Methylocystis parvus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylocystaceae; Methylocystis

Average proteome isoelectric point is 6.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4128 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6B8MDV0|A0A6B8MDV0_9RHIZ GNAT family N-acetyltransferase OS=Methylocystis parvus OX=134 GN=F7D14_01170 PE=4 SV=1
MM1 pKa = 6.85MAEE4 pKa = 4.72FKK6 pKa = 10.46TRR8 pKa = 11.84DD9 pKa = 3.46GSPYY13 pKa = 9.26TPLYY17 pKa = 9.38LTDD20 pKa = 4.82INAEE24 pKa = 4.1TCIGCGRR31 pKa = 11.84CFKK34 pKa = 10.63VCPQGVMALYY44 pKa = 10.13GVNDD48 pKa = 3.63EE49 pKa = 4.89GEE51 pKa = 3.9ILGIVTEE58 pKa = 5.13DD59 pKa = 4.93DD60 pKa = 5.16DD61 pKa = 7.5DD62 pKa = 6.7DD63 pKa = 5.9FDD65 pKa = 6.93GDD67 pKa = 4.15LNRR70 pKa = 11.84KK71 pKa = 7.73IMKK74 pKa = 9.72VEE76 pKa = 3.93SAGACIGCNACARR89 pKa = 11.84VCPKK93 pKa = 10.29NCQTHH98 pKa = 5.58VPADD102 pKa = 4.27EE103 pKa = 4.21IAAA106 pKa = 4.07

Molecular weight: 11.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6B8MFV9|A0A6B8MFV9_9RHIZ MFS transporter OS=Methylocystis parvus OX=134 GN=F7D14_21000 PE=4 SV=1
MM1 pKa = 6.89WRR3 pKa = 11.84KK4 pKa = 8.82PGRR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84TPRR12 pKa = 11.84SKK14 pKa = 10.49KK15 pKa = 9.67PRR17 pKa = 11.84PTRR20 pKa = 11.84PTRR23 pKa = 11.84PPPPRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 9.58PRR32 pKa = 11.84TSTSWSRR39 pKa = 11.84TQRR42 pKa = 11.84RR43 pKa = 11.84KK44 pKa = 9.9SPSRR48 pKa = 11.84RR49 pKa = 11.84TRR51 pKa = 11.84RR52 pKa = 11.84PTTRR56 pKa = 11.84SRR58 pKa = 11.84KK59 pKa = 8.9RR60 pKa = 11.84ARR62 pKa = 11.84TSCSAMM68 pKa = 3.42

Molecular weight: 8.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4128

0

4128

1274671

25

2845

308.8

33.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.392 ± 0.062

0.917 ± 0.013

5.609 ± 0.031

5.95 ± 0.044

3.999 ± 0.028

8.233 ± 0.039

1.941 ± 0.019

4.876 ± 0.027

3.636 ± 0.032

10.023 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.206 ± 0.019

2.612 ± 0.026

5.307 ± 0.034

2.892 ± 0.023

7.499 ± 0.044

5.522 ± 0.027

4.732 ± 0.028

7.044 ± 0.034

1.324 ± 0.016

2.288 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski