Amino acid dipepetide frequency for Gordonia phage TZGordon

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
17.37AlaAla: 17.37 ± 1.702
0.638AlaCys: 0.638 ± 0.257
8.224AlaAsp: 8.224 ± 0.999
9.784AlaGlu: 9.784 ± 1.038
3.758AlaPhe: 3.758 ± 0.535
14.534AlaGly: 14.534 ± 1.574
2.481AlaHis: 2.481 ± 0.442
4.183AlaIle: 4.183 ± 0.649
5.955AlaLys: 5.955 ± 0.879
9.784AlaLeu: 9.784 ± 0.839
2.907AlaMet: 2.907 ± 0.52
3.616AlaAsn: 3.616 ± 0.498
4.183AlaPro: 4.183 ± 0.589
3.545AlaGln: 3.545 ± 0.454
9.287AlaArg: 9.287 ± 0.877
5.814AlaSer: 5.814 ± 0.745
6.452AlaThr: 6.452 ± 0.705
8.508AlaVal: 8.508 ± 1.056
2.056AlaTrp: 2.056 ± 0.347
3.19AlaTyr: 3.19 ± 0.471
0.0AlaXaa: 0.0 ± 0.0
Cys
0.709CysAla: 0.709 ± 0.227
0.071CysCys: 0.071 ± 0.078
0.567CysAsp: 0.567 ± 0.216
0.496CysGlu: 0.496 ± 0.175
0.071CysPhe: 0.071 ± 0.064
1.276CysGly: 1.276 ± 0.354
0.284CysHis: 0.284 ± 0.127
0.284CysIle: 0.284 ± 0.117
0.425CysLys: 0.425 ± 0.198
0.354CysLeu: 0.354 ± 0.159
0.071CysMet: 0.071 ± 0.068
0.354CysAsn: 0.354 ± 0.128
0.354CysPro: 0.354 ± 0.196
0.213CysGln: 0.213 ± 0.107
0.496CysArg: 0.496 ± 0.192
0.638CysSer: 0.638 ± 0.196
0.142CysThr: 0.142 ± 0.112
0.425CysVal: 0.425 ± 0.197
0.354CysTrp: 0.354 ± 0.143
0.213CysTyr: 0.213 ± 0.122
0.0CysXaa: 0.0 ± 0.0
Asp
9.146AspAla: 9.146 ± 0.891
0.993AspCys: 0.993 ± 0.209
4.467AspAsp: 4.467 ± 0.575
6.593AspGlu: 6.593 ± 0.906
1.843AspPhe: 1.843 ± 0.394
6.31AspGly: 6.31 ± 0.645
1.418AspHis: 1.418 ± 0.36
1.134AspIle: 1.134 ± 0.298
1.985AspLys: 1.985 ± 0.372
5.034AspLeu: 5.034 ± 0.671
1.418AspMet: 1.418 ± 0.395
0.922AspAsn: 0.922 ± 0.195
4.467AspPro: 4.467 ± 0.476
3.119AspGln: 3.119 ± 0.45
5.743AspArg: 5.743 ± 0.629
3.474AspSer: 3.474 ± 0.495
3.049AspThr: 3.049 ± 0.47
5.814AspVal: 5.814 ± 0.637
0.78AspTrp: 0.78 ± 0.22
1.418AspTyr: 1.418 ± 0.318
0.0AspXaa: 0.0 ± 0.0
Glu
8.224GluAla: 8.224 ± 0.729
0.567GluCys: 0.567 ± 0.227
3.899GluAsp: 3.899 ± 0.573
2.34GluGlu: 2.34 ± 0.468
2.552GluPhe: 2.552 ± 0.392
5.317GluGly: 5.317 ± 0.728
1.985GluHis: 1.985 ± 0.361
1.489GluIle: 1.489 ± 0.377
1.631GluLys: 1.631 ± 0.325
7.019GluLeu: 7.019 ± 0.843
1.418GluMet: 1.418 ± 0.339
1.418GluAsn: 1.418 ± 0.302
2.978GluPro: 2.978 ± 0.48
2.41GluGln: 2.41 ± 0.418
5.955GluArg: 5.955 ± 0.669
4.112GluSer: 4.112 ± 0.606
3.403GluThr: 3.403 ± 0.548
5.672GluVal: 5.672 ± 0.687
1.772GluTrp: 1.772 ± 0.378
1.772GluTyr: 1.772 ± 0.37
0.0GluXaa: 0.0 ± 0.0
Phe
4.041PheAla: 4.041 ± 0.498
0.213PheCys: 0.213 ± 0.122
1.489PheAsp: 1.489 ± 0.278
2.907PheGlu: 2.907 ± 0.474
0.567PhePhe: 0.567 ± 0.173
2.41PheGly: 2.41 ± 0.506
0.638PheHis: 0.638 ± 0.197
0.851PheIle: 0.851 ± 0.196
0.922PheLys: 0.922 ± 0.264
1.702PheLeu: 1.702 ± 0.366
1.063PheMet: 1.063 ± 0.268
0.496PheAsn: 0.496 ± 0.185
0.851PhePro: 0.851 ± 0.246
0.993PheGln: 0.993 ± 0.278
2.765PheArg: 2.765 ± 0.365
1.631PheSer: 1.631 ± 0.296
2.765PheThr: 2.765 ± 0.445
2.127PheVal: 2.127 ± 0.453
0.425PheTrp: 0.425 ± 0.154
0.496PheTyr: 0.496 ± 0.213
0.0PheXaa: 0.0 ± 0.0
Gly
9.287GlyAla: 9.287 ± 1.161
0.638GlyCys: 0.638 ± 0.188
7.87GlyAsp: 7.87 ± 0.688
6.168GlyGlu: 6.168 ± 0.543
2.907GlyPhe: 2.907 ± 0.47
11.343GlyGly: 11.343 ± 1.639
1.702GlyHis: 1.702 ± 0.38
3.261GlyIle: 3.261 ± 0.471
4.608GlyLys: 4.608 ± 0.603
5.884GlyLeu: 5.884 ± 0.64
3.049GlyMet: 3.049 ± 0.414
2.907GlyAsn: 2.907 ± 0.591
4.396GlyPro: 4.396 ± 0.679
3.687GlyGln: 3.687 ± 0.601
6.877GlyArg: 6.877 ± 0.543
5.246GlySer: 5.246 ± 0.549
4.679GlyThr: 4.679 ± 0.481
7.231GlyVal: 7.231 ± 0.908
1.914GlyTrp: 1.914 ± 0.397
2.481GlyTyr: 2.481 ± 0.456
0.0GlyXaa: 0.0 ± 0.0
His
2.481HisAla: 2.481 ± 0.489
0.142HisCys: 0.142 ± 0.114
1.418HisAsp: 1.418 ± 0.301
0.993HisGlu: 0.993 ± 0.252
0.638HisPhe: 0.638 ± 0.18
1.914HisGly: 1.914 ± 0.401
0.354HisHis: 0.354 ± 0.158
0.425HisIle: 0.425 ± 0.173
0.709HisLys: 0.709 ± 0.234
1.134HisLeu: 1.134 ± 0.216
0.284HisMet: 0.284 ± 0.112
0.567HisAsn: 0.567 ± 0.181
0.922HisPro: 0.922 ± 0.286
0.638HisGln: 0.638 ± 0.206
1.276HisArg: 1.276 ± 0.322
0.78HisSer: 0.78 ± 0.197
0.993HisThr: 0.993 ± 0.255
2.623HisVal: 2.623 ± 0.53
0.638HisTrp: 0.638 ± 0.225
0.567HisTyr: 0.567 ± 0.17
0.0HisXaa: 0.0 ± 0.0
Ile
5.743IleAla: 5.743 ± 0.683
0.213IleCys: 0.213 ± 0.105
2.269IleAsp: 2.269 ± 0.362
3.403IleGlu: 3.403 ± 0.524
0.567IlePhe: 0.567 ± 0.175
3.474IleGly: 3.474 ± 0.534
0.567IleHis: 0.567 ± 0.174
0.922IleIle: 0.922 ± 0.228
0.284IleLys: 0.284 ± 0.12
2.056IleLeu: 2.056 ± 0.372
0.709IleMet: 0.709 ± 0.246
0.354IleAsn: 0.354 ± 0.165
2.765IlePro: 2.765 ± 0.429
1.276IleGln: 1.276 ± 0.321
1.772IleArg: 1.772 ± 0.395
1.56IleSer: 1.56 ± 0.33
2.127IleThr: 2.127 ± 0.322
2.694IleVal: 2.694 ± 0.352
0.213IleTrp: 0.213 ± 0.117
0.78IleTyr: 0.78 ± 0.269
0.0IleXaa: 0.0 ± 0.0
Lys
5.105LysAla: 5.105 ± 0.741
0.354LysCys: 0.354 ± 0.147
2.056LysAsp: 2.056 ± 0.435
0.709LysGlu: 0.709 ± 0.206
1.063LysPhe: 1.063 ± 0.426
3.616LysGly: 3.616 ± 0.376
0.567LysHis: 0.567 ± 0.228
0.922LysIle: 0.922 ± 0.265
0.709LysLys: 0.709 ± 0.356
3.19LysLeu: 3.19 ± 0.391
0.78LysMet: 0.78 ± 0.23
0.993LysAsn: 0.993 ± 0.315
2.34LysPro: 2.34 ± 0.452
0.851LysGln: 0.851 ± 0.297
3.97LysArg: 3.97 ± 0.655
2.552LysSer: 2.552 ± 0.371
1.56LysThr: 1.56 ± 0.42
3.261LysVal: 3.261 ± 0.372
0.567LysTrp: 0.567 ± 0.217
0.851LysTyr: 0.851 ± 0.251
0.0LysXaa: 0.0 ± 0.0
Leu
10.776LeuAla: 10.776 ± 0.832
0.922LeuCys: 0.922 ± 0.285
6.523LeuAsp: 6.523 ± 0.704
3.474LeuGlu: 3.474 ± 0.522
1.843LeuPhe: 1.843 ± 0.384
6.381LeuGly: 6.381 ± 0.669
1.276LeuHis: 1.276 ± 0.364
2.552LeuIle: 2.552 ± 0.33
2.623LeuLys: 2.623 ± 0.435
4.183LeuLeu: 4.183 ± 0.566
1.772LeuMet: 1.772 ± 0.369
2.056LeuAsn: 2.056 ± 0.443
3.828LeuPro: 3.828 ± 0.506
1.489LeuGln: 1.489 ± 0.287
6.026LeuArg: 6.026 ± 0.674
4.821LeuSer: 4.821 ± 0.551
5.105LeuThr: 5.105 ± 0.577
4.821LeuVal: 4.821 ± 0.539
1.56LeuTrp: 1.56 ± 0.342
2.41LeuTyr: 2.41 ± 0.432
0.0LeuXaa: 0.0 ± 0.0
Met
3.261MetAla: 3.261 ± 0.544
0.071MetCys: 0.071 ± 0.075
1.347MetAsp: 1.347 ± 0.333
0.78MetGlu: 0.78 ± 0.229
0.638MetPhe: 0.638 ± 0.163
1.56MetGly: 1.56 ± 0.285
0.425MetHis: 0.425 ± 0.186
0.851MetIle: 0.851 ± 0.262
0.922MetLys: 0.922 ± 0.267
1.631MetLeu: 1.631 ± 0.349
0.496MetMet: 0.496 ± 0.177
1.347MetAsn: 1.347 ± 0.267
0.922MetPro: 0.922 ± 0.291
0.78MetGln: 0.78 ± 0.203
1.914MetArg: 1.914 ± 0.308
1.631MetSer: 1.631 ± 0.331
1.843MetThr: 1.843 ± 0.405
1.631MetVal: 1.631 ± 0.348
0.78MetTrp: 0.78 ± 0.223
0.496MetTyr: 0.496 ± 0.165
0.0MetXaa: 0.0 ± 0.0
Asn
2.623AsnAla: 2.623 ± 0.383
0.071AsnCys: 0.071 ± 0.069
1.772AsnAsp: 1.772 ± 0.335
1.631AsnGlu: 1.631 ± 0.35
0.993AsnPhe: 0.993 ± 0.274
3.828AsnGly: 3.828 ± 0.463
0.496AsnHis: 0.496 ± 0.194
0.922AsnIle: 0.922 ± 0.291
0.851AsnLys: 0.851 ± 0.262
1.914AsnLeu: 1.914 ± 0.449
0.638AsnMet: 0.638 ± 0.198
0.425AsnAsn: 0.425 ± 0.225
2.552AsnPro: 2.552 ± 0.403
0.851AsnGln: 0.851 ± 0.27
1.914AsnArg: 1.914 ± 0.4
1.205AsnSer: 1.205 ± 0.285
1.631AsnThr: 1.631 ± 0.471
2.269AsnVal: 2.269 ± 0.375
0.142AsnTrp: 0.142 ± 0.09
0.638AsnTyr: 0.638 ± 0.204
0.0AsnXaa: 0.0 ± 0.0
Pro
7.019ProAla: 7.019 ± 0.905
0.496ProCys: 0.496 ± 0.184
3.97ProAsp: 3.97 ± 0.554
3.687ProGlu: 3.687 ± 0.511
1.489ProPhe: 1.489 ± 0.292
4.396ProGly: 4.396 ± 0.501
1.063ProHis: 1.063 ± 0.255
2.41ProIle: 2.41 ± 0.433
1.772ProLys: 1.772 ± 0.517
2.907ProLeu: 2.907 ± 0.474
0.993ProMet: 0.993 ± 0.278
1.134ProAsn: 1.134 ± 0.305
1.489ProPro: 1.489 ± 0.386
1.347ProGln: 1.347 ± 0.274
2.623ProArg: 2.623 ± 0.439
1.631ProSer: 1.631 ± 0.284
2.41ProThr: 2.41 ± 0.362
4.041ProVal: 4.041 ± 0.48
1.063ProTrp: 1.063 ± 0.284
1.205ProTyr: 1.205 ± 0.261
0.0ProXaa: 0.0 ± 0.0
Gln
3.403GlnAla: 3.403 ± 0.506
0.071GlnCys: 0.071 ± 0.074
1.418GlnAsp: 1.418 ± 0.32
0.851GlnGlu: 0.851 ± 0.221
0.851GlnPhe: 0.851 ± 0.226
2.198GlnGly: 2.198 ± 0.375
0.567GlnHis: 0.567 ± 0.188
0.993GlnIle: 0.993 ± 0.212
0.78GlnLys: 0.78 ± 0.249
3.19GlnLeu: 3.19 ± 0.491
0.638GlnMet: 0.638 ± 0.221
0.922GlnAsn: 0.922 ± 0.215
1.772GlnPro: 1.772 ± 0.35
1.418GlnGln: 1.418 ± 0.364
3.261GlnArg: 3.261 ± 0.565
2.127GlnSer: 2.127 ± 0.441
2.127GlnThr: 2.127 ± 0.418
2.41GlnVal: 2.41 ± 0.448
0.78GlnTrp: 0.78 ± 0.199
0.851GlnTyr: 0.851 ± 0.266
0.0GlnXaa: 0.0 ± 0.0
Arg
9.996ArgAla: 9.996 ± 0.965
0.638ArgCys: 0.638 ± 0.261
5.175ArgAsp: 5.175 ± 0.715
6.026ArgGlu: 6.026 ± 0.737
2.836ArgPhe: 2.836 ± 0.355
6.381ArgGly: 6.381 ± 0.852
1.489ArgHis: 1.489 ± 0.372
3.545ArgIle: 3.545 ± 0.412
3.19ArgLys: 3.19 ± 0.514
5.175ArgLeu: 5.175 ± 0.767
1.914ArgMet: 1.914 ± 0.376
2.481ArgAsn: 2.481 ± 0.445
3.19ArgPro: 3.19 ± 0.508
1.56ArgGln: 1.56 ± 0.309
7.373ArgArg: 7.373 ± 0.927
2.907ArgSer: 2.907 ± 0.417
4.254ArgThr: 4.254 ± 0.508
6.452ArgVal: 6.452 ± 0.833
1.134ArgTrp: 1.134 ± 0.301
2.481ArgTyr: 2.481 ± 0.385
0.0ArgXaa: 0.0 ± 0.0
Ser
7.444SerAla: 7.444 ± 1.093
0.567SerCys: 0.567 ± 0.196
4.467SerAsp: 4.467 ± 0.636
3.19SerGlu: 3.19 ± 0.519
1.205SerPhe: 1.205 ± 0.232
5.246SerGly: 5.246 ± 0.567
0.709SerHis: 0.709 ± 0.23
1.985SerIle: 1.985 ± 0.363
1.347SerLys: 1.347 ± 0.308
4.112SerLeu: 4.112 ± 0.579
1.205SerMet: 1.205 ± 0.248
1.631SerAsn: 1.631 ± 0.298
1.772SerPro: 1.772 ± 0.342
1.276SerGln: 1.276 ± 0.23
3.119SerArg: 3.119 ± 0.426
2.623SerSer: 2.623 ± 0.436
2.836SerThr: 2.836 ± 0.487
4.254SerVal: 4.254 ± 0.548
1.489SerTrp: 1.489 ± 0.37
0.922SerTyr: 0.922 ± 0.208
0.0SerXaa: 0.0 ± 0.0
Thr
5.884ThrAla: 5.884 ± 0.835
0.284ThrCys: 0.284 ± 0.138
3.828ThrAsp: 3.828 ± 0.507
3.403ThrGlu: 3.403 ± 0.494
2.127ThrPhe: 2.127 ± 0.423
4.821ThrGly: 4.821 ± 0.545
0.78ThrHis: 0.78 ± 0.261
2.694ThrIle: 2.694 ± 0.443
1.843ThrLys: 1.843 ± 0.392
4.537ThrLeu: 4.537 ± 0.476
0.993ThrMet: 0.993 ± 0.284
1.843ThrAsn: 1.843 ± 0.434
3.332ThrPro: 3.332 ± 0.483
1.205ThrGln: 1.205 ± 0.321
3.474ThrArg: 3.474 ± 0.524
2.056ThrSer: 2.056 ± 0.387
4.183ThrThr: 4.183 ± 1.001
6.664ThrVal: 6.664 ± 0.628
1.347ThrTrp: 1.347 ± 0.316
1.914ThrTyr: 1.914 ± 0.375
0.0ThrXaa: 0.0 ± 0.0
Val
9.571ValAla: 9.571 ± 0.747
0.496ValCys: 0.496 ± 0.223
5.317ValAsp: 5.317 ± 0.645
6.381ValGlu: 6.381 ± 0.789
1.914ValPhe: 1.914 ± 0.357
6.239ValGly: 6.239 ± 0.665
1.843ValHis: 1.843 ± 0.452
3.261ValIle: 3.261 ± 0.571
3.828ValLys: 3.828 ± 0.672
6.664ValLeu: 6.664 ± 0.84
1.985ValMet: 1.985 ± 0.305
2.34ValAsn: 2.34 ± 0.489
3.616ValPro: 3.616 ± 0.527
2.127ValGln: 2.127 ± 0.335
5.459ValArg: 5.459 ± 0.679
4.396ValSer: 4.396 ± 0.579
5.317ValThr: 5.317 ± 0.75
7.373ValVal: 7.373 ± 0.875
1.772ValTrp: 1.772 ± 0.365
1.56ValTyr: 1.56 ± 0.346
0.0ValXaa: 0.0 ± 0.0
Trp
1.702TrpAla: 1.702 ± 0.36
0.142TrpCys: 0.142 ± 0.099
1.489TrpAsp: 1.489 ± 0.296
1.418TrpGlu: 1.418 ± 0.346
0.496TrpPhe: 0.496 ± 0.199
1.489TrpGly: 1.489 ± 0.296
0.425TrpHis: 0.425 ± 0.185
0.567TrpIle: 0.567 ± 0.186
0.851TrpLys: 0.851 ± 0.265
2.127TrpLeu: 2.127 ± 0.393
0.284TrpMet: 0.284 ± 0.144
0.709TrpAsn: 0.709 ± 0.282
0.709TrpPro: 0.709 ± 0.238
0.922TrpGln: 0.922 ± 0.245
1.985TrpArg: 1.985 ± 0.414
1.134TrpSer: 1.134 ± 0.295
1.56TrpThr: 1.56 ± 0.349
0.922TrpVal: 0.922 ± 0.278
0.284TrpTrp: 0.284 ± 0.125
0.496TrpTyr: 0.496 ± 0.178
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.552TyrAla: 2.552 ± 0.385
0.213TyrCys: 0.213 ± 0.118
1.56TyrAsp: 1.56 ± 0.326
1.702TyrGlu: 1.702 ± 0.305
0.993TyrPhe: 0.993 ± 0.239
3.403TyrGly: 3.403 ± 0.489
0.425TyrHis: 0.425 ± 0.162
0.496TyrIle: 0.496 ± 0.161
0.78TyrLys: 0.78 ± 0.232
1.843TyrLeu: 1.843 ± 0.345
0.638TyrMet: 0.638 ± 0.202
0.851TyrAsn: 0.851 ± 0.219
0.709TyrPro: 0.709 ± 0.217
0.922TyrGln: 0.922 ± 0.213
2.907TyrArg: 2.907 ± 0.51
1.063TyrSer: 1.063 ± 0.26
0.78TyrThr: 0.78 ± 0.226
2.269TyrVal: 2.269 ± 0.497
0.638TyrTrp: 0.638 ± 0.208
0.496TyrTyr: 0.496 ± 0.216
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 82 proteins (14106 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski