Ostreococcus lucimarinus (strain CCE9901)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Chlorophyta; Mamiellophyceae; Mamiellales; Bathycoccaceae; Ostreococcus; unclassified Ostreococcus; Ostreococcus sp. 'lucimarinus'

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7403 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A4S1I3|A4S1I3_OSTLU Carbamoyl-phosphate synthase (glutamine-hydrolyzing) OS=Ostreococcus lucimarinus (strain CCE9901) OX=436017 GN=PYR1L PE=3 SV=1
MM1 pKa = 7.29TIEE4 pKa = 4.12YY5 pKa = 8.46TPSEE9 pKa = 4.32SEE11 pKa = 3.9EE12 pKa = 4.71GEE14 pKa = 3.89GGMFARR20 pKa = 11.84LPSQGAGANAGLYY33 pKa = 9.82EE34 pKa = 4.26YY35 pKa = 10.51EE36 pKa = 5.26DD37 pKa = 5.27DD38 pKa = 5.38SDD40 pKa = 5.45SSDD43 pKa = 3.66DD44 pKa = 4.02DD45 pKa = 3.65VRR47 pKa = 11.84HH48 pKa = 5.73PRR50 pKa = 11.84ARR52 pKa = 11.84DD53 pKa = 3.48DD54 pKa = 5.6APSCCCVITT63 pKa = 4.11

Molecular weight: 6.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A4RXY3|A4RXY3_OSTLU Homeobox domain-containing protein OS=Ostreococcus lucimarinus (strain CCE9901) OX=436017 GN=OSTLU_15538 PE=4 SV=1
MM1 pKa = 7.67RR2 pKa = 11.84AQRR5 pKa = 11.84GALVVTAGGKK15 pKa = 10.25SIGCTLGGTRR25 pKa = 11.84RR26 pKa = 11.84KK27 pKa = 9.69RR28 pKa = 11.84ARR30 pKa = 11.84TSGFRR35 pKa = 11.84TRR37 pKa = 11.84IASASGRR44 pKa = 11.84KK45 pKa = 8.19VLKK48 pKa = 10.09NRR50 pKa = 11.84RR51 pKa = 11.84AKK53 pKa = 9.61GRR55 pKa = 11.84KK56 pKa = 8.24VLAPAGAVRR65 pKa = 11.84GWNKK69 pKa = 9.47EE70 pKa = 3.71KK71 pKa = 10.79KK72 pKa = 9.96

Molecular weight: 7.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7403

0

7403

2987645

35

18193

403.6

44.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.905 ± 0.051

1.675 ± 0.017

6.384 ± 0.027

7.041 ± 0.039

3.55 ± 0.019

7.163 ± 0.037

1.958 ± 0.018

3.909 ± 0.022

4.914 ± 0.029

8.416 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.401 ± 0.014

2.992 ± 0.018

4.227 ± 0.026

2.652 ± 0.017

7.462 ± 0.034

6.731 ± 0.044

5.698 ± 0.024

7.347 ± 0.022

1.22 ± 0.01

2.354 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski