.116
0.608GluTrp: 0.608 ± 0.044
1.958GluTyr: 1.958 ± 0.073
0.0GluXaa: 0.0 ± 0.0
Phe
3.266PheAla: 3.266 ± 0.093
0.251PheCys: 0.251 ± 0.024
2.815PheAsp: 2.815 ± 0.083
1.963PheGlu: 1.963 ± 0.075
1.775PhePhe: 1.775 ± 0.074
3.35PheGly: 3.35 ± 0.117
0.836PheHis: 0.836 ± 0.043
2.843PheIle: 2.843 ± 0.095
2.287PheLys: 2.287 ± 0.083
3.395PheLeu: 3.395 ± 0.11
1.122PheMet: 1.122 ± 0.054
2.092PheAsn: 2.092 ± 0.063
1.451PhePro: 1.451 ± 0.056
1.444PheGln: 1.444 ± 0.065
1.399PheArg: 1.399 ± 0.066
2.385PheSer: 2.385 ± 0.085
2.348PheThr: 2.348 ± 0.08
2.93PheVal: 2.93 ± 0.088
0.481PheTrp: 0.481 ± 0.038
1.575PheTyr: 1.575 ± 0.062
0.0PheXaa: 0.0 ± 0.0
Gly
5.271GlyAla: 5.271 ± 0.135
0.43GlyCys: 0.43 ± 0.037
3.881GlyAsp: 3.881 ± 0.101
3.622GlyGlu: 3.622 ± 0.094
3.043GlyPhe: 3.043 ± 0.093
4.508GlyGly: 4.508 ± 0.128
1.784GlyHis: 1.784 ± 0.067
5.538GlyIle: 5.538 ± 0.142
3.921GlyLys: 3.921 ± 0.112
6.841GlyLeu: 6.841 ± 0.153
2.071GlyMet: 2.071 ± 0.069
2.867GlyAsn: 2.867 ± 0.09
1.836GlyPro: 1.836 ± 0.062
3.754GlyGln: 3.754 ± 0.108
2.867GlyArg: 2.867 ± 0.088
3.935GlySer: 3.935 ± 0.103
4.266GlyThr: 4.266 ± 0.11
5.073GlyVal: 5.073 ± 0.12
0.838GlyTrp: 0.838 ± 0.051
2.81GlyTyr: 2.81 ± 0.083
0.0GlyXaa: 0.0 ± 0.0
His
1.601HisAla: 1.601 ± 0.056
0.122HisCys: 0.122 ± 0.018
1.446HisAsp: 1.446 ± 0.067
1.362HisGlu: 1.362 ± 0.059
1.007HisPhe: 1.007 ± 0.051
1.74HisGly: 1.74 ± 0.069
0.897HisHis: 0.897 ± 0.049
1.129HisIle: 1.129 ± 0.059
0.826HisLys: 0.826 ± 0.044
2.505HisLeu: 2.505 ± 0.081
0.423HisMet: 0.423 ± 0.029
0.93HisAsn: 0.93 ± 0.044
1.333HisPro: 1.333 ± 0.066
1.97HisGln: 1.97 ± 0.073
1.085HisArg: 1.085 ± 0.05
1.094HisSer: 1.094 ± 0.051
0.913HisThr: 0.913 ± 0.053
1.39HisVal: 1.39 ± 0.055
0.293HisTrp: 0.293 ± 0.026
1.094HisTyr: 1.094 ± 0.053
0.0HisXaa: 0.0 ± 0.0
Ile
6.019IleAla: 6.019 ± 0.119
0.542IleCys: 0.542 ± 0.039
4.625IleAsp: 4.625 ± 0.118
3.759IleGlu: 3.759 ± 0.1
2.702IlePhe: 2.702 ± 0.096
5.184IleGly: 5.184 ± 0.128
1.282IleHis: 1.282 ± 0.056
5.111IleIle: 5.111 ± 0.141
3.996IleLys: 3.996 ± 0.11
6.095IleLeu: 6.095 ± 0.149
1.817IleMet: 1.817 ± 0.068
3.662IleAsn: 3.662 ± 0.108
2.85IlePro: 2.85 ± 0.083
3.012IleGln: 3.012 ± 0.072
2.636IleArg: 2.636 ± 0.088
4.268IleSer: 4.268 ± 0.109
4.162IleThr: 4.162 ± 0.096
4.97IleVal: 4.97 ± 0.132
0.697IleTrp: 0.697 ± 0.041
2.212IleTyr: 2.212 ± 0.071
0.0IleXaa: 0.0 ± 0.0
Lys
4.458LysAla: 4.458 ± 0.104
0.157LysCys: 0.157 ± 0.022
3.05LysAsp: 3.05 ± 0.079
3.245LysGlu: 3.245 ± 0.096
1.704LysPhe: 1.704 ± 0.057
2.874LysGly: 2.874 ± 0.087
1.085LysHis: 1.085 ± 0.051
3.437LysIle: 3.437 ± 0.096
3.083LysLys: 3.083 ± 0.093
5.177LysLeu: 5.177 ± 0.105
1.965LysMet: 1.965 ± 0.07
2.453LysAsn: 2.453 ± 0.087
1.956LysPro: 1.956 ± 0.065
2.956LysGln: 2.956 ± 0.085
2.712LysArg: 2.712 ± 0.081
2.707LysSer: 2.707 ± 0.084
3.294LysThr: 3.294 ± 0.088
4.012LysVal: 4.012 ± 0.106
0.474LysTrp: 0.474 ± 0.036
2.003LysTyr: 2.003 ± 0.076
0.0LysXaa: 0.0 ± 0.0
Leu
9.414LeuAla: 9.414 ± 0.165
0.538LeuCys: 0.538 ± 0.038
5.731LeuAsp: 5.731 ± 0.128
4.672LeuGlu: 4.672 ± 0.112
3.538LeuPhe: 3.538 ± 0.097
6.834LeuGly: 6.834 ± 0.12
1.939LeuHis: 1.939 ± 0.069
6.907LeuIle: 6.907 ± 0.167
5.792LeuLys: 5.792 ± 0.103
8.642LeuLeu: 8.642 ± 0.187
2.876LeuMet: 2.876 ± 0.091
4.355LeuAsn: 4.355 ± 0.091
4.329LeuPro: 4.329 ± 0.101
4.137LeuGln: 4.137 ± 0.125
3.855LeuArg: 3.855 ± 0.104
6.365LeuSer: 6.365 ± 0.126
6.679LeuThr: 6.679 ± 0.134
7.203LeuVal: 7.203 ± 0.128
0.869LeuTrp: 0.869 ± 0.05
2.794LeuTyr: 2.794 ± 0.079
0.0LeuXaa: 0.0 ± 0.0
Met
2.653MetAla: 2.653 ± 0.076
0.136MetCys: 0.136 ± 0.02
1.613MetAsp: 1.613 ± 0.061
1.413MetGlu: 1.413 ± 0.062
0.932MetPhe: 0.932 ± 0.044
1.972MetGly: 1.972 ± 0.061
0.444MetHis: 0.444 ± 0.03
2.195MetIle: 2.195 ± 0.088
1.618MetLys: 1.618 ± 0.063
2.338MetLeu: 2.338 ± 0.086
0.96MetMet: 0.96 ± 0.047
1.359MetAsn: 1.359 ± 0.053
1.089MetPro: 1.089 ± 0.044
1.352MetGln: 1.352 ± 0.061
1.078MetArg: 1.078 ± 0.045
1.667MetSer: 1.667 ± 0.058
2.125MetThr: 2.125 ± 0.062
2.148MetVal: 2.148 ± 0.069
0.204MetTrp: 0.204 ± 0.023
0.69MetTyr: 0.69 ± 0.046
0.0MetXaa: 0.0 ± 0.0
Asn
3.061AsnAla: 3.061 ± 0.084
0.211AsnCys: 0.211 ± 0.024
2.923AsnAsp: 2.923 ± 0.085
2.611AsnGlu: 2.611 ± 0.075
1.841AsnPhe: 1.841 ± 0.057
3.566AsnGly: 3.566 ± 0.101
1.284AsnHis: 1.284 ± 0.056
2.282AsnIle: 2.282 ± 0.079
1.836AsnLys: 1.836 ± 0.063
4.371AsnLeu: 4.371 ± 0.112
1.132AsnMet: 1.132 ± 0.049
1.92AsnAsn: 1.92 ± 0.091
2.228AsnPro: 2.228 ± 0.077
3.277AsnGln: 3.277 ± 0.1
2.111AsnArg: 2.111 ± 0.084
2.244AsnSer: 2.244 ± 0.087
2.165AsnThr: 2.165 ± 0.082
2.921AsnVal: 2.921 ± 0.085
0.732AsnTrp: 0.732 ± 0.053
1.857AsnTyr: 1.857 ± 0.073
0.0AsnXaa: 0.0 ± 0.0
Pro
3.282ProAla: 3.282 ± 0.096
0.162ProCys: 0.162 ± 0.019
2.552ProAsp: 2.552 ± 0.075
2.876ProGlu: 2.876 ± 0.096
1.69ProPhe: 1.69 ± 0.074
2.524ProGly: 2.524 ± 0.085
0.716ProHis: 0.716 ± 0.045
2.686ProIle: 2.686 ± 0.08
1.897ProLys: 1.897 ± 0.071
3.439ProLeu: 3.439 ± 0.091
0.958ProMet: 0.958 ± 0.042
1.688ProAsn: 1.688 ± 0.069
0.603ProPro: 0.603 ± 0.038
2.019ProGln: 2.019 ± 0.075
1.27ProArg: 1.27 ± 0.056
2.179ProSer: 2.179 ± 0.074
2.545ProThr: 2.545 ± 0.087
3.16ProVal: 3.16 ± 0.085
0.369ProTrp: 0.369 ± 0.029
1.296ProTyr: 1.296 ± 0.056
0.0ProXaa: 0.0 ± 0.0
Gln
5.857GlnAla: 5.857 ± 0.157
0.16GlnCys: 0.16 ± 0.02
2.453GlnAsp: 2.453 ± 0.082
2.834GlnGlu: 2.834 ± 0.085
1.859GlnPhe: 1.859 ± 0.065
3.007GlnGly: 3.007 ± 0.091
1.202GlnHis: 1.202 ± 0.064
3.993GlnIle: 3.993 ± 0.095
2.721GlnLys: 2.721 ± 0.091
6.193GlnLeu: 6.193 ± 0.144
1.573GlnMet: 1.573 ± 0.058
2.035GlnAsn: 2.035 ± 0.07
2.045GlnPro: 2.045 ± 0.078
3.059GlnGln: 3.059 ± 0.105
2.648GlnArg: 2.648 ± 0.091
2.73GlnSer: 2.73 ± 0.084
3.275GlnThr: 3.275 ± 0.096
4.526GlnVal: 4.526 ± 0.119
0.646GlnTrp: 0.646 ± 0.045
2.059GlnTyr: 2.059 ± 0.077
0.0GlnXaa: 0.0 ± 0.0
Arg
2.956ArgAla: 2.956 ± 0.077
0.193ArgCys: 0.193 ± 0.025
2.413ArgAsp: 2.413 ± 0.079
2.526ArgGlu: 2.526 ± 0.095
1.838ArgPhe: 1.838 ± 0.057
2.528ArgGly: 2.528 ± 0.088
1.134ArgHis: 1.134 ± 0.063
2.913ArgIle: 2.913 ± 0.081
2.052ArgLys: 2.052 ± 0.075
4.515ArgLeu: 4.515 ± 0.11
1.16ArgMet: 1.16 ± 0.055
1.611ArgAsn: 1.611 ± 0.064
1.7ArgPro: 1.7 ± 0.069
3.144ArgGln: 3.144 ± 0.092
2.366ArgArg: 2.366 ± 0.09
2.106ArgSer: 2.106 ± 0.066
2.125ArgThr: 2.125 ± 0.071
3.094ArgVal: 3.094 ± 0.09
0.444ArgTrp: 0.444 ± 0.033
1.768ArgTyr: 1.768 ± 0.068
0.0ArgXaa: 0.0 ± 0.0
Ser
4.259SerAla: 4.259 ± 0.11
0.216SerCys: 0.216 ± 0.023
3.087SerAsp: 3.087 ± 0.087
2.747SerGlu: 2.747 ± 0.089
2.406SerPhe: 2.406 ± 0.079
4.489SerGly: 4.489 ± 0.114
1.329SerHis: 1.329 ± 0.051
3.707SerIle: 3.707 ± 0.094
2.514SerLys: 2.514 ± 0.08
5.712SerLeu: 5.712 ± 0.115
1.491SerMet: 1.491 ± 0.055
2.411SerAsn: 2.411 ± 0.091
2.129SerPro: 2.129 ± 0.073
3.031SerGln: 3.031 ± 0.098
2.399SerArg: 2.399 ± 0.078
3.693SerSer: 3.693 ± 0.17
3.355SerThr: 3.355 ± 0.112
3.738SerVal: 3.738 ± 0.097
0.718SerTrp: 0.718 ± 0.04
1.988SerTyr: 1.988 ± 0.066
0.0SerXaa: 0.0 ± 0.0
Thr
4.871ThrAla: 4.871 ± 0.123
0.317ThrCys: 0.317 ± 0.032
3.7ThrAsp: 3.7 ± 0.085
2.921ThrGlu: 2.921 ± 0.086
2.247ThrPhe: 2.247 ± 0.079
4.571ThrGly: 4.571 ± 0.118
1.153ThrHis: 1.153 ± 0.053
4.85ThrIle: 4.85 ± 0.113
3.486ThrLys: 3.486 ± 0.099
5.339ThrLeu: 5.339 ± 0.124
1.528ThrMet: 1.528 ± 0.057
2.977ThrAsn: 2.977 ± 0.087
2.557ThrPro: 2.557 ± 0.095
2.08ThrGln: 2.08 ± 0.076
1.991ThrArg: 1.991 ± 0.07
3.634ThrSer: 3.634 ± 0.104
4.099ThrThr: 4.099 ± 0.11
4.653ThrVal: 4.653 ± 0.121
0.667ThrTrp: 0.667 ± 0.04
2.061ThrTyr: 2.061 ± 0.071
0.0ThrXaa: 0.0 ± 0.0
Val
6.923ValAla: 6.923 ± 0.142
0.451ValCys: 0.451 ± 0.036
4.855ValAsp: 4.855 ± 0.125
4.008ValGlu: 4.008 ± 0.112
2.606ValPhe: 2.606 ± 0.082
5.116ValGly: 5.116 ± 0.117
1.357ValHis: 1.357 ± 0.059
5.803ValIle: 5.803 ± 0.135
4.209ValLys: 4.209 ± 0.105
6.289ValLeu: 6.289 ± 0.112
2.127ValMet: 2.127 ± 0.069
3.428ValAsn: 3.428 ± 0.102
2.744ValPro: 2.744 ± 0.076
2.996ValGln: 2.996 ± 0.083
2.519ValArg: 2.519 ± 0.082
4.076ValSer: 4.076 ± 0.118
4.968ValThr: 4.968 ± 0.105
5.879ValVal: 5.879 ± 0.149
0.688ValTrp: 0.688 ± 0.042
2.254ValTyr: 2.254 ± 0.074
0.0ValXaa: 0.0 ± 0.0
Trp
0.676TrpAla: 0.676 ± 0.04
0.066TrpCys: 0.066 ± 0.014
0.629TrpAsp: 0.629 ± 0.044
0.462TrpGlu: 0.462 ± 0.036
0.599TrpPhe: 0.599 ± 0.04
0.779TrpGly: 0.779 ± 0.052
0.319TrpHis: 0.319 ± 0.026
0.681TrpIle: 0.681 ± 0.041
0.387TrpLys: 0.387 ± 0.03
1.51TrpLeu: 1.51 ± 0.083
0.343TrpMet: 0.343 ± 0.031
0.441TrpAsn: 0.441 ± 0.031
0.362TrpPro: 0.362 ± 0.034
0.695TrpGln: 0.695 ± 0.044
0.545TrpArg: 0.545 ± 0.039
0.582TrpSer: 0.582 ± 0.037
0.589TrpThr: 0.589 ± 0.036
0.751TrpVal: 0.751 ± 0.044
0.209TrpTrp: 0.209 ± 0.024
0.397TrpTyr: 0.397 ± 0.037
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.51TyrAla: 2.51 ± 0.09
0.228TyrCys: 0.228 ± 0.025
2.322TyrAsp: 2.322 ± 0.078
1.773TyrGlu: 1.773 ± 0.064
1.742TyrPhe: 1.742 ± 0.071
2.749TyrGly: 2.749 ± 0.087
1.129TyrHis: 1.129 ± 0.056
1.911TyrIle: 1.911 ± 0.071
1.195TyrLys: 1.195 ± 0.061
4.033TyrLeu: 4.033 ± 0.109
0.686TyrMet: 0.686 ± 0.038
1.371TyrAsn: 1.371 ± 0.06
1.524TyrPro: 1.524 ± 0.067
2.888TyrGln: 2.888 ± 0.097
1.869TyrArg: 1.869 ± 0.067
1.836TyrSer: 1.836 ± 0.08
1.822TyrThr: 1.822 ± 0.079
2.284TyrVal: 2.284 ± 0.073
0.472TyrTrp: 0.472 ± 0.036
1.503TyrTyr: 1.503 ± 0.074
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1269 proteins (425951 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski