Gordonia phage TZGordon

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vendettavirus; unclassified Vendettavirus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N0A596|A0A6N0A596_9CAUD Uncharacterized protein OS=Gordonia phage TZGordon OX=2744004 GN=57 PE=4 SV=1
MM1 pKa = 7.68NDD3 pKa = 2.8HH4 pKa = 6.48TVPVPARR11 pKa = 11.84VLVNVIDD18 pKa = 4.23ALSTLVLDD26 pKa = 4.7VYY28 pKa = 11.26ADD30 pKa = 3.63EE31 pKa = 5.84PIPFVPTWTDD41 pKa = 3.28EE42 pKa = 4.25LPDD45 pKa = 4.09GFEE48 pKa = 4.8LPDD51 pKa = 4.27GLDD54 pKa = 3.56EE55 pKa = 4.64PATPAEE61 pKa = 4.36FHH63 pKa = 6.5SAPAGEE69 pKa = 5.52DD70 pKa = 3.0VDD72 pKa = 6.02ADD74 pKa = 4.16DD75 pKa = 6.31LLDD78 pKa = 3.7TLRR81 pKa = 11.84SRR83 pKa = 11.84LGLDD87 pKa = 3.29PEE89 pKa = 4.19PAEE92 pKa = 3.96FGVYY96 pKa = 8.97RR97 pKa = 11.84DD98 pKa = 3.29RR99 pKa = 11.84VGDD102 pKa = 3.65VWRR105 pKa = 11.84HH106 pKa = 5.08TPDD109 pKa = 2.4GWQVFEE115 pKa = 4.6TSDD118 pKa = 3.53GHH120 pKa = 7.12DD121 pKa = 3.48VTHH124 pKa = 6.41LTVEE128 pKa = 4.5TSWCPEE134 pKa = 3.89VADD137 pKa = 4.64CGPFEE142 pKa = 5.19LVDD145 pKa = 3.9SEE147 pKa = 4.39PASFSLAEE155 pKa = 4.27AAHH158 pKa = 5.97QLAEE162 pKa = 4.08QGIDD166 pKa = 2.94TGRR169 pKa = 11.84NRR171 pKa = 11.84LYY173 pKa = 10.45RR174 pKa = 11.84YY175 pKa = 9.74LNEE178 pKa = 4.87GIAWTDD184 pKa = 3.58GEE186 pKa = 4.61GSPRR190 pKa = 11.84PAADD194 pKa = 3.96GYY196 pKa = 11.27LIVAEE201 pKa = 4.28PASGSRR207 pKa = 11.84SAVVRR212 pKa = 11.84VTPEE216 pKa = 3.45GVAALARR223 pKa = 11.84VMGSALL229 pKa = 3.38

Molecular weight: 24.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N0A7E2|A0A6N0A7E2_9CAUD Terminase large subunit OS=Gordonia phage TZGordon OX=2744004 GN=9 PE=4 SV=1
MM1 pKa = 6.42TAKK4 pKa = 10.53GAGRR8 pKa = 11.84STRR11 pKa = 11.84RR12 pKa = 11.84WRR14 pKa = 11.84RR15 pKa = 11.84LKK17 pKa = 10.92AEE19 pKa = 4.16FRR21 pKa = 11.84RR22 pKa = 11.84LCADD26 pKa = 3.38RR27 pKa = 11.84RR28 pKa = 11.84APCWICGQPIDD39 pKa = 3.96YY40 pKa = 10.18RR41 pKa = 11.84LEE43 pKa = 4.39FPHH46 pKa = 6.92PEE48 pKa = 3.49AWEE51 pKa = 4.18PDD53 pKa = 3.45HH54 pKa = 5.81YY55 pKa = 11.25HH56 pKa = 6.58PVAKK60 pKa = 10.12RR61 pKa = 11.84PEE63 pKa = 4.16LAEE66 pKa = 4.64DD67 pKa = 3.77PANLRR72 pKa = 11.84PSHH75 pKa = 6.16SSCNRR80 pKa = 11.84SRR82 pKa = 11.84GDD84 pKa = 3.41RR85 pKa = 11.84EE86 pKa = 4.17VVLGLGRR93 pKa = 11.84QSRR96 pKa = 11.84QWW98 pKa = 3.01

Molecular weight: 11.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

14106

37

1152

172.0

18.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.633 ± 0.526

0.78 ± 0.116

6.912 ± 0.261

6.593 ± 0.328

2.935 ± 0.163

9.613 ± 0.517

1.95 ± 0.174

3.374 ± 0.174

3.601 ± 0.227

7.444 ± 0.257

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.332 ± 0.132

2.736 ± 0.194

4.714 ± 0.224

3.063 ± 0.174

7.819 ± 0.387

5.14 ± 0.231

5.721 ± 0.205

8.145 ± 0.292

1.971 ± 0.11

2.524 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski