Stenotrophomonas phage vB_SmaS_BUCT548

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 102 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D2HGZ4|A0A7D2HGZ4_9CAUD Putative terminase small subunit OS=Stenotrophomonas phage vB_SmaS_BUCT548 OX=2712941 PE=4 SV=1
MM1 pKa = 7.44NIVATIKK8 pKa = 10.3VCQDD12 pKa = 2.42EE13 pKa = 4.52DD14 pKa = 3.78GNYY17 pKa = 9.9FVMSEE22 pKa = 4.43IPLSDD27 pKa = 3.34QLLYY31 pKa = 11.11SVVTEE36 pKa = 4.69HH37 pKa = 7.43GDD39 pKa = 3.52NLADD43 pKa = 4.9AIEE46 pKa = 4.05EE47 pKa = 4.22AFPSKK52 pKa = 10.95DD53 pKa = 3.48PTLHH57 pKa = 6.25

Molecular weight: 6.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D2LI04|A0A7D2LI04_9CAUD Major head protein OS=Stenotrophomonas phage vB_SmaS_BUCT548 OX=2712941 PE=4 SV=1
MM1 pKa = 7.96RR2 pKa = 11.84IYY4 pKa = 11.15NFFRR8 pKa = 11.84RR9 pKa = 11.84DD10 pKa = 2.99QYY12 pKa = 11.95GRR14 pKa = 11.84LTIIDD19 pKa = 3.63GRR21 pKa = 11.84IGRR24 pKa = 11.84LNFDD28 pKa = 3.81LVHH31 pKa = 5.86YY32 pKa = 7.63PIRR35 pKa = 11.84FKK37 pKa = 11.13HH38 pKa = 5.8NPFFQAYY45 pKa = 8.27KK46 pKa = 10.51SRR48 pKa = 11.84LFGYY52 pKa = 6.8TQISAGKK59 pKa = 9.5LALRR63 pKa = 11.84WLWGEE68 pKa = 4.07KK69 pKa = 10.21

Molecular weight: 8.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

102

0

102

19129

22

2557

187.5

20.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.593 ± 0.492

1.072 ± 0.179

6.064 ± 0.183

6.451 ± 0.37

3.78 ± 0.141

7.669 ± 0.392

1.845 ± 0.214

5.463 ± 0.189

5.175 ± 0.385

7.308 ± 0.287

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.398 ± 0.204

4.909 ± 0.286

4.292 ± 0.248

3.727 ± 0.263

6.163 ± 0.232

5.322 ± 0.299

6.095 ± 0.407

6.801 ± 0.239

1.621 ± 0.137

3.252 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski